LOCUS       V047262                11464 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V047262
VERSION     V047262
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..6318
                     /label="CLSPN(NM_022111)"
                     /note="CLSPN(NM_022111)"
                     /gene="CLSPN"
     CDS             6325..6390
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        6434..6645
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6658..6926
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             6953..7552
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7559..8147
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8221..8454
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8526..8647
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8666..8801
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8839..8855)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8863..8879
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8887..8917)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8932..8953
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9241..9829)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10000..10860)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10861..10965)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11439..11455
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catgacaggc gaggtgggtt ctgaggttca cctagaaatc
     2341 aatgacccaa acgtcatttc acaagaggaa gcagatagtc cttcagatag tggacagggc
     2401 agctatgaaa caattggacc cttgagtgaa ggagattcag atgaagagat atttgtaagt
     2461 aagaagttga aaaacaggaa ggttctacaa gacagtgatt ccgaaacaga ggacacaaat
     2521 gcctctccag agaaaactac ctatgacagt gccgaggagg aaaataaaga gaatttatat
     2581 gctgggaaaa atacaaaaat caaaaggatt tacaaaactg tggcagacag tgatgaaagt
     2641 tacatggaaa agtctttgta tcaggaaaat cttgaagcgc aagtgaaacc ttgcttagag
     2701 ctgagtcttc agtctggaaa ctctacagac tttaccactg acagaaagag ttccaaaaag
     2761 cacatacatg ataaagaagg aactgcagga aaagcaaaag taaaatcaaa aagaagactt
     2821 gagaaagagg agagaaaaat ggaaaaaatt agacagctaa aaaagaagga aacaaaaaac
     2881 caggaagatg atgtagaaca gccatttaat gacagtggct gtcttcttgt ggataaagac
     2941 ctttttgaaa ctgggttgga ggatgaaaat aactctccat tggaagatga agagtcatta
     3001 gaatcaataa gagcagctgt aaaaaacaaa gtaaaaaagc acaagaaaaa agaaccatct
     3061 ttggagagtg gggtccattc atttgaggaa ggaagtgagt tatcaaaagg aaccacgagg
     3121 aaggaaagaa aggcagccag attaagtaaa gaagcattaa aacaactgca tagtgagact
     3181 cagcgcctta ttcgagagtc tgcactgaac cttccatatc atatgcctga gaataaaacc
     3241 attcatgatt tcttcaaacg taaaccccgg cccacttgcc acggaaatgc catggcacta
     3301 ttgaagtcat ctaaatatca gtcaagccat cacaaagaaa tcatagacac tgcaaatact
     3361 actgaaatga acagtgatca ccatagtaaa ggttctgagc agacaacagg tgcagaaaat
     3421 gaagtggaaa ctaatgcact ccctgtagtt tcaaaggaaa cccagatcat tactggatca
     3481 gatgagtctt gcaggaagga tttggtaaaa aatgaagagc tagaaattca ggagaaacag
     3541 aagcagagtg acattagacc ttcacctggg gacagctcag tgttgcaaca ggaatccaac
     3601 ttcctcggga acaatcacag tgaggaatgt caggttggag ggcttgtagc atttgaacct
     3661 catgccctgg agggtgaagg cccccaaaat ccagaagaaa cagatgagaa agtggaagag
     3721 cctgagcagc aaaataaatc atcagcagtt gggccacctg aaaaagtgag acggtttact
     3781 ctggatagac ttaagcaact gggagtagat gtttccatta aaccacggct aggtgctgat
     3841 gaagattcct ttgtgatact tgaacctgaa accaacagag aactggaagc cttgaagcag
     3901 cgtttctgga agcatgctaa tccagcagcc aaacccaggg ctggtcagac agtgaatgtg
     3961 aacgtcatag tgaaagacat gggcactgat ggaaaggaag agctaaaagc agatgtggta
     4021 cctgtgactt tagcacctaa gaagttggat ggagcaagcc acacaaaacc aggtgaaaag
     4081 cttcaggtgt taaaagctaa actgcaagaa gcaatgaaac tccgaaggtt tgaggagcgc
     4141 cagaagcgcc aagcactgtt taaattagat aatgaagatg ggtttgagga agaggaggag
     4201 gaagaggaag aaatgacaga tgagtctgag gaagatggag aagagaaggt agagaaagaa
     4261 gagaaagagg aagaactaga ggaagaggag gagaaagaag aggaggagga agaagaagga
     4321 aatcaggaga ctgcagaatt ccttcttagt agtgaagaaa tagaaacaaa agatgaaaaa
     4381 gaaatggata aagaaaataa tgatggcagt agtgaaattg gcaaggcagt tggcttcctc
     4441 tctgttccca agtctctctc atcagattct actttacttc tgtttaagga cagctcttcc
     4501 aagatgggtt actttcctac tgaagaaaaa tcagaaacag atgaaaactc aggcaagcag
     4561 cctagcaaac tggatgagga tgattcatgt tcattgctaa caaaggagag cagccacaat
     4621 agcagctttg agctgattgg ctccacgatt ccatcctatc agccttgcaa cagacaaaca
     4681 ggccgtggga ccagtttttt ccctacagca ggaggattca gatctccttc ccctgggcta
     4741 tttcgagcca gtttggtcag ctcagcttct aagagttcag ggaaactgtc tgagccttca
     4801 cttcccatag aggattccca ggatctgtat aacgcctccc cagagcctaa gacacttttc
     4861 ctaggagcag gagacttcca gttctgttta gaagatgaca ctcagagcca actgttggat
     4921 gcagatgggt tcttaaatgt tagaaaccac aggaatcagt accaagcttt gaagcctcga
     4981 ttgccattgg ccagtatgga tgagaatgcc atggatgcca acatggatga gctgttggat
     5041 ttgtgtactg gaaagttcac atctcaggct gaaaaacatc tacccaggaa gagtgacaag
     5101 aaagagaaca tggaggaact tctgaacctt tgttcaggaa aattcacttc tcaggatgcc
     5161 tccactccag cctcatcaga gttaaataaa caggagaagg agagcagcat gggtgatcca
     5221 atggaagaag cacttgctct ttgctcaggc tcttttccca cagacaagga agaggaagac
     5281 gaggaggagg aatttggaga ctttcggctt gtttcaaatg ataatgagtt tgatagtgat
     5341 gaggatgaac acagtgactc tggtaatgat ctggcactgg aagaccatga agatgatgat
     5401 gaagaagaac tcctgaagcg atctgagaag ttgaaaaggc aaatgaggtt gaggaaatac
     5461 ctggaggatg aggcagaggt gtcaggaagt gatgtgggaa gcgaagatga gtatgatggg
     5521 gaagaaattg atgaatatga agaggacgta attgatgaag tacttccttc tgatgaggaa
     5581 ctgcagagtc aaatcaagaa aatacacatg aaaactatgt tggatgatga taagcgacag
     5641 ctacgtttat accaagagag gtaccttgct gatggggatc tgcacagcga tggtcctggg
     5701 cgaatgagga agtttcgatg gaaaaacata gatgatgctt cccagatgga cttgttccac
     5761 agagactctg atgatgatca gactgaagaa cagcttgatg agtcagaagc caggtggagg
     5821 aaggagcgaa ttgaacgaga gcagtggctt cgggacatgg cacagcaggg gaaaattaca
     5881 gctgaagaag aagaagaaat tggggaggac agtcagttta tgatactggc caagaaagtt
     5941 acagccaaag cactgcagaa gaatgccagt cgccctatgg ttattcagga atcaaagtct
     6001 ttgctcagaa atccttttga agccatcaga ccaggaagtg ctcaacaggt gaagacaggc
     6061 tcactgctaa accagcccaa agctgtgctt cagaaactgg ctgctctctc tgaccataac
     6121 cccagtgctc ctcgaaattc aagaaacttt gtctttcata cactttctcc tgtcaaggct
     6181 gaggcggcaa aggaatcgtc taagtctcag gtaaagaaaa ggggtccatc tttcatgact
     6241 tctccttcac ctaagcacct caaaacagat gatagcactt caggattgac gcgaagcatc
     6301 ttcaaatatt tggagagcgg atccgactac aaagaccatg acggtgatta taaagatcat
     6361 gacatcgact acaaggatga cgatgacaag tagcggccgc gaaggatctg cgatcgctcc
     6421 ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg
     6481 gtcggcaatt gaacgggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc
     6541 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc
     6601 gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacagctgaa gcttcgaggg
     6661 gctcgcatct ctccttcacg cgcccgccgc cctacctgag gccgccatcc acgccggttg
     6721 agtcgcgttc tgccgcctcc cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta
     6781 agtttaaagc tcaggtcgag accgggcctt tgtccggcgc tcccttggag cctacctaga
     6841 ctcagccggc tctccacgct ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg
     6901 ttttctgttc tgcgccgtta cagatccaag ctgtgaccgg cgcctacgct agatgaccga
     6961 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc ccccgggccg tacgcaccct
     7021 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgacccgg accgccacat
     7081 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
     7141 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga
     7201 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct
     7261 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg
     7321 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt
     7381 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc
     7441 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga
     7501 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gagtcgacaa
     7561 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     7621 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     7681 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     7741 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     7801 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     7861 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     7921 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
     7981 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     8041 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     8101 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg gtacctttaa
     8161 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     8221 tggaagggct aattcactcc caacgaaaat aagatctgct ttttgcttgt actgggtctc
     8281 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     8341 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     8401 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta
     8461 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     8521 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     8581 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     8641 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccagtt ccgcccattc
     8701 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     8761 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagactttt gcagagacgg
     8821 cccaaattcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     8881 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     8941 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     9001 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     9061 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     9121 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     9181 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     9241 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     9301 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     9361 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     9421 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     9481 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     9541 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     9601 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     9661 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     9721 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     9781 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     9841 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     9901 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     9961 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
    10021 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
    10081 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
    10141 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
    10201 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
    10261 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
    10321 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
    10381 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
    10441 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
    10501 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
    10561 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
    10621 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
    10681 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
    10741 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
    10801 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
    10861 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
    10921 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
    10981 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
    11041 tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
    11101 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
    11161 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga
    11221 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag
    11281 aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc
    11341 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt
    11401 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa
    11461 gctg
//
