LOCUS       V033760                10345 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033760
VERSION     V033760
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..3018
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK"
                     /label="EGFP"
     CDS             3055..5199
                     /label="CCDC170(NM_025059)"
                     /note="CCDC170(NM_025059)"
                     /gene="CCDC170"
     CDS             5206..5271
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5315..5526
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5539..5807
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5834..6433
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6440..7028
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7102..7335
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7407..7528
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7547..7682
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7720..7736)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7744..7760
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7768..7798)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7813..7834
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8122..8710)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8881..9741)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9742..9846)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10320..10336
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata gaagattcta
     2281 gagctagcga attcagccac catggtgagc aagggcgagg agctgttcac cggggtggtg
     2341 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
     2401 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
     2461 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc
     2521 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     2581 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     2641 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     2701 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
     2761 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
     2821 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     2881 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac
     2941 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     3001 atggacgagc tgtacaaggg aggaggggga agcggtggag gcggttctct cgagatgagc
     3061 ctggactgca ccagccatat cgcgctgggt gccgcttcgc cagcgcccga ggaaacttac
     3121 gatcatcttt cggaagtccc ggtcacgcgg gagcagttaa accactatcg gaatgtggct
     3181 caaaatgctc gaagtgaact tgcagcaact ttggtcaaat ttgaatgtgc tcagtctgag
     3241 cttcaagacc tccgatccaa gatgctttct aaagaagtct cctgtcaaga actgaaagct
     3301 gaaatggaga gctacaagga aaacaatgcc agaaaatcat ctctccttac ctctttgaga
     3361 gacagagttc aggaactaga agaagaatca gcagcacttt ccacttctaa aatcagaaca
     3421 gaaatcacag ctcacgctgc aatcaaggag aaccaggaat taaagaagaa agttgtagag
     3481 ttaaatgaaa aattacaaaa gtgttcaaaa gaaaatgagg agaataagaa acaagtttca
     3541 aagaattgca ggaaacatga ggaatttctg actcaactgc gtgactgctt ggatccagat
     3601 gagaggaatg acaaggcatc agatgaagat ttaattttaa agcttagaga cctgcgcaaa
     3661 gaaaatgaat tcgtgaaagg acaaattgtt attcttgaag agactataaa tgtccatgag
     3721 atggaagcaa aagctagcag agaaacgatc atgaggctgg cttcagaagt caacagagag
     3781 cagaaaaaag ctgcctcctg tactgaagag aaagagaagc tgaaccagga cctgctcagt
     3841 gctgtagaag caaaagaagc tcttgaaagg gaagttaaga tcttccaaga aaggctgctt
     3901 gctggccagc aggtctggga tgcctcaaag caggaagtga gcctcctgaa gaaaagctct
     3961 tctgagttgg agaagagttt gaaggccagt caggatgcag tcacaacctc acaaagccag
     4021 tacttctcat ttagggagaa aatcgcagcc ctccttaggg gcagattgag catgactggg
     4081 tccactgagg acaccatttt ggagaagatt cgagaaatgg acagccggga agaaagcagg
     4141 gaccggatgg tctcccagct tgaagcccaa atatctgagc ttgttgaaca gttgggaaag
     4201 gagtctgggt ttcaccagaa agctctccag agggcccaga aagcagagaa tatgttggag
     4261 actcttcagg gtcagctgac acacctggag gcagagctgg tttctggagg tgttttgcga
     4321 gacaacttga attttgagaa acaaaaatat cttaaatttc tggatcagct ttctcagaaa
     4381 atgaagttgg accagatggc tgccgaactt ggctttgaca tgcggctgga cgtggtttta
     4441 gctcgaacag agcagctggt tcgtcttgag agcaatgcag tcattgagaa caagaccatt
     4501 gcccacaatt tgcagagaaa gctaaagaca cagaaagaga gactggagag caaagaatta
     4561 cacatgagcc tcctccggca gaaaatagcc cagctggagg aggagaagca ggcacgcacg
     4621 gccttggtgg ttgagaggga caacgcgcat cttaccatca ggaacttgca gaagaaggtg
     4681 gagaggctgc agaaagagct gaacacgtgt cgagacttgc acaccgagct caaagccaaa
     4741 ctggccgaca ccaatgaact gaagattaaa actttggaac agactaaagc cattgaagat
     4801 ctaaacaaat ccagagacca actggagaag atgaaggaga aagctgagaa aaagctcatg
     4861 tctgtcaagt cagaactgga taccacagaa catgaggcta aggagaataa agaaagggcc
     4921 agaaacatga tagaagtggt aaccagtgaa atgaagacac taaaaaaatc tctggaagaa
     4981 gcagaaaaga gagaaaagca gctggcagac ttcagggagg tggtgtcgca gatgctaggc
     5041 ttgaacgtga ccagccttgc tcttcctgat tatgaaatca tcaagtgtct tgaaagattg
     5101 gtccattcac atcagcatca ctttgttacc tgtgcctgcc tcaaagatgt gactactggg
     5161 caagagaggc acccacaagg ccatttacag cttcttcatg gatccgacta caaagaccat
     5221 gacggtgatt ataaagatca tgacatcgac tacaaggatg acgatgacaa gtagcggccg
     5281 cgaaggatct gcgatcgctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc
     5341 cccgagaagt tggggggagg ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg
     5401 gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa
     5461 ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga
     5521 acacagctga agcttcgagg ggctcgcatc tctccttcac gcgcccgccg ccctacctga
     5581 ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc ccgcctgtgg tgcctcctga
     5641 actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga gaccgggcct ttgtccggcg
     5701 ctcccttgga gcctacctag actcagccgg ctctccacgc tttgcctgac cctgcttgct
     5761 caactctacg tctttgtttc gttttctgtt ctgcgccgtt acagatccaa gctgtgaccg
     5821 gcgcctacgc tagatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
     5881 cccccgggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
     5941 cgtcgacccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
     6001 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg
     6061 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
     6121 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
     6181 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg
     6241 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
     6301 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
     6361 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
     6421 gcccggtgcc tgagtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg
     6481 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     6541 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     6601 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     6661 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     6721 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     6781 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
     6841 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     6901 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     6961 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     7021 ctccccgcct ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact
     7081 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaaa taagatctgc
     7141 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     7201 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     7261 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     7321 ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa
     7381 agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat
     7441 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     7501 gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac
     7561 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     7621 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     7681 cctagacttt tgcagagacg gcccaaattc gtaatcatgg tcatagctgt ttcctgtgtg
     7741 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     7801 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     7861 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     7921 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     7981 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     8041 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     8101 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     8161 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     8221 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     8281 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     8341 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     8401 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     8461 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     8521 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
     8581 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     8641 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     8701 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     8761 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     8821 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     8881 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     8941 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     9001 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     9061 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     9121 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     9181 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     9241 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     9301 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     9361 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     9421 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     9481 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     9541 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     9601 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     9661 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     9721 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     9781 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     9841 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
     9901 attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga
     9961 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
    10021 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg
    10081 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat
    10141 accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcaggctgcg
    10201 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
    10261 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
    10321 taaaacgacg gccagtgcca agctg
//
