LOCUS       V034858                 9891 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V034858
VERSION     V034858
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2303..3973
                     /label="CD19(NM_001178098)"
                     /note="CD19(NM_001178098)"
                     /gene="CD19"
     CDS             3980..4045
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4089..4300
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4313..4581
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             4616..5323
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             5333..5386
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5387..5986
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    5987..6574
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6648..6881
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    6953..7074
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7093..7228
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7266..7282)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7290..7306
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7314..7344)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7359..7380
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7668..8256)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8427..9287)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9288..9392)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     9866..9882
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc gaattcgcca ccatgccacc tcctcgcctc ctcttcttcc tcctcttcct
     2341 cacccccatg gaagtcaggc ccgaggaacc tctagtggtg aaggtggaag agggagataa
     2401 cgctgtgctg cagtgcctca aggggacctc agatggcccc actcagcagc tgacctggtc
     2461 tcgggagtcc ccgcttaaac ccttcttaaa actcagcctg gggctgccag gcctgggaat
     2521 ccacatgagg cccctggcca tctggctttt catcttcaac gtctctcaac agatgggggg
     2581 cttctacctg tgccagccgg ggcccccctc tgagaaggcc tggcagcctg gctggacagt
     2641 caatgtggag ggcagcgggg agctgttccg gtggaatgtt tcggacctag gtggcctggg
     2701 ctgtggcctg aagaacaggt cctcagaggg ccccagctcc ccttccggga agctcatgag
     2761 ccccaagctg tatgtgtggg ccaaagaccg ccctgagatc tgggagggag agcctccgtg
     2821 tctcccaccg agggacagcc tgaaccagag cctcagccag gacctcacca tggcccctgg
     2881 ctccacactc tggctgtcct gtggggtacc ccctgactct gtgtccaggg gccccctctc
     2941 ctggacccat gtgcacccca aggggcctaa gtcattgctg agcctagagc tgaaggacga
     3001 tcgcccggcc agagatatgt gggtaatgga gacgggtctg ttgttgcccc gggccacagc
     3061 tcaagacgct ggaaagtatt attgtcaccg tggcaacctg accatgtcat tccacctgga
     3121 gatcactgct cggccagtac tatggcactg gctgctgagg actggtggct ggaaggtctc
     3181 agctgtgact ttggcttatc tgatcttctg cctgtgttcc cttgtgggca ttcttcatct
     3241 tcaaagagcc ctggtcctga ggaggaaaag aaagcgaatg actgacccca ccaggagatt
     3301 cttcaaagtg acgcctcccc caggaagcgg gccccagaac cagtacggga acgtgctgtc
     3361 tctccccaca cccacctcag gcctcggacg cgcccagcgt tgggccgcag gcctgggggg
     3421 cactgccccg tcttatggaa acccgagcag cgacgtccag gcggatggag ccttggggtc
     3481 ccggagcccg ccgggagtgg gcccagaaga agaggaaggg gagggctatg aggaacctga
     3541 cagtgaggag gactccgagt tctatgagaa cgactccaac cttgggcagg accagctctc
     3601 ccaggatggc agcggctacg agaaccctga ggatgagccc ctgggtcctg aggatgaaga
     3661 ctccttctcc aacgctgagt cttatgagaa cgaggatgaa gagctgaccc agccggtcgc
     3721 caggacaatg gacttcctga gccctcatgg gtcagcctgg gaccccagcc gggaagcaac
     3781 ctccctggca gggtcccagt cctatgagga tatgagagga atcctgtatg cagcccccca
     3841 gctccgctcc attcggggcc agcctggacc caatcatgag gaagatgcag actcttatga
     3901 gaacatggat aatcccgatg ggccagaccc agcctgggga ggagggggcc gcatgggcac
     3961 ctggagcacc aggggatccg actacaaaga ccatgacggt gattataaag atcatgacat
     4021 cgactacaag gatgacgatg acaagtagcg gccgcgaagg atctgcgatc gctccggtgc
     4081 ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg
     4141 caattgaacg ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta
     4201 ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt
     4261 gaacgttctt tttcgcaacg ggtttgccgc cagaacacag ctgaagcttc gaggggctcg
     4321 catctctcct tcacgcgccc gccgccctac ctgaggccgc catccacgcc ggttgagtcg
     4381 cgttctgccg cctcccgcct gtggtgcctc ctgaactgcg tccgccgtct aggtaagttt
     4441 aaagctcagg tcgagaccgg gcctttgtcc ggcgctccct tggagcctac ctagactcag
     4501 ccggctctcc acgctttgcc tgaccctgct tgctcaactc tacgtctttg tttcgttttc
     4561 tgttctgcgc cgttacagat ccaagctgtg accggcgcct acgctagacg ccaccatggt
     4621 gagcaagggc gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca
     4681 catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc
     4741 ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc
     4801 ctgggacatc ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc
     4861 cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat
     4921 gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga
     4981 gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca
     5041 gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct
     5101 gaagggcgag atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt
     5161 caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat
     5221 caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc
     5281 cgagggccgc cactccaccg gcggcatgga cgagctgtac aagggatctc gcgagggcag
     5341 aggaagtctt ctaacatgcg gtgacgtgga ggagaatccc ggccctatga ccgagtacaa
     5401 gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc
     5461 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg
     5521 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg
     5581 ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg
     5641 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc
     5701 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct
     5761 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct
     5821 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc
     5881 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc
     5941 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgaaatc aacctctgga
     6001 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     6061 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     6121 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     6181 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     6241 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     6301 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     6361 ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac
     6421 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     6481 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtctccgcc ttcgccctca
     6541 gacgagtcgg atctcccttt ggccgcctcc ccgcctggta cctttaagac caatgactta
     6601 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     6661 tcactcccaa cgaaaataag atctgctttt tgcttgtact gggtctctct ggttagacca
     6721 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     6781 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     6841 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct
     6901 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt
     6961 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     7021 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     7081 ctggctctag ctatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     7141 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     7201 aagtagtgag gaggcttttt tggaggccta gacttttgca gagacggccc aaattcgtaa
     7261 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     7321 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     7381 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     7441 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     7501 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     7561 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     7621 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     7681 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     7741 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     7801 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     7861 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     7921 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     7981 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     8041 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     8101 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     8161 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     8221 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     8281 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     8341 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     8401 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     8461 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     8521 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     8581 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     8641 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     8701 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     8761 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     8821 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     8881 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     8941 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     9001 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     9061 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     9121 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     9181 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     9241 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     9301 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     9361 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     9421 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     9481 tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag
     9541 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
     9601 gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg
     9661 agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc
     9721 aggcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct
     9781 tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg
     9841 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgccaagct g
//
