LOCUS       V030728                11726 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V030728
VERSION     V030728
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2291..5752
                     /label="CARD11(NM_001324281)"
                     /note="CARD11(NM_001324281)"
                     /gene="CARD11"
     CDS             5759..5824
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5868..6079
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6092..6360
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      6389..6398
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6419..7081
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             7151..7204
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             7205..7804
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7805..8393
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8467..8700
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8772..8893
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8933..9068
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(9101..9117)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9125..9141
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9149..9179)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9194..9215
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9503..10091)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10262..11122)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11123..11227)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11701..11717
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagccacc atgccaggag gagggccaga gatggatgac tacatggaga cgctgaagga
     2341 tgaagaggac gccttgtggg agaatgtgga gtgtaaccgg cacatgctca gccgctatat
     2401 caaccctgcc aagctcacgc cctacctgcg tcagtgtaag gtcattgatg agcaggatga
     2461 agatgaagtg cttaatgccc ctatgctgcc atccaagatc aaccgagcag gccggctgtt
     2521 ggacattcta cataccaagg ggcaaagggg ctatgtggtc ttcttggaga gcctagaatt
     2581 ttattaccca gaactgtaca aactggtgac tgggaaagag cccactcgga gattctccac
     2641 cattgtggtg gaggaaggcc acgagggcct cacgcacttc ctgatgaacg aggtcatcaa
     2701 gctgcagcag cagatgaagg ccaaggacct gcaacgctgc gagctgctgg ccaggttgcg
     2761 gcagctggag gatgagaaga agcagatgac gctgacgcgc gtggagctgc taaccttcca
     2821 ggagcggtac tacaagatga aggaagagcg ggacagctac aatgacgagc tggtcaaggt
     2881 gaaggacgac aactacaact tagccatgcg ctacgcacag ctcagtgagg agaagaacat
     2941 ggcggtcatg aggagccgag acctccaact cgagatcgat cagctaaagc accggttgaa
     3001 taagatggag gaggaatgta agctggagag aaatcagtct ctaaaactga agaatgacat
     3061 tgaaaatcgg cccaagaagg agcaggttct ggaactggag cgggagaatg aaatgctgaa
     3121 gaccaaaaac caggagctgc agtccatcat ccaggccggg aagcgcagcc tgccagactc
     3181 agacaaggcc atcctggaca tcttggaaca cgaccgcaag gaggccctgg aggacaggca
     3241 ggagctggtc aacaggatct acaacctgca ggaggaggcc cgccaggcag aggagctgcg
     3301 agacaagtac ctggaggaga aggaggacct ggagctcaag tgctcgaccc tgggaaagga
     3361 ctgtgaaatg tacaagcacc gcatgaacac ggtcatgctg cagctggagg aggtggagcg
     3421 ggagcgggac caggccttcc actcccgaga tgaagctcag acacagtact cgcagtgctt
     3481 aatcgaaaag gacaagtaca ggaagcagat ccgcgagctg gaggagaaga acgacgagat
     3541 gaggatcgag atggtgcggc gggaggcctg catcgtcaac ctggagagca agctgcggcg
     3601 cctctccaag gacagcaaca acctggacca gagtctgccc aggaacctgc cagtaaccat
     3661 catctctcag gactttgggg atgccagccc caggaccaat ggtcaagaag ctgacgattc
     3721 ttccacctcg gaggagtcac ctgaagacag caagtacttc ctgccctacc atccgcccca
     3781 gcgcaggatg aacctgaagg gcatccagct gcagagagcc aaatccccca tcagcctgaa
     3841 gcgaacatca gattttcaag ccaaggggca cgaggaagaa ggcacggacg ccagccctag
     3901 ctcctgcgga tctctgccca tcaccaactc cttcaccaag atgcagcccc cccggagccg
     3961 cagcagcatc atgtcaatca ccgccgagcc cccgggaaac gactccatcg tcagacgcta
     4021 caaggaggac gcgccccatc gcagcacagt cgaagaagac aatgacagcg gcgggtttga
     4081 cgccttagat ctggatgatg acagtcacga acgctactcc ttcggaccct cctccatcca
     4141 ctcctcctcc tcctcccacc aatccgaggg cctggatgcc tacgacctgg agcaggtcaa
     4201 cctcatgttc aggaagttct ctctggaaag acccttccgg ccttcggtca cctctgtggg
     4261 gcacgtgcgg ggcccagggc cctcggtgca gcacacgacg ctgaatggcg acagcctcac
     4321 ctcccagctc accctgctgg ggggcaacgc gcgagggagc ttcgtgcact cggtcaagcc
     4381 tggctctctg gccgagaaag ccggcctccg tgagggccac cagctgctgc tgctagaagg
     4441 ctgcatccga ggcgagaggc agagtgtccc gttggacaca tgcaccaaag aggaagccca
     4501 ctggaccatc cagaggtgca gcggccccgt cacgctgcac tacaaggtca accacgaagg
     4561 gtaccggaag ctggtgaagg acatggagga cggcctgatc acatcggggg actcgttcta
     4621 catccggctg aacctgaaca tctccagcca gctggacgcc tgcaccatgt ccctgaagtg
     4681 tgacgatgtt gtgcacgtcc gtgacaccat gtaccaggac aggcacgagt ggctgtgcgc
     4741 gcgggtcgac cctttcacag accatgacct ggatatgggc accataccca gctacagccg
     4801 agcccagcag ctcctcctgg tgaaactgca gcgcctgatg caccgaggca gccgggagga
     4861 ggtagacggc acccaccaca ccctgcgggc actccggaac accctgcagc cagaagaagc
     4921 gctttcaaca agcgaccccc gggtcagccc ccgtctctcg cgagcaagct tcctttttgg
     4981 ccagctcctt cagttcgtca gcaggtccga gaacaagtat aagcggatga acagcaacga
     5041 gcgggtccgc atcatctcgg ggagtccgct agggagcctg gcccggtcct cgctggacgc
     5101 caccaagctc ttgactgaga agcaggaaga gctggaccct gagagcgagc tgggcaagaa
     5161 cctcagcctc atcccctaca gcctggtacg cgccttctac tgcgagcgcc gccggcccgt
     5221 gctcttcaca cccaccgtgc tggccaagac gctggtgcag aggctgctca actcgggagg
     5281 tgccatggag ttcaccatct gcaagtcaga tatcgtcaca agagatgagt tcctcagaag
     5341 gcagaagacg gagaccatca tctactcccg agagaagaac cccaacgcgt tcgaatgcat
     5401 cgcccctgcc aacattgaag ctgtggccgc caagaacaag cactgcctgc tggaggctgg
     5461 gatcggctgc acaagagact tgatcaagtc caacatctac cccatcgtgc tcttcatccg
     5521 ggtgtgtgag aagaacatca agaggttcag aaagctgctg ccccgacctg agacggagga
     5581 ggagttcctg cgcgtgtgcc ggctgaagga gaaggagctg gaggccctgc cgtgcctgta
     5641 cgccacggtg gaacctgaca tgtggggcag cgtagaggag ctgctccgcg ttgtcaagga
     5701 caagatcggc gaggagcagc gcaagaccat ctgggtggac gaggaccagc tgggatccga
     5761 ctacaaagac catgacggtg attataaaga tcatgacatc gactacaagg atgacgatga
     5821 caagtagcgg ccgcgaagga tctgcgatcg ctccggtgcc cgtcagtggg cagagcgcac
     5881 atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaacgg gtgcctagag
     5941 aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga
     6001 gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg
     6061 gtttgccgcc agaacacagc tgaagcttcg aggggctcgc atctctcctt cacgcgcccg
     6121 ccgccctacc tgaggccgcc atccacgccg gttgagtcgc gttctgccgc ctcccgcctg
     6181 tggtgcctcc tgaactgcgt ccgccgtcta ggtaagttta aagctcaggt cgagaccggg
     6241 cctttgtccg gcgctccctt ggagcctacc tagactcagc cggctctcca cgctttgcct
     6301 gaccctgctt gctcaactct acgtctttgt ttcgttttct gttctgcgcc gttacagatc
     6361 caagctgtga ccggcgccta cgctagacgc caccatggag agcgacgaga gcggcctgcc
     6421 cgccatggag atcgagtgcc gcatcaccgg caccctgaac ggcgtggagt tcgagctggt
     6481 gggcggcgga gagggcaccc ccaagcaggg ccgcatgacc aacaagatga agagcaccaa
     6541 aggcgccctg accttcagcc cctacctgct gagccacgtg atgggctacg gcttctacca
     6601 cttcggcacc taccccagcg gctacgagaa ccccttcctg cacgccatca acaacggcgg
     6661 ctacaccaac acccgcatcg agaagtacga ggacggcggc gtgctgcacg tgagcttcag
     6721 ctaccgctac gaggccggcc gcgtgatcgg cgacttcaag gtggtgggca ccggcttccc
     6781 cgaggacagc gtgatcttca ccgacaagat catccgcagc aacgccaccg tggagcacct
     6841 gcaccccatg ggcgataacg tgctggtggg cagcttcgcc cgcaccttca gcctgcgcga
     6901 cggcggctac tacagcttcg tggtggacag ccacatgcac ttcaagagcg ccatccaccc
     6961 cagcatcctg cagaacgggg gccccatgtt cgccttccgc cgcgtggagg agctgcacag
     7021 caacaccgag ctgggcatcg tggagtacca gcacgccttc aagaccccca tcgccttcgc
     7081 cagatcccgc gctcagtcgt ccaattctgc cgtggacggc accgccggac ccggctccac
     7141 cggatctcgc gagggcagag gaagtcttct aacatgcggt gacgtggagg agaatcccgg
     7201 ccctatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc
     7261 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc
     7321 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
     7381 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc
     7441 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag
     7501 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
     7561 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
     7621 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt
     7681 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
     7741 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
     7801 ctgaaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     7861 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     7921 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     7981 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     8041 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     8101 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     8161 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct
     8221 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     8281 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     8341 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggtac
     8401 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     8461 ggggactgga agggctaatt cactcccaac gaagataaga tctgcttttt gcttgtactg
     8521 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     8581 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     8641 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     8701 gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
     8761 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     8821 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     8881 tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc
     8941 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     9001 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     9061 tggaggccta gacttttgca gagaccaaat tcgtaatcat gtcatagctg tttcctgtgt
     9121 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     9181 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     9241 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     9301 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     9361 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     9421 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     9481 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     9541 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     9601 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     9661 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     9721 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     9781 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     9841 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     9901 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
     9961 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
    10021 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
    10081 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
    10141 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
    10201 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
    10261 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
    10321 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
    10381 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
    10441 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
    10501 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
    10561 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
    10621 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
    10681 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
    10741 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
    10801 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
    10861 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
    10921 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
    10981 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
    11041 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
    11101 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
    11161 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
    11221 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
    11281 cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg
    11341 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
    11401 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct
    11461 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa
    11521 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc
    11581 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag
    11641 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt
    11701 gtaaaacgac ggccagtgcc aagctg
//
