LOCUS       V025610                10883 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V025610
VERSION     V025610
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4926
                     /label="DLG2(NM_001364)"
                     /note="DLG2(NM_001364)"
                     /gene="DLG2"
     CDS             4933..4998
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5042..5253
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5266..5534
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5563..5572
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5593..6255
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6325..6378
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6379..6978
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6979..7566
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7640..7873
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7945..8066
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8085..8220
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8258..8274)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8282..8298
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8306..8336)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8351..8372
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8660..9248)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9419..10279)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10280..10384)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10858..10874
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgt tctttgcatg ttactgtgca
     2341 ctccggacta acgtgaagaa gtatcgatat caagatgagg acgctccaca tgatcattcc
     2401 ttacctcgac taacccacga agtaagaggc ccagaactcg tgcatgtatc agaaaagaac
     2461 ctctctcaaa tagaaaatgt ccatggatat gtcttgcagt ctcatatttc tcctctgaag
     2521 gccagtcctg ctcctataat tgtcaacaca gatactttgg acacaattcc ttatgtcaat
     2581 gggacagaaa ttgaatatga atttgaagaa attacactgg agagggggaa ttctggcctg
     2641 ggattcagta ttgctggggg gacagataat ccccacattg gagatgaccc tggcatattt
     2701 attacgaaga ttataccagg aggtgctgca gcagaggatg gcagactcag ggtcaatgat
     2761 tgtatcttgc gggtgaatga ggttgatgtg tcagaggttt cccacagtaa agcggtggaa
     2821 gccctgaagg aagcagggtc tatcgttcgg ctgtatgtgc gtagaagacg acctattttg
     2881 gagaccgttg tggaaatcaa actgttcaaa ggccctaaag gtttaggctt cagtattgca
     2941 ggaggtgtgg ggaaccaaca cattcctgga gacaacagca tttatgtaac taaaattata
     3001 gatggaggag ctgcacaaaa agatggaagg ttgcaagtag gagatagact actaatggta
     3061 aacaactaca gtttagaaga agtaacacac gaagaggcag tagcaatatt aaagaacaca
     3121 tcagaggtag tttatttaaa agttggcaaa cccactacca tttatatgac tgatccttat
     3181 ggtccacctg atattactca ctcttattct ccaccaatgg aaaaccatct actctctggc
     3241 aacaatggca ctttagaata taaaacctcc ctgccaccca tctctccagg aaggtactca
     3301 ccaattccaa agcacatgct tgttgacgac gactacacca ggcctccgga acctgtttac
     3361 agcactgtga acaaactatg tgataagcct gcttctccca ggcactattc ccctgttgag
     3421 tgtgacaaaa gcttcctcct ctcagctccc tattcccact accacctagg cctgctacct
     3481 gactctgaga tgaccagtca ttcccaacat agcaccgcaa ctcgtcagcc ttcaatgact
     3541 ctccaacggg ccgtctccct ggaaggagag cctcgcaagg tagtcctgca caaaggctcc
     3601 actggcctgg gcttcaacat tgtcggtggg gaagatggag aaggtatttt tgtgtccttc
     3661 attctggctg gtggaccagc agacctaagt ggggagctcc agagaggaga ccagatccta
     3721 tcggtgaatg gcattgacct ccgtggtgca tcccacgagc aggcagctgc tgcactaaag
     3781 ggggctggac agacagtgac gattatagca caatatcaac ctgaagatta cgctcgattt
     3841 gaggccaaaa tccatgacct acgagagcag atgatgaacc acagcatgag ctccgggtcc
     3901 ggatccctgc gaaccaatca gaaacgctcc ctctacgtca gagccatgtt cgactacgac
     3961 aagagcaagg acagtgggct gccaagtcaa ggacttagtt ttaaatatgg agatattctc
     4021 cacgttatca atgcctctga tgatgagtgg tggcaagcca ggagagtcat gctggaggga
     4081 gacagtgagg agatgggggt catccccagc aaaaggaggg tggaaagaaa ggaacgtgcc
     4141 cgattgaaga cagtgaagtt taatgccaaa cctggagtga ttgattcgaa agggtcattc
     4201 aatgacaagc gtaaaaagag cttcatcttt tcacgaaaat tcccattcta caagaacaag
     4261 gagcagagtg agcaggaaac cagtgatcct gaacgaggac aagaagacct cattctttcc
     4321 tatgagcctg ttacaaggca ggaaataaac tacacccggc cggtgattat cctggggccc
     4381 atgaaggatc ggatcaatga cgacttgata tctgaattcc ctgataaatt tggctcctgt
     4441 gtgcctcata ctacgaggcc aaagcgagac tacgaggtgg atggcagaga ctatcacttt
     4501 gtcatttcca gagaacaaat ggagaaagat atccaagagc acaagtttat agaagccggc
     4561 cagtacaatg acaatttata tggaaccagt gtgcagtctg tgagatttgt agcagaaaga
     4621 ggcaaacact gtatacttga tgtatcagga aatgctatca agcggttaca agttgcccag
     4681 ctctatccca ttgccatctt cataaaaccc aggtctctgg aacctcttat ggagatgaat
     4741 aagcgtctaa cagaggaaca agccaagaaa acctatgatc gagcaattaa gctagaacaa
     4801 gaatttggag aatattttac agctattgtc caaggagata ctttagaaga tatatataac
     4861 caatgcaagc ttgttattga agagcaatct gggcctttca tctggattcc ctcaaaggaa
     4921 aagttaggat ccgactacaa agaccatgac ggtgattata aagatcatga catcgactac
     4981 aaggatgacg atgacaagta gcggccgcga aggatctgcg atcgctccgg tgcccgtcag
     5041 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga
     5101 acgggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc
     5161 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt
     5221 ctttttcgca acgggtttgc cgccagaaca cagctgaagc ttcgaggggc tcgcatctct
     5281 ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg
     5341 ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc
     5401 aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc
     5461 tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg
     5521 cgccgttaca gatccaagct gtgaccggcg cctacgctag acgccaccat ggagagcgac
     5581 gagagcggcc tgcccgccat ggagatcgag tgccgcatca ccggcaccct gaacggcgtg
     5641 gagttcgagc tggtgggcgg cggagagggc acccccaagc agggccgcat gaccaacaag
     5701 atgaagagca ccaaaggcgc cctgaccttc agcccctacc tgctgagcca cgtgatgggc
     5761 tacggcttct accacttcgg cacctacccc agcggctacg agaacccctt cctgcacgcc
     5821 atcaacaacg gcggctacac caacacccgc atcgagaagt acgaggacgg cggcgtgctg
     5881 cacgtgagct tcagctaccg ctacgaggcc ggccgcgtga tcggcgactt caaggtggtg
     5941 ggcaccggct tccccgagga cagcgtgatc ttcaccgaca agatcatccg cagcaacgcc
     6001 accgtggagc acctgcaccc catgggcgat aacgtgctgg tgggcagctt cgcccgcacc
     6061 ttcagcctgc gcgacggcgg ctactacagc ttcgtggtgg acagccacat gcacttcaag
     6121 agcgccatcc accccagcat cctgcagaac gggggcccca tgttcgcctt ccgccgcgtg
     6181 gaggagctgc acagcaacac cgagctgggc atcgtggagt accagcacgc cttcaagacc
     6241 cccatcgcct tcgccagatc ccgcgctcag tcgtccaatt ctgccgtgga cggcaccgcc
     6301 ggacccggct ccaccggatc tcgcgagggc agaggaagtc ttctaacatg cggtgacgtg
     6361 gaggagaatc ccggccctat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
     6421 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
     6481 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
     6541 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
     6601 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
     6661 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
     6721 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac
     6781 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
     6841 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc
     6901 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
     6961 cgcaagcccg gtgcctgaaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     7021 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     7081 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     7141 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     7201 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     7261 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     7321 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg
     7381 tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     7441 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     7501 cggcctcttc cgcgtctccg ccttcgccct cagacgagtc ggatctccct ttggccgcct
     7561 ccccgcctgg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     7621 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaaaata agatctgctt
     7681 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     7741 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     7801 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     7861 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag
     7921 aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa
     7981 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     8041 ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc
     8101 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     8161 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     8221 tagacttttg cagagacggc ccaaattcgt aatcatggtc atagctgttt cctgtgtgaa
     8281 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     8341 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     8401 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     8461 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     8521 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     8581 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     8641 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     8701 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     8761 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     8821 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     8881 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     8941 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     9001 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     9061 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
     9121 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     9181 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     9241 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     9301 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     9361 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     9421 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     9481 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     9541 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     9601 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     9661 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     9721 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     9781 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     9841 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     9901 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     9961 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
    10021 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
    10081 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
    10141 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
    10201 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
    10261 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
    10321 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
    10381 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat
    10441 taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg
    10501 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg
    10561 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc
    10621 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac
    10681 cgcacagatg cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca
    10741 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg
    10801 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
    10861 aaacgacggc cagtgccaag ctg
//
