LOCUS V025050 8344 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V025050 VERSION V025050 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1804..1920 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2010..2213 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2302..3369 /label="PCBP1(NM_006196)" /note="PCBP1(NM_006196)" /gene="PCBP1" CDS 3376..3465 /codon_start=1 /product="3 tandem Myc epitope tags" /transl_table=1 /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL" /label="3xMyc" promoter 3509..3720 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 3733..4001 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" CDS 4034..4432 /codon_start=1 /gene="Aspergillus terreus BSD" /note="confers resistance to blasticidin" /product="blasticidin S deaminase" /transl_table=1 /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI KAIVKDSDGQPTAVGIRELLPSGYVWEG*" /label="BSD" misc_feature 4439..5027 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 5101..5334 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 5406..5527 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 5546..5681 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(5719..5735) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 5743..5759 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(5767..5797) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 5812..5833 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(6121..6709) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(6880..7740) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(7741..7845) /gene="bla" /label="AmpR promoter" primer_bind 8319..8335 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag 2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct 2281 agagctagcg aattcgccac catggatgcc ggtgtgactg aaagtggact aaatgtgact 2341 ctcaccattc ggcttcttat gcacggaaag gaagtaggaa gcatcattgg gaagaaaggg 2401 gagtcggtta agaggatccg cgaggagagt ggcgcgcgga tcaacatctc ggaggggaat 2461 tgtccggaga gaatcatcac tctgaccggc cccaccaatg ccatctttaa ggctttcgct 2521 atgatcatcg acaagctgga ggaagatatc aacagctcca tgaccaacag taccgcggcc 2581 agcaggcccc cggtcaccct gaggctggtg gtgccggcca cccagtgcgg ctccctgatt 2641 gggaaaggcg ggtgtaagat caaagagatc cgcgagagta cgggggcgca ggtccaggtg 2701 gcgggggata tgctgcccaa ctccaccgag cgggccatca ccatcgctgg cgtgccgcag 2761 tctgtcaccg agtgtgtcaa gcagatttgc ctggtcatgc tggagacgct ctcccagtct 2821 ccgcaaggga gagtcatgac cattccgtac cagcccatgc cggccagctc cccagtcatc 2881 tgcgcgggcg gccaagatcg gtgcagcgac gctgcgggct acccccatgc cacccatgac 2941 ctggagggac cacctctaga tgcctactcg attcaaggac aacacaccat ttctccgctc 3001 gatctggcca agctgaacca ggtggcaaga caacagtctc actttgccat gatgcacggc 3061 gggaccggat tcgccggaat tgactccagc tctccagagg tgaaaggcta ttgggcaagt 3121 ttggatgcat ctactcaaac cacccatgaa ctcaccattc caaataactt aattggctgc 3181 ataatcgggc gccaaggcgc caacattaat gagatccgcc agatgtccgg ggcccagatc 3241 aaaattgcca acccagtgga aggctcctct ggtaggcagg ttactatcac tggctctgct 3301 gccagtatta gtctggccca gtatctaatc aatgccaggc tttcctctga gaagggcatg 3361 gggtgcagcg gatccgaaca aaaactaata tcagaggaag atttggaaca gaaactaatc 3421 agtgaagaag atttagagca aaagttaatt tccgaggagg acttatagcg gccgcgaagg 3481 atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag 3541 aagttggggg gaggggtcgg caattgaacg ggtgcctaga gaaggtggcg cggggtaaac 3601 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat 3661 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag 3721 ctgaagcttc gaggggctcg catctctcct tcacgcgccc gccgccctac ctgaggccgc 3781 catccacgcc ggttgagtcg cgttctgccg cctcccgcct gtggtgcctc ctgaactgcg 3841 tccgccgtct aggtaagttt aaagctcagg tcgagaccgg gcctttgtcc ggcgctccct 3901 tggagcctac ctagactcag ccggctctcc acgctttgcc tgaccctgct tgctcaactc 3961 tacgtctttg tttcgttttc tgttctgcgc cgttacagat ccaagctgtg accggcgcct 4021 acgctaggcc accatggcca agcctttgtc tcaagaagaa tccaccctca ttgaaagagc 4081 aacggctaca atcaacagca tccccatctc tgaagactac agcgtcgcca gcgcagctct 4141 ctctagcgac ggccgcatct tcactggtgt caatgtatat cattttactg ggggaccttg 4201 tgcagaactc gtggtgctgg gcactgctgc tgctgcggca gctggcaacc tgacttgtat 4261 cgtcgcgatc ggaaatgaga acaggggcat cttgagcccc tgcggacggt gccgacaggt 4321 gcttctcgat ctgcatcctg ggatcaaagc catagtgaag gacagtgatg gacagccgac 4381 ggcagttggg attcgtgaat tgctgccctc tggttatgtg tgggagggct aagtcgacaa 4441 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc 4501 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat 4561 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg 4621 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg 4681 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat 4741 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt 4801 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc 4861 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa 4921 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg 4981 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg gtacctttaa 5041 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac 5101 tggaagggct aattcactcc caacgaaaat aagatctgct ttttgcttgt actgggtctc 5161 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 5221 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 5281 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta 5341 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg 5401 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt 5461 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg 5521 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccagtt ccgcccattc 5581 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc 5641 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagactttt gcagagacgg 5701 cccaaattcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat 5761 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag 5821 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg 5881 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc 5941 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc 6001 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa 6061 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt 6121 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg 6181 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg 6241 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag 6301 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc 6361 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa 6421 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg 6481 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc 6541 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac 6601 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg 6661 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt 6721 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt 6781 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa 6841 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga 6901 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt 6961 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg 7021 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga 7081 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga 7141 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg 7201 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc 7261 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc 7321 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca 7381 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac 7441 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg 7501 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc 7561 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg 7621 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac 7681 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat 7741 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata 7801 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa 7861 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg 7921 tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat 7981 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg 8041 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga 8101 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag 8161 aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc 8221 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt 8281 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa 8341 gctg //