LOCUS       V040072                 9719 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V040072
VERSION     V040072
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..4740
                     /label="Kat2b(NM_020005)"
                     /note="Kat2b(NM_020005)"
                     /gene="Kat2b"
     CDS             4747..4773
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4777..4803
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4807..4833
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     promoter        4884..5095
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5108..5376
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5409..5807
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /note="confers resistance to blasticidin"
                     /product="blasticidin S deaminase"
                     /transl_table=1
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG*"
                     /label="BSD"
     misc_feature    5814..6402
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6476..6709
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    6781..6902
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      6921..7056
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7094..7110)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7118..7134
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7142..7172)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7187..7208
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7496..8084)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8255..9115)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9116..9220)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     9694..9710
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catggccgag gctggcgggg ccgggtcccc agcactgcct
     2341 cccgcgcccc cgcacggttc cccccggacc ctggccaccg ctgccgggag ctctgcttcc
     2401 tgcgggccag cgaccgcggt ggccgcggcg ggcacggccg agggaccggg aggaggcggc
     2461 tcggcccgga tcgccgtgaa gaaggcgcag ttgcgctctg ctccgcgggc gaagaagctg
     2521 gagaaactcg gcgtgtactc cgcctgcaag gcagaggagt cctgtaaatg caatggctgg
     2581 aagaacccta acccctctcc tactccacca agaggagacc tccagcagat aattgtcagt
     2641 ttgacagaat cctgtcgaag ctgtagccat gcccttgctg ctcacgtttc tcacttggag
     2701 aatgtgtcag aggaagagat ggacagactc ctgggaattg tgttggatgt ggagtacctc
     2761 ttcacctgcg tccacaaaga agaagatgca gataccaaac aagtgtactt ctacctattc
     2821 aagctcttga gaaagtcaat tttacaaaga ggaaaacctg tggttgaagg ctccttggag
     2881 aagaagccgc catttgagaa gcccagtatt gaacagggtg tgaacaactt cgtgcagtac
     2941 aagtttagtc acttgccatc gaaagagagg cagacaacga tcgagctggc caagatgttt
     3001 ctgaaccgca tcaactactg gcatctggag gctccatctc agcggagact acggtctccc
     3061 aatgatgaca tctctggata caaggaaaac tacacaaggt ggttgtgcta ctgcaatgta
     3121 ccgcagttct gtgacagctt acctcggtac gaaaccacaa aggtgtttgg gagaacattg
     3181 cttcgctcgg tcttcaccat catgagacga cagctcttgg aacaagccag acaggaaaaa
     3241 gacaaactgc ctcttgagaa acgcacgctt atcctcacac atttcccaaa gtttctgtcc
     3301 atgttggaag aagaagtgta tagtcaaaat tctcctatct gggatcagga ttttctctca
     3361 gcctcttcca gaaccagccc gctaggaatc caaacagtaa tcagtcctcc tgttactggg
     3421 acagcattgt tcagttcaaa ttccacttcc catgagcaga tcaatggagg gagaacaagt
     3481 cctggatgca gaggctcttc tgggcttgaa gcaaacccgg gagaaaagag gaaaatgaac
     3541 aactctcatg ctcccgagga ggccaagaga tctcgagtga tgggggatat tcccgtggaa
     3601 ttgatcaatg aggtcatgtc taccatcaca gaccctgcag ggatgcttgg accagagacc
     3661 aattttctgt cagcccattc ggccagagat gaggcggcac ggctggaaga acgcaggggt
     3721 gtcattgaat tccacgtggt gggcaactcc ctgaaccaga aaccaaacaa gaagatcctg
     3781 atgtggctcg tgggcctcca gaatgtgttt tcccaccagc tgcccagaat gcccaaagag
     3841 tacatcacac ggctcgtctt tgacccgaaa cacaaaaccc ttgctttaat taaagatggc
     3901 cgtgtcattg gtggtatctg tttccggatg tttccatccc agggattcac agagattgtt
     3961 ttctgtgcag taacctcaaa tgaacaagtc aagggctatg gaacccacct gatgaaccat
     4021 ctcaaagaat accacataaa gcacgagatc ctcaacttcc tcacatatgc agatgagtat
     4081 gccatcggct atttcaagaa gcagggtttc tccaaagaaa tcaaaatacc taaaaccaaa
     4141 tatgttggct acatcaagga ttatgaaggg gccactttga tgggatgtga gctgaaccct
     4201 cagatcccat acacagagtt ctctgtcatc attaaaaagc agaaggagat cattaaaaag
     4261 ctgatagaaa gaaaacaagc ccagattcga aaagtctacc ctggactttc gtgtttcaaa
     4321 gatggagttc ggcagattcc tatagaaagc attcctggaa tcagagagac aggctggaaa
     4381 ccaagtggaa aagagaaaag taaagagccc aaagaccctg agcagcttta cagcaccctc
     4441 aagaacatcc tgcagcaggt gaagaaccat ccaaatgctt ggcctttcat ggaaccagtg
     4501 aagagaacag aagctccggg atattatgaa gttataaggt tccccatgga tctgaaaacc
     4561 atgagtgaac gcctcaggaa caggtactat gtgtctaaga agttattcat ggcggacttg
     4621 caacgagtgt tcaccaactg caaggagtac aaccctcccg agagcgagta ctacaaatgc
     4681 gccagcatcc tggagaagtt cttcttcagt aaaattaagg aagcagggtt gattgacaag
     4741 ctcgagtacc catacgacgt acctgattat gcaacttatc cttatgatgt acctgattat
     4801 gctagctatc cttatgatgt cccagactac gcatagggat ccgcggccgc gaaggatctg
     4861 cgatcgctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt
     4921 ggggggaggg gtcggcaatt gaacgggtgc ctagagaagg tggcgcgggg taaactggga
     4981 aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag
     5041 tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacagctgaa
     5101 gcttcgaggg gctcgcatct ctccttcacg cgcccgccgc cctacctgag gccgccatcc
     5161 acgccggttg agtcgcgttc tgccgcctcc cgcctgtggt gcctcctgaa ctgcgtccgc
     5221 cgtctaggta agtttaaagc tcaggtcgag accgggcctt tgtccggcgc tcccttggag
     5281 cctacctaga ctcagccggc tctccacgct ttgcctgacc ctgcttgctc aactctacgt
     5341 ctttgtttcg ttttctgttc tgcgccgtta cagatccaag ctgtgaccgg cgcctacgct
     5401 aggccaccat ggccaagcct ttgtctcaag aagaatccac cctcattgaa agagcaacgg
     5461 ctacaatcaa cagcatcccc atctctgaag actacagcgt cgccagcgca gctctctcta
     5521 gcgacggccg catcttcact ggtgtcaatg tatatcattt tactggggga ccttgtgcag
     5581 aactcgtggt gctgggcact gctgctgctg cggcagctgg caacctgact tgtatcgtcg
     5641 cgatcggaaa tgagaacagg ggcatcttga gcccctgcgg acggtgccga caggtgcttc
     5701 tcgatctgca tcctgggatc aaagccatag tgaaggacag tgatggacag ccgacggcag
     5761 ttgggattcg tgaattgctg ccctctggtt atgtgtggga gggctaagtc gacaatcaac
     5821 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     5881 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     5941 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     6001 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     6061 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     6121 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     6181 ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg
     6241 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     6301 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     6361 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggtacc tttaagacca
     6421 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
     6481 gggctaattc actcccaacg aaaataagat ctgctttttg cttgtactgg gtctctctgg
     6541 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     6601 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     6661 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca
     6721 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg
     6781 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     6841 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     6901 tatcatgtct ggctctagct atcccgcccc taactccgcc cagttccgcc cattctccgc
     6961 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     7021 tattccagaa gtagtgagga ggcttttttg gaggcctaga cttttgcaga gacggcccaa
     7081 attcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     7141 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     7201 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     7261 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     7321 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     7381 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     7441 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     7501 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     7561 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     7621 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     7681 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     7741 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     7801 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     7861 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     7921 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     7981 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     8041 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     8101 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     8161 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     8221 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     8281 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     8341 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     8401 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     8461 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     8521 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     8581 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     8641 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     8701 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     8761 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     8821 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     8881 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     8941 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     9001 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     9061 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     9121 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     9181 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     9241 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
     9301 cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
     9361 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
     9421 gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga
     9481 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat
     9541 accgcatcag gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
     9601 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
     9661 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gccaagctg
//
