LOCUS V046467 9371 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V046467 VERSION V046467 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1804..1920 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2010..2213 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2302..4203 /label="Clpx(NM_011802)" /note="Clpx(NM_011802)" /gene="Clpx" CDS 4210..4236 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 4240..4266 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 4270..4296 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" promoter 4341..4552 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 4565..4833 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" CDS 4860..5459 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 5466..6054 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 6128..6361 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 6433..6554 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 6573..6708 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(6746..6762) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 6770..6786 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(6794..6824) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 6839..6860 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(7148..7736) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(7907..8767) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(8768..8872) /gene="bla" /label="AmpR promoter" primer_bind 9346..9362 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag 2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct 2281 agagctagcg aattcgccac catgtccagt tgcggcgctt gtacctgcgg cgctgcggcc 2341 gcccggcttc tcaccacttc gctcacctcc gcgcagagag gtatttcgtg tgggcgaatc 2401 catgtgccag tgttaggaag actggggacg accttggacg ctcaggctct gcgcagagct 2461 cctcttagaa ccttttcaga aacaccagca tactttgcct caaaagacgg ggcaaacaag 2521 gatggctccg gagatggaaa taagaaatca gtgactgaag gaagcagtaa gaaatcaggc 2581 tctgggaatt ctgggaaagg tggaaaccag cttcgctgtc ctaaatgtgg tgatttgtgt 2641 acacacgtgg agacctttgt gtcttccacg cgttttgtta agtgtgaaaa atgtcatcat 2701 ttttttgtgg tgctgtcgga agcagactca aagaaaagca taattaagga acccgagtcc 2761 gctgcagagg ctgtgaagtt ggcattccag cagaagccac ctccgccccc caaaaagatt 2821 tataactacc tcgacaagta tgttgttggc cagtcgtttg ctaagaaagt gctttcagtt 2881 gccgtgtata atcattataa gagaatatat aataatattc cagctaatct gagacagcaa 2941 gcagaggctg agaagcagac atcactaaca cctagagagt tagaaataag aagacgggag 3001 gatgagtaca gatttacaaa gttgcttcag atcgctggga tcagcccaca cggcaatgcc 3061 ttgggagcct ctatgcagca gcaggtaaac cagcaaatgc ctcaggagaa aagaggaggt 3121 gaagtgttgg actcttctca agatgatata aaactggaaa aaagtaatat tttgctgctt 3181 ggaccaactg ggtcaggtaa aacccttctg gcacaaactt tagctaaatg ccttgatgtt 3241 ccttttgcta tctgtgactg tacaactttg acccaggctg gatatgtagg tgaagatatt 3301 gaatctgtga ttgccaaact actccaagat gccaattaca atgtagagaa agcacaacaa 3361 ggaattgtct ttctggatga agtagataag attggcagtg tgccaggcat tcatcaatta 3421 cgggatgtag gtggagaagg cgttcagcaa ggtttattaa aacttctaga aggcacaata 3481 gtcaatgttc cagaaaagaa ttctcgaaaa ctacgtggag agacagttca agttgataca 3541 acaaatgtct tgtttgtggc atctggtgcc tttaatggct tagacagaat catcagcagg 3601 aggaaaaatg aaaagtatct cggctttgga acaccatcta acctgggaaa gggcagaagg 3661 gctgcagctg cagcggatct tgctaaccga agtggggaat ctaacactca tcaggacatt 3721 gaggagaaag accggttact ccgccatgtg gaagccagag atcttattga gtttggcatg 3781 ataccagagt ttgtgggacg gttgcctgtg gtggttcctt tgcacagcct agatgagaag 3841 acacttgtac aaatactgac cgagccacgg aatgctgtta ttcctcagta tcaggccttg 3901 ttcagcatgg ataagtgtga gctgaatgtt actgaggatg ctctgaaagc catagcgaga 3961 ttggccctgg aaagaaaaac aggtgcacga ggccttcggt ctataatgga aaagctgtta 4021 ctagaaccaa tgttcgaagt tcctaattct gacattgtat gtgtggaagt tgacaaagaa 4081 gtagtagaag gcaaaaagga accaggatac attcgggcac cctcaaaaga gtcctctgaa 4141 gaggaatatg actctggagt ggaggaggat ggatggcctc gtcaagcgga tgctgcaaac 4201 agcggatcct acccatacga cgtacctgat tatgcaactt atccttatga tgtacctgat 4261 tatgctagct atccttatga tgtcccagac tacgcatagg cggccgcgaa ggatctgcga 4321 tcgctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg 4381 gggaggggtc ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa actgggaaag 4441 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc 4501 agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac agctgaagct 4561 tcgaggggct cgcatctctc cttcacgcgc ccgccgccct acctgaggcc gccatccacg 4621 ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg cgtccgccgt 4681 ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc cttggagcct 4741 acctagactc agccggctct ccacgctttg cctgaccctg cttgctcaac tctacgtctt 4801 tgtttcgttt tctgttctgc gccgttacag atccaagctg tgaccggcgc ctacgctaga 4861 tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc cgggccgtac 4921 gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gacccggacc 4981 gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca 5041 tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga 5101 gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt 5161 cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc 5221 ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca 5281 gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg 5341 agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg 5401 acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgag 5461 tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg 5521 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt 5581 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg 5641 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc 5701 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc 5761 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc 5821 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc 5881 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg 5941 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc 6001 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggta 6061 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag 6121 gggggactgg aagggctaat tcactcccaa cgaaaataag atctgctttt tgcttgtact 6181 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca 6241 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg 6301 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc 6361 agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca 6421 gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc 6481 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc 6541 atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg cccagttccg 6601 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct 6661 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta gacttttgca 6721 gagacggccc aaattcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc 6781 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat 6841 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc 6901 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg 6961 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag 7021 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag 7081 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc 7141 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc 7201 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc 7261 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt 7321 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg 7381 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat 7441 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag 7501 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt 7561 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc 7621 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta 7681 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag 7741 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga 7801 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa 7861 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa 7921 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc 7981 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga 8041 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa 8101 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt 8161 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg 8221 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc 8281 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg 8341 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag 8401 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt 8461 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt 8521 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac 8581 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac 8641 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag 8701 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa 8761 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga 8821 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc 8881 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa 8941 ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct 9001 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac 9061 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg 9121 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg 9181 taaggagaaa ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag 9241 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa 9301 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca 9361 gtgccaagct g //