LOCUS       V032265                11008 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V032265
VERSION     V032265
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..5034
                     /label="DDX11(NM_030653)"
                     /note="DDX11(NM_030653)"
                     /gene="DDX11"
     CDS             5041..5106
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5150..5361
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5374..5642
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5671..5680
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5701..6363
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6433..6486
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6487..7086
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7087..7675
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7749..7982
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8054..8175
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8215..8350
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8383..8399)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8407..8423
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8431..8461)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8476..8497
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8785..9373)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9544..10404)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10405..10509)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10983..10999
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg ctaatgaaac acagaaggtt
     2341 ggtgccatcc attttccttt tcccttcaca ccctattcca tccaggaaga cttcatggca
     2401 gagctgtacc gggttttgga ggctggcaag attgggatat ttgagagtcc aactggcact
     2461 gggaagtcct taagtcttat ttgtggggcc ctctcttggc tccgtgactt tgaacagaag
     2521 aagcgtgaag aagaggcacg actccttgaa actggaactg gccccttaca tgatgagaaa
     2581 gatgaatccc tgtgtctgtc ttcttcctgc gaaggggctg caggcacccc gaggcctgct
     2641 ggagaaccgg cctgggttac tcagtttgtg cagaagaaag aagagaggga cctggtggac
     2701 cgactaaagg cggagcaggc caggaggaag cagcgagaag aacgcctgca gcagctgcag
     2761 cacagggtgc agctcaagta tgcagccaag cgcctgaggc aggaagaaga agaaagagag
     2821 aatctcctcc gcctcagcag ggagatgcta gagacaggcc cggaggctga gcggctggag
     2881 cagctggagt ctggggagga ggagctggtc ctcgccgaat acgagagtga tgaggagaaa
     2941 aaggtggcga gcagagtgga tgaggatgag gatgacctgg aggaagaaca cataactaag
     3001 atttattact gtagtcggac acactcccag ctggcccagt ttgtgcatga ggtgaagaag
     3061 agcccctttg gcaaggatgt tcggctggtc tcccttggct cccggcagaa cctttgtgta
     3121 aatgaagacg tgaaaagcct aggttctgtg cagcttatca acgaccgctg tgtggacatg
     3181 cagagaagca ggcacgagaa gaagaaagga gctgaggagg agaagccaaa gaggaggagg
     3241 caggagaagc aggcagcctg ccccttctac aaccacgagc agatgggcct tctccgggat
     3301 gaggccctgg cagaggtgaa ggacatggag cagctgctgg cccttgggaa ggaggcccgg
     3361 gcctgtccct attacgggag ccgccttgcc atccctgcag cccagctggt ggtgctgccc
     3421 tatcagatgc tgctgcatgc ggccactcgg caggccgcgg gcatccggct gcaggaccag
     3481 gtggtgatca tcgacgaggc gcacaacctg atcgacacca tcacgggcat gcacagcgtg
     3541 gaggtcagcg gctcccagct ctgccaggcc cattcccagc tgctgcagta cgtggagcga
     3601 tacgggaagc gtttgaaggc caagaacctg atgtacctga agcagatcct gtatttgctg
     3661 gagaaattcg tggctgtgct aggggggaac attaagcaaa atcccaatac acagagtctg
     3721 tcacagacag ggacggagct gaagaccatc aacgactttc tcttccagag ccagatcgac
     3781 aacatcaacc tgttcaaggt gcagcgatac tgtgagaaga gcatgatcag cagaaagctc
     3841 tttggattca ctgaacggta cggagcagtg ttctcatccc gggagcagcc caaactggct
     3901 gggtttcagc aattcctgca gagcctgcag cccaggacga ctgaagctct tgcagcccct
     3961 gcagacgaga gtcaggccag caccctgcga ccagcttctc cactgatgca catccaaggc
     4021 ttcctggcag ctctcactac ggccaaccag gacggcaggg tcatcctgag ccgccaaggc
     4081 agcctcagtc agagcaccct gaagtttttg ctcctgaatc cagctgtgca ctttgcccaa
     4141 gtggtgaagg aatgccgggc agtggtcatt gcggggggta ccatgcagcc ggtgtctgac
     4201 ttccggcagc agctgctggc ctgtgccggg gtggaagctg agcgcgtggt ggagttttcc
     4261 tgtggtcacg tgatccctcc agacaacatc ctgcccctcg tcatctgcag cgggatctcc
     4321 aaccagccgc tggaattcac gttccagaaa agagagctgc ctcagatgat ggacgaggtg
     4381 ggtcgcattc tctgtaacct gtgcggtgtg gttcctggag gggtggtctg tttcttcccc
     4441 tcctacgagt acctgcgcca ggtccatgcc cactgggaga agggtggcct gctgggccgt
     4501 ctggctgcca ggaagaagat attccaggaa cctaagagcg cacaccaggt ggagcaggtg
     4561 ctgctggcat attccaggtg catccaggcc tgtggccagg agagaggcca ggtgacaggg
     4621 gccctgctcc tctctgtggt tggaggaaag atgagtgaag ggatcaactt ctctgacaac
     4681 ctaggccggt gtgtggtgat ggtgggcatg cccttcccca acatcaggtc tgcagagctg
     4741 caggagaaga tggcctactt ggatcaaacc ctccccagag cccccggcca ggcaccccca
     4801 gggaaggctc tggtggagaa cctgtgcatg aaggccgtca accagtccat aggcagggcc
     4861 atcaggcacc agaaggattt tgccagcgta gtgctcctgg accagcgata tgcccggccc
     4921 cctgtcctgg ccaagctgcc ggcctggatc cgagcccgtg tggaggtcaa agctaccttt
     4981 ggccccgcca ttgctgctgt gcagaagttt caccgggaga agtcggcctc ttccggatcc
     5041 gactacaaag accatgacgg tgattataaa gatcatgaca tcgactacaa ggatgacgat
     5101 gacaagtagc ggccgcgaag gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc
     5161 acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac gggtgcctag
     5221 agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc
     5281 gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac
     5341 gggtttgccg ccagaacaca gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc
     5401 cgccgcccta cctgaggccg ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc
     5461 tgtggtgcct cctgaactgc gtccgccgtc taggtaagtt taaagctcag gtcgagaccg
     5521 ggcctttgtc cggcgctccc ttggagccta cctagactca gccggctctc cacgctttgc
     5581 ctgaccctgc ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga
     5641 tccaagctgt gaccggcgcc tacgctagac gccaccatgg agagcgacga gagcggcctg
     5701 cccgccatgg agatcgagtg ccgcatcacc ggcaccctga acggcgtgga gttcgagctg
     5761 gtgggcggcg gagagggcac ccccaagcag ggccgcatga ccaacaagat gaagagcacc
     5821 aaaggcgccc tgaccttcag cccctacctg ctgagccacg tgatgggcta cggcttctac
     5881 cacttcggca cctaccccag cggctacgag aaccccttcc tgcacgccat caacaacggc
     5941 ggctacacca acacccgcat cgagaagtac gaggacggcg gcgtgctgca cgtgagcttc
     6001 agctaccgct acgaggccgg ccgcgtgatc ggcgacttca aggtggtggg caccggcttc
     6061 cccgaggaca gcgtgatctt caccgacaag atcatccgca gcaacgccac cgtggagcac
     6121 ctgcacccca tgggcgataa cgtgctggtg ggcagcttcg cccgcacctt cagcctgcgc
     6181 gacggcggct actacagctt cgtggtggac agccacatgc acttcaagag cgccatccac
     6241 cccagcatcc tgcagaacgg gggccccatg ttcgccttcc gccgcgtgga ggagctgcac
     6301 agcaacaccg agctgggcat cgtggagtac cagcacgcct tcaagacccc catcgccttc
     6361 gccagatccc gcgctcagtc gtccaattct gccgtggacg gcaccgccgg acccggctcc
     6421 accggatctc gcgagggcag aggaagtctt ctaacatgcg gtgacgtgga ggagaatccc
     6481 ggccctatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg
     6541 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat
     6601 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg
     6661 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg
     6721 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg
     6781 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc
     6841 aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt
     6901 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc
     6961 ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc
     7021 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt
     7081 gcctgaaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     7141 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     7201 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     7261 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     7321 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     7381 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     7441 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     7501 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     7561 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     7621 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctggt
     7681 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     7741 ggggggactg gaagggctaa ttcactccca acgaagataa gatctgcttt ttgcttgtac
     7801 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     7861 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     7921 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     7981 cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc
     8041 agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     8101 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     8161 catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg
     8221 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     8281 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     8341 tttggaggcc tagacttttg cagagaccaa attcgtaatc atgtcatagc tgtttcctgt
     8401 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     8461 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     8521 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     8581 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     8641 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     8701 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     8761 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     8821 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     8881 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     8941 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     9001 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     9061 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     9121 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     9181 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     9241 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     9301 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     9361 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     9421 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     9481 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     9541 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     9601 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     9661 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     9721 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     9781 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     9841 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     9901 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     9961 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
    10021 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
    10081 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
    10141 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
    10201 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
    10261 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
    10321 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
    10381 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
    10441 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
    10501 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
    10561 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga
    10621 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
    10681 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
    10741 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga
    10801 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct
    10861 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
    10921 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
    10981 ttgtaaaacg acggccagtg ccaagctg
//
