LOCUS       V024199                10139 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V024199
VERSION     V024199
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4182
                     /label="COLGALT1(NM_024656)"
                     /note="COLGALT1(NM_024656)"
                     /gene="COLGALT1"
     CDS             4189..4254
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4298..4509
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4522..4790
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      4819..4828
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             4849..5511
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5581..5634
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5635..6234
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6235..6822
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6896..7129
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7201..7322
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7341..7476
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7514..7530)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7538..7554
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7562..7592)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7607..7628
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7916..8504)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8675..9535)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9536..9640)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10114..10130
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg cggcggcccc acgcgcgggc
     2341 cggcggcgcg ggcagccgct cctggcgctg ctgcttctgc tgctggcgcc actgccgccg
     2401 ggggccccgc cgggcgccga cgcctacttc cccgaggagc gctggagccc ggagtcgccc
     2461 ctgcaggcgc cgcgcgtgct catcgcgctg ttggcgcgaa acgcggccca cgcgttgccc
     2521 accacgctgg gcgcactcga gcggctgcgg cacccgcggg agcgcacggc gctatgggtg
     2581 gctacggacc acaacatgga taacacgtca actgtgctgc gggagtggct ggtggccgtg
     2641 aagagtttgt accattccgt ggagtggcgg ccagcagagg agcccaggtc ctacccggac
     2701 gaggaaggcc cgaaacactg gtctgactca cgctacgagc atgtcatgaa gttgcgccag
     2761 gcagccctga aatcagctcg agacatgtgg gctgattaca tcctgtttgt agatgcggac
     2821 aacctgatcc tcaaccctga cacactgagc ctgctcatcg ctgagaacaa gacggtggtc
     2881 gcccccatgc tggattcccg ggctgcgtac tccaacttct ggtgtggaat gacttcccag
     2941 ggctactaca agcgcacacc tgcctacatc cctatccgca agcgagaccg ccggggctgc
     3001 tttgcagttc ccatggtgca ctcgaccttc ctgatcgacc tgcggaaggc ggcgtccagg
     3061 aacctggcct tctacccacc tcaccctgac tacacctggt cctttgacga catcatcgtc
     3121 tttgccttct cctgcaagca ggcagaggtt cagatgtatg tgtgcaacaa ggaggagtac
     3181 ggattcttgc cagtgccatt gcgcgcccac agcaccctcc aggatgaggc cgagagcttc
     3241 atgcatgtgc agctggaggt catggtgaag cacccgcccg cagagccctc ccgcttcatc
     3301 tcggctccca ccaagacacc ggacaagatg ggcttcgacg aggtcttcat gatcaacctg
     3361 aggcggcggc aggaccggcg ggagcgcatg ctgcgggcgc tgcaggcaca ggagatcgag
     3421 tgccggctgg tggaggccgt ggacggcaaa gccatgaaca ccagccaggt ggaggcgctg
     3481 gggatccaga tgctgcctgg ctaccgggac ccctaccacg gccggcccct caccaagggt
     3541 gagctgggct gcttcctgag ccactacaac atctggaagg aggtggtgga ccgggggctg
     3601 cagaaatcgc ttgtgtttga ggatgacctg cgttttgaga tcttcttcaa gagacgtctg
     3661 atgaacctca tgcgggatgt ggagcgggag ggcctggact gggacctcat ctatgtgggc
     3721 cggaagcgga tgcaggtgga gcaccccgag aaggctgtgc ctcgcgtgag gaacctggtg
     3781 gaggccgact attcctactg gaccctggcc tacgtgatct ccctgcaagg cgcccgcaaa
     3841 ctgctggctg ctgagccgct ctccaagatg ctgcctgtgg acgagttcct gcccgtcatg
     3901 ttcgacaaac acccagtgtc cgagtacaag gcccacttct ccctccgcaa cctgcatgcc
     3961 ttctctgtgg agccgctgct catctacccc acacactaca caggagacga tggctatgtg
     4021 agtgacaccg agacctcagt cgtatggaac aatgagcacg tcaagaccga ctgggaccgc
     4081 gccaagtccc agaagatgcg ggagcagcag gcactgagcc gtgaggccaa gaactcggac
     4141 gtgctccagt ccccactgga cagtgctgcc cgggatgaac tcggatccga ctacaaagac
     4201 catgacggtg attataaaga tcatgacatc gactacaagg atgacgatga caagtagcgg
     4261 ccgcgaagga tctgcgatcg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca
     4321 gtccccgaga agttgggggg aggggtcggc aattgaacgg gtgcctagag aaggtggcgc
     4381 ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga
     4441 gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc
     4501 agaacacagc tgaagcttcg aggggctcgc atctctcctt cacgcgcccg ccgccctacc
     4561 tgaggccgcc atccacgccg gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc
     4621 tgaactgcgt ccgccgtcta ggtaagttta aagctcaggt cgagaccggg cctttgtccg
     4681 gcgctccctt ggagcctacc tagactcagc cggctctcca cgctttgcct gaccctgctt
     4741 gctcaactct acgtctttgt ttcgttttct gttctgcgcc gttacagatc caagctgtga
     4801 ccggcgccta cgctagacgc caccatggag agcgacgaga gcggcctgcc cgccatggag
     4861 atcgagtgcc gcatcaccgg caccctgaac ggcgtggagt tcgagctggt gggcggcgga
     4921 gagggcaccc ccaagcaggg ccgcatgacc aacaagatga agagcaccaa aggcgccctg
     4981 accttcagcc cctacctgct gagccacgtg atgggctacg gcttctacca cttcggcacc
     5041 taccccagcg gctacgagaa ccccttcctg cacgccatca acaacggcgg ctacaccaac
     5101 acccgcatcg agaagtacga ggacggcggc gtgctgcacg tgagcttcag ctaccgctac
     5161 gaggccggcc gcgtgatcgg cgacttcaag gtggtgggca ccggcttccc cgaggacagc
     5221 gtgatcttca ccgacaagat catccgcagc aacgccaccg tggagcacct gcaccccatg
     5281 ggcgataacg tgctggtggg cagcttcgcc cgcaccttca gcctgcgcga cggcggctac
     5341 tacagcttcg tggtggacag ccacatgcac ttcaagagcg ccatccaccc cagcatcctg
     5401 cagaacgggg gccccatgtt cgccttccgc cgcgtggagg agctgcacag caacaccgag
     5461 ctgggcatcg tggagtacca gcacgccttc aagaccccca tcgccttcgc cagatcccgc
     5521 gctcagtcgt ccaattctgc cgtggacggc accgccggac ccggctccac cggatctcgc
     5581 gagggcagag gaagtcttct aacatgcggt gacgtggagg agaatcccgg ccctatgacc
     5641 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc
     5701 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac
     5761 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
     5821 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc
     5881 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
     5941 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
     6001 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc
     6061 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc
     6121 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
     6181 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgaaatcaa
     6241 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     6301 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     6361 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     6421 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     6481 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     6541 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     6601 actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt
     6661 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     6721 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tctccgcctt
     6781 cgccctcaga cgagtcggat ctccctttgg ccgcctcccc gcctggtacc tttaagacca
     6841 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
     6901 gggctaattc actcccaacg aaaataagat ctgctttttg cttgtactgg gtctctctgg
     6961 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     7021 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     7081 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca
     7141 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg
     7201 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     7261 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     7321 tatcatgtct ggctctagct atcccgcccc taactccgcc cagttccgcc cattctccgc
     7381 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     7441 tattccagaa gtagtgagga ggcttttttg gaggcctaga cttttgcaga gacggcccaa
     7501 attcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     7561 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     7621 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     7681 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     7741 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     7801 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     7861 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     7921 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     7981 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     8041 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     8101 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     8161 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     8221 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     8281 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     8341 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     8401 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     8461 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     8521 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     8581 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     8641 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     8701 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     8761 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     8821 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     8881 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     8941 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     9001 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     9061 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     9121 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     9181 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     9241 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     9301 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     9361 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     9421 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     9481 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     9541 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     9601 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     9661 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
     9721 cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
     9781 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
     9841 gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga
     9901 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat
     9961 accgcatcag gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
    10021 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
    10081 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gccaagctg
//
