LOCUS       V043196                10942 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V043196
VERSION     V043196
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4968
                     /label="Chsy3(NM_001081328)"
                     /note="Chsy3(NM_001081328)"
                     /gene="Chsy3"
     CDS             4975..5040
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5084..5295
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5308..5576
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5605..5614
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5635..6297
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6367..6420
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6421..7020
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7021..7609
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7683..7916
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7988..8109
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8149..8284
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8317..8333)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8341..8357
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8365..8395)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8410..8431
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8719..9307)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9478..10338)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10339..10443)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10917..10933
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg ccgtgcgctc ccgccgccca
     2341 tgggtgagcg tggcattggg gttggtcctg ggcttcaccg ccgcgtcctg gctcatcgcc
     2401 ccccgggtgg ccgagctgag cgagaagagg cgacgcggct ccagtctttg ctcctactac
     2461 ggccgctcgg ctaccgggcc ccgcgcggac gcgcagcagc tgctccccca accccagtcc
     2521 cggccgcggc tagagcagtc gccgccccct gccagccacg agctccccgg tcctcagcag
     2581 ccggaggcgg cgcccggagg tcccagtttt cggagcagcc cctggcagca gccggctctg
     2641 ttgccgcaga ggaggcgagg acacacgccc gaaggtgcga cggcgcttcc cggcgctccg
     2701 gctgccaaag gggaaccaga ggaggaggat gggggcgcgg ctgaccctcg gaagggtggc
     2761 cggccgggga gcagccacaa cggcagcggg gacgggggtg ccgctgtccc gacctccgga
     2821 cccggggact tcctgtacgt gggtgtgatg accgcacaga agtacctggg cagtcgcgcg
     2881 ctggccgcgc agcggacctg ggcgcgcttc atccctggcc gcgtggagtt cttttccagt
     2941 cagcaatctc ccagtgctgc gcttggccag cccccgccac ctttgcctgt catcgcgctg
     3001 ccaggggtcg acgattccta ccctccccag aaaaagtcct tcatgatgat caagtacatg
     3061 cacgaccact atctggacaa gtatgagtgg ttcatgcgcg ccgacgacga tgtctacatc
     3121 aaaggtgata agttagaaga atttctaaga tccctaaata gcagcaagcc tctctacctg
     3181 ggacagacgg gcctgggcaa tactgaagaa cttggaaagc tggggctgga gcccggggag
     3241 aacttctgca tgggaggacc tggcatgatc ttcagcagag aggttctcag gcggatggtg
     3301 cctcatatcg gcgaatgcct ccgagagatg tacaccacac acgaagacgt agaagtagga
     3361 aggtgtgttc gccgtttcgg cgggacgcag tgtgtctggt cttatgagat gcagcagctg
     3421 ttccatgaaa actacgaaca caatcgcaag ggttacatcc aagacctcca caacagcaaa
     3481 atccacgcag ccatcacgct ccatccgaac aaaaggcccg cgtaccagta cagacttcat
     3541 aactacatgc tcagccgcaa gatctccgag ctccgctacc gcaccatcca gctccaccgg
     3601 gagagcgctc tcatgagcaa gctcagcaac agtgaagtga gcaaagagga ccaacagctg
     3661 ggaaggacgc cgtccttcaa ccacttccag cctcgggaga gaaatgaagt catggagtgg
     3721 gagttcctga cggggaagct gctttactca gctgcagaga accagcctcc tcgacagagc
     3781 atcaacagca tcctaaggtc agctctggat gacactgtcc tgcaggtgat ggagatgatc
     3841 aacgagaatg ccaagagtag gggccgactc atcgacttca aggaaattca gtatggctac
     3901 cgcagggttg atcccatgca cggggttgag tacatattgg acctgctact cctgtacaaa
     3961 aggcacaaag gaaggaaact gactgtgcct gtgaggcgcc atgcctatct tcagcagcca
     4021 tttagcaagc ctttcttcag agaggtggaa gaactcgacg tcaaccgtct ggtggagagt
     4081 atcaacagcg gtacacagtc attctccgtt atatccaatt ctctaaaaat tctctcttct
     4141 cttcaagagg ccaaagacat aggagggcac aatgaaaaga aagtacacat tctcgttcca
     4201 ctcgttggaa ggtacgacat tttcttgaga ttcatggaaa attttgaaag tacgtgtctt
     4261 atcccaaagc aaaatgtcaa gcttgtcatc atccttttca gcagggatgc tggccaagag
     4321 tccatcaagc acattgagct gatacaagaa tatcagagca ggtatcccag tgcagaaatg
     4381 atgctcattc ccatgaaggg agagttttcc agaggtcttg gtcttgaaat ggcttcttcc
     4441 cagtttgaca atgacacatt gctgctattt tgtgatgttg acttgatttt cagaggagac
     4501 ttcctccaac gctgtcgaga caatacagtt cagggacaac aggtatatta ccccatcatc
     4561 tttagccagt atgacccaaa ggtcacccat atgagaaatc ctcccacaga gggtgacttt
     4621 gtattctcaa aggaaactgg gttttggaga gactatggct acggaatcac atgcatttac
     4681 aaaagcgatc tactgggtgc aggtggattt gatacctcaa tacaaggctg gggactggaa
     4741 gatgtagatc tctataataa agtcatccta tctggcttac ggcccttcag aagtcaagaa
     4801 gtgggagtgg tgcatatttt ccatcctgtt cattgtgatc ctaacttgga ccctaagcag
     4861 tataagatgt gcttaggatc caaagcaagt acttttgcct caaccatgca actggctgaa
     4921 ctctggttag aaaaacattt gggtgtcagg gataatcgaa ctctctccgg atccgactac
     4981 aaagaccatg acggtgatta taaagatcat gacatcgact acaaggatga cgatgacaag
     5041 tagcggccgc gaaggatctg cgatcgctcc ggtgcccgtc agtgggcaga gcgcacatcg
     5101 cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaacgggtgc ctagagaagg
     5161 tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt
     5221 gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt
     5281 gccgccagaa cacagctgaa gcttcgaggg gctcgcatct ctccttcacg cgcccgccgc
     5341 cctacctgag gccgccatcc acgccggttg agtcgcgttc tgccgcctcc cgcctgtggt
     5401 gcctcctgaa ctgcgtccgc cgtctaggta agtttaaagc tcaggtcgag accgggcctt
     5461 tgtccggcgc tcccttggag cctacctaga ctcagccggc tctccacgct ttgcctgacc
     5521 ctgcttgctc aactctacgt ctttgtttcg ttttctgttc tgcgccgtta cagatccaag
     5581 ctgtgaccgg cgcctacgct agacgccacc atggagagcg acgagagcgg cctgcccgcc
     5641 atggagatcg agtgccgcat caccggcacc ctgaacggcg tggagttcga gctggtgggc
     5701 ggcggagagg gcacccccaa gcagggccgc atgaccaaca agatgaagag caccaaaggc
     5761 gccctgacct tcagccccta cctgctgagc cacgtgatgg gctacggctt ctaccacttc
     5821 ggcacctacc ccagcggcta cgagaacccc ttcctgcacg ccatcaacaa cggcggctac
     5881 accaacaccc gcatcgagaa gtacgaggac ggcggcgtgc tgcacgtgag cttcagctac
     5941 cgctacgagg ccggccgcgt gatcggcgac ttcaaggtgg tgggcaccgg cttccccgag
     6001 gacagcgtga tcttcaccga caagatcatc cgcagcaacg ccaccgtgga gcacctgcac
     6061 cccatgggcg ataacgtgct ggtgggcagc ttcgcccgca ccttcagcct gcgcgacggc
     6121 ggctactaca gcttcgtggt ggacagccac atgcacttca agagcgccat ccaccccagc
     6181 atcctgcaga acgggggccc catgttcgcc ttccgccgcg tggaggagct gcacagcaac
     6241 accgagctgg gcatcgtgga gtaccagcac gccttcaaga cccccatcgc cttcgccaga
     6301 tcccgcgctc agtcgtccaa ttctgccgtg gacggcaccg ccggacccgg ctccaccgga
     6361 tctcgcgagg gcagaggaag tcttctaaca tgcggtgacg tggaggagaa tcccggccct
     6421 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta
     6481 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac
     6541 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac
     6601 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag
     6661 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt
     6721 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag
     6781 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc
     6841 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg
     6901 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc
     6961 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga
     7021 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     7081 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     7141 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     7201 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     7261 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     7321 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     7381 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
     7441 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     7501 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     7561 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggtaccttt
     7621 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     7681 actggaaggg ctaattcact cccaacgaag ataagatctg ctttttgctt gtactgggtc
     7741 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     7801 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     7861 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag
     7921 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag
     7981 tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     8041 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     8101 tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta
     8161 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     8221 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     8281 ggcctagact tttgcagaga ccaaattcgt aatcatgtca tagctgtttc ctgtgtgaaa
     8341 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     8401 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     8461 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     8521 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     8581 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     8641 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     8701 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     8761 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     8821 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     8881 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     8941 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     9001 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     9061 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     9121 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     9181 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     9241 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     9301 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     9361 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     9421 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     9481 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     9541 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     9601 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     9661 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     9721 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     9781 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     9841 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     9901 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     9961 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
    10021 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
    10081 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
    10141 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
    10201 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
    10261 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
    10321 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
    10381 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
    10441 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
    10501 aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg
    10561 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
    10621 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct
    10681 taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc
    10741 gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca ggctgcgcaa
    10801 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg
    10861 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa
    10921 aacgacggcc agtgccaagc tg
//
