LOCUS       V046678                10034 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V046678
VERSION     V046678
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     regulatory      2296..2305
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             3166..3219
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             3220..4206
                     /label="CD86(NM_175862)"
                     /note="CD86(NM_175862)"
                     /gene="CD86"
     CDS             4243..4953
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="enhanced monomeric blue fluorescent protein
                     (Subach et al., 2011)"
                     /transl_table=1
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
                     /label="mTagBFP2"
     promoter        5004..5215
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5228..5496
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5523..6122
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6129..6717
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6791..7024
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7096..7217
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7236..7371
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7409..7425)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7433..7449
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7457..7487)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7502..7523
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7811..8399)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8570..9430)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9431..9535)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10009..10025
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catgggccac acacggaggc agggaacatc accatccaag
     2341 tgtccatacc tcaatttctt tcagctcttg gtgctggctg gtctttctca cttctgttca
     2401 ggtgttatcc acgtgaccaa ggaagtgaaa gaagtggcaa cgctgtcctg tggtcacaat
     2461 gtttctgttg aagagctggc acaaactcgc atctactggc aaaaggagaa gaaaatggtg
     2521 ctgactatga tgtctgggga catgaatata tggcccgagt acaagaaccg gaccatcttt
     2581 gatatcacta ataacctctc cattgtgatc ctggctctgc gcccatctga cgagggcaca
     2641 tacgagtgtg ttgttctgaa gtatgaaaaa gacgctttca agcgggaaca cctggctgaa
     2701 gtgacgttat cagtcaaagc tgacttccct acacctagta tatctgactt tgaaattcca
     2761 acttctaata ttagaaggat aatttgctca acctctggag gttttccaga gcctcacctc
     2821 tcctggttgg aaaatggaga agaattaaat gccatcaaca caacagtttc ccaagatcct
     2881 gaaactgagc tctatgctgt tagcagcaaa ctggatttca atatgacaac caaccacagc
     2941 ttcatgtgtc tcatcaagta tggacattta agagtgaatc agaccttcaa ctggaataca
     3001 accaagcaag agcattttcc tgataacctg ctcccatcct gggccattac cttaatctca
     3061 gtaaatggaa tttttgtgat atgctgcctg acctactgct ttgccccaag atgcagagag
     3121 agaaggagga atgagagatt gagaagggaa agtgtacgcc ctgtagaggg cagaggaagt
     3181 cttctaacat gcggtgacgt ggaggagaat cccggcccta tggatcccca gtgcactatg
     3241 ggactgagta acattctctt tgtgatggcc ttcctgctct ctggtgctgc tcctctgaag
     3301 attcaagctt atttcaatga gactgcagac ctgccatgcc aatttgcaaa ctctcaaaac
     3361 caaagcctga gtgagctagt agtattttgg caggaccagg aaaacttggt tctgaatgag
     3421 gtatacttag gcaaagagaa atttgacagt gttcattcca agtatatggg ccgcacaagt
     3481 tttgattcgg acagttggac cctgagactt cacaatcttc agatcaagga caagggcttg
     3541 tatcaatgta tcatccatca caaaaagccc acaggaatga ttcgcatcca ccagatgaat
     3601 tctgaactgt cagtgcttgc taacttcagt caacctgaaa tagtaccaat ttctaatata
     3661 acagaaaatg tgtacataaa tttgacctgc tcatctatac acggttaccc agaacctaag
     3721 aagatgagtg ttttgctaag aaccaagaat tcaactatcg agtatgatgg tgttatgcag
     3781 aaatctcaag ataatgtcac agaactgtac gacgtttcca tcagcttgtc tgtttcattc
     3841 cctgatgtta cgagcaatat gaccatcttc tgtattctgg aaactgacaa gacgcggctt
     3901 ttatcttcac ctttctctat agagcttgag gaccctcagc ctcccccaga ccacattcct
     3961 tggattacag ctgtacttcc aacagttatt atatgtgtga tggttttctg tctaattcta
     4021 tggaaatgga agaagaagaa gcggcctcgc aactcttata aatgtggaac caacacaatg
     4081 gagagggaag agagtgaaca gaccaagaaa agagaaaaaa tccatatacc tgaaagatct
     4141 gatgaagccc agcgtgtttt taaaagttcg aagacatctt catgcgacaa aagtgataca
     4201 tgttttctcg agggtggagg cggatcagga ggcggtggat ctatggtgtc taagggcgaa
     4261 gagctgatta aggagaacat gcacatgaag ctgtacatgg agggcaccgt ggacaaccat
     4321 cacttcaagt gcacatccga gggcgaaggc aagccctacg agggcaccca gaccatgaga
     4381 atcaaggtgg tcgagggcgg ccctctcccc ttcgccttcg acatcctggc tactagcttc
     4441 ctctacggca gcaagacctt catcaaccac acccagggca tccccgactt cttcaagcag
     4501 tccttccctg agggcttcac atgggagaga gtcaccacat acgaagacgg gggcgtgctg
     4561 accgctaccc aggacaccag cctccaggac ggctgcctca tctacaacgt caagatcaga
     4621 ggggtgaact tcacatccaa cggccctgtg atgcagaaga aaacactcgg ctgggaggcc
     4681 ttcaccgaga cgctgtaccc cgctgacggc ggcctggaag gcagaaacga catggccctg
     4741 aagctcgtgg gcgggagcca tctgatcgca aacgccaaga ccacatatag atccaagaaa
     4801 cccgctaaga acctcaagat gcctggcgtc tactatgtgg actacagact ggaaagaatc
     4861 aaggaggcca acaacgagac ctacgtcgag cagcacgagg tggcagtggc cagatactgc
     4921 gacctcccta gcaaactggg gcacaagctt aattaaggat ccgcggccgc gaaggatctg
     4981 cgatcgctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt
     5041 ggggggaggg gtcggcaatt gaacgggtgc ctagagaagg tggcgcgggg taaactggga
     5101 aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag
     5161 tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacagctgaa
     5221 gcttcgaggg gctcgcatct ctccttcacg cgcccgccgc cctacctgag gccgccatcc
     5281 acgccggttg agtcgcgttc tgccgcctcc cgcctgtggt gcctcctgaa ctgcgtccgc
     5341 cgtctaggta agtttaaagc tcaggtcgag accgggcctt tgtccggcgc tcccttggag
     5401 cctacctaga ctcagccggc tctccacgct ttgcctgacc ctgcttgctc aactctacgt
     5461 ctttgtttcg ttttctgttc tgcgccgtta cagatccaag ctgtgaccgg cgcctacgct
     5521 agatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc ccccgggccg
     5581 tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgacccgg
     5641 accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg
     5701 acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg
     5761 agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg
     5821 gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg
     5881 agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg
     5941 gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc
     6001 tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg
     6061 ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct
     6121 gagtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     6181 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     6241 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     6301 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     6361 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     6421 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     6481 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt
     6541 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     6601 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     6661 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg
     6721 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     6781 aaggggggac tggaagggct aattcactcc caacgaaaat aagatctgct ttttgcttgt
     6841 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     6901 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     6961 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     7021 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata
     7081 tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     7141 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     7201 ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccagtt
     7261 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     7321 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagactttt
     7381 gcagagacgg cccaaattcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     7441 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     7501 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     7561 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     7621 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     7681 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     7741 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     7801 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     7861 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     7921 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     7981 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     8041 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     8101 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     8161 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     8221 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
     8281 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     8341 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     8401 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     8461 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     8521 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     8581 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     8641 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     8701 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     8761 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     8821 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     8881 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     8941 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     9001 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     9061 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     9121 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     9181 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     9241 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     9301 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     9361 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     9421 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     9481 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     9541 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
     9601 aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc
     9661 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
     9721 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg
     9781 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat
     9841 gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg
     9901 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg
     9961 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg
    10021 ccagtgccaa gctg
//
