LOCUS V046396 8413 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V046396 VERSION V046396 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1804..1920 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2010..2213 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2302..3462 /label="Egr3(NM_018781)" /note="Egr3(NM_018781)" /gene="Egr3" CDS 3469..3534 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" promoter 3578..3789 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 3802..4070 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" CDS 4103..4501 /codon_start=1 /gene="Aspergillus terreus BSD" /note="confers resistance to blasticidin" /product="blasticidin S deaminase" /transl_table=1 /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI KAIVKDSDGQPTAVGIRELLPSGYVWEG*" /label="BSD" misc_feature 4508..5096 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 5170..5403 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 5475..5596 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 5615..5750 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(5788..5804) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 5812..5828 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(5836..5866) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 5881..5902 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(6190..6778) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(6949..7809) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(7810..7914) /gene="bla" /label="AmpR promoter" primer_bind 8388..8404 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag 2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct 2281 agagctagcg aattcgccac catgaccggc aaactcgccg agaagctgcc ggtgaccatg 2341 agcagtttgc taaatcaatt gcctgacaat ctgtaccccg aggagatccc cagcgcgctc 2401 aacctcttct ccggcagcag cgactcggta gcccattaca atcagatggc tacagagaat 2461 gtgatggaca tcggtctgac caacgagaag cccaatccgg aactctctta ttcgagctct 2521 ttccagccag cccccggcaa caagaccgtg acctacttgg gaaagttcgc tttcgactct 2581 ccttccaact ggtgccagga caacatcatt agcctcatga gtgccggcat cttgggggtg 2641 cccccggcct caggggcgct cagtacgcag acgtccacgg ctagcatggt gcagcctcct 2701 cagggagacg tggaggccat gtatccggcg ctgcctcctt attccaactg cggcgatctc 2761 tactcggagc ccgtgtcttt ccacgacccc cagggaaacc ctgggctcgc ctattccccc 2821 caggattacc aatcggccaa gccggccttg gacagcaatc ttttccccat gattcctgac 2881 tacaacctgt accaccatcc caacgacatg ggctccattc cggaacataa gcccttccag 2941 ggcatggacc ccatccgggt caacccaccc cctattaccc cactggagac catcaaggcc 3001 ttcaaagaca agcagatcca cccgggattc ggcagcctgc cccaaccgcc gctcactctc 3061 aagcccatcc ggccccgcaa gtaccccaat cggcctagca agaccccgct ccacgagcgg 3121 ccccacgcgt gtcccgcgga aggctgtgac cgccgtttca gccgctcaga cgagctgacc 3181 cggcacctgc gcatccacac gggccacaag cccttccagt gtcggatctg catgcgcagc 3241 ttcagccgca gcgaccacct caccactcac atccgcacgc acaccgggga gaagcccttt 3301 gcctgtgagt tctgtgggcg caagtttgcg cgcagcgacg agcgcaagcg ccacgccaag 3361 atccacctca agcaaaagga gaagaagtcg gagaaagggg gtgcgccctc tgcatcctcg 3421 gcgcccaccg tgtccctggc ccctgtggtc accacctgcg ccggatccga ctacaaagac 3481 catgacggtg attataaaga tcatgacatc gactacaagg atgacgatga caagtagcgg 3541 ccgcgaagga tctgcgatcg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca 3601 gtccccgaga agttgggggg aggggtcggc aattgaacgg gtgcctagag aaggtggcgc 3661 ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga 3721 gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc 3781 agaacacagc tgaagcttcg aggggctcgc atctctcctt cacgcgcccg ccgccctacc 3841 tgaggccgcc atccacgccg gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc 3901 tgaactgcgt ccgccgtcta ggtaagttta aagctcaggt cgagaccggg cctttgtccg 3961 gcgctccctt ggagcctacc tagactcagc cggctctcca cgctttgcct gaccctgctt 4021 gctcaactct acgtctttgt ttcgttttct gttctgcgcc gttacagatc caagctgtga 4081 ccggcgccta cgctaggcca ccatggccaa gcctttgtct caagaagaat ccaccctcat 4141 tgaaagagca acggctacaa tcaacagcat ccccatctct gaagactaca gcgtcgccag 4201 cgcagctctc tctagcgacg gccgcatctt cactggtgtc aatgtatatc attttactgg 4261 gggaccttgt gcagaactcg tggtgctggg cactgctgct gctgcggcag ctggcaacct 4321 gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc ttgagcccct gcggacggtg 4381 ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc atagtgaagg acagtgatgg 4441 acagccgacg gcagttggga ttcgtgaatt gctgccctct ggttatgtgt gggagggcta 4501 agtcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta 4561 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc 4621 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga 4681 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac 4741 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc 4801 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc 4861 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg 4921 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc 4981 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc 5041 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg 5101 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa 5161 aggggggact ggaagggcta attcactccc aacgaaaata agatctgctt tttgcttgta 5221 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc 5281 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt 5341 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta 5401 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat 5461 cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca 5521 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac 5581 tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc cgcccagttc 5641 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc 5701 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tagacttttg 5761 cagagacggc ccaaattcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc 5821 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta 5881 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa 5941 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 6001 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 6061 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc 6121 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 6181 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 6241 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 6301 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 6361 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt 6421 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 6481 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 6541 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 6601 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa 6661 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 6721 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 6781 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 6841 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg 6901 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt 6961 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact 7021 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat 7081 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg 7141 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg 7201 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat 7261 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc 7321 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt 7381 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc 7441 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga 7501 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc 7561 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa 7621 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta 7681 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg 7741 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg 7801 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat 7861 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt 7921 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa 7981 aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct 8041 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag 8101 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc 8161 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg 8221 cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga 8281 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc 8341 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc 8401 cagtgccaag ctg //