LOCUS       V024183                12743 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V024183
VERSION     V024183
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..6786
                     /label="CDK12(NM_016507)"
                     /note="CDK12(NM_016507)"
                     /gene="CDK12"
     CDS             6793..6858
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        6902..7113
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             7126..7394
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      7423..7432
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             7453..8115
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             8185..8238
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             8239..8838
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    8839..9426
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             9500..9733
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    9805..9926
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      9945..10080
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(10118..10134)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    10142..10158
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(10166..10196)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    10211..10232
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(10520..11108)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(11279..12139)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(12140..12244)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     12718..12734
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgc ccaattcaga gagacatggg
     2341 ggcaagaagg acgggagtgg aggagcttct ggaactttgc agccgtcatc gggaggcggc
     2401 agctctaaca gcagagagcg tcaccgcttg gtatcgaagc acaagcggca taagtccaaa
     2461 cactccaaag acatggggtt ggtgaccccc gaagcagcat ccctgggcac agttatcaaa
     2521 cctttggtgg agtatgatga tatcagctct gattccgaca ccttctccga tgacatggcc
     2581 ttcaaactag accgaaggga gaacgacgaa cgtcgtggat cagatcggag cgaccgcctg
     2641 cacaaacatc gtcaccacca gcacaggcgt tcccgggact tactaaaagc taaacagacc
     2701 gaaaaagaaa aaagccaaga agtctccagc aagtcgggat cgatgaagga ccggatatcg
     2761 ggaagttcaa agcgttcgaa tgaggagact gatgactatg ggaaggcgca ggtagccaaa
     2821 agcagcagca aggaatccag gtcatccaag ctccacaagg agaagaccag gaaagaacgg
     2881 gagctgaagt ctgggcacaa agaccggagt aaaagtcatc gaaaaaggga aacacccaaa
     2941 agttacaaaa cagtggacag cccaaaacgg agatccagga gcccccacag gaagtggtct
     3001 gacagctcca aacaagatga tagcccctcg ggagcttctt atggccaaga ttatgacctt
     3061 agtccctcac gatctcatac ctcgagcaat tatgactcct acaagaaaag tcctggaagt
     3121 acctcgagaa ggcagtcggt cagtccccct tacaaggagc cttcggccta ccagtccagc
     3181 acccggtcac cgagccccta cagtaggcga cagagatctg tcagtcccta tagcaggaga
     3241 cggtcgtcca gctacgaaag aagtggctct tacagcgggc gatcgcccag tccctatggt
     3301 cgaaggcggt ccagcagccc tttcctgagc aagcggtctc tgagtcggag tccactcccc
     3361 agtaggaaat ccatgaagtc cagaagtaga agtcctgcat attcaagaca ttcatcttct
     3421 catagtaaaa agaagagatc cagttcacgc agtcgtcatt ccagtatctc acctgtcagg
     3481 cttccactta attccagtct gggagctgaa ctcagtagga aaaagaagga aagagcagct
     3541 gctgctgctg cagcaaagat ggatggaaag gagtccaagg gttcacctgt atttttgcct
     3601 agaaaagaga acagttcagt agaggctaag gattcaggtt tggagtctaa aaagttaccc
     3661 agaagtgtaa aattggaaaa atctgcccca gatactgaac tggtgaatgt aacacatcta
     3721 aacacagagg taaaaaattc ttcagataca gggaaagtaa agttggatga gaactccgag
     3781 aagcatcttg ttaaagattt gaaagcacag ggaacaagag actctaaacc catagcactg
     3841 aaagaggaga ttgttactcc aaaggagaca gaaacatcag aaaaggagac ccctccacct
     3901 cttcccacaa ttgcttctcc cccaccccct ctaccaacta ctacccctcc acctcagaca
     3961 ccccctttgc cacctttgcc tccaatacca gctcttccac agcaaccacc tctgcctcct
     4021 tctcagccag catttagtca ggttcctgct tccagtactt caactttgcc cccttctact
     4081 cactcaaaga catctgctgt gtcctctcag gcaaattctc agccccctgt acaggtttct
     4141 gtgaagactc aagtatctgt aacagctgct attccacacc tgaaaacttc aacgttgcct
     4201 cctttgcccc tcccaccctt attacctgga gatgatgaca tggatagtcc aaaagaaact
     4261 cttccttcaa aacctgtgaa gaaagagaag gaacagagga cacgtcactt actcacagac
     4321 cttcctctcc ctccagagct ccctggtgga gatctgtctc ccccagactc tccagaacca
     4381 aaggcaatca caccacctca gcaaccatat aaaaagagac caaaaatttg ttgtcctcgt
     4441 tatggagaaa gaagacaaac agaaagcgac tgggggaaac gctgtgtgga caagtttgac
     4501 attattggga ttattggaga aggaacctat ggccaagtat ataaagccaa ggacaaagac
     4561 acaggagaac tagtggctct gaagaaggtg agactagaca atgagaaaga gggcttccca
     4621 atcacagcca ttcgtgaaat caaaatcctt cgtcagttaa tccaccgaag tgttgttaac
     4681 atgaaggaaa ttgtcacaga taaacaagat gcactggatt tcaagaagga caaaggtgcc
     4741 ttttaccttg tatttgagta tatggaccat gacttaatgg gactgctaga atctggtttg
     4801 gtgcactttt ctgaggacca tatcaagtcg ttcatgaaac agctaatgga aggattggaa
     4861 tactgtcaca aaaagaattt cctgcatcgg gatattaagt gttctaacat tttgctgaat
     4921 aacagtgggc aaatcaaact agcagatttt ggacttgctc ggctctataa ctctgaagag
     4981 agtcgccctt acacaaacaa agtcattact ttgtggtacc gacctccaga actactgcta
     5041 ggagaggaac gttacacacc agccatagat gtttggagct gtggatgtat tcttggggaa
     5101 ctattcacaa agaagcctat ttttcaagcc aatctggaac tggctcagct agaactgatc
     5161 agccgacttt gtggtagccc ttgtccagct gtgtggcctg atgttatcaa actgccctac
     5221 ttcaacacca tgaaaccgaa gaagcaatat cgaaggcgtc tacgagaaga attctctttc
     5281 attccttctg cagcacttga tttattggac cacatgctga cactagatcc tagtaagcgg
     5341 tgcacagctg aacagaccct acagagcgac ttccttaaag atgtcgaact cagcaaaatg
     5401 gctcctccag acctccccca ctggcaggat tgccatgagt tgtggagtaa gaaacggcga
     5461 cgtcagcgac aaagtggtgt tgtagtcgaa gagccacctc catccaaaac ttctcgaaaa
     5521 gaaactacct cagggacaag tactgagcct gtgaagaaca gcagcccagc accacctcag
     5581 cctgctcctg gcaaggtgga gtctggggct ggggatgcaa taggccttgc tgacatcaca
     5641 caacagctga atcaaagtga attggcagtg ttattaaacc tgctgcagag ccaaaccgac
     5701 ctgagcatcc ctcaaatggc acagctgctt aacatccact ccaacccaga gatgcagcag
     5761 cagctggaag ccctgaacca atccatcagt gccctgacgg aagctacttc ccagcagcag
     5821 gactcagaga ccatggcccc agaggagtct ttgaaggaag caccctctgc cccagtgatc
     5881 ctgccttcag cagaacagac gacccttgaa gcttcaagca caccagctga catgcagaat
     5941 atattggcag ttctcttgag tcagctgatg aaaacccaag agccagcagg cagtctggag
     6001 gaaaacaaca gtgacaagaa cagtgggcca caggggcccc gaagaactcc cacaatgcca
     6061 caggaggagg cagcagcatg tcctcctcac attcttccac cagagaagag gccccctgag
     6121 ccccccggac ctccaccgcc gccacctcca ccccctctgg ttgaaggcga tctttccagc
     6181 gccccccagg agttgaaccc agccgtgaca gccgccttgc tgcaactttt atcccagcct
     6241 gaagcagagc ctcctggcca cctgccacat gagcaccagg ccttgagacc aatggagtac
     6301 tccacccgac cccgtccaaa caggacttat ggaaacactg atgggcctga aacagggttc
     6361 agtgccattg acactgatga acgaaactct ggtccagcct tgacagaatc cttggtccag
     6421 accctggtga agaacaggac cttctcaggc tctctgagcc accttgggga gtccagcagt
     6481 taccagggca cagggtcagt gcagtttcca ggggaccagg acctccgttt tgccagggtc
     6541 cccttagcgt tacacccggt ggtcgggcaa ccattcctga aggctgaggg aagcagcaat
     6601 tctgtggtac atgcagagac caaattgcaa aactatgggg agctggggcc aggaaccact
     6661 ggggccagca gctcaggagc aggccttcac tgggggggcc caactcagtc ttctgcttat
     6721 ggaaaactct atcgggggcc tacaagagtc ccaccaagag ggggaagagg gagaggagtt
     6781 ccttacggat ccgactacaa agaccatgac ggtgattata aagatcatga catcgactac
     6841 aaggatgacg atgacaagta gcggccgcga aggatctgcg atcgctccgg tgcccgtcag
     6901 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga
     6961 acgggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc
     7021 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt
     7081 ctttttcgca acgggtttgc cgccagaaca cagctgaagc ttcgaggggc tcgcatctct
     7141 ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg
     7201 ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc
     7261 aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc
     7321 tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg
     7381 cgccgttaca gatccaagct gtgaccggcg cctacgctag acgccaccat ggagagcgac
     7441 gagagcggcc tgcccgccat ggagatcgag tgccgcatca ccggcaccct gaacggcgtg
     7501 gagttcgagc tggtgggcgg cggagagggc acccccaagc agggccgcat gaccaacaag
     7561 atgaagagca ccaaaggcgc cctgaccttc agcccctacc tgctgagcca cgtgatgggc
     7621 tacggcttct accacttcgg cacctacccc agcggctacg agaacccctt cctgcacgcc
     7681 atcaacaacg gcggctacac caacacccgc atcgagaagt acgaggacgg cggcgtgctg
     7741 cacgtgagct tcagctaccg ctacgaggcc ggccgcgtga tcggcgactt caaggtggtg
     7801 ggcaccggct tccccgagga cagcgtgatc ttcaccgaca agatcatccg cagcaacgcc
     7861 accgtggagc acctgcaccc catgggcgat aacgtgctgg tgggcagctt cgcccgcacc
     7921 ttcagcctgc gcgacggcgg ctactacagc ttcgtggtgg acagccacat gcacttcaag
     7981 agcgccatcc accccagcat cctgcagaac gggggcccca tgttcgcctt ccgccgcgtg
     8041 gaggagctgc acagcaacac cgagctgggc atcgtggagt accagcacgc cttcaagacc
     8101 cccatcgcct tcgccagatc ccgcgctcag tcgtccaatt ctgccgtgga cggcaccgcc
     8161 ggacccggct ccaccggatc tcgcgagggc agaggaagtc ttctaacatg cggtgacgtg
     8221 gaggagaatc ccggccctat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
     8281 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
     8341 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
     8401 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
     8461 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
     8521 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
     8581 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac
     8641 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
     8701 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc
     8761 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
     8821 cgcaagcccg gtgcctgaaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     8881 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     8941 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     9001 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     9061 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     9121 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     9181 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg
     9241 tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     9301 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     9361 cggcctcttc cgcgtctccg ccttcgccct cagacgagtc ggatctccct ttggccgcct
     9421 ccccgcctgg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     9481 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaaaata agatctgctt
     9541 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     9601 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     9661 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     9721 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag
     9781 aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa
     9841 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     9901 ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc
     9961 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
    10021 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
    10081 tagacttttg cagagacggc ccaaattcgt aatcatggtc atagctgttt cctgtgtgaa
    10141 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
    10201 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
    10261 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
    10321 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
    10381 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
    10441 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
    10501 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
    10561 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
    10621 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
    10681 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
    10741 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
    10801 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
    10861 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
    10921 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
    10981 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
    11041 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
    11101 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
    11161 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
    11221 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
    11281 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
    11341 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
    11401 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
    11461 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
    11521 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
    11581 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
    11641 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
    11701 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
    11761 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
    11821 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
    11881 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
    11941 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
    12001 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
    12061 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
    12121 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
    12181 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
    12241 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat
    12301 taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg
    12361 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg
    12421 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc
    12481 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac
    12541 cgcacagatg cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca
    12601 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg
    12661 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
    12721 aaacgacggc cagtgccaag ctg
//
