LOCUS       V038263                10567 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V038263
VERSION     V038263
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4593
                     /label="CUL4A(NM_001008895)"
                     /note="CUL4A(NM_001008895)"
                     /gene="CUL4A"
     CDS             4600..4665
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4709..4920
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4933..5201
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5230..5239
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5260..5922
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5992..6045
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6046..6645
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6646..7234
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7308..7541
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7613..7734
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7774..7909
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7942..7958)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7966..7982
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7990..8020)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8035..8056
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8344..8932)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9103..9963)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9964..10068)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10542..10558
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg cggacgaggc cccgcggaag
     2341 ggcagcttct cggcgctcgt gggccgcacc aacggcctca ccaagcccgc ggccctggcc
     2401 gccgcgcccg ccaagccggg gggcgcgggc ggctccaaga agctggtcat caagaacttc
     2461 cgagacagac ctcggctgcc cgacaactac acgcaggaca cgtggcggaa gctgcacgag
     2521 gcggtgcggg ccgtgcagag cagcacctcc atcaggtaca acctcgagga gctctaccag
     2581 gctgtggaaa atctctgttc tcacaaagtc tccccaatgc tctacaagca actgcgtcag
     2641 gcctgtgaag accacgtcca ggcacagatc cttccgttta gagaagactc actagatagt
     2701 gttttatttt taaagaagat taacacgtgc tggcaggacc actgcagaca aatgatcatg
     2761 atcagaagca tcttcctgtt cttggaccgc acctatgtgc tgcagaactc cacgctgccc
     2821 tccatctggg atatgggatt agaactgttt agaacccata ttattagtga taaaatggtt
     2881 cagagtaaaa ccattgatgg aatcctactg ctgatcgagc gcgagaggag cggcgaggcc
     2941 gtggaccgga gcctgttgcg gagcctcctg ggcatgctgt ctgacctgca ggtgtataaa
     3001 gattcatttg aactgaaatt tttggaagag actaattgct tatatgctgc cgaaggccaa
     3061 aggttaatgc aggaaagaga ggttccagaa tatcttaacc atgtaagtaa acgcttagag
     3121 gaagagggag acagagtaat cacttacttg gaccacagca cacagaaacc actgattgct
     3181 tgtgtggaga aacagctatt aggagaacat ttaacagcaa ttctgcagaa agggctcgac
     3241 cacttactgg atgagaacag agtgccggac ctcgcacaga tgtaccagct gttcagccgg
     3301 gtgaggggcg ggcagcaggc gctgctgcag cactggagcg agtacatcaa gacttttgga
     3361 acagcgatcg taatcaatcc tgagaaagac aaagacatgg tccaagacct gttggacttc
     3421 aaggacaagg tggaccacgt gatcgaggtc tgcttccaga agaatgagcg gttcgtcaac
     3481 ctgatgaagg agtcctttga gacgttcatc aacaagagac ccaacaagcc tgcagaactg
     3541 atcgcaaagc atgtggattc aaagttaaga gcaggcaaca aagaagccac agacgaggag
     3601 ctggagcgga cgttggacaa gatcatgatc ctgttcaggt ttatccacgg taaagatgtc
     3661 tttgaagcat tttataaaaa agatttggca aaaagactcc ttgttgggaa aagtgcctca
     3721 gtcgatgctg aaaagtctat gttgtcaaag ctcaagcatg agtgcggtgc agccttcacc
     3781 agcaagctgg aaggcatgtt caaggacatg gagctttcga aggacatcat ggttcatttc
     3841 aagcagcata tgcagaatca gagtgactca ggccctatag acctcacagt gaacatactc
     3901 acaatgggct actggccaac atacacgccc atggaagtgc acttaacccc agaaatgatt
     3961 aaacttcagg aagtatttaa ggcattttat cttggaaagc acagtggtcg aaaacttcag
     4021 tggcaaacta ctttgggaca tgctgtttta aaagcggagt ttaaagaagg gaagaaggaa
     4081 ttccaggtgt ccctcttcca gacactggtg ctcctcatgt tcaacgaggg agatggcttc
     4141 agctttgagg agataaaaat ggccacgggg atagaggata gtgaattgcg cagaacgctg
     4201 cagtccctgg cctgtggcaa agcacgtgtg ctgattaaaa gtcccaaagg aaaggaagtg
     4261 gaagatggag acaagttcat ttttaatgga gagttcaagc acaagttgtt tagaataaag
     4321 atcaatcaaa ttcagatgaa ggaaactgtt gaggaacagg ttagcaccac tgagagagtg
     4381 tttcaggata gacaatatca gattgatgct gctatcgtca gaataatgaa gatgagaaag
     4441 actcttggtc ataatcttct agtttctgaa ttatataatc agctgaaatt tccagtaaag
     4501 cctggagatt tgaaaaagag aattgaatct ctgatagaca gagactatat ggagagagac
     4561 aaagacaatc cgaatcagta ccactacgtg gccggatccg actacaaaga ccatgacggt
     4621 gattataaag atcatgacat cgactacaag gatgacgatg acaagtagcg gccgcgaagg
     4681 atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag
     4741 aagttggggg gaggggtcgg caattgaacg ggtgcctaga gaaggtggcg cggggtaaac
     4801 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat
     4861 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag
     4921 ctgaagcttc gaggggctcg catctctcct tcacgcgccc gccgccctac ctgaggccgc
     4981 catccacgcc ggttgagtcg cgttctgccg cctcccgcct gtggtgcctc ctgaactgcg
     5041 tccgccgtct aggtaagttt aaagctcagg tcgagaccgg gcctttgtcc ggcgctccct
     5101 tggagcctac ctagactcag ccggctctcc acgctttgcc tgaccctgct tgctcaactc
     5161 tacgtctttg tttcgttttc tgttctgcgc cgttacagat ccaagctgtg accggcgcct
     5221 acgctagacg ccaccatgga gagcgacgag agcggcctgc ccgccatgga gatcgagtgc
     5281 cgcatcaccg gcaccctgaa cggcgtggag ttcgagctgg tgggcggcgg agagggcacc
     5341 cccaagcagg gccgcatgac caacaagatg aagagcacca aaggcgccct gaccttcagc
     5401 ccctacctgc tgagccacgt gatgggctac ggcttctacc acttcggcac ctaccccagc
     5461 ggctacgaga accccttcct gcacgccatc aacaacggcg gctacaccaa cacccgcatc
     5521 gagaagtacg aggacggcgg cgtgctgcac gtgagcttca gctaccgcta cgaggccggc
     5581 cgcgtgatcg gcgacttcaa ggtggtgggc accggcttcc ccgaggacag cgtgatcttc
     5641 accgacaaga tcatccgcag caacgccacc gtggagcacc tgcaccccat gggcgataac
     5701 gtgctggtgg gcagcttcgc ccgcaccttc agcctgcgcg acggcggcta ctacagcttc
     5761 gtggtggaca gccacatgca cttcaagagc gccatccacc ccagcatcct gcagaacggg
     5821 ggccccatgt tcgccttccg ccgcgtggag gagctgcaca gcaacaccga gctgggcatc
     5881 gtggagtacc agcacgcctt caagaccccc atcgccttcg ccagatcccg cgctcagtcg
     5941 tccaattctg ccgtggacgg caccgccgga cccggctcca ccggatctcg cgagggcaga
     6001 ggaagtcttc taacatgcgg tgacgtggag gagaatcccg gccctatgac cgagtacaag
     6061 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc
     6121 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg
     6181 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
     6241 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg
     6301 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg
     6361 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg
     6421 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
     6481 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc
     6541 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
     6601 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgaaatca acctctggat
     6661 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     6721 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     6781 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     6841 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     6901 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     6961 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     7021 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc
     7081 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     7141 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     7201 acgagtcgga tctccctttg ggccgcctcc ccgcctggta cctttaagac caatgactta
     7261 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     7321 tcactcccaa cgaagataag atctgctttt tgcttgtact gggtctctct ggttagacca
     7381 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     7441 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     7501 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct
     7561 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt
     7621 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     7681 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     7741 ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     7801 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     7861 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct agacttttgc
     7921 agagaccaaa ttcgtaatca tgtcatagct gtttcctgtg tgaaattgtt atccgctcac
     7981 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     8041 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     8101 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     8161 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     8221 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     8281 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     8341 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     8401 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     8461 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     8521 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     8581 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     8641 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     8701 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     8761 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
     8821 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     8881 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     8941 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     9001 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     9061 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     9121 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     9181 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     9241 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     9301 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     9361 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     9421 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     9481 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     9541 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     9601 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     9661 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     9721 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     9781 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     9841 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     9901 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     9961 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
    10021 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
    10081 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
    10141 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca
    10201 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
    10261 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc
    10321 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag
    10381 gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg
    10441 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
    10501 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtgc
    10561 caagctg
//
