LOCUS       V033572                12687 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033572
VERSION     V033572
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2285..3934
                     /EC_number=""
                     /codon_start=1
                     /experiment=""
                     /gene="luc"
                     /product="firefly luciferase"
                     /protein_id=""
                     /transl_table=1
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
                     /label="luciferase"
     CDS             3941..7612
                     /label="CHL1(NM_006614)"
                     /note="CHL1(NM_006614)"
                     /gene="CHL1"
     promoter        7657..7868
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             7881..8149
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             8176..8775
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    8782..9370
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             9444..9677
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    9749..9870
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      9889..10024
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(10062..10078)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    10086..10102
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(10110..10140)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    10155..10176
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(10464..11052)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(11223..12083)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(12084..12188)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     12662..12678
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattgc
     2281 caccatggaa gacgccaaaa acataaagaa aggcccggcg ccattctatc cgctagagga
     2341 tggaaccgct ggagagcaac tgcataaggc tatgaagaga tacgccctgg ttcctggaac
     2401 aattgctttt acagatgcac atatcgaggt gaacatcacg tacgcggaat acttcgaaat
     2461 gtccgttcgg ttggcagaag ctatgaaacg atatgggctg aatacaaatc acagaatcgt
     2521 cgtatgcagt gaaaactctc ttcaattctt tatgccggtg ttgggcgcgt tatttatcgg
     2581 agttgcagtt gcgcccgcga acgacattta taatgaacgt gaattgctca acagtatgaa
     2641 catttcgcag cctaccgtag tgtttgtttc caaaaagggg ttgcaaaaaa ttttgaacgt
     2701 gcaaaaaaaa ttaccaataa tccagaaaat tattatcatg gattctaaaa cggattacca
     2761 gggatttcag tcgatgtaca cgttcgtcac atctcatcta cctcccggtt ttaatgaata
     2821 cgattttgta ccagagtcct ttgatcgtga caaaacaatt gcactgataa tgaactcctc
     2881 tggatctact gggttaccta agggtgtggc ccttccgcat agaactgcct gcgtcagatt
     2941 ctcgcatgcc agagatccta tttttggcaa tcaaatcatt ccggatactg cgattttaag
     3001 tgttgttcca ttccatcacg gttttggaat gtttactaca ctcggatatt tgatatgtgg
     3061 atttcgagtc gtcttaatgt atagatttga agaagagctg tttttacgat cccttcagga
     3121 ttacaaaatt caaagtgcgt tgctagtacc aaccctattt tcattcttcg ccaaaagcac
     3181 tctgattgac aaatacgatt tatctaattt acacgaaatt gcttctgggg gcgcacctct
     3241 ttcgaaagaa gtcggggaag cggttgcaaa acgcttccat cttccaggga tacgacaagg
     3301 atatgggctc actgagacta catcagctat tctgattaca cccgaggggg atgataaacc
     3361 gggcgcggtc ggtaaagttg ttccattttt tgaagcgaag gttgtggatc tggataccgg
     3421 gaaaacgctg ggcgttaatc agagaggcga attatgtgtc agaggaccta tgattatgtc
     3481 cggttatgta aacaatccgg aagcgaccaa cgccttgatt gacaaggatg gatggctaca
     3541 ttctggagac atagcttact gggacgaaga cgaacacttc ttcatagttg accgcttgaa
     3601 gtctttaatt aaatacaaag gataccaggt ggcccccgct gaattggagt cgatattgtt
     3661 acaacacccc aacatcttcg acgcgggcgt ggcaggtctt cccgacgatg acgccggtga
     3721 acttcccgcc gccgttgttg ttttggagca cggaaagacg atgacggaaa aagagatcgt
     3781 ggattacgtc gccagtcaag taacaaccgc gaaaaagttg cgcggaggag ttgtgtttgt
     3841 ggacgaagta ccgaaaggtc ttaccggaaa actcgacgca agaaaaatca gagagatcct
     3901 cataaaggcc aagaagggcg gaaagtccaa attggaattc atggagccgc ttttacttgg
     3961 aagaggacta atcgtatatc taatgttcct cctgttaaaa ttctcaaaag caattgaaat
     4021 accatcttca gttcaacagg ttccaacaat cataaaacag tcaaaagtcc aagttgcctt
     4081 tcccttcgat gagtattttc aaattgaatg tgaagctaaa ggaaatccag aaccaacatt
     4141 ttcgtggact aaggatggca acccttttta tttcactgac catcggataa ttccatcgaa
     4201 caattcagga acattcagga tcccaaacga ggggcacata tctcactttc aagggaaata
     4261 ccgctgcttt gcttcaaata aactgggaat cgctatgtca gaagaaatag aatttatagt
     4321 tccaagtgtt ccaaaattcc caaaagaaaa aattgaccct cttgaagtgg aggagggaga
     4381 tccaattgtc ctcccatgca atcctcccaa aggcctccca cctttacaca tttattggat
     4441 gaatattgaa ttagaacaca tcgaacaaga tgaaagagta tacatgagcc aaaagggaga
     4501 tctatacttc gcaaacgtgg aagaaaagga cagtcgcaat gactactgtt gctttgctgc
     4561 atttccaaga ttaaggacta ttgtacagaa aatgccaatg aaactaacag ttaacagttt
     4621 aaagcatgct aatgactcaa gttcatccac agaaattggt tccaaggcaa attccatcaa
     4681 gcaaagaaaa cccaaactgc tgttgcctcc cactgagagt ggcagtgagt cttcaattac
     4741 catcctcaaa ggggaaatct tgctgcttga gtgttttgct gaaggcttgc caactccaca
     4801 ggttgattgg aacaaaattg gtggtgactt accaaagggg agagaaacaa aagaaaatta
     4861 tggcaagact ttgaagatag agaatgtctc ctaccaggac aaaggaaatt atcgctgcac
     4921 agccagcaat ttcttgggaa cagccactca cgattttcac gttatagtag aagagcctcc
     4981 tcgctggaca aagaagcctc agagtgctgt gtatagcacc ggaagcaatg gcatcttgtt
     5041 atgtgaggct gaaggagaac ctcaacccac aatcaagtgg agagtcaatg gctccccagt
     5101 tgacaatcat ccatttgctg gtgatgttgt cttccccagg gaaatcagtt ttaccaacct
     5161 tcaaccaaat catactgctg tgtaccagtg tgaagcctca aatgtccatg gaactatcct
     5221 tgccaatgcc aatattgatg ttgtggatgt ccgtccattg atacaaacca aagatggaga
     5281 aaattacgct acagtggttg ggtacagtgc tttcttacat tgcgagttct ttgcttcacc
     5341 tgaggcagtc gtgtcctggc agaaggtgga agaagtgaaa cccctggagg gcaggcggta
     5401 tcatatctat gaaaatggca cattgcagat caacagaacc accgaagaag atgctgggtc
     5461 ttactcatgt tgggtagaaa atgctatagg aaaaactgca gtcacagcca atttggatat
     5521 tagaaatgct acaaaactta gagtttctcc taagaatcct cgtatcccca aattgcatat
     5581 gcttgaatta cattgtgaaa gcaaatgtga ctcacatttg aaacacagtt tgaagttgtc
     5641 ctggagtaaa gatggagaag cctttgaaat taatggcaca gaagatggca ggataattat
     5701 tgatggagct aatttgacca tatctaatgt aactttagag gaccaaggta tttactgctg
     5761 ttcagctcat actgctctag acagtgctgc cgatataact caagtaactg ttcttgatgt
     5821 tccggatcca ccagaaaacc ttcacttgtc tgaaagacag aacaggagtg ttcggctgac
     5881 ctgggaagct ggagctgacc acaacagcaa tattagcgag tatattgttg aatttgaagg
     5941 aaacaaagaa gagcctggaa ggtgggagga actgaccaga gtccaaggaa agaaaaccac
     6001 agttatctta cctttggctc catttgtgag ataccagttc agggtcatag ccgtgaacga
     6061 agtagggaga agtcagccta gccagccgtc agaccatcat gaaacaccac cagcagctcc
     6121 agataggaat ccacaaaaca taagggttca agcctctcaa cccaaggaaa tgattataaa
     6181 gtgggagcct ttgaaatcca tggagcagaa tggaccaggc ctagagtaca gagtgacctg
     6241 gaagccacag ggagccccag tggagtggga agaagaaaca gtcacaaacc acacattgcg
     6301 ggtgatgacg cctgctgtct atgcccctta tgatgtcaag gtccaggcta tcaatcaact
     6361 aggatctggg cctgaccctc agtcagtgac tctctattct ggagaagact atcctgatac
     6421 agctccagtg atccatgggg tggacgttat aaacagtaca ttagttaaag ttacctggtc
     6481 aacagttcca aaggacagag tacatggacg tctgaaaggc tatcagataa attggtggaa
     6541 aacaaaaagt ctgttggatg gaagaacaca tcccaaagaa gtgaacattc taagattttc
     6601 aggacaaaga aactctggaa tggttccttc cttagatgcc tttagtgaat ttcatttaac
     6661 agtcttagcc tataactcta aaggagctgg tcctgaaagt gagccttata tatttcaaac
     6721 accagaagga gtacctgaac agccaacttt tctaaaggtc atcaaagttg ataaagacac
     6781 tgccacttta tcttggggac tacctaagaa attaaatgga aacttaactg gctatctttt
     6841 gcaatatcag ataataaatg acacctacga gattggagaa ttaaatgata ttaacattac
     6901 aactccatca aagcccagct ggcacctctc aaacctgaat gcaactacca agtacaaatt
     6961 ctacttgagg gcttgcactt cacagggctg tggaaaaccg atcacggagg aaagctccac
     7021 cttaggagaa gggagtaaag gtatcgggaa gatatcagga gtaaatctta ctcaaaagac
     7081 tcacccaata gaggtatttg agccgggagc tgaacatata gttcgcctaa tgactaagaa
     7141 ttggggcgat aatgatagca tttttcaaga tgtaattgag acaagaggga gagaatatgc
     7201 tggtttatat gatgacatct ccactcaagg ctggtttatt ggactgatgt gtgcgattgc
     7261 tcttctcaca ctactattat taactgtttg ctttgtgaag aggaatagag gtggaaagta
     7321 ctcagttaaa gaaaaggaag atttgcatcc agacccagaa attcagtcag taaaagatga
     7381 aacctttggt gaatacagtg acagtgatga aaagcctctc aaaggaagcc ttcggtccct
     7441 taatagggat atgcagccta ctgaaagtgc tgacagctta gtcgaatacg gagagggaga
     7501 ccatggtctc ttcagtgaag atggatcatt tattggtgcc tacgctggat ctaaggagaa
     7561 gggatctgtt gaaagcaatg gaagttctac agcaactttt ccccttcggg cataagcggc
     7621 cgcgaaggat ctgcgatcgc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag
     7681 tccccgagaa gttgggggga ggggtcggca attgaacggg tgcctagaga aggtggcgcg
     7741 gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag
     7801 aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca
     7861 gaacacagct gaagcttcga ggggctcgca tctctccttc acgcgcccgc cgccctacct
     7921 gaggccgcca tccacgccgg ttgagtcgcg ttctgccgcc tcccgcctgt ggtgcctcct
     7981 gaactgcgtc cgccgtctag gtaagtttaa agctcaggtc gagaccgggc ctttgtccgg
     8041 cgctcccttg gagcctacct agactcagcc ggctctccac gctttgcctg accctgcttg
     8101 ctcaactcta cgtctttgtt tcgttttctg ttctgcgccg ttacagatcc aagctgtgac
     8161 cggcgcctac gctagatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac
     8221 gtcccccggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac
     8281 accgtcgacc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg
     8341 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc
     8401 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg
     8461 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg
     8521 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag
     8581 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg
     8641 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc
     8701 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc
     8761 aagcccggtg cctgagtcga caatcaacct ctggattaca aaatttgtga aagattgact
     8821 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     8881 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     8941 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     9001 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     9061 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     9121 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     9181 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     9241 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     9301 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     9361 gcctccccgc ctggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
     9421 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa aataagatct
     9481 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     9541 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     9601 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     9661 gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc
     9721 aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa
     9781 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     9841 tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta
     9901 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     9961 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
    10021 ggcctagact tttgcagaga cggcccaaat tcgtaatcat ggtcatagct gtttcctgtg
    10081 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
    10141 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
    10201 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
    10261 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
    10321 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
    10381 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
    10441 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
    10501 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
    10561 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
    10621 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
    10681 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
    10741 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
    10801 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
    10861 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
    10921 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
    10981 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
    11041 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
    11101 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
    11161 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
    11221 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
    11281 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
    11341 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
    11401 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
    11461 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
    11521 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
    11581 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
    11641 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
    11701 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
    11761 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
    11821 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
    11881 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
    11941 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
    12001 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
    12061 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
    12121 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
    12181 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
    12241 acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat
    12301 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
    12361 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
    12421 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa
    12481 ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc attcaggctg
    12541 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa
    12601 gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt
    12661 tgtaaaacga cggccagtgc caagctg
//
