LOCUS       V027275                12046 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V027275
VERSION     V027275
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..6072
                     /label="Cfh(NM_009888)"
                     /note="Cfh(NM_009888)"
                     /gene="Cfh"
     CDS             6079..6144
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        6188..6399
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6412..6680
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      6709..6718
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6739..7401
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             7471..7524
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             7525..8124
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    8125..8713
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8787..9020
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    9092..9213
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      9253..9388
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(9421..9437)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9445..9461
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9469..9499)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9514..9535
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9823..10411)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10582..11442)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11443..11547)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     12021..12037
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg tacagcacag atttctcttg
     2341 gagtcagttg gtcccagaaa gatccaaatt atgagactgt cagcaagaat tatttggctt
     2401 atattatgga ctgtttgtgc agcagaagat tgtaaaggtc ctcctccaag agaaaattca
     2461 gaaattctct caggctcgtg gtcagaacaa ctatatccag aaggcaccca ggctacctac
     2521 aaatgccgcc ctggataccg aacacttggc actattgtaa aagtatgcaa gaatggaaaa
     2581 tgggtggcgt ctaacccatc caggatatgt cggaaaaagc cttgtgggca tcccggagac
     2641 acaccctttg ggtcctttag gctggcagtt ggatctcaat ttgagtttgg tgcaaaggtt
     2701 gtttatacct gtgatgatgg gtatcaacta ttaggtgaaa ttgattaccg tgaatgtggt
     2761 gcagatgggt ggatcaatga tattccacta tgtgaagttg tgaagtgtct acctgtgaca
     2821 gaactcgaga atggaagaat tgtgagtggt gcagcagaaa cagaccagga atactatttt
     2881 ggacaggtgg tgcggtttga atgcaattca ggcttcaaga ttgaaggaca taaggaaatt
     2941 cattgctcag aaaatggcct ttggagcaat gaaaagccac gatgtgtgga aattctctgc
     3001 acaccaccgc gagtggaaaa tggagatggt ataaatgtga aaccagttta caaggagaat
     3061 gaaagatacc actataagtg taagcatggt tatgtgccca aagaaagagg ggatgccgtc
     3121 tgcacaggct ctggatggag ttctcagcct ttctgtgaag aaaagagatg ctcacctcct
     3181 tatattctaa atggtatcta cacacctcac aggattatac acagaagtga tgatgaaatc
     3241 agatatgaat gtaattatgg cttctatcct gtaactggat caactgtttc aaagtgtaca
     3301 cccactggct ggatccctgt tccaagatgt accttgaaac catgtgaatt tccacaattc
     3361 aaatatggac gtctgtatta tgaagagagc ctgagaccca acttcccagt atctatagga
     3421 aataagtaca gctataagtg tgacaacggg ttttcaccac cttctgggta ttcctgggac
     3481 taccttcgtt gcacagcaca agggtgggag cctgaagtcc catgcgtcag gaaatgtgtt
     3541 ttccattatg tggagaatgg agactctgca tactgggaaa aagtatatgt gcagggtcag
     3601 tctttaaaag tccagtgtta caatggctat agtcttcaaa atggtcaaga cacaatgaca
     3661 tgtacagaga atggctggtc ccctcctccc aaatgcatcc gtatcaagac atgttcagca
     3721 tcagatatac acattgacaa tggatttctt tctgaatctt cttctatata tgctctaaat
     3781 agagaaacat cctatagatg taagcaggga tatgtgacaa atactggaga aatatcagga
     3841 tcaataactt gccttcaaaa tggatggtca cctcaaccct catgcattaa gtcttgtgat
     3901 atgcctgtat ttgagaattc tataactaag aatactagga catggtttaa gctcaatgac
     3961 aaattagact atgaatgtct cgttggattt gaaaatgaat ataaacatac caaaggctct
     4021 ataacatgta cttattatgg atggtctgat acaccctcat gttatgaaag agaatgcagt
     4081 gttcccactc tagaccgaaa actagtcgtt tcccccagaa aagaaaaata cagagttgga
     4141 gatttgttgg aattctcctg ccattcagga cacagagttg ggccagattc agtgcaatgc
     4201 taccactttg gatggtctcc tggtttccct acatgtaaag gtcaagtagc atcatgtgca
     4261 ccacctcttg aaattcttaa tggggaaatt aatggagcaa aaaaagttga atacagccat
     4321 ggtgaagtgg tgaaatatga ttgcaaacct agattcctac tgaagggacc caataaaatc
     4381 cagtgtgttg atgggaattg gacaaccttg cctgtatgta ttgaggagga gagaacatgt
     4441 ggagacattc ctgaacttga acatggctct gccaagtgtt ctgttcctcc ctaccaccat
     4501 ggagattcag tggagttcat ttgtgaagaa aacttcacaa tgattggaca tgggtcagtt
     4561 tcttgcatta gtggaaaatg gacccagctt cctaaatgtg ttgcaacaga ccaactggag
     4621 aagtgtagag tgctgaagtc aactggcata gaagcaataa aaccaaaatt gactgaattt
     4681 acgcataact ccaccatgga ttacaaatgt agagacaagc aggagtacga acgctcaatc
     4741 tgtatcaatg gaaaatggga tcctgaacca aactgtacaa gcaaaacatc ctgccctcct
     4801 ccaccgcaga ttccaaatac ccaagtgatt gaaaccaccg tgaaatactt ggatggagaa
     4861 aaattatctg ttctttgcca agacaattac ctaactcagg actcagaaga aatggtgtgc
     4921 aaagatggaa ggtggcagtc attacctcgc tgcattgaaa aaattccatg ttcccagccc
     4981 cctacaatag aacatggatc tattaattta cccagatctt cagaagaaag gagagattcc
     5041 attgagtcca gcagtcatga acatggaact acattcagct atgtctgtga tgatggtttc
     5101 aggatacctg aagaaaatag gataacctgc tacatgggaa aatggagcac tccacctcgc
     5161 tgtgttggac ttccttgtgg acctccacct tcaattcctc ttggtactgt ttctcttgag
     5221 ctagagagtt accaacatgg ggaagaggtt acataccatt gttctacagg ctttggaatt
     5281 gatggaccag catttattat atgcgaagga ggaaagtggt ctgacccacc aaaatgcata
     5341 aaaacggatt gtgacgtttt acccacagtt aaaaatgcca taataagagg aaagagcaaa
     5401 aaatcatata ggacaggaga acaagtgaca ttcagatgtc aatctcctta tcaaatgaat
     5461 ggctcagaca ctgtgacatg tgttaatagt cggtggattg gacagccagt atgcaaagat
     5521 aattcctgtg tggatccacc acatgtgcca aatgctacta tagtaacaag gaccaagaat
     5581 aaatatctac atggtgacag agtacgttat gaatgtaata aacctttgga actatttggg
     5641 caagtggaag tgatgtgtga aaatgggata tggacagaaa aaccaaagtg ccgagactca
     5701 acagggaaat gtgggcctcc tccacctatt gacaatggag acatcacctc cttgtcatta
     5761 ccagtatatg aaccattatc atcagttgaa tatcaatgcc agaagtatta tctccttaag
     5821 ggaaagaaga caataacatg tagaaatgga aagtggtctg agccaccaac atgcttacat
     5881 gcatgtgtaa taccagaaaa cattatggaa tcacacaata taattctcaa atggagacac
     5941 actgaaaaga tttattccca ttcaggggag gatattgaat ttggatgtaa atatggatat
     6001 tataaagcaa gagattcacc gccatttcgt acaaagtgca ttaatggcac catcaattat
     6061 cccacttgtg taggatccga ctacaaagac catgacggtg attataaaga tcatgacatc
     6121 gactacaagg atgacgatga caagtagcgg ccgcgaagga tctgcgatcg ctccggtgcc
     6181 cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc
     6241 aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac
     6301 tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg
     6361 aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg aggggctcgc
     6421 atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg gttgagtcgc
     6481 gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta ggtaagttta
     6541 aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc tagactcagc
     6601 cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt ttcgttttct
     6661 gttctgcgcc gttacagatc caagctgtga ccggcgccta cgctagacgc caccatggag
     6721 agcgacgaga gcggcctgcc cgccatggag atcgagtgcc gcatcaccgg caccctgaac
     6781 ggcgtggagt tcgagctggt gggcggcgga gagggcaccc ccaagcaggg ccgcatgacc
     6841 aacaagatga agagcaccaa aggcgccctg accttcagcc cctacctgct gagccacgtg
     6901 atgggctacg gcttctacca cttcggcacc taccccagcg gctacgagaa ccccttcctg
     6961 cacgccatca acaacggcgg ctacaccaac acccgcatcg agaagtacga ggacggcggc
     7021 gtgctgcacg tgagcttcag ctaccgctac gaggccggcc gcgtgatcgg cgacttcaag
     7081 gtggtgggca ccggcttccc cgaggacagc gtgatcttca ccgacaagat catccgcagc
     7141 aacgccaccg tggagcacct gcaccccatg ggcgataacg tgctggtggg cagcttcgcc
     7201 cgcaccttca gcctgcgcga cggcggctac tacagcttcg tggtggacag ccacatgcac
     7261 ttcaagagcg ccatccaccc cagcatcctg cagaacgggg gccccatgtt cgccttccgc
     7321 cgcgtggagg agctgcacag caacaccgag ctgggcatcg tggagtacca gcacgccttc
     7381 aagaccccca tcgccttcgc cagatcccgc gctcagtcgt ccaattctgc cgtggacggc
     7441 accgccggac ccggctccac cggatctcgc gagggcagag gaagtcttct aacatgcggt
     7501 gacgtggagg agaatcccgg ccctatgacc gagtacaagc ccacggtgcg cctcgccacc
     7561 cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc
     7621 acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc
     7681 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg
     7741 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc
     7801 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc
     7861 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc
     7921 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag
     7981 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag
     8041 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc
     8101 atgacccgca agcccggtgc ctgaaatcaa cctctggatt acaaaatttg tgaaagattg
     8161 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     8221 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     8281 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     8341 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     8401 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     8461 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     8521 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     8581 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     8641 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     8701 gccgcctccc cgcctggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
     8761 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagataaga
     8821 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     8881 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     8941 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     9001 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact
     9061 tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta
     9121 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     9181 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc
     9241 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     9301 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     9361 aagtagtgag gaggcttttt tggaggccta gacttttgca gagaccaaat tcgtaatcat
     9421 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     9481 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     9541 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     9601 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     9661 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     9721 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     9781 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     9841 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     9901 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     9961 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
    10021 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
    10081 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
    10141 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
    10201 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
    10261 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
    10321 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
    10381 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
    10441 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
    10501 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
    10561 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
    10621 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
    10681 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
    10741 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
    10801 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
    10861 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
    10921 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
    10981 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
    11041 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
    11101 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
    11161 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
    11221 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
    11281 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
    11341 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
    11401 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
    11461 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
    11521 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
    11581 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
    11641 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
    11701 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
    11761 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt
    11821 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
    11881 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
    11941 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
    12001 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctg
//
