LOCUS       V041007                10211 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V041007
VERSION     V041007
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..5037
                     /codon_start=1
                     /product="DNA methyltransferase 3A"
                     /transl_table=1
                     /translation="MPAMPSSGPGDTSSSAAEREEDRKDGEEQEEPRGKEERQEPSTTA
                     RKVGRPGRKRKHPPVESGDTPKDPAVISKSPSMAQDSGASELLPNGDLEKRSEPQPEEG
                     SPAGGQKGGAPAEGEGAAETLPEASRAVENGCCTPKEGRGAPAEAGKEQKETNIESMKM
                     EGSRGRLRGGLGWESSLRQRPMPRLTFQAGDPYYISKRKRDEWLARWKREAEKKAKVIA
                     GMNAVEENQGPGESQKVEEASPPAVQQPTDPASPTVATTPEPVGSDAGDKNATKAGDDE
                     PEYEDGRGFGIGELVWGKLRGFSWWPGRIVSWWMTGRSRAAEGTRWVMWFGDGKFSVVC
                     VEKLMPLSSFCSAFHQATYNKQPMYRKAIYEVLQVASSRAGKLFPVCHDSDESDTAKAV
                     EVQNKPMIEWALGGFQPSGPKGLEPPEEEKNPYKEVYTDMWVEPEAAAYAPPPPAKKPR
                     KSTAEKPKVKEIIDERTRERLVYEVRQKCRNIEDICISCGSLNVTLEHPLFVGGMCQNC
                     KNCFLECAYQYDDDGYQSYCTICCGGREVLMCGNNNCCRCFCVECVDLLVGPGAAQAAI
                     KEDPWNCYMCGHKGTYGLLRRREDWPSRLQMFFANNHDQEFDPPKVYPPVPAEKRKPIR
                     VLSLFDGIATGLLVLKDLGIQVDRYIASEVCEDSITVGMVRHQGKIMYVGDVRSVTQKH
                     IQEWGPFDLVIGGSPCNDLSIVNPARKGLYEGTGRLFFEFYRLLHDARPKEGDDRPFFW
                     LFENVVAMGVSDKRDISRFLESNPVMIDAKEVSAAHRARYFWGNLPGMNRPLASTVNDK
                     LELQECLEHGRIAKFSKVRTITTRSNSIKQGKDQHFPVFMNEKEDILWCTEMERVFGFP
                     VHYTDVSNMSRLARQRLLGRSWSVPVIRHLFAPLKEYFACV"
                     /label="DNMT3A"
     CDS             5044..5067
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin®, an engineered
                     form of streptavidin"
                     /transl_table=1
                     /translation="WSHPQFEK"
                     /label="Strep-Tag II"
     CDS             5107..5130
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin®, an engineered
                     form of streptavidin"
                     /transl_table=1
                     /translation="WSHPQFEK"
                     /label="Strep-Tag II"
     promoter        5181..5392
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5405..5673
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5700..6299
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6306..6894
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6968..7201
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7273..7394
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7413..7548
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7586..7602)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7610..7626
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7634..7664)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7679..7700
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7988..8576)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8747..9607)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9608..9712)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10186..10202
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catgcccgcc atgccctcca gcggccccgg ggacaccagc
     2341 agctctgctg cggagcggga ggaggaccga aaggacggag aggagcagga ggagccgcgt
     2401 ggcaaggagg agcgccaaga gcccagcacc acggcacgga aggtggggcg gcctgggagg
     2461 aagcgcaagc accccccggt ggaaagcggt gacacgccaa aggaccctgc ggtgatctcc
     2521 aagtccccat ccatggccca ggactcaggc gcctcagagc tattacccaa tggggacttg
     2581 gagaagcgga gtgagcccca gccagaggag gggagccctg ctggggggca gaagggcggg
     2641 gccccagcag agggagaggg tgcagctgag accctgcctg aagcctcaag agcagtggaa
     2701 aatggctgct gcacccccaa ggagggccga ggagcccctg cagaagcggg caaagaacag
     2761 aaggagacca acatcgaatc catgaaaatg gagggctccc ggggccggct gcggggtggc
     2821 ttgggctggg agtccagcct ccgtcagcgg cccatgccga ggctcacctt ccaggcgggg
     2881 gacccctact acatcagcaa gcgcaagcgg gacgagtggc tggcacgctg gaaaagggag
     2941 gctgagaaga aagccaaggt cattgcagga atgaatgctg tggaagaaaa ccaggggccc
     3001 ggggagtctc agaaggtgga ggaggccagc cctcctgctg tgcagcagcc cactgacccc
     3061 gcatccccca ctgtggctac cacgcctgag cccgtggggt ccgatgctgg ggacaagaat
     3121 gccaccaaag caggcgatga cgagccagag tacgaggacg gccggggctt tggcattggg
     3181 gagctggtgt gggggaaact gcggggcttc tcctggtggc caggccgcat tgtgtcttgg
     3241 tggatgacgg gccggagccg agcagctgaa ggcacccgct gggtcatgtg gttcggagac
     3301 ggcaaattct cagtggtgtg tgttgagaag ctgatgccgc tgagctcgtt ttgcagtgcg
     3361 ttccaccagg ccacgtacaa caagcagccc atgtaccgca aagccatcta cgaggtcctg
     3421 caggtggcca gcagccgcgc ggggaagctg ttcccggtgt gccacgacag cgatgagagt
     3481 gacactgcca aggccgtgga ggtgcagaac aagcccatga ttgaatgggc cctggggggc
     3541 ttccagcctt ctggccctaa gggcctggag ccaccagaag aagagaagaa tccctacaaa
     3601 gaagtgtaca cggacatgtg ggtggaacct gaggcagctg cctacgcacc acctccacca
     3661 gccaaaaagc cccggaagag cacagcggag aagcccaagg tcaaggagat tattgatgag
     3721 cgcacaagag agcggctggt gtacgaggtg cggcagaagt gccggaacat tgaggacatc
     3781 tgcatctcct gtgggagcct caatgttacc ctggaacacc ccctcttcgt tggaggaatg
     3841 tgccaaaact gcaagaactg ctttctggag tgtgcgtacc agtacgacga cgacggctac
     3901 cagtcctact gcaccatctg ctgtgggggc cgtgaggtgc tcatgtgcgg aaacaacaac
     3961 tgctgcaggt gcttttgcgt ggagtgtgtg gacctcttgg tggggccggg ggctgcccag
     4021 gcagccatta aggaagaccc ctggaactgc tacatgtgcg ggcacaaggg tacctacggg
     4081 ctgctgcggc ggcgagagga ctggccctcc cggctccaga tgttcttcgc taataaccac
     4141 gaccaggaat ttgaccctcc aaaggtttac ccacctgtcc cagctgagaa gaggaagccc
     4201 atccgggtgc tgtctctctt tgatggaatc gctacagggc tcctggtgct gaaggacttg
     4261 ggcattcagg tggaccgcta cattgcctcg gaggtgtgtg aggactccat cacggtgggc
     4321 atggtgcggc accaggggaa gatcatgtac gtcggggacg tccgcagcgt cacacagaag
     4381 catatccagg agtggggccc attcgatctg gtgattgggg gcagtccctg caatgacctc
     4441 tccatcgtca accctgctcg caagggcctc tacgagggca ctggccggct cttctttgag
     4501 ttctaccgcc tcctgcatga tgcgcggccc aaggagggag atgatcgccc cttcttctgg
     4561 ctctttgaga atgtggtggc catgggcgtt agtgacaaga gggacatctc gcgatttctc
     4621 gagtccaacc ctgtgatgat tgatgccaaa gaagtgtcag ctgcacacag ggcccgctac
     4681 ttctggggta accttcccgg tatgaacagg ccgttggcat ccactgtgaa tgataagctg
     4741 gagctgcagg agtgtctgga gcatggcagg atagccaagt tcagcaaagt gaggaccatt
     4801 actacgaggt caaactccat aaagcagggc aaagaccagc attttcctgt cttcatgaat
     4861 gagaaagagg acatcttatg gtgcactgaa atggaaaggg tatttggttt cccagtccac
     4921 tatactgacg tctccaacat gagccgcttg gcgaggcaga gactgctggg ccggtcatgg
     4981 agcgtgccag tcatccgcca cctcttcgct ccgctgaagg agtattttgc gtgtgtgctc
     5041 gagtggagcc atccgcagtt tgaaaaaggt ggtggtagcg gtggtggttc aggtggtagc
     5101 agtgcatggt cacaccctca gtttgagaaa tgaggatccg cggccgcgaa ggatctgcga
     5161 tcgctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg
     5221 gggaggggtc ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa actgggaaag
     5281 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc
     5341 agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac agctgaagct
     5401 tcgaggggct cgcatctctc cttcacgcgc ccgccgccct acctgaggcc gccatccacg
     5461 ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg cgtccgccgt
     5521 ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc cttggagcct
     5581 acctagactc agccggctct ccacgctttg cctgaccctg cttgctcaac tctacgtctt
     5641 tgtttcgttt tctgttctgc gccgttacag atccaagctg tgaccggcgc ctacgctaga
     5701 tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc cgggccgtac
     5761 gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gacccggacc
     5821 gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca
     5881 tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga
     5941 gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt
     6001 cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc
     6061 ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca
     6121 gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg
     6181 agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg
     6241 acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgag
     6301 tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     6361 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     6421 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     6481 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     6541 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     6601 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     6661 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc
     6721 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     6781 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     6841 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggta
     6901 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     6961 gggggactgg aagggctaat tcactcccaa cgaaaataag atctgctttt tgcttgtact
     7021 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     7081 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     7141 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     7201 agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca
     7261 gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     7321 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     7381 atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg cccagttccg
     7441 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     7501 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta gacttttgca
     7561 gagacggccc aaattcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     7621 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     7681 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     7741 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     7801 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     7861 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     7921 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     7981 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     8041 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     8101 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     8161 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     8221 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     8281 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     8341 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     8401 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
     8461 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     8521 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     8581 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     8641 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     8701 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     8761 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     8821 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     8881 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     8941 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     9001 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     9061 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     9121 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     9181 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     9241 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     9301 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     9361 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     9421 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     9481 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     9541 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     9601 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     9661 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     9721 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
     9781 ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct
     9841 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
     9901 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg
     9961 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg
    10021 taaggagaaa ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag
    10081 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
    10141 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
    10201 gtgccaagct g
//
