LOCUS       V028019                11382 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V028019
VERSION     V028019
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2303..5464
                     /label="CEBPZ(NM_005760)"
                     /note="CEBPZ(NM_005760)"
                     /gene="CEBPZ"
     CDS             5471..5536
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5580..5791
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5804..6072
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             6107..6814
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             6824..6877
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6878..7477
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7478..8065
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8139..8372
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8444..8565
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8584..8719
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8757..8773)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8781..8797
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8805..8835)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8850..8871
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9159..9747)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9918..10778)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10779..10883)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11357..11373
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc gaattcgcca ccatggccgc agtcaaggag cctttggagt tccatgccaa
     2341 gcggccttgg cgccccgagg aggcagtaga agatccggac gaggaggatg aggataatac
     2401 tagtgaagcc gagaatgggt tctccctgga ggaagtgtta cggctcggag gcaccaagca
     2461 agattacctt atgctggcta ctttggatga gaatgaggaa gtgatagatg gaggcaaaaa
     2521 aggagcaatc gatgaccttc agcaaggtga attggaagca tttattcaaa atcttaattt
     2581 ggcgaagtat acaaaagctt ccttagttga agaagatgaa ccagctgaaa aagaaaattc
     2641 cagcaaaaaa gaagtaaaaa tacctaaaat aaataataaa aatacagcag aaagtcaaag
     2701 gacatcagtt aataaggtga aaaataagaa taggccagaa ccacattctg atgagaatgg
     2761 cagtaccaca ccgaaagtaa agaaagataa acagaacatc tttgaatttt ttgagagaca
     2821 gactttgtta cttaggcctg gaggcaaatg gtatgatctg gagtacagca atgaatattc
     2881 tttgaaaccc cagcctcagg atgttgtatc taagtacaaa acccttgctc agaagctgta
     2941 tcagcatgaa atcaacttat tcaaaagtaa gacgaatagt caaaagggag cctcttctac
     3001 ctggatgaag gcaattgtgt catcggggac actaggtgac aggatggcag ccatgattct
     3061 tcttattcag gatgatgccg ttcacacact tcagtttgta gaaactcttg tgaaccttgt
     3121 taaaaagaag ggcagcaaac agcagtgcct tatggccttg gatactttca aagagttgct
     3181 tatcacagac cttttgccag acaatcggaa gctgaggatt ttcagccagc gtccttttga
     3241 caaactggaa cagttgtcca gtggcaacaa ggactcaaga gatagaagac tgatattatg
     3301 gtattttgaa caccagctga aacacttagt ggctgaattt gtgcaggtct tagaaacttt
     3361 aagtcatgat acattagtaa ccactaaaac tcgagccctt accgtggctc atgagctgct
     3421 ttgtaacaag cctgaggaag aaaaggctct tcttgtgcaa gtggtaaata aactgggaga
     3481 tcctcagaac agaattgcca caaaagcatc ccatctgtta gagacattac tttgtaaaca
     3541 tcccaatatg aaaggagttg tgtctggtga agtagaaagg ctactcttcc gctcaaatat
     3601 cagctccaaa gctcaatatt atgcaatttg ctttttaaat caaatggctc tgtcccatga
     3661 agaaagtgaa ttggctaaca aattaataac tgtttacttt tgcttttttc ggacttgtgt
     3721 caaaaaaaaa gatgttgaat caaaaatgct tagcgccctt ttaacaggtg tgaatagggc
     3781 atacccttat tcccagactg gtgatgacaa agtaagggag cagattgaca cactgtttaa
     3841 agtgttgcat attgtgaatt ttaataccag tgtccaggct ttaatgttgc ttttccaagt
     3901 aatgaattct cagcagacaa tatcggatcg atattacaca gcattataca ggaagatgtt
     3961 ggatccaggg ttgatgacgt gttccaagca agctatgttt cttaaccttg tctacaaatc
     4021 tctgaaagct gacattgtgt tgcgccgggt gaaggctttt gtgaagaggt tacttcaagt
     4081 tacttgtcaa cagatgccac catttatatg tggagcttta tatcttgtgt ctgagatcct
     4141 taaagcaaaa ccaggtttaa gaagccaact agatgatcat ccggagtctg atgatgaaga
     4201 aaattttatt gatgcaaatg atgatgaaga catggaaaaa ttcactgatg cagacaaaga
     4261 aacagagata gtgaaaaaac ttgagacaga ggaaacagtt cctgaaactg atgtagaaac
     4321 caaaaaacca gaggttgctt cctgggtgca ctttgataat ttgaaaggtg ggaaacagtt
     4381 aaataaatac gatccattca gtagaaaccc tctgttctgt ggagctgaaa atacaagtct
     4441 ttgggaactc aaaaagttat ctgtgcattt tcatccctcc gtggcccttt ttgcaaagac
     4501 catccttcag ggaaactata ttcagtattc aggggaccca ctgcaggatt tcactctaat
     4561 gagatttttg gatcgatttg tataccgaaa tccaaagccc cataaaggca aagaaaacac
     4621 agatagtgtt gtgatgcagc cgaaaagaaa acattttatt aaggatattc gtcatcttcc
     4681 tgtgaacagt aaggagttcc ttgcaaaaga agaaagccaa ataccagtgg atgaagtgtt
     4741 tttccacagg tattataaaa aagttgctgt taaagagaaa caaaaacggg atgcagatga
     4801 agaaagtata gaagacgtgg atgatgaaga atttgaagag ctgattgaca catttgaaga
     4861 tgataactgt ttcagctctg gaaaggatga tatggatttt gctggaaacg tgaaaaagag
     4921 aacaaaagga gctaaggata acacattaga tgaagattca gaaggtagtg atgatgaact
     4981 tggtaacctg gatgacgatg aagtttcttt aggaagtatg gatgatgaag aatttgctga
     5041 agttgatgaa gatggaggaa cattcatgga tgtgttagat gatgaaagtg agagcgttcc
     5101 agaacttgaa gtccactcca aagtcagtac taagaaaagc aagagaaaag gtacagatga
     5161 ttttgacttt gctggctcat ttcaagggcc aagaaaaaag aaaagaaact taaatgattc
     5221 cagcctattt gtatctgctg aagagtttgg ccatctattg gatgaaaata tgggatccaa
     5281 gtttgataac attggcatga atgccatggc taacaaagat aatgcaagtc tcaaacagct
     5341 tagatgggag gctgaacgtg atgactggct acacaacaga gatgcaaaaa gtatcatcaa
     5401 gaaaaagaaa cattttaaaa agaagaggat taaaaccact caaaaaacta aaaaacaaag
     5461 gaaaggatcc gactacaaag accatgacgg tgattataaa gatcatgaca tcgactacaa
     5521 ggatgacgat gacaagtagc ggccgcgaag gatctgcgat cgctccggtg cccgtcagtg
     5581 ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac
     5641 gggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg
     5701 cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct
     5761 ttttcgcaac gggtttgccg ccagaacaca gctgaagctt cgaggggctc gcatctctcc
     5821 ttcacgcgcc cgccgcccta cctgaggccg ccatccacgc cggttgagtc gcgttctgcc
     5881 gcctcccgcc tgtggtgcct cctgaactgc gtccgccgtc taggtaagtt taaagctcag
     5941 gtcgagaccg ggcctttgtc cggcgctccc ttggagccta cctagactca gccggctctc
     6001 cacgctttgc ctgaccctgc ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg
     6061 ccgttacaga tccaagctgt gaccggcgcc tacgctagac gccaccatgg tgagcaaggg
     6121 cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg
     6181 ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg
     6241 cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat
     6301 cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc
     6361 cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga
     6421 ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta
     6481 caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac
     6541 catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga
     6601 gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac
     6661 ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga
     6721 catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg
     6781 ccactccacc ggcggcatgg acgagctgta caagggatct cgcgagggca gaggaagtct
     6841 tctaacatgc ggtgacgtgg aggagaatcc cggccctatg accgagtaca agcccacggt
     6901 gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc
     6961 cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga
     7021 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
     7081 cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
     7141 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
     7201 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
     7261 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
     7321 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct
     7381 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
     7441 gcgcacctgg tgcatgaccc gcaagcccgg tgcctgaaat caacctctgg attacaaaat
     7501 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     7561 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     7621 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     7681 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     7741 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     7801 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     7861 gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct
     7921 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     7981 cggcctgctg ccggctctgc ggcctcttcc gcgtctccgc cttcgccctc agacgagtcg
     8041 gatctccctt tggccgcctc cccgcctggt acctttaaga ccaatgactt acaaggcagc
     8101 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
     8161 acgaaaataa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc
     8221 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     8281 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     8341 acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca
     8401 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc
     8461 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     8521 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta
     8581 gctatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     8641 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     8701 ggaggctttt ttggaggcct agacttttgc agagacggcc caaattcgta atcatggtca
     8761 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     8821 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     8881 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     8941 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     9001 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     9061 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     9121 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     9181 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     9241 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     9301 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     9361 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     9421 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     9481 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     9541 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     9601 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     9661 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     9721 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     9781 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     9841 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     9901 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     9961 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
    10021 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
    10081 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
    10141 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
    10201 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
    10261 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
    10321 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
    10381 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
    10441 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
    10501 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
    10561 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
    10621 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
    10681 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
    10741 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
    10801 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
    10861 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
    10921 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc
    10981 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
    11041 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt
    11101 ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac
    11161 catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgccat
    11221 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
    11281 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
    11341 tcccagtcac gacgttgtaa aacgacggcc agtgccaagc tg
//
