LOCUS       V024286                10553 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V024286
VERSION     V024286
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4596
                     /label="Brinp1(NM_019967)"
                     /note="Brinp1(NM_019967)"
                     /gene="Brinp1"
     CDS             4603..4668
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4712..4923
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4936..5204
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5233..5242
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5263..5925
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5995..6048
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6049..6648
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6649..7236
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7310..7543
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7615..7736
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7755..7890
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7928..7944)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7952..7968
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7976..8006)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8021..8042
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8330..8918)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9089..9949)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9950..10054)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10528..10544
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatga actggaggtt tgttgagctc
     2341 ctctacttcc tgtttgtatg gggccgtatc tcagtgcagc cctcccgcca ggagccagct
     2401 gggacagacc aacatgtctc caaggaattt gattggctta tttcagacag ggggcctttc
     2461 caccactcca ggagctacct atcctttgtg gaaagacacc ggcaaggatt tacaaccaga
     2521 tataaaatat acagggagtt tgcccgttgg aaggtgagga acacagccat agaaaggaga
     2581 gacctggtcc gtcacccagt gcccctcatg ccggagtttc aaaggagcat ccgtctgctt
     2641 ggcaggagac ccaccactca gcagttcatt gataccatca tcaaaaagta cggcacccac
     2701 ctgctcatct ctgctacatt gggaggagag gaggctttga ccatgtacat ggacaaaagt
     2761 cgcctggacc ggaagtcagg gaatgctacc caaagtgttg aagctttgca ccagcttgca
     2821 tcatcctact ttgtcgaccg tgatggcacc atgagaaggc tccatgagat ccagatttca
     2881 actggagcaa tcaaggtcac agaaacacgc actgggcctc tgggctgtaa cagctatgac
     2941 aatctggact ctgtgagttc tgtccttctg caaagcacgg agagcaaact gcaccttcaa
     3001 ggtcttcaga taatcttccc gcagtatctg caagagaagt ttgttcagtc ggccttgagt
     3061 tacatcatgt gcaatggaga gggagagtat gtgtgtcaga acagccagtg tcgctgccag
     3121 tgtgctgagg agttcccaca gtgcaactgc cccatcaccg acatccagat catggagttt
     3181 acgctggcaa acatggccaa ggcctggact gaagcttata aggacctgga gaattcagat
     3241 gagtttaagt catttatgaa gcgtctccca agcaaccact tcctgacgat tggcagcatc
     3301 caccagcact ggggcaatga ctgggacctg cagagccgtt acaagctcct acagagtgcc
     3361 actgaggctc agaggcagaa aatccagagg accgcccgca agctctttgg cctcagtgtg
     3421 cgctgccgcc acaatcccaa ccaccagctg cctagagaga ggacaattca acagtggctt
     3481 gcaagggtcc agtcacttct ctattgcaat gagaatgggt tctggggaac cttcctggaa
     3541 agccagagga gttgtgtgtg ccatggtagc accacactgt gccagcgccc tatcccatgt
     3601 atcataggtg ggaacaacag ctgtgccatg tgcagcctgg ccaacatctc actctgtggc
     3661 tcctgcaata agggctacaa gctgtataga ggccgctgtg aaccacaaaa cgtggactct
     3721 gagcggagtg agcagttcat cagctttgag accgacctgg acttccagga cttggaactg
     3781 aagtacctgt tgcagaagat ggattcacgc ctctatgtcc atactacctt catcagcaat
     3841 gagattcgcc tggatacatt ttttgaccct cggtggcgca agcgcatgtc cctcactctc
     3901 aaaagcaaca agaaccgcat ggactttatc cacatggtga tcggaatgtc catgcgtatc
     3961 tgccagatgc gcaacagcag cctggaccct atgttctttg tctatgtcaa tcccttcagt
     4021 gggagccatt cagaaggctg gaacatgccc tttggggaat ttggctaccc tcgttgggag
     4081 aagatccgtc tgcaaaacag ccaatgctac aattggactc tcttgctggg caatcggtgg
     4141 aaaacttttt tcgagactgt ccatatctac ctacgtagtc ggactcggct acccacccta
     4201 cgcaatgaga ctggccaggg cccggtggac ctgtcggatc cctccaaaag gcagttctac
     4261 atcaagatct ctgatgtaca ggtgtttggg tacagcctga ggttcaatgc tgacctccta
     4321 cgaagtgccg tgcaacaggt gaatcagtcc tatacacagg gtggtcagtt ctattcctcc
     4381 tcatccgtga tgcttctcat gttggacatt cgggaccgaa tcaaccgcct ggcccctcct
     4441 gtggccccag gaaaacccca gctggacttg ttttcctgta tgctgaagca ccgcctgaag
     4501 ctgaccaaca gtgagattat cagggtgaac catgccttgg acctctacaa cactgagatc
     4561 ctcaaacagt ctgaccagat gacagccaaa ctctgcggat ccgactacaa agaccatgac
     4621 ggtgattata aagatcatga catcgactac aaggatgacg atgacaagta gcggccgcga
     4681 aggatctgcg atcgctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc
     4741 gagaagttgg ggggaggggt cggcaattga acgggtgcct agagaaggtg gcgcggggta
     4801 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg
     4861 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca
     4921 cagctgaagc ttcgaggggc tcgcatctct ccttcacgcg cccgccgccc tacctgaggc
     4981 cgccatccac gccggttgag tcgcgttctg ccgcctcccg cctgtggtgc ctcctgaact
     5041 gcgtccgccg tctaggtaag tttaaagctc aggtcgagac cgggcctttg tccggcgctc
     5101 ccttggagcc tacctagact cagccggctc tccacgcttt gcctgaccct gcttgctcaa
     5161 ctctacgtct ttgtttcgtt ttctgttctg cgccgttaca gatccaagct gtgaccggcg
     5221 cctacgctag acgccaccat ggagagcgac gagagcggcc tgcccgccat ggagatcgag
     5281 tgccgcatca ccggcaccct gaacggcgtg gagttcgagc tggtgggcgg cggagagggc
     5341 acccccaagc agggccgcat gaccaacaag atgaagagca ccaaaggcgc cctgaccttc
     5401 agcccctacc tgctgagcca cgtgatgggc tacggcttct accacttcgg cacctacccc
     5461 agcggctacg agaacccctt cctgcacgcc atcaacaacg gcggctacac caacacccgc
     5521 atcgagaagt acgaggacgg cggcgtgctg cacgtgagct tcagctaccg ctacgaggcc
     5581 ggccgcgtga tcggcgactt caaggtggtg ggcaccggct tccccgagga cagcgtgatc
     5641 ttcaccgaca agatcatccg cagcaacgcc accgtggagc acctgcaccc catgggcgat
     5701 aacgtgctgg tgggcagctt cgcccgcacc ttcagcctgc gcgacggcgg ctactacagc
     5761 ttcgtggtgg acagccacat gcacttcaag agcgccatcc accccagcat cctgcagaac
     5821 gggggcccca tgttcgcctt ccgccgcgtg gaggagctgc acagcaacac cgagctgggc
     5881 atcgtggagt accagcacgc cttcaagacc cccatcgcct tcgccagatc ccgcgctcag
     5941 tcgtccaatt ctgccgtgga cggcaccgcc ggacccggct ccaccggatc tcgcgagggc
     6001 agaggaagtc ttctaacatg cggtgacgtg gaggagaatc ccggccctat gaccgagtac
     6061 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc
     6121 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag
     6181 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg
     6241 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg
     6301 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc
     6361 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc
     6421 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg
     6481 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg
     6541 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg
     6601 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgaaa tcaacctctg
     6661 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     6721 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     6781 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     6841 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     6901 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     6961 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     7021 aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc
     7081 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     7141 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtctccg ccttcgccct
     7201 cagacgagtc ggatctccct ttggccgcct ccccgcctgg tacctttaag accaatgact
     7261 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
     7321 attcactccc aacgaaaata agatctgctt tttgcttgta ctgggtctct ctggttagac
     7381 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     7441 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     7501 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat
     7561 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg
     7621 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     7681 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     7741 gtctggctct agctatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     7801 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     7861 agaagtagtg aggaggcttt tttggaggcc tagacttttg cagagacggc ccaaattcgt
     7921 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     7981 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     8041 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     8101 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     8161 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     8221 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     8281 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     8341 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     8401 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     8461 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     8521 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     8581 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     8641 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     8701 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     8761 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     8821 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     8881 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     8941 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     9001 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     9061 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     9121 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     9181 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     9241 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     9301 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     9361 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     9421 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     9481 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     9541 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     9601 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     9661 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     9721 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     9781 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     9841 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     9901 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     9961 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
    10021 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
    10081 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
    10141 cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
    10201 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
    10261 cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac
    10321 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
    10381 tcaggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
    10441 cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa
    10501 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgccaag ctg
//
