LOCUS       V025811                11468 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V025811
VERSION     V025811
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..5511
                     /label="CP(NM_000096)"
                     /note="CP(NM_000096)"
                     /gene="CP"
     CDS             5518..5583
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5627..5838
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5851..6119
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      6148..6157
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6178..6840
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6910..6963
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6964..7563
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7564..8151
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8225..8458
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8530..8651
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8670..8805
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8843..8859)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8867..8883
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8891..8921)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8936..8957
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9245..9833)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10004..10864)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10865..10969)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11443..11459
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatga agattttgat acttggtatt
     2341 tttctgtttt tatgtagtac cccagcctgg gcgaaagaaa agcattatta cattggaatt
     2401 attgaaacga cttgggatta tgcctctgac catggggaaa agaaacttat ttctgttgac
     2461 acggaacatt ccaatatcta tcttcaaaat ggcccagata gaattgggag actatataag
     2521 aaggcccttt atcttcagta cacagatgaa acctttagga caactataga aaaaccggtc
     2581 tggcttgggt ttttaggccc tattatcaaa gctgaaactg gagataaagt ttatgtacac
     2641 ttaaaaaacc ttgcctctag gccctacacc tttcattcac atggaataac ttactataag
     2701 gaacatgagg gggccatcta ccctgataac accacagatt ttcaaagagc agatgacaaa
     2761 gtatatccag gagagcagta tacatacatg ttgcttgcca ctgaagaaca aagtcctggg
     2821 gaaggagatg gcaattgtgt gactaggatt taccattccc acattgatgc tccaaaagat
     2881 attgcctcag gactcatcgg acctttaata atctgtaaaa aagattctct agataaagaa
     2941 aaagaaaaac atattgaccg agaatttgtg gtgatgtttt ctgtggtgga tgaaaatttc
     3001 agctggtacc tagaagacaa cattaaaacc tactgctcag aaccagagaa agttgacaaa
     3061 gacaacgaag acttccagga gagtaacaga atgtattctg tgaatggata cacttttgga
     3121 agtctcccag gactctccat gtgtgctgaa gacagagtaa aatggtacct ttttggtatg
     3181 ggtaatgaag ttgatgtgca cgcagctttc tttcacgggc aagcactgac taacaagaac
     3241 taccgtattg acacaatcaa cctctttcct gctaccctgt ttgatgctta tatggtggcc
     3301 cagaaccctg gagaatggat gctcagctgt cagaatctaa accatctgaa agccggtttg
     3361 caagcctttt tccaggtcca ggagtgtaac aagtcttcat caaaggataa tatccgtggg
     3421 aagcatgtta gacactacta cattgccgct gaggaaatca tctggaacta tgctccctct
     3481 ggtatagaca tcttcactaa agaaaactta acagcacctg gaagtgactc agcggtgttt
     3541 tttgaacaag gtaccacaag aattggaggc tcttataaaa agctggttta tcgtgagtac
     3601 acagatgcct ccttcacaaa tcgaaaggag agaggccctg aagaagagca tcttggcatc
     3661 ctgggtcctg tcatttgggc agaggtggga gacaccatca gagtaacctt ccataacaaa
     3721 ggagcatatc ccctcagtat tgagccgatt ggggtgagat tcaataagaa caacgagggc
     3781 acatactatt ccccaaatta caacccccag agcagaagtg tgcctccttc agcctcccat
     3841 gtggcaccca cagaaacatt cacctatgaa tggactgtcc ccaaagaagt aggacccact
     3901 aatgcagatc ctgtgtgtct agctaagatg tattattctg ctgtggaacc cactaaagat
     3961 atattcactg ggcttattgg gccaatgaaa atatgcaaga aaggaagttt acatgcaaat
     4021 gggagacaga aagatgtaga caaggaattc tatttgtttc ctacagtatt tgatgagaat
     4081 gagagtttac tcctggaaga taatattaga atgtttacaa ctgcacctga tcaggtggat
     4141 aaggaagatg aagactttca ggaatctaat aaaatgcact ccatgaatgg attcatgtat
     4201 gggaatcagc cgggtctcac tatgtgcaaa ggagattcgg tcgtgtggta cttattcagc
     4261 gccggaaatg aggccgatgt acatggaata tacttttcag gaaacacata tctgtggaga
     4321 ggagaacgga gagacacagc aaacctcttc cctcaaacaa gtcttacgct ccacatgtgg
     4381 cctgacacag aggggacttt taatgttgaa tgccttacaa ctgatcatta cacaggcggc
     4441 atgaagcaaa aatatactgt gaaccaatgc aggcggcagt ctgaggattc caccttctac
     4501 ctgggagaga ggacatacta tatcgcagca gtggaggtgg aatgggatta ttccccacaa
     4561 agggagtggg aaaaggagct gcatcattta caagagcaga atgtttcaaa tgcattttta
     4621 gataagggag agttttacat aggctcaaag tacaagaaag ttgtgtatcg gcagtatact
     4681 gatagcacat tccgtgttcc agtggagaga aaagctgaag aagaacatct gggaattcta
     4741 ggtccacaac ttcatgcaga tgttggagac aaagtcaaaa ttatctttaa aaacatggcc
     4801 acaaggccct actcaataca tgcccatggg gtacaaacag agagttctac agttactcca
     4861 acattaccag gtgaaactct cacttacgta tggaaaatcc cagaaagatc tggagctgga
     4921 acagaggatt ctgcttgtat tccatgggct tattattcaa ctgtggatca agttaaggac
     4981 ctctacagtg gattaattgg ccccctgatt gtttgtcgaa gaccttactt gaaagtattc
     5041 aatcccagaa ggaaactgga atttgccctt ctgtttctag tttttgatga gaatgaatct
     5101 tggtacttag atgacaacat caaaacatac tctgatcacc ccgagaaagt aaacaaagat
     5161 gatgaggaat tcatagaaag caataaaatg catgctatta atggaagaat gtttggaaac
     5221 ctacaaggcc tcacaatgca cgtgggagat gaagtcaact ggtatctgat gggaatgggc
     5281 aatgaaatag acttacacac tgtacatttt cacggccata gcttccaata caagcacagg
     5341 ggagtttata gttctgatgt ctttgacatt ttccctggaa cataccaaac cctagaaatg
     5401 tttccaagaa cacctggaat ttggttactc cactgccatg tgaccgacca cattcatgct
     5461 ggaatggaaa ccacttacac cgttctacaa aatgaagaca ccaaatctgg cggatccgac
     5521 tacaaagacc atgacggtga ttataaagat catgacatcg actacaagga tgacgatgac
     5581 aagtagcggc cgcgaaggat ctgcgatcgc tccggtgccc gtcagtgggc agagcgcaca
     5641 tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaacggg tgcctagaga
     5701 aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag
     5761 ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg
     5821 tttgccgcca gaacacagct gaagcttcga ggggctcgca tctctccttc acgcgcccgc
     5881 cgccctacct gaggccgcca tccacgccgg ttgagtcgcg ttctgccgcc tcccgcctgt
     5941 ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa agctcaggtc gagaccgggc
     6001 ctttgtccgg cgctcccttg gagcctacct agactcagcc ggctctccac gctttgcctg
     6061 accctgcttg ctcaactcta cgtctttgtt tcgttttctg ttctgcgccg ttacagatcc
     6121 aagctgtgac cggcgcctac gctagacgcc accatggaga gcgacgagag cggcctgccc
     6181 gccatggaga tcgagtgccg catcaccggc accctgaacg gcgtggagtt cgagctggtg
     6241 ggcggcggag agggcacccc caagcagggc cgcatgacca acaagatgaa gagcaccaaa
     6301 ggcgccctga ccttcagccc ctacctgctg agccacgtga tgggctacgg cttctaccac
     6361 ttcggcacct accccagcgg ctacgagaac cccttcctgc acgccatcaa caacggcggc
     6421 tacaccaaca cccgcatcga gaagtacgag gacggcggcg tgctgcacgt gagcttcagc
     6481 taccgctacg aggccggccg cgtgatcggc gacttcaagg tggtgggcac cggcttcccc
     6541 gaggacagcg tgatcttcac cgacaagatc atccgcagca acgccaccgt ggagcacctg
     6601 caccccatgg gcgataacgt gctggtgggc agcttcgccc gcaccttcag cctgcgcgac
     6661 ggcggctact acagcttcgt ggtggacagc cacatgcact tcaagagcgc catccacccc
     6721 agcatcctgc agaacggggg ccccatgttc gccttccgcc gcgtggagga gctgcacagc
     6781 aacaccgagc tgggcatcgt ggagtaccag cacgccttca agacccccat cgccttcgcc
     6841 agatcccgcg ctcagtcgtc caattctgcc gtggacggca ccgccggacc cggctccacc
     6901 ggatctcgcg agggcagagg aagtcttcta acatgcggtg acgtggagga gaatcccggc
     6961 cctatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc
     7021 gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg
     7081 gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc
     7141 gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg
     7201 gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc
     7261 ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag
     7321 gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg
     7381 ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc
     7441 ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc
     7501 gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc
     7561 tgaaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     7621 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     7681 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     7741 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     7801 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     7861 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     7921 ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg
     7981 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     8041 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     8101 ctccgccttc gccctcagac gagtcggatc tccctttggc cgcctccccg cctggtacct
     8161 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
     8221 ggactggaag ggctaattca ctcccaacga aaataagatc tgctttttgc ttgtactggg
     8281 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     8341 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     8401 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     8461 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag
     8521 agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     8581 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     8641 aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc agttccgccc
     8701 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     8761 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctagac ttttgcagag
     8821 acggcccaaa ttcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     8881 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     8941 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     9001 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     9061 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     9121 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     9181 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     9241 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     9301 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     9361 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     9421 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     9481 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     9541 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     9601 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     9661 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
     9721 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     9781 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     9841 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     9901 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     9961 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
    10021 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
    10081 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
    10141 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
    10201 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
    10261 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
    10321 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
    10381 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
    10441 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
    10501 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
    10561 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
    10621 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
    10681 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
    10741 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
    10801 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
    10861 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
    10921 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
    10981 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
    11041 ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac
    11101 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
    11161 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat
    11221 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa
    11281 ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc
    11341 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc
    11401 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg
    11461 ccaagctg
//
