LOCUS       V039888                 9676 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V039888
VERSION     V039888
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..4578
                     /label="CUL4A(NM_001008895)"
                     /note="CUL4A(NM_001008895)"
                     /gene="CUL4A"
     CDS             4585..4602
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /transl_table=1
                     /translation="HHHHHH"
                     /label="6xHis"
     promoter        4646..4857
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4870..5138
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5165..5764
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    5771..6359
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6433..6666
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    6738..6859
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      6878..7013
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7051..7067)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7075..7091
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7099..7129)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7144..7165
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7453..8041)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8212..9072)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9073..9177)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     9651..9667
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catggcggac gaggccccgc ggaagggcag cttctcggcg
     2341 ctcgtgggcc gcaccaacgg cctcaccaag cccgcggccc tggccgccgc gcccgccaag
     2401 ccggggggcg cgggcggctc caagaagctg gtcatcaaga acttccgaga cagacctcgg
     2461 ctgcccgaca actacacgca ggacacgtgg cggaagctgc acgaggcggt gcgggccgtg
     2521 cagagcagca cctccatcag gtacaacctc gaggagctct accaggctgt ggaaaatctc
     2581 tgttctcaca aagtctcccc aatgctctac aagcaactgc gtcaggcctg tgaagaccac
     2641 gtccaggcac agatccttcc gtttagagaa gactcactag atagtgtttt atttttaaag
     2701 aagattaaca cgtgctggca ggaccactgc agacaaatga tcatgatcag aagcatcttc
     2761 ctgttcttgg accgcaccta tgtgctgcag aactccacgc tgccctccat ctgggatatg
     2821 ggattagaac tgtttagaac ccatattatt agtgataaaa tggttcagag taaaaccatt
     2881 gatggaatcc tactgctgat cgagcgcgag aggagcggcg aggccgtgga ccggagcctg
     2941 ttgcggagcc tcctgggcat gctgtctgac ctgcaggtgt ataaagattc atttgaactg
     3001 aaatttttgg aagagactaa ttgcttatat gctgccgaag gccaaaggtt aatgcaggaa
     3061 agagaggttc cagaatatct taaccatgta agtaaacgct tagaggaaga gggagacaga
     3121 gtaatcactt acttggacca cagcacacag aaaccactga ttgcttgtgt ggagaaacag
     3181 ctattaggag aacatttaac agcaattctg cagaaagggc tcgaccactt actggatgag
     3241 aacagagtgc cggacctcgc acagatgtac cagctgttca gccgggtgag gggcgggcag
     3301 caggcgctgc tgcagcactg gagcgagtac atcaagactt ttggaacagc gatcgtaatc
     3361 aatcctgaga aagacaaaga catggtccaa gacctgttgg acttcaagga caaggtggac
     3421 cacgtgatcg aggtctgctt ccagaagaat gagcggttcg tcaacctgat gaaggagtcc
     3481 tttgagacgt tcatcaacaa gagacccaac aagcctgcag aactgatcgc aaagcatgtg
     3541 gattcaaagt taagagcagg caacaaagaa gccacagacg aggagctgga gcggacgttg
     3601 gacaagatca tgatcctgtt caggtttatc cacggtaaag atgtctttga agcattttat
     3661 aaaaaagatt tggcaaaaag actccttgtt gggaaaagtg cctcagtcga tgctgaaaag
     3721 tctatgttgt caaagctcaa gcatgagtgc ggtgcagcct tcaccagcaa gctggaaggc
     3781 atgttcaagg acatggagct ttcgaaggac atcatggttc atttcaagca gcatatgcag
     3841 aatcagagtg actcaggccc tatagacctc acagtgaaca tactcacaat gggctactgg
     3901 ccaacataca cgcccatgga agtgcactta accccagaaa tgattaaact tcaggaagta
     3961 tttaaggcat tttatcttgg aaagcacagt ggtcgaaaac ttcagtggca aactactttg
     4021 ggacatgctg ttttaaaagc ggagtttaaa gaagggaaga aggaattcca ggtgtccctc
     4081 ttccagacac tggtgctcct catgttcaac gagggagatg gcttcagctt tgaggagata
     4141 aaaatggcca cggggataga ggatagtgaa ttgcgcagaa cgctgcagtc cctggcctgt
     4201 ggcaaagcac gtgtgctgat taaaagtccc aaaggaaagg aagtggaaga tggagacaag
     4261 ttcattttta atggagagtt caagcacaag ttgtttagaa taaagatcaa tcaaattcag
     4321 atgaaggaaa ctgttgagga acaggttagc accactgaga gagtgtttca ggatagacaa
     4381 tatcagattg atgctgctat cgtcagaata atgaagatga gaaagactct tggtcataat
     4441 cttctagttt ctgaattata taatcagctg aaatttccag taaagcctgg agatttgaaa
     4501 aagagaattg aatctctgat agacagagac tatatggaga gagacaaaga caatccgaat
     4561 cagtaccact acgtggccgg atcccatcac catcaccatc actagcggcc gcgaaggatc
     4621 tgcgatcgct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag
     4681 ttggggggag gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg ggtaaactgg
     4741 gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata
     4801 agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacagctg
     4861 aagcttcgag gggctcgcat ctctccttca cgcgcccgcc gccctacctg aggccgccat
     4921 ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg aactgcgtcc
     4981 gccgtctagg taagtttaaa gctcaggtcg agaccgggcc tttgtccggc gctcccttgg
     5041 agcctaccta gactcagccg gctctccacg ctttgcctga ccctgcttgc tcaactctac
     5101 gtctttgttt cgttttctgt tctgcgccgt tacagatcca agctgtgacc ggcgcctacg
     5161 ctagatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tcccccgggc
     5221 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgaccc
     5281 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
     5341 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc
     5401 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag
     5461 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
     5521 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
     5581 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt
     5641 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
     5701 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
     5761 ctgagtcgac aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     5821 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     5881 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     5941 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     6001 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     6061 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     6121 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc
     6181 ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     6241 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     6301 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc
     6361 tggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     6421 aaaagggggg actggaaggg ctaattcact cccaacgaaa ataagatctg ctttttgctt
     6481 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     6541 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     6601 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     6661 ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     6721 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     6781 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     6841 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccag
     6901 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     6961 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagactt
     7021 ttgcagagac ggcccaaatt cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     7081 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     7141 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     7201 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     7261 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     7321 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     7381 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     7441 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     7501 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     7561 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     7621 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     7681 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     7741 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     7801 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     7861 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     7921 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     7981 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     8041 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     8101 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     8161 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     8221 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     8281 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     8341 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     8401 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     8461 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     8521 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     8581 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     8641 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     8701 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     8761 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     8821 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     8881 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     8941 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     9001 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     9061 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     9121 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     9181 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
     9241 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa
     9301 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
     9361 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta
     9421 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
     9481 atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg
     9541 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
     9601 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
     9661 ggccagtgcc aagctg
//
