LOCUS       V025989                14109 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V025989
VERSION     V025989
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2301..8963
                     /label="CACNA1C(NM_199460)"
                     /note="CACNA1C(NM_199460)"
                     /gene="CACNA1C"
     CDS             8970..9035
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        9079..9290
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             9303..9571
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             9598..10197
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    10204..10792
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             10866..11099
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    11171..11292
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      11311..11446
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(11484..11500)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    11508..11524
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(11532..11562)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    11577..11598
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(11886..12474)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(12645..13505)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(13506..13610)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     14084..14100
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata gaagattcta
     2281 gagctagcga attcgccacc atggtcaatg agaatacgag gatgtacatt ccagaggaaa
     2341 accaccaagg ttccaactat gggagcccac gccccgccca tgccaacatg aatgccaatg
     2401 cggcagcggg gctggcccct gagcacatcc ccaccccggg ggctgccctg tcgtggcagg
     2461 cggccatcga cgcagcccgg caggctaagc tgatgggcag cgctggcaat gcgaccatct
     2521 ccacagtcag ctccacgcag cggaagcggc agcaatatgg gaaacccaag aagcagggca
     2581 gcaccacggc cacacgcccg ccccgagccc tgctctgcct gaccctgaag aaccccatcc
     2641 ggagggcctg catcagcatt gtcgaatgga aaccatttga aataattatt ttactgacta
     2701 tttttgccaa ttgtgtggcc ttagcgatct atattccctt tccagaagat gattccaacg
     2761 ccaccaattc caacctggaa cgagtggaat atctctttct cataattttt acggtggaag
     2821 cgtttttaaa agtaatcgcc tatggactcc tctttcaccc caatgcctac ctccgcaacg
     2881 gctggaacct actagatttt ataattgtgg ttgtggggct ttttagtgca attttagaac
     2941 aagcaaccaa agcagatggg gcaaacgctc tcggagggaa aggggccgga tttgatgtga
     3001 aggcgctgag ggccttccgc gtgctgcgcc ccctgcggct ggtgtccgga gtcccaagtc
     3061 tccaggtggt cctgaattcc atcatcaagg ccatggtccc cctgctgcac atcgccctgc
     3121 ttgtgctgtt tgtcatcatc atctacgcca tcatcggctt ggagctcttc atggggaaga
     3181 tgcacaagac ctgctacaac caggagggca tagcagatgt tccagcagaa gatgaccctt
     3241 ccccttgtgc gctggaaacg ggccacgggc ggcagtgcca gaacggcacg gtgtgcaagc
     3301 ccggctggga tggtcccaag cacggcatca ccaactttga caactttgcc ttcgccatgc
     3361 tcacggtgtt ccagtgcatc accatggagg gctggacgga cgtgctgtac tgggtcaatg
     3421 atgccgtagg aagggactgg ccctggatct attttgttac actaatcatc atagggtcat
     3481 tttttgtact taacttggtt ctcggtgtgc ttagcggaga gttttccaaa gagagggaga
     3541 aggccaaggc ccggggagat ttccagaagc tgcgggagaa gcagcagcta gaagaggatc
     3601 tcaaaggcta cctggattgg atcactcagg ccgaagacat cgatcctgag aatgaggacg
     3661 aaggcatgga tgaggagaag ccccgaaaca tgagcatgcc caccagtgag accgagtccg
     3721 tcaacaccga aaacgtggct ggaggtgaca tcgagggaga aaactgcggg gccaggctgg
     3781 cccaccggat ctccaagtca aagttcagcc gctactggcg ccggtggaat cggttctgca
     3841 gaaggaagtg ccgcgccgca gtcaagtcta atgtcttcta ctggctggtg attttcctgg
     3901 tgttcctcaa cacgctcacc attgcctctg agcactacaa ccagcccaac tggctcacag
     3961 aagtccaaga cacggcaaac aaggccctgc tggccctgtt cacggcagag atgctcctga
     4021 agatgtacag cctgggcctg caggcctact tcgtgtccct cttcaaccgc tttgactgct
     4081 tcgtcgtgtg tggcggcatc ctggagacca tcctggtgga gaccaagatc atgtccccac
     4141 tgggcatctc cgtgctcaga tgcgtccggc tgctgaggat tttcaagatc acgaggtact
     4201 ggaactcctt gagcaacctg gtggcatcct tgctgaactc tgtgcgctcc atcgcctccc
     4261 tgctccttct cctcttcctc ttcatcatca tcttctccct cctggggatg cagctctttg
     4321 gaggaaagtt caactttgat gagatgcaga cccggaggag cacattcgat aacttccccc
     4381 agtccctcct cactgtgttt cagatcctga ccggggagga ctggaattcg gtgatgtatg
     4441 atgggatcat ggcttatggc ggcccctctt ttccagggat gttagtctgt atttacttca
     4501 tcatcctctt catctgtgga aactatatcc tactgaatgt gttcttggcc attgctgtgg
     4561 acaacctggc tgatgctgag agcctcacat ctgcccaaaa ggaggaggaa gaggagaagg
     4621 agagaaagaa gctggccagg actgccagcc cagagaagaa acaagagttg gtggagaagc
     4681 cggcagtggg ggaatccaag gaggagaaga ttgagctgaa atccatcacg gctgacggag
     4741 agtctccacc cgccaccaag atcaacatgg atgacctcca gcccaatgaa aatgaggata
     4801 agagccccta ccccaaccca gaaactacag gagaagagga tgaggaggag ccagagatgc
     4861 ctgtcggccc tcgcccacga ccactctctg agcttcacct taaggaaaag gcagtgccca
     4921 tgccagaagc cagcgcgttt ttcatcttca gctctaacaa caggtttcgc ctccagtgcc
     4981 accgcattgt caatgacacg atcttcacca acctgatcct cttcttcatt ctgctcagca
     5041 gcatttccct ggctgctgag gacccggtcc agcacacctc cttcaggaac catattctgt
     5101 tttattttga tattgttttt accaccattt tcaccattga aattgctctg aagatcctag
     5161 gcaatgcaga ctatgtcttc actagtatct ttacattaga aattatcctt aagatgactg
     5221 cttatggggc tttcttgcac aagggttctt tctgccggaa ctacttcaac atcctggacc
     5281 tgctggtggt cagcgtgtcc ctcatctcct ttggcatcca gtccagtgca atcaatgtcg
     5341 tgaagatctt gcgagtcctg cgagtactca ggcccctgag ggccatcaac agggccaagg
     5401 ggctaaagca tgtggttcag tgtgtgtttg tcgccatccg gaccatcggg aacatcgtga
     5461 ttgtcaccac cctgctgcag ttcatgtttg cctgcatcgg ggtccagctc ttcaagggaa
     5521 agctgtacac ctgttcagac agttccaagc agacagaggc ggaatgcaag ggcaactaca
     5581 tcacgtacaa agacggggag gttgaccacc ccatcatcca accccgcagc tgggagaaca
     5641 gcaagtttga ctttgacaat gttctggcag ccatgatggc cctcttcacc gtctccacct
     5701 tcgaagggtg gccagagctg ctgtaccgct ccatcgactc ccacacggaa gacaagggcc
     5761 ccatctacaa ctaccgtgtg gagatctcca tcttcttcat catctacatc atcatcatcg
     5821 ccttcttcat gatgaacatc ttcgtgggct tcgtcatcgt cacctttcag gagcaggggg
     5881 agcaggagta caagaactgt gagctggaca agaaccagcg acagtgcgtg gaatacgccc
     5941 tcaaggcccg gcccctgcgg aggtacatcc ccaagaacca gcaccagtac aaagtgtggt
     6001 acgtggtcaa ctccacctac ttcgagtacc tgatgttcgt cctcatcctg ctcaacacca
     6061 tctgcctggc catgcagcac tacggccaga gctgcctgtt caaaatcgcc atgaacatcc
     6121 tcaacatgct cttcactggc ctcttcaccg tggagatgat cctgaagctc attgccttca
     6181 aacccaaggg ttactttagt gatccctgga atgtttttga cttcctcatc gtaattggca
     6241 gcataattga cgtcattctc agtgagacta atcactattt ctgtgatgca tggaatacat
     6301 ttgacgcctt gattgttgtg ggtagcattg ttgatatagc aatcaccgag gtaaacccag
     6361 ctgaacatac ccaatgctct ccctctatga acgcagagga aaactcccgc atctccatca
     6421 ccttcttccg cctgttccgg gtcatgcgtc tggtgaagct gctgagccgt ggggagggca
     6481 tccggacgct gctgtggacc ttcatcaagt ccttccaggc cctgccctat gtggccctcc
     6541 tgatcgtgat gctgttcttc atctacgcgg tgatcgggat gcaggtgttt gggaaaattg
     6601 ccctgaatga taccacagag atcaaccgga acaacaactt tcagaccttc ccccaggccg
     6661 tgctgctcct cttcaggtgt gccaccgggg aggcctggca ggacatcatg ctggcctgca
     6721 tgccaggcaa gaagtgtgcc ccagagtccg agcccagcaa cagcacggag ggtgaaacac
     6781 cctgtggtag cagctttgct gtcttctact tcatcagctt ctacatgctc tgtgccttcc
     6841 tgatcatcaa cctctttgta gctgtcatca tggacaactt tgactacctg acaagggact
     6901 ggtccatcct tggtccccac cacctggatg agtttaaaag aatctgggca gagtatgacc
     6961 ctgaagccaa gggtcgtatc aaacacctgg atgtggtgac cctcctccgg cggattcagc
     7021 cgccactagg ttttgggaag ctgtgccctc accgcgtggc ttgcaaacgc ctggtctcca
     7081 tgaacatgcc tctgaacagc gacgggacag tcatgttcaa tgccaccctg tttgccctgg
     7141 tcaggacggc cctgaggatc aaaacagaag ggaacctaga acaagccaat gaggagctgc
     7201 gggcgatcat caagaagatc tggaagcgga ccagcatgaa gctgctggac caggtggtgc
     7261 cccctgcagg tgatgatgag gtcaccgttg gcaagttcta cgccacgttc ctgatccagg
     7321 agtacttccg gaagttcaag aagcgcaaag agcagggcct tgtgggcaag ccctcccaga
     7381 ggaacgcgct gtctctgcag gctggcttgc gcacactgca tgacatcggg cctgagatcc
     7441 gacgggccat ctctggagat ctcaccgctg aggaggagct ggacaaggcc atgaaggagg
     7501 ctgtgtccgc tgcttctgaa gatgacatct tcaggagggc cggtggcctg ttcggcaacc
     7561 acgtcagcta ctaccaaagc gacggccgga gcgccttccc ccagaccttc accactcagc
     7621 gcccgctgca catcaacaag gcgggcagca gccagggcga cactgagtcg ccatcccacg
     7681 agaagctggt ggactccacc ttcaccccga gcagctactc gtccaccggc tccaacgcca
     7741 acatcaacaa cgccaacaac accgccctgg gtcgcctccc tcgccccgcc ggctacccca
     7801 gcacggtcag cactgtggag ggccacgggc cccccttgtc ccctgccatc cgggtgcagg
     7861 aggtggcgtg gaagctcagc tccaacaggg aaaggcacgt tccgatgtgt gaggatctgg
     7921 agctcaggag ggattcaggc tcagcaggga ctcaggctca ctgccttctg ctcaggagag
     7981 caaacccctc taggtgccac tcccgggaga gccaggcagc catggcgggt caggaggaga
     8041 cgtctcagga tgagacctat gaagtgaaga tgaaccatga cacggaggcc tgcagtgagc
     8101 ccagcctgct ctccacagag atgctctcct accaggatga cgaaaatcgg caactgacgc
     8161 tcccagagga ggacaagagg gacatccggc aatctccgaa gaggggtttc ctccgctctg
     8221 cctcactagg tcgaagggcc tccttccacc tggaatgtct gaagcgacag aaggaccgag
     8281 ggggagacat ctctcagaag acagtcctgc ccttgcatct ggttcatcat caggcattgg
     8341 cagtggcagg cctgagcccc ctcctccaga gaagccattc ccctgcctca ttccctaggc
     8401 cttttgccac cccaccagcc acacctggca gccgaggctg gcccccacag cccgtcccca
     8461 ccctgcggct tgagggggtc gagtccagtg agaaactcaa cagcagcttc ccatccatcc
     8521 actgcggctc ctgggctgag accacccccg gtggcggggg cagcagcgcc gcccggagag
     8581 tccggcccgt ctccctcatg gtgcccagcc aggctggggc cccagggagg cagttccacg
     8641 gcagtgccag cagcctggtg gaagcggtct tgatttcaga aggactgggg cagtttgctc
     8701 aagatcccaa gttcatcgag gtcaccaccc aggagctggc cgacgcctgc gacatgacca
     8761 tagaggagat ggagagcgcg gccgacaaca tcctcagcgg gggcgcccca cagagcccca
     8821 atggcgccct cttacccttt gtgaactgca gggacgcggg gcaggaccga gccgggggcg
     8881 aagaggacgc gggctgtgtg cgcgcgcggg gtcgaccgag tgaggaggag ctccaggaca
     8941 gcagggtcta cgtcagcagc ctgggatccg actacaaaga ccatgacggt gattataaag
     9001 atcatgacat cgactacaag gatgacgatg acaagtagcg gccgcgaagg atctgcgatc
     9061 gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg
     9121 gaggggtcgg caattgaacg ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg
     9181 atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag
     9241 tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag ctgaagcttc
     9301 gaggggctcg catctctcct tcacgcgccc gccgccctac ctgaggccgc catccacgcc
     9361 ggttgagtcg cgttctgccg cctcccgcct gtggtgcctc ctgaactgcg tccgccgtct
     9421 aggtaagttt aaagctcagg tcgagaccgg gcctttgtcc ggcgctccct tggagcctac
     9481 ctagactcag ccggctctcc acgctttgcc tgaccctgct tgctcaactc tacgtctttg
     9541 tttcgttttc tgttctgcgc cgttacagat ccaagctgtg accggcgcct acgctagatg
     9601 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtcccccg ggccgtacgc
     9661 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga cccggaccgc
     9721 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc
     9781 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc
     9841 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc
     9901 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc
     9961 gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc
    10021 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag
    10081 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac
    10141 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgagtc
    10201 gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
    10261 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
    10321 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
    10381 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
    10441 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
    10501 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
    10561 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
    10621 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
    10681 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
    10741 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggtacc
    10801 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
    10861 gggactggaa gggctaattc actcccaacg aaaataagat ctgctttttg cttgtactgg
    10921 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
    10981 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
    11041 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
    11101 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga
    11161 gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
    11221 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
    11281 caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc cagttccgcc
    11341 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
    11401 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctaga cttttgcaga
    11461 gacggcccaa attcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
    11521 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
    11581 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
    11641 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
    11701 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
    11761 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
    11821 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
    11881 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
    11941 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
    12001 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
    12061 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
    12121 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
    12181 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
    12241 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
    12301 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
    12361 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
    12421 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
    12481 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
    12541 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
    12601 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
    12661 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
    12721 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
    12781 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
    12841 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
    12901 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
    12961 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
    13021 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
    13081 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
    13141 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
    13201 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
    13261 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
    13321 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
    13381 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
    13441 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
    13501 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
    13561 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
    13621 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
    13681 aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga
    13741 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa
    13801 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca
    13861 tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta
    13921 aggagaaaat accgcatcag gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg
    13981 cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg
    14041 cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt
    14101 gccaagctg
//
