LOCUS       V029317                12649 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V029317
VERSION     V029317
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..6675
                     /label="Col1a1(NM_007742)"
                     /note="Col1a1(NM_007742)"
                     /gene="Col1a1"
     CDS             6682..6747
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        6791..7002
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             7015..7283
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      7312..7321
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             7342..8004
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             8074..8127
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             8128..8727
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    8728..9316
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             9390..9623
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    9695..9816
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      9856..9991
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(10024..10040)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    10048..10064
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(10072..10102)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    10117..10138
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(10426..11014)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(11185..12045)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(12046..12150)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     12624..12640
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgt tcagctttgt ggacctccgg
     2341 ctcctgctcc tcttaggggc cactgccctc ctgacgcatg gccaagaaga catccctgaa
     2401 gtcagctgca tacacaatgg cctaagggtc cccaatggtg agacgtggaa acccgaggta
     2461 tgcttgatct gtatctgcca caatggcacg gctgtgtgcg atgacgtgca atgcaatgaa
     2521 gaactggact gtcccaaccc ccaaagacgg gagggcgagt gctgtgcttt ctgcccggaa
     2581 gaatacgtat caccaaactc agaagatgta ggagtcgagg gacccaaggg agaccctggc
     2641 ccccaaggcc caaggggacc cgttggcccc cctggacgag atggcatccc tggacagcct
     2701 ggacttcctg gtcctcctgg tccccctggg ccccccggac cccctggcct tggaggaaac
     2761 tttgcttccc agatgtccta tggctatgat gaaaaatcag ctggagtttc cgtgcctggc
     2821 cccatgggtc cttctggtcc tcgtggtctc cctggccccc ctggtgcacc tggtccacaa
     2881 ggtttccaag gcccccctgg tgaacctggc gagcctggcg gttcaggtcc aatgggtccc
     2941 cgaggtcccc ctggccctcc tggcaagaat ggagatgatg gggaagctgg caagcccggc
     3001 cgtcctggtg agcgtggacc tcctggacct cagggtgctc gtggattgcc tggaacagct
     3061 ggcctccctg gaatgaaggg acaccgaggc ttcagtggtt tggatggtgc caaaggagat
     3121 gctggtcctg ctggtcctaa gggagagccc ggcagtcctg gtgaaaacgg agctcctggc
     3181 cagatgggtc cccgaggtct gcccggtgag agaggtcgcc ctggacctcc tggcactgct
     3241 ggtgctcgcg gtaacgatgg tgctgttggt gctgctggac cccctggtcc caccggcccc
     3301 actggccctc ctggcttccc tggtgcagtt ggtgctaagg gtgaagctgg tccccaagga
     3361 gctagaggct ctgaaggtcc ccagggtgtg cgtggtgagc ccggaccccc tggccctgct
     3421 ggtgctgccg gccctgctgg aaaccctggt gctgatggac aacctggcgc taaaggtgcc
     3481 aatggtgctc ctggtattgc tggtgctcct ggcttccctg gtgcccgagg cccctctgga
     3541 ccccagggcc ccagcggccc tccaggtccc aagggtaaca gtggtgaacc tggtgctcct
     3601 ggcaacaaag gagacactgg tgccaaagga gaacccggtg ctactggagt tcaaggtccc
     3661 ccaggccctg ccggagaaga aggaaaacga ggagcccgtg gtgagcctgg accttccgga
     3721 ctgcctggac ctcctggcga gcgtggtgga cctggtagcc gtggtttccc tggtgctgat
     3781 ggtgttgctg gccccaaggg tccttccggt gaacgtggtg ctcccggacc tgctggtccc
     3841 aaaggttctc ctggtgaagc tggtcgcccc ggtgaagctg gtctccctgg tgccaagggt
     3901 ctcactggca gtcctggcag ccctggtcct gatggcaaaa ccggcccccc tggtcccgct
     3961 ggtcaagatg gtcgccctgg acccgcaggt cctcctggag cccgtggcca ggctggtgtg
     4021 atgggattcc ctggacctaa gggtaccgct ggagaacctg gaaaggctgg agagcgaggc
     4081 cttcccggac cccctggcgc tgttggtcct gctggcaaag atggagaagc tggagctcag
     4141 ggagcccctg gccctgctgg tcctgctggt gagagaggtg aacaaggtcc cgctggctcc
     4201 cctggattcc agggtcttcc tggtcctgcc ggtcctcctg gtgaagcagg caagcctggt
     4261 gaacagggtg ttcctggaga ccttggtgcc cctggaccct ctggcgcaag aggcgagaga
     4321 ggtttccctg gtgaacgtgg tgtacaaggt cccccaggtc ctgctggtcc ccgaggaaac
     4381 aatggtgccc ccggcaacga tggtgccaag ggtgatactg gtgcccccgg agctcccggt
     4441 agccagggtg cccccggtct tcagggaatg cctggtgaac gtggtgcagc tggtcttcca
     4501 ggtcctaagg gtgacagagg tgatgctggt cccaaaggtg ctgatggttc tcctggtaaa
     4561 gatggtgccc gtggtctgac tggtcccatt ggtcctcctg gccctgctgg tgcccctggt
     4621 gacaagggtg aagctggtcc cagtggtcct cccggtccca ccggagcccg tggtgctccc
     4681 ggagaccgtg gtgaggctgg tccccctggt cctgctggct ttgccggccc ccctggtgct
     4741 gatggccaac ctggtgcgaa aggtgaacct ggtgatactg gtgttaaagg tgatgctggt
     4801 cctcctggcc ctgctggtcc tgctggaccc cccggcccca ttggtaacgt tggtgctcct
     4861 ggacccaaag gtcctcgtgg tgctgctggt ccccctggtg ctactggctt ccctggtgct
     4921 gctggccgtg tcggtccccc tggtccctct ggaaatgctg gaccccctgg ccctcccggt
     4981 cccgttggca aagaaggggg caaaggtccc cgtggtgaga ctggccctgc tggacgtcct
     5041 ggtgaagttg gtcccccagg tccccccggt cctgctggtg agaaaggatc tcctggtgct
     5101 gatggacctg ctggctctcc tggtacccct ggacctcagg gtattgctgg acaacgtggt
     5161 gtggtcggtc ttcccggtca gagaggagaa agaggcttcc ctggtcttcc tggcccctct
     5221 ggtgaacctg gcaaacaagg tccttctgga tcaagtggtg aacgcggtcc ccctggcccc
     5281 atggggcccc ctggattggc tggtccccct ggtgaatctg gacgtgaggg atcccctggt
     5341 gctgaaggct cccctggaag ggatggtgct cccggggcca agggtgaccg tggtgagact
     5401 ggccccgctg gcccccctgg tgcccctggt gctcccggtg ctcccggccc tgttggtccc
     5461 gctggcaaga atggcgatcg tggtgagact ggtcctgctg gtcctgctgg tcccattggc
     5521 cctgctggtg cccgtggccc tgctggaccc caaggccccc gtggtgacaa gggtgagaca
     5581 ggcgaacaag gtgacagagg cataaagggt catcgtggct tctctggtct ccagggtcct
     5641 cctggttctc ctggttctcc tggtgaacaa ggcccctctg gagcttcagg tcctgcaggc
     5701 ccccggggtc cccctggctc tgctggttct cctggcaaag acggactcaa cggtctccct
     5761 ggccccattg gtccccctgg tcctcgaggt cgcactggtg acagcggccc tgctggtccc
     5821 cccggccctc ctggaccccc tggccctcct ggacctccca gtggcggtta tgacttcagc
     5881 ttcctgcctc agccacctca agagaagtct caagatggtg gccgctacta ccgggccgat
     5941 gatgctaacg tggttcgtga ccgtgacctt gaggtggaca ccaccctcaa gagcctgagt
     6001 cagcagattg agaacatccg cagccccgaa ggcagccgca agaaccctgc ccgcacatgc
     6061 cgcgacctca agatgtgcca ctctgactgg aagagcggag agtactggat cgaccctaac
     6121 caaggctgca acctggacgc catcaaggtc tactgcaaca tggagacagg tcagacctgt
     6181 gtgttcccta ctcagccgtc tgtgcctcag aagaactggt acatcagccc gaaccccaag
     6241 gaaaagaagc acgtctggtt tggagagagc atgaccgatg gattcccgtt cgagtacgga
     6301 agcgagggct ccgaccccgc cgatgtcgct atccagctga ccttcctgcg cctaatgtcc
     6361 accgaggcct cccagaacat cacctatcac tgcaagaaca gcgtagccta catggaccag
     6421 cagactggca acctcaagaa ggccctgctc ctccagggat ccaacgagat cgagctcaga
     6481 ggcgaaggca acagtcgctt cacctacagc acccttgtgg acggctgcac gagtcacacc
     6541 ggaacttggg gcaagacagt catcgaatac aaaaccacca agacctcccg cctgcccatc
     6601 atcgatgtgg ctcccttgga cattggtgcc ccagaccagg aattcggact agacattggc
     6661 cctgcctgct tcgtgggatc cgactacaaa gaccatgacg gtgattataa agatcatgac
     6721 atcgactaca aggatgacga tgacaagtag cggccgcgaa ggatctgcga tcgctccggt
     6781 gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc
     6841 ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg
     6901 tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc
     6961 gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac agctgaagct tcgaggggct
     7021 cgcatctctc cttcacgcgc ccgccgccct acctgaggcc gccatccacg ccggttgagt
     7081 cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg cgtccgccgt ctaggtaagt
     7141 ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc cttggagcct acctagactc
     7201 agccggctct ccacgctttg cctgaccctg cttgctcaac tctacgtctt tgtttcgttt
     7261 tctgttctgc gccgttacag atccaagctg tgaccggcgc ctacgctaga cgccaccatg
     7321 gagagcgacg agagcggcct gcccgccatg gagatcgagt gccgcatcac cggcaccctg
     7381 aacggcgtgg agttcgagct ggtgggcggc ggagagggca cccccaagca gggccgcatg
     7441 accaacaaga tgaagagcac caaaggcgcc ctgaccttca gcccctacct gctgagccac
     7501 gtgatgggct acggcttcta ccacttcggc acctacccca gcggctacga gaaccccttc
     7561 ctgcacgcca tcaacaacgg cggctacacc aacacccgca tcgagaagta cgaggacggc
     7621 ggcgtgctgc acgtgagctt cagctaccgc tacgaggccg gccgcgtgat cggcgacttc
     7681 aaggtggtgg gcaccggctt ccccgaggac agcgtgatct tcaccgacaa gatcatccgc
     7741 agcaacgcca ccgtggagca cctgcacccc atgggcgata acgtgctggt gggcagcttc
     7801 gcccgcacct tcagcctgcg cgacggcggc tactacagct tcgtggtgga cagccacatg
     7861 cacttcaaga gcgccatcca ccccagcatc ctgcagaacg ggggccccat gttcgccttc
     7921 cgccgcgtgg aggagctgca cagcaacacc gagctgggca tcgtggagta ccagcacgcc
     7981 ttcaagaccc ccatcgcctt cgccagatcc cgcgctcagt cgtccaattc tgccgtggac
     8041 ggcaccgccg gacccggctc caccggatct cgcgagggca gaggaagtct tctaacatgc
     8101 ggtgacgtgg aggagaatcc cggccctatg accgagtaca agcccacggt gcgcctcgcc
     8161 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc
     8221 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa
     8281 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc
     8341 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc
     8401 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc
     8461 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg
     8521 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc
     8581 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac
     8641 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg
     8701 tgcatgaccc gcaagcccgg tgcctgaaat caacctctgg attacaaaat ttgtgaaaga
     8761 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     8821 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     8881 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     8941 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     9001 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     9061 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     9121 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     9181 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     9241 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     9301 tgggccgcct ccccgcctgg tacctttaag accaatgact tacaaggcag ctgtagatct
     9361 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagata
     9421 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct
     9481 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     9541 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     9601 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata
     9661 acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg
     9721 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     9781 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg
     9841 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     9901 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     9961 cagaagtagt gaggaggctt ttttggaggc ctagactttt gcagagacca aattcgtaat
    10021 catgtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
    10081 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
    10141 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
    10201 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
    10261 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
    10321 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
    10381 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
    10441 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
    10501 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
    10561 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
    10621 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
    10681 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
    10741 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
    10801 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
    10861 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
    10921 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
    10981 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
    11041 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
    11101 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
    11161 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
    11221 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
    11281 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
    11341 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
    11401 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
    11461 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
    11521 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
    11581 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
    11641 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
    11701 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
    11761 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
    11821 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
    11881 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
    11941 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
    12001 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
    12061 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
    12121 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
    12181 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
    12241 tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
    12301 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
    12361 gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag
    12421 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
    12481 gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
    12541 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
    12601 agggttttcc cagtcacgac gttgtaaaac gacggccagt gccaagctg
//
