LOCUS       V028364                10119 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V028364
VERSION     V028364
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1798..1915
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2005..2208
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2297..4138
                     /label="DDX5(NM_001320595)"
                     /note="DDX5(NM_001320595)"
                     /gene="DDX5"
     CDS             4145..4234
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /transl_table=1
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
                     /label="3xMyc"
     promoter        4278..4489
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4502..4770
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      4799..4808
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             4829..5491
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5561..5614
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5615..6214
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6215..6802
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6876..7109
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7181..7302
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7321..7456
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7494..7510)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7518..7534
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7542..7572)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7587..7608
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7896..8484)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8655..9515)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9516..9620)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10094..10110
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc
     2281 tagcgaattc gccaccatgt cgggttattc gagtgaccga gaccgcggcc gggaccgagg
     2341 gtttggtgca cctcgatttg gaggaagtag ggcagggccc ttatctggaa agaagtttgg
     2401 aaaccctggg gagaaattag ttaaaaagaa gtggaatctt gatgagctgc ctaaatttga
     2461 gaagaatttt tatcaagagc accctgattt ggctaggcgc acagcacaag aggtggaaac
     2521 atacagaaga agcaaggaaa ttacagttag aggtcacaac tgcccgaagc cagttctaaa
     2581 tttttatgaa gccaatttcc ctgcaaatgt catggatgtt attgcaagac agaatttcac
     2641 tgaacccact gctattcaag ctcagggatg gccagttgct ctaagtggat tggatatggt
     2701 tggagtggca cagactggat ctgggaaaac attgtcttat ttgcttcctg ccattgtcca
     2761 catcaatcat cagccattcc tagagagagg cgatgggcct atttgtttgg tgctggcacc
     2821 aactcgggaa ctggcccaac aggtgcagca agtagctgct gaatattgta gagcatgtcg
     2881 cttgaagtct acttgtatct acggtggtgc tcctaaggga ccacaaatac gtgatttgga
     2941 gagaggtgtg gaaatctgta ttgcaacacc tggaagactg attgactttt tagagtgtgg
     3001 aaaaaccaat ctgagaagaa caacctacct tgtccttgat gaagcagata gaatgcttga
     3061 tatgggcttt gaaccccaaa taaggaagat tgtggatcaa ataagacctg ataggcaaac
     3121 tctaatgtgg agtgcgactt ggccaaaaga agtaagacag cttgctgaag atttcctgaa
     3181 agactatatt catataaaca ttggtgcact tgaactgagt gcaaaccaca acattcttca
     3241 gattgtggat gtgtgtcatg acgtagaaaa ggatgaaaaa cttattcgtc taatggaaga
     3301 gatcatgagt gagaaggaga ataaaaccat tgtttttgtg gaaaccaaaa gaagatgtga
     3361 tgagcttacc agaaaaatga ggagagatgg gtggcctgcc atgggtatcc atggtgacaa
     3421 gagtcaacaa gagcgtgact gggttctaaa tgaattcaaa catggaaaag ctcctattct
     3481 gattgctaca gatgtggcct ccagagggct agatgtggaa gatgtgaaat ttgtcatcaa
     3541 ttatgactac cctaactcct cagaggatta tattcatcga attggaagaa ctgctcgcag
     3601 taccaaaaca ggcacagcat acactttctt tacacctaat aacataaagc aagtgagcga
     3661 ccttatctct gtgcttcgtg aagctaatca agcaattaat cccaagttgc ttcagttggt
     3721 cgaagacaga ggttcaggtc gttccagggg tagaggaggc atgaaggatg accgtcggga
     3781 cagatactct gcgggcaaaa ggggtggatt taataccttt agagacaggg aaaattatga
     3841 cagaggttac tctagcctgc ttaaaagaga ttttggggca aaaactcaga atggtgttta
     3901 cagtgctgca aattacacca atgggagctt tggaagtaat tttgtgtctg ctggtataca
     3961 gaccagtttt aggactggta atccaacagg gacttaccag aatggttatg atagcactca
     4021 gcaatacgga agtaatgttc caaatatgca caatggtatg aaccaacagg catatgcata
     4081 tcctgctact gcagctgcac ctatgattgg ttatccaatg ccaacaggat attcccaagg
     4141 atccgaacaa aaactaatat cagaggaaga tttggaacag aaactaatca gtgaagaaga
     4201 tttagagcaa aagttaattt ccgaggagga cttatagcgg ccgcgaagga tctgcgatcg
     4261 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg
     4321 aggggtcggc aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga
     4381 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt
     4441 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg
     4501 aggggctcgc atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg
     4561 gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta
     4621 ggtaagttta aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc
     4681 tagactcagc cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt
     4741 ttcgttttct gttctgcgcc gttacagatc caagctgtga ccggcgccta cgctagacgc
     4801 caccatggag agcgacgaga gcggcctgcc cgccatggag atcgagtgcc gcatcaccgg
     4861 caccctgaac ggcgtggagt tcgagctggt gggcggcgga gagggcaccc ccaagcaggg
     4921 ccgcatgacc aacaagatga agagcaccaa aggcgccctg accttcagcc cctacctgct
     4981 gagccacgtg atgggctacg gcttctacca cttcggcacc taccccagcg gctacgagaa
     5041 ccccttcctg cacgccatca acaacggcgg ctacaccaac acccgcatcg agaagtacga
     5101 ggacggcggc gtgctgcacg tgagcttcag ctaccgctac gaggccggcc gcgtgatcgg
     5161 cgacttcaag gtggtgggca ccggcttccc cgaggacagc gtgatcttca ccgacaagat
     5221 catccgcagc aacgccaccg tggagcacct gcaccccatg ggcgataacg tgctggtggg
     5281 cagcttcgcc cgcaccttca gcctgcgcga cggcggctac tacagcttcg tggtggacag
     5341 ccacatgcac ttcaagagcg ccatccaccc cagcatcctg cagaacgggg gccccatgtt
     5401 cgccttccgc cgcgtggagg agctgcacag caacaccgag ctgggcatcg tggagtacca
     5461 gcacgccttc aagaccccca tcgccttcgc cagatcccgc gctcagtcgt ccaattctgc
     5521 cgtggacggc accgccggac ccggctccac cggatctcgc gagggcagag gaagtcttct
     5581 aacatgcggt gacgtggagg agaatcccgg ccctatgacc gagtacaagc ccacggtgcg
     5641 cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga
     5701 ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct
     5761 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga
     5821 cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc
     5881 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat
     5941 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg
     6001 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga
     6061 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc
     6121 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg
     6181 cacctggtgc atgacccgca agcccggtgc ctgaaatcaa cctctggatt acaaaatttg
     6241 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     6301 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     6361 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     6421 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     6481 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     6541 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     6601 gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg
     6661 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     6721 cctgctgccg gctctgcggc ctcttccgcg tctccgcctt cgccctcaga cgagtcggat
     6781 ctccctttgg ccgcctcccc gcctggtacc tttaagacca atgacttaca aggcagctgt
     6841 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
     6901 aaaataagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
     6961 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     7021 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     7081 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta
     7141 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta
     7201 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     7261 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct
     7321 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     7381 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     7441 ggcttttttg gaggcctaga cttttgcaga gacggcccaa attcgtaatc atggtcatag
     7501 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     7561 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     7621 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     7681 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     7741 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     7801 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     7861 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     7921 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     7981 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     8041 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     8101 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     8161 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     8221 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     8281 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
     8341 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     8401 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     8461 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     8521 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     8581 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     8641 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     8701 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     8761 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     8821 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     8881 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     8941 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     9001 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     9061 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     9121 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     9181 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     9241 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     9301 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     9361 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     9421 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     9481 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     9541 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     9601 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
     9661 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg
     9721 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
     9781 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
     9841 gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat
     9901 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg
     9961 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
    10021 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc
    10081 cagtcacgac gttgtaaaac gacggccagt gccaagctg
//
