LOCUS       V041726                14144 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V041726
VERSION     V041726
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..8976
                     /label="CAD(NM_004341)"
                     /note="CAD(NM_004341)"
                     /gene="CAD"
     CDS             8983..9009
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             9013..9039
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             9043..9069
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     promoter        9114..9325
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             9338..9606
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             9633..10232
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    10239..10827
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             10901..11134
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    11206..11327
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      11346..11481
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(11519..11535)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    11543..11559
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(11567..11597)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    11612..11633
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(11921..12509)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(12680..13540)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(13541..13645)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     14119..14135
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catggcggcc ctagtgttgg aggacgggtc ggtcctgcgg
     2341 ggccagccct ttggggccgc cgtgtcgact gccggggaag tggtgtttca aaccggcatg
     2401 gtcggctacc ccgaggccct cactgatccc tcctacaagg cacagatctt agtgctcacc
     2461 tatcctctga tcggcaacta tggcatcccc ccagatgaaa tggatgagtt cggtctctgc
     2521 aagtggtttg aatcctcggg catccacgta gcagcactgg tagtgggaga gtgctgtcct
     2581 actcccagcc actggagtgc cacccgcacc ctgcatgagt ggctgcagca gcatggcatc
     2641 cctggcttgc aaggagtaga cactcgggag ctgaccaaga agttgcggga acaggggtct
     2701 ctgctgggga agctggtcca gaatggaaca gaaccttcat ccctgccatt cttggacccc
     2761 aatgcccgcc ccctggtacc agaggtctcc attaagactc cacgggtatt caatacaggg
     2821 ggtgcccctc ggatccttgc tttggactgt ggcctcaagt ataatcagat ccgatgcctc
     2881 tgccagcgtg gggctgaggt cactgtggta ccctgggacc atgcactaga cagccaagag
     2941 tatgagggtc tcttcttaag taatgggcct ggtgaccctg cctcctatcc cagtgtcgta
     3001 tccacactga gccgtgtttt atctgagcct aatccccgac ctgtctttgg gatctgcctg
     3061 ggacaccagc tattggcctt agccattggg gccaagactt acaagatgag atatgggaac
     3121 cgaggccata accagccctg cttgttggtg ggctctgggc gctgctttct gacatcccag
     3181 aaccatgggt ttgctgtgga gacagactca ctgccagcag actgggctcc tctcttcacc
     3241 aacgccaatg atggttccaa tgaaggcatt gtgcacaaca gcttgccttt cttcagtgtc
     3301 cagtttcacc cagagcacca agctggccct tcagatatgg aactgctttt cgatatcttt
     3361 ctggaaactg tgaaagaggc cacagctggg aaccctgggg gccagacagt tagagagcgg
     3421 ctgactgagc gcctctgtcc ccctgggatt cccactcccg gctctggact tccaccacca
     3481 cgaaaggttc tgatcctggg ctcagggggc ctctccattg gccaagctgg agaatttgac
     3541 tactcgggct ctcaggcaat taaggccctg aaggaggaaa acatccagac gttgctgatc
     3601 aaccccaata ttgccacagt gcagacctcc caggggctgg ccgacaaggt ctattttctt
     3661 cccataacac ctcattatgt aacccaggtg atacgtaatg aacgccccga tggtgtgtta
     3721 ctgacttttg ggggccagac tgctctgaac tgtggtgtgg agctgaccaa ggccggggtg
     3781 ctggctcggt atggggtccg ggtcctgggc acaccagtgg agaccattga gctgaccgag
     3841 gatcgacggg cctttgctgc cagaatggca gagatcggag agcatgtggc cccgagcgag
     3901 gcagcaaatt ctcttgaaca ggcccaggca gccgctgaac ggctggggta ccctgtgcta
     3961 gtgcgtgcag cctttgccct gggtggcctg ggctctggct ttgcctctaa cagggaggag
     4021 ctctctgctc tcgtggcccc agcttttgcc cataccagcc aagtgctagt agacaagtct
     4081 ctgaagggat ggaaggagat tgagtacgag gtggtgagag acgcctatgg caactgtgtc
     4141 acggtgtgta acatggagaa cttggaccca ctgggcatcc acactggtga gtccatagtg
     4201 gtggccccta gccagacact gaatgacagg gagtatcagc tcctgaggca gacagctatc
     4261 aaggtgaccc agcacctggg aattgttggg gagtgcaatg tgcagtatgc cttgaaccct
     4321 gagtctgagc agtattacat cattgaagtg aatgccaggc tctctcgcag ctctgccctg
     4381 gccagtaagg ccacaggtta tccactggct tatgtggcag ccaagctagc attgggcatc
     4441 cctttgcctg agctcaggaa ctctgtgaca gggggtacag cagcctttga acccagcgtg
     4501 gattattgtg tggtgaagat tcctcgatgg gaccttagca agttcctgcg agtcagcaca
     4561 aagattggga gctgcatgaa gagcgttggt gaagtcatgg gcattgggcg ttcatttgag
     4621 gaggccttcc agaaggccct gcgcatggtg gatgagaact gtgtgggctt tgatcacaca
     4681 gtgaaaccag tcagcgatat ggagttggag actccaacag ataagcggat ttttgtggtg
     4741 gcagctgctt tgtgggctgg ttattcagtg gaccgcctgt atgagctcac acgcatcgac
     4801 cgctggttcc tgcaccgaat gaagcgtatc atcgcacatg cccagctgct agaacaacac
     4861 cgtggacagc ctttgccgcc agacctgctg caacaggcca agtgtcttgg cttctcagac
     4921 aaacagattg cccttgcagt tctgagcaca gagctggctg ttcgcaagct gcgtcaggaa
     4981 ctggggatct gtccagcagt gaaacagatt gacacagttg cagctgagtg gccagcccag
     5041 acaaattacc tatacctaac gtattggggc accacccatg acctcacctt tcgaacacct
     5101 catgtcctag tccttggctc tggcgtctac cgtattggct ctagcgttga atttgactgg
     5161 tgtgctgtag gctgcatcca gcagctccga aagatgggat ataagaccat catggtgaac
     5221 tataacccag agacagtcag caccgactat gacatgtgtg atcgactcta ctttgatgag
     5281 atctcttttg aggtggtgat ggacatctat gagctcgaga accctgaagg tgtgatccta
     5341 tccatgggtg gacagctgcc caacaacatg gccatggcgt tgcatcggca gcagtgccgg
     5401 gtgctgggca cctcccctga agccattgac tcggctgaga accgtttcaa gttttcccgg
     5461 ctccttgaca ccattggtat cagccagcct cagtggaggg agctcagtga cctcgagtct
     5521 gctcgccaat tctgccagac cgtggggtac ccctgtgtgg tgcgcccctc ctatgtgctg
     5581 agcggtgctg ctatgaatgt ggcctacacg gatggagacc tggagcgctt cctgagcagc
     5641 gcagcagccg tctccaaaga gcatcccgtg gtcatctcca agttcatcca ggaggctaag
     5701 gagattgacg tggatgccgt ggcctctgat ggtgtggtgg cagccatcgc catctctgag
     5761 catgtggaga atgcaggtgt gcattcaggt gatgcgacgc tggtgacccc cccacaagat
     5821 atcactgcca aaaccctgga gcggatcaaa gccattgtgc atgctgtggg ccaggagcta
     5881 caggtcacag gacccttcaa tctgcagctc attgccaagg atgaccagct gaaagttatt
     5941 gaatgcaacg tacgtgtctc tcgctccttc cccttcgttt ccaagacact gggtgtggac
     6001 ctagtagcct tggccacgcg ggtcatcatg ggggaagaag tggaacctgt ggggctaatg
     6061 actggttctg gagtcgtggg agtaaaggtg cctcagttct ccttctcccg cttggcgggt
     6121 gctgacgtgg tgttgggtgt ggaaatgacc agtactgggg aggtggccgg ctttggggag
     6181 agccgctgtg aggcatacct caaggccatg ctaagcactg gctttaagat ccccaagaag
     6241 aatatcctgc tgaccattgg cagctataag aacaaaagcg agctgctccc aactgtgcgg
     6301 ctactggaga gcctgggcta cagcctctat gccagtctcg gcacagctga cttctacact
     6361 gagcatggcg tcaaggtaac agctgtggac tggcactttg aggaggctgt ggatggtgag
     6421 tgcccaccac agcggagcat cctggagcag ctagctgaga aaaactttga gctggtgatt
     6481 aacctgtcaa tgcgtggagc tgggggccgg cgtctctctt cctttgtcac caagggctac
     6541 cgcacccgac gcttggccgc tgacttctcc gtgcccctaa tcatcgatat caagtgcacc
     6601 aaactctttg tggaggccct aggccagatc gggccagccc ctcctttgaa ggtgcatgtt
     6661 gactgtatga cctcccaaaa gcttgtgcga ctgccgggat tgattgatgt ccatgtgcac
     6721 ctgcgggaac caggtgggac acataaggag gactttgctt caggcacagc cgctgccctg
     6781 gctgggggta tcaccatggt gtgtgccatg cctaataccc ggccccccat cattgacgcc
     6841 cctgctctgg ccctggccca gaagctggca gaggctggcg cccggtgcga ctttgcgcta
     6901 ttccttgggg cctcgtctga aaatgcagga accttgggca ccgtggccgg gtctgcagcc
     6961 gggctgaagc tttacctcaa tgagaccttc tctgagctgc ggctggacag cgtggtccag
     7021 tggatggagc atttcgagac atggccctcc cacctcccca ttgtggctca cgcagagcag
     7081 caaaccgtgg ctgctgtcct catggtggct cagctcactc agcgctcagt gcacatatgt
     7141 cacgtggcac ggaaggagga gatcctgcta attaaagctg caaaggcacg gggcttgcca
     7201 gtgacctgcg aggtggctcc ccaccacctg ttcctaagcc atgatgacct ggagcgcctg
     7261 gggcctggga agggggaggt ccggcctgag cttggctccc gccaggatgt ggaagccctg
     7321 tgggagaaca tggctgtcat cgactgcttt gcctcagacc atgctcccca taccttggag
     7381 gagaagtgtg ggtccaggcc cccacctggg ttcccagggt tagagaccat gctgccacta
     7441 ctcctgacgg ctgtaagcga gggccggctc agcctggacg acctgctgca gcgattgcac
     7501 cacaatcctc ggcgcatctt tcacctgccc ccgcaggagg acacctatgt ggaggtggat
     7561 ctggagcatg agtggacaat tcccagccac atgcccttct ccaaggccca ctggacacct
     7621 tttgaagggc agaaagtgaa gggcaccgtc cgccgtgtgg tcctgcgagg ggaggttgcc
     7681 tatatcgatg ggcaggttct ggtacccccg ggctatggac aggatgtacg gaagtggcca
     7741 cagggggctg ttcctcagct cccaccctca gcccctgcca ctagtgagat gaccacgaca
     7801 cctgaaagac cccgccgtgg catcccaggg cttcctgatg gccgcttcca tctgccgccc
     7861 cgaatccatc gagcctccga cccaggtttg ccagctgagg agccaaagga gaagtcctct
     7921 cggaaggtag ccgagccaga gctgatggga acccctgatg gcacctgcta ccctccacca
     7981 ccagtaccga gacaggcatc tccccagaac ctggggaccc ctggcttgct gcacccccag
     8041 acctcacccc tgctgcactc attagtgggc caacatatcc tgtccgtcca gcagttcacc
     8101 aaggatcaga tgtctcacct gttcaatgtg gcacacacac tgcgtatgat ggtgcagaag
     8161 gagcggagcc tcgacatcct gaaggggaag gtcatggcct ccatgttcta tgaagtgagc
     8221 acacggacca gcagctcctt tgcagcagcc atggcccggc tgggaggtgc tgtgctcagc
     8281 ttctcggaag ccacatcgtc cgtccagaag ggcgaatccc tggctgactc cgtgcagacc
     8341 atgagctgct atgccgacgt cgtcgtgctc cggcaccccc agcctggagc agtggagctg
     8401 gccgccaagc actgccggag gccagtgatc aatgctgggg atggggtcgg agagcacccc
     8461 acccaggccc tgctggacat cttcaccatc cgtgaggagc tgggaactgt caatggcatg
     8521 acgatcacga tggtgggtga cctgaagcac ggacgcacag tacattccct ggcctgcctg
     8581 ctcacccagt atcgtgtcag cctgcgctac gtggcacctc ccagcctgcg catgccaccc
     8641 actgtgcggg ccttcgtggc ctcccgcggc accaagcagg aggaattcga gagcattgag
     8701 gaggcgctgc ctgacactga tgtgctctac atgactcgaa tccagaagga acgatttggc
     8761 tctacccagg agtacgaagc ttgctttggt cagttcatcc tcactcccca catcatgacc
     8821 cgggccaaga agaagatggt ggtgatgcac ccgatgcccc gtgtcaacga gataagcgtg
     8881 gaagtggact cggatccccg cgcagcctac ttccgccagg ctgagaacgg catgtacatc
     8941 cgcatggctc tgttagccac cgtgctgggc cgtttcggat cctacccata cgacgtacct
     9001 gattatgcaa cttatcctta tgatgtacct gattatgcta gctatcctta tgatgtccca
     9061 gactacgcat aggcggccgc gaaggatctg cgatcgctcc ggtgcccgtc agtgggcaga
     9121 gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaacgggtgc
     9181 ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt
     9241 tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg
     9301 caacgggttt gccgccagaa cacagctgaa gcttcgaggg gctcgcatct ctccttcacg
     9361 cgcccgccgc cctacctgag gccgccatcc acgccggttg agtcgcgttc tgccgcctcc
     9421 cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta agtttaaagc tcaggtcgag
     9481 accgggcctt tgtccggcgc tcccttggag cctacctaga ctcagccggc tctccacgct
     9541 ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg ttttctgttc tgcgccgtta
     9601 cagatccaag ctgtgaccgg cgcctacgct agatgaccga gtacaagccc acggtgcgcc
     9661 tcgccacccg cgacgacgtc ccccgggccg tacgcaccct cgccgccgcg ttcgccgact
     9721 accccgccac gcgccacacc gtcgacccgg accgccacat cgagcgggtc accgagctgc
     9781 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg
     9841 gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg
     9901 agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg
     9961 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg
    10021 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg
    10081 cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct
    10141 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca
    10201 cctggtgcat gacccgcaag cccggtgcct gagtcgacaa tcaacctctg gattacaaaa
    10261 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
    10321 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
    10381 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
    10441 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
    10501 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
    10561 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
    10621 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc
    10681 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
    10741 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
    10801 ggatctccct ttgggccgcc tccccgcctg gtacctttaa gaccaatgac ttacaaggca
    10861 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
    10921 caacgaaaat aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga
    10981 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
    11041 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
    11101 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt
    11161 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca
    11221 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
    11281 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc
    11341 tagctatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
    11401 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
    11461 gaggaggctt ttttggaggc ctagactttt gcagagacgg cccaaattcg taatcatggt
    11521 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
    11581 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
    11641 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
    11701 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
    11761 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
    11821 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
    11881 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
    11941 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
    12001 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
    12061 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
    12121 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
    12181 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
    12241 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
    12301 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
    12361 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
    12421 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
    12481 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
    12541 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
    12601 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
    12661 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
    12721 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
    12781 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
    12841 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
    12901 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
    12961 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
    13021 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
    13081 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
    13141 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
    13201 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
    13261 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
    13321 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
    13381 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
    13441 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
    13501 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
    13561 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
    13621 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag
    13681 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
    13741 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
    13801 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
    13861 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
    13921 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
    13981 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
    14041 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
    14101 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctg
//
