LOCUS       V045678                10337 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V045678
VERSION     V045678
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..5169
                     /label="CHAF1A(NM_005483)"
                     /note="CHAF1A(NM_005483)"
                     /gene="CHAF1A"
     CDS             5176..5202
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             5206..5232
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             5236..5262
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     promoter        5307..5518
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5531..5799
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5826..6425
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6432..7020
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7094..7327
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7399..7520
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7539..7674
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7712..7728)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7736..7752
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7760..7790)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7805..7826
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8114..8702)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8873..9733)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9734..9838)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10312..10328
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catgctggag gagctggagt gcggggcgcc cggcgccagg
     2341 ggagccgcca cagccatgga ttgcaaagat agaccagctt ttccagttaa gaagttaata
     2401 caagcccgtc tgccgtttaa gcgcctgaat cttgtcccaa aggggaaagc cgatgacatg
     2461 tcagacgatc agggtacttc tgtgcaaagt aaaagccccg atttagaggc ctctttggac
     2521 accttggaaa acaactgtca tgtgggttct gacatagact ttagaccgaa acttgtcaac
     2581 gggaagggtc ccttagataa ctttttaaga aatagaatcg aaaccagtat tggccagagc
     2641 acagtcatca ttgatttgac agaggactcg aatgagcagc cagacagtct tgtggaccac
     2701 aataaactaa attctgaagc ctctccctcc agggaggcaa taaatggcca gcgagaagac
     2761 actggggatc agcaggggtt gttgaaggcc attcagaacg acaagttggc atttcctgga
     2821 gagacccttt cagacattcc ttgcaaaaca gaggaggagg gtgttggctg tggaggtgca
     2881 gggaggagag gcgactccca ggaatgttcg ccacggagct gcccggagct gacgagtggc
     2941 ccgagaatgt gccccagaaa ggagcaggac agttggagtg aagctggggg catcctgttc
     3001 aaagggaagg tgcctatggt ggtcttgcag gacatcttgg ctgtgagacc accgcaaatc
     3061 aagtcccttc cagccacacc ccaaggcaag aacatgaccc ctgagagtga ggtgctggaa
     3121 tctttccccg aagaagactc tgtactcagc cattcgtccc tgagctctcc ctcttccacc
     3181 agctcgcccg aggggccgcc tgctccccca aagcagcaca gcagtaccag tcccttcccc
     3241 acctccacgc ccctccgcag aataactaag aaattcgtca aaggctctac agagaagaac
     3301 aagctcagac tgcaaagaga tcaggagcgt ctgggcaagc agctcaagtt acgtgcagaa
     3361 agggaagaaa aggagaagct gaaagaggag gccaagcggg ccaaggagga ggccaagaag
     3421 aagaaggagg aagagaagga gcttaaggaa aaggagaggc gggagaagcg ggagaaggat
     3481 gagaaggaga aggcggagaa gcagcggctc aaggaggagc ggcgcaagga gagacaggaa
     3541 gccctggagg ctaaacttga ggaaaaaagg aaaaaggaag aagagaaacg gttaagagaa
     3601 gaagagaagc gcattaaagc agagaaggcc gaaatcacga ggttcttcca gaaaccaaag
     3661 actccacagg cccccaagac cctggccggc tcctgtggga agtttgcccc ctttgaaatt
     3721 aaagagcaca tggtcctggc ccctcggcgt cggaccgctt tccatccaga cctctgcagt
     3781 cagctggacc agctcctcca gcagcagagc ggcgagttct ccttcttgaa agacctcaaa
     3841 ggccggcagc ccctgaggtc cggacccacg cacgtttcca cccggaatgc agatattttt
     3901 aacagtgatg tcgtcatcgt ggagcgtggg aagggcgacg gtgttcccga gaggaggaag
     3961 tttggcagga tgaagctcct gcagttctgt gagaaccacc ggcctgccta ctggggtacc
     4021 tggaataaga agacggcact catccgcgcg cgagacccct gggcccagga cacgaagctc
     4081 ctggactatg aggtggacag tgatgaggag tgggaagaag aggagcctgg ggagtccctg
     4141 tcccacagtg agggggatga tgatgacgac atgggagagg atgaagatga ggacgatggt
     4201 ttctttgtgc cccatgggta cctgtctgag gacgaaggtg tgacagagga gtgtgccgac
     4261 cctgagaacc ataaggtccg ccagaaactg aaggccaagg agtgggacga gttcctggct
     4321 aaggggaagc gctttcgcgt cctgcaacct gtgaagatcg gctgcgtgtg ggcggctgac
     4381 agagactgcg caggcgatga cctgaaggta ctgcagcagt tcgcagcctg cttcctggag
     4441 accctgccgg cccaggagga gcagacgccc aaggcctcca agcgggagag gagagacgag
     4501 cagatcctgg cccagctgct gccgctcctg cacggcaatg tgaacgggag caaggtcatc
     4561 atccgggagt tccaggagca ctgccgccgg ggactgctca gcaaccacac cggcagcccg
     4621 cggagcccct ccaccaccta cctgcacacc cccaccccca gcgaggatgc cgccatcccc
     4681 tctaagtccc ggctcaagcg gctcatttcc gagaactcag tgtatgagaa gcggcctgac
     4741 ttcaggatgt gctggtacgt gcacccgcag gtgctacaga gcttccagca ggagcacctg
     4801 cccgtgccgt gccagtggag ctatgtgaca tcggtgccct cggcccccaa agaggacagt
     4861 ggcagcgtcc cctccacggg gcccagccag ggcactccca tctcgctgaa gaggaagtca
     4921 gcgggcagca tgtgcatcac ccaattcatg aagaagcgca ggcacgacgg ccagattggt
     4981 gctgaagaca tggacggctt ccaggcagac acggaggagg aggaagagga ggagggcgac
     5041 tgtatgatcg tggatgtccc ggatgctgcg gaggtccaag ccccgtgtgg agccgcttcc
     5101 ggagctgggg gtggtgtggg ggtggacacc ggcaaggcca ccctgaccgc gagcccactg
     5161 ggtgcatccg gatcctaccc atacgacgta cctgattatg caacttatcc ttatgatgta
     5221 cctgattatg ctagctatcc ttatgatgtc ccagactacg cataggcggc cgcgaaggat
     5281 ctgcgatcgc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa
     5341 gttgggggga ggggtcggca attgaacggg tgcctagaga aggtggcgcg gggtaaactg
     5401 ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat
     5461 aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacagct
     5521 gaagcttcga ggggctcgca tctctccttc acgcgcccgc cgccctacct gaggccgcca
     5581 tccacgccgg ttgagtcgcg ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc
     5641 cgccgtctag gtaagtttaa agctcaggtc gagaccgggc ctttgtccgg cgctcccttg
     5701 gagcctacct agactcagcc ggctctccac gctttgcctg accctgcttg ctcaactcta
     5761 cgtctttgtt tcgttttctg ttctgcgccg ttacagatcc aagctgtgac cggcgcctac
     5821 gctagatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtcccccggg
     5881 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgacc
     5941 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc
     6001 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc
     6061 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga
     6121 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca
     6181 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc
     6241 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct
     6301 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca
     6361 ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg
     6421 cctgagtcga caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     6481 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     6541 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     6601 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     6661 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     6721 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     6781 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
     6841 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     6901 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     6961 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     7021 ctggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     7081 gaaaaggggg gactggaagg gctaattcac tcccaacgaa aataagatct gctttttgct
     7141 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     7201 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     7261 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     7321 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
     7381 atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     7441 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     7501 aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca
     7561 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     7621 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctagact
     7681 tttgcagaga cggcccaaat tcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     7741 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     7801 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     7861 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     7921 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     7981 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     8041 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     8101 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     8161 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     8221 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     8281 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     8341 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     8401 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     8461 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     8521 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     8581 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     8641 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     8701 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     8761 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     8821 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     8881 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     8941 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     9001 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     9061 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     9121 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     9181 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     9241 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     9301 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     9361 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     9421 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     9481 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     9541 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     9601 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     9661 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     9721 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     9781 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     9841 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     9901 ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa
     9961 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
    10021 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact
    10081 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
    10141 gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt
    10201 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg
    10261 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga
    10321 cggccagtgc caagctg
//
