LOCUS       V033089                 9809 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033089
VERSION     V033089
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..4830
                     /label="FBXO11(NM_025133)"
                     /note="FBXO11(NM_025133)"
                     /gene="FBXO11"
     CDS             4837..4863
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4867..4893
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4897..4923
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     promoter        4974..5185
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5198..5466
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5499..5897
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /note="confers resistance to blasticidin"
                     /product="blasticidin S deaminase"
                     /transl_table=1
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG*"
                     /label="BSD"
     misc_feature    5904..6492
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6566..6799
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    6871..6992
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7011..7146
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7184..7200)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7208..7224
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7232..7262)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7277..7298
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7586..8174)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8345..9205)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9206..9310)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     9784..9800
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catggttgca gaagaatcag gtcctggtgc acaaaatagt
     2341 ccataccaac ttcgtagaaa aactcttttg ccgaaaagaa cagcgtgtcc cacaaagaac
     2401 agtatggagg gcgcctcaac ttcaactaca gaaaactttg gtcatcgtgc aaaacgtgca
     2461 agagtgtctg gaaaatcaca agatctatca gcagcacctg ctgaacagta tcttcaggag
     2521 aaactgccag atgaagtggt tctaaaaatc ttctcttact tgctggaaca ggatctttgt
     2581 agagcagctt gtgtatgtaa acgcttcagt gaacttgcta atgatccaat tttgtggaaa
     2641 cgattatata tggaagtatt tgaatatact cgccctatga tgcatcctga acctggaaaa
     2701 ttctaccaga ttaatccaga agagtatgaa catccaaatc cctggaaaga gagtttccag
     2761 cagttgtata aaggtgcaca tgtaaagcca ggatttgctg aacatttcta cagtaaccct
     2821 gcaagatata aaggaagaga aaatatgttg tattatgata ctattgaaga tgcccttggt
     2881 ggggtacaag aggctcattt tgatggactt atctttgttc attctggaat atatactgat
     2941 gaatggatat atattgaatc tccaatcacc atgattggtg cagcacctgg gaaagtggca
     3001 gacaaagtta taattgaaaa cactagagat tcaaccttcg tttttatgga aggctctgaa
     3061 gatgcttatg ttggatatat gacaataagg tttaaccctg atgacaaatc tgcacaacac
     3121 cacaatgcac accactgctt agagattaca gtaaattgta gccctattat tgatcactgt
     3181 atcatccgaa gtacatgtac agttggttct gcagtatgtg ttagtggtca aggagcatgt
     3241 cccaccatca agcactgtaa catcagtgac tgtgaaaatg ttggactata tataacagat
     3301 catgcacagg gaatatatga ggataatgaa atttccaata atgcgttagc tgggatttgg
     3361 gttaaaaatc atggaaaccc aattattaga cggaatcata ttcatcatgg acgtgatgtt
     3421 ggtgtgttca catttgatca tggcatgggt tactttgaaa gttgcaatat acacagaaat
     3481 aggatagcag gctttgaagt aaaagcctat gctaacccta cagtggttcg atgtgaaatt
     3541 caccatgggc agactggagg aatatatgtc catgaaaaag gaagaggaca attcatagag
     3601 aataaaatct atgcaaacaa ctttgcaggt gtatggatta cctcaaatag tgacccaaca
     3661 ataaggggaa attctatatt taatggaaat caaggaggag tttacatctt tggtgatgga
     3721 cgaggcctta ttgaaggaaa tgacatttat ggcaatgcat tagcaggaat tcaaattagg
     3781 acaaacagtt gtccaattgt tcggcataac aaaattcatg atggccagca tggtgggatt
     3841 tatgtgcatg aaaagggaca aggagtaata gaagagaatg aagtttatag taacactcta
     3901 gctggagtct gggtgacaac tggcagcact ccagtactga gaagaaaccg gatacacagt
     3961 ggcaagcagg ttggtgttta tttttatgac aatggacatg gagtgctaga agacaatgat
     4021 atctataatc atatgtattc aggggttcag ataaggactg gaagcaaccc caaaattaga
     4081 cgcaacaaaa tctggggagg acagaatggt ggaattctag tttataattc tggtctaggc
     4141 tgtatagaag acaatgaaat atttgacaat gcaatggctg gagtctggat taagacagat
     4201 agtaatccta cactaagaag aaataaaatc catgatggaa gagatggtgg catctgtata
     4261 tttaatgggg gtcgaggtct ccttgaagaa aatgatattt tcaggaatgc tcaagcaggt
     4321 gttctcatca gcactaatag tcatccaatc ttaaggaaaa acagaatatt tgatggattt
     4381 gccgcaggta ttgaaattac aaatcacgca actgcaacac tagaaggcaa tcagattttt
     4441 aacaaccggt ttggaggctt atttttagca tctggtgtta atgtgacaat gaaagataac
     4501 aaaataatga acaatcaaga tgccatagaa aaggctgtta gtagaggcca atgtttatat
     4561 aaaatatcaa gttataccag ctatcccatg catgatttct acagatgtca tacttgtaac
     4621 accacagatc gaaatgccat atgtgtgaac tgcattaaga agtgccatca gggacatgat
     4681 gtagagttta ttagacatga taggtttttc tgtgactgtg gtgctggaac actgtctaat
     4741 ccttgtacat tagctggtga gcctacacat gatacagata cactatatga ctctgctcca
     4801 cctatagaat ctaatacatt gcagcacaac ctcgagtacc catacgacgt acctgattat
     4861 gcaacttatc cttatgatgt acctgattat gctagctatc cttatgatgt cccagactac
     4921 gcatagggat ccgcggccgc gaaggatctg cgatcgctcc ggtgcccgtc agtgggcaga
     4981 gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaacgggtgc
     5041 ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt
     5101 tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg
     5161 caacgggttt gccgccagaa cacagctgaa gcttcgaggg gctcgcatct ctccttcacg
     5221 cgcccgccgc cctacctgag gccgccatcc acgccggttg agtcgcgttc tgccgcctcc
     5281 cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta agtttaaagc tcaggtcgag
     5341 accgggcctt tgtccggcgc tcccttggag cctacctaga ctcagccggc tctccacgct
     5401 ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg ttttctgttc tgcgccgtta
     5461 cagatccaag ctgtgaccgg cgcctacgct aggccaccat ggccaagcct ttgtctcaag
     5521 aagaatccac cctcattgaa agagcaacgg ctacaatcaa cagcatcccc atctctgaag
     5581 actacagcgt cgccagcgca gctctctcta gcgacggccg catcttcact ggtgtcaatg
     5641 tatatcattt tactggggga ccttgtgcag aactcgtggt gctgggcact gctgctgctg
     5701 cggcagctgg caacctgact tgtatcgtcg cgatcggaaa tgagaacagg ggcatcttga
     5761 gcccctgcgg acggtgccga caggtgcttc tcgatctgca tcctgggatc aaagccatag
     5821 tgaaggacag tgatggacag ccgacggcag ttgggattcg tgaattgctg ccctctggtt
     5881 atgtgtggga gggctaagtc gacaatcaac ctctggatta caaaatttgt gaaagattga
     5941 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     6001 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     6061 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     6121 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     6181 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     6241 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat
     6301 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     6361 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     6421 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     6481 ccgcctcccc gcctggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
     6541 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aaaataagat
     6601 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     6661 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     6721 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     6781 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt
     6841 gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac
     6901 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     6961 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc
     7021 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     7081 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     7141 gaggcctaga cttttgcaga gacggcccaa attcgtaatc atggtcatag ctgtttcctg
     7201 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     7261 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     7321 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     7381 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     7441 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     7501 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     7561 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     7621 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     7681 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     7741 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     7801 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     7861 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     7921 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     7981 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     8041 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     8101 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     8161 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     8221 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     8281 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     8341 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     8401 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     8461 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     8521 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     8581 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     8641 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     8701 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     8761 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     8821 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     8881 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     8941 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     9001 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     9061 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     9121 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     9181 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     9241 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     9301 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
     9361 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg
     9421 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
     9481 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
     9541 gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
     9601 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc
     9661 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
     9721 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
     9781 gttgtaaaac gacggccagt gccaagctg
//
