LOCUS       V046378                 9845 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V046378
VERSION     V046378
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..4866
                     /label="DDR2(NM_006182)"
                     /note="DDR2(NM_006182)"
                     /gene="DDR2"
     CDS             4873..4899
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4903..4929
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4933..4959
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     promoter        5010..5221
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5234..5502
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5535..5933
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /note="confers resistance to blasticidin"
                     /product="blasticidin S deaminase"
                     /transl_table=1
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG*"
                     /label="BSD"
     misc_feature    5940..6528
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6602..6835
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    6907..7028
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7047..7182
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7220..7236)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7244..7260
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7268..7298)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7313..7334
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7622..8210)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8381..9241)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9242..9346)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     9820..9836
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catgatcctg attcccagaa tgctcttggt gctgttcctg
     2341 ctgctgccta tcttgagttc tgcaaaagct caggttaatc cagctatatg ccgctatcct
     2401 ctgggcatgt caggaggcca gattccagat gaggacatca cagcttccag tcagtggtca
     2461 gagtccacag ctgccaaata tggaaggctg gactcagaag aaggggatgg agcctggtgc
     2521 cctgagattc cagtggaacc tgatgacctg aaggagtttc tgcagattga cttgcacacc
     2581 ctccatttta tcactctggt ggggacccag gggcgccatg caggaggtca tggcatcgag
     2641 tttgccccca tgtacaagat caattacagt cgggatggca ctcgctggat ctcttggcgg
     2701 aaccgtcatg ggaaacaggt gctggatgga aatagtaacc cctatgacat tttcctaaag
     2761 gacttggagc cgcccattgt agccagattt gtccggttca ttccagtcac cgaccactcc
     2821 atgaatgtgt gtatgagagt ggagctttac ggctgtgtct ggctagatgg cttggtgtct
     2881 tacaatgctc cagctgggca gcagtttgta ctccctggag gttccatcat ttatctgaat
     2941 gattctgtct atgatggagc tgttggatac agcatgacag aagggctagg ccaattgacc
     3001 gatggtgtgt ctggcctgga cgatttcacc cagacccatg aataccacgt gtggcccggc
     3061 tatgactatg tgggctggcg gaacgagagt gccaccaatg gctacattga gatcatgttt
     3121 gaatttgacc gcatcaggaa tttcactacc atgaaggtcc actgcaacaa catgtttgct
     3181 aaaggtgtga agatctttaa ggaggtacag tgctacttcc gctctgaagc cagtgagtgg
     3241 gaacctaatg ccatttcctt cccccttgtc ctggatgacg tcaaccccag tgctcggttt
     3301 gtcacggtgc ctctccacca ccgaatggcc agtgccatca agtgtcaata ccattttgca
     3361 gatacctgga tgatgttcag tgagatcacc ttccaatcag atgctgcaat gtacaacaac
     3421 tctgaagccc tgcccacctc tcctatggca cccacaacct atgatccaat gcttaaagtt
     3481 gatgacagca acactcggat cctgattggc tgcttggtgg ccatcatctt tatcctcctg
     3541 gccatcattg tcatcatcct ctggaggcag ttctggcaga aaatgctgga gaaggcttct
     3601 cggaggatgc tggatgatga aatgacagtc agcctttccc tgccaagtga ttctagcatg
     3661 ttcaacaata accgctcctc atcacctagt gaacaagggt ccaactcgac ttacgatcgc
     3721 atctttcccc ttcgccctga ctaccaggag ccatccaggc tgatacgaaa actcccagaa
     3781 tttgctccag gggaggagga gtcaggctgc agcggtgttg tgaagccagt ccagcccagt
     3841 ggccctgagg gggtgcccca ctatgcagag gctgacatag tgaacctcca aggagtgaca
     3901 ggaggcaaca catactcagt gcctgccgtc accatggacc tgctctcagg aaaagatgtg
     3961 gctgtggagg agttccccag gaaactccta actttcaaag agaagctggg agaaggacag
     4021 tttggggagg ttcatctctg tgaagtggag ggaatggaaa aattcaaaga caaagatttt
     4081 gccctagatg tcagtgccaa ccagcctgtc ctggtggctg tgaaaatgct ccgagcagat
     4141 gccaacaaga atgccaggaa tgattttctt aaggagataa agatcatgtc tcggctcaag
     4201 gacccaaaca tcatccatct attagctgtg tgtatcactg atgaccctct ctgtatgatc
     4261 actgaataca tggagaatgg agatctcaat cagtttcttt cccgccacga gccccctaat
     4321 tcttcctcca gcgatgtacg cactgtcagt tacaccaatc tgaagtttat ggctacccaa
     4381 attgcctctg gcatgaagta cctttcctct cttaattttg ttcaccgaga tctggccaca
     4441 cgaaactgtt tagtgggtaa gaactacaca atcaagatag ctgactttgg aatgagcagg
     4501 aacctgtaca gtggtgacta ttaccggatc cagggccggg cagtgctccc tatccgctgg
     4561 atgtcttggg agagtatctt gctgggcaag ttcactacag caagtgatgt gtgggccttt
     4621 ggggttactt tgtgggagac tttcaccttt tgtcaagaac agccctattc ccagctgtca
     4681 gatgaacagg ttattgagaa tactggagag ttcttccgag accaagggag gcagacttac
     4741 ctccctcaac cagccatttg tcctgactct gtgtataagc tgatgctcag ctgctggaga
     4801 agagatacga agaaccgtcc ctcattccaa gaaatccacc ttctgctcct tcaacaaggc
     4861 gacgagctcg agtacccata cgacgtacct gattatgcaa cttatcctta tgatgtacct
     4921 gattatgcta gctatcctta tgatgtccca gactacgcat agggatccgc ggccgcgaag
     4981 gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga
     5041 gaagttgggg ggaggggtcg gcaattgaac gggtgcctag agaaggtggc gcggggtaaa
     5101 ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta
     5161 tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca
     5221 gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta cctgaggccg
     5281 ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct cctgaactgc
     5341 gtccgccgtc taggtaagtt taaagctcag gtcgagaccg ggcctttgtc cggcgctccc
     5401 ttggagccta cctagactca gccggctctc cacgctttgc ctgaccctgc ttgctcaact
     5461 ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt gaccggcgcc
     5521 tacgctaggc caccatggcc aagcctttgt ctcaagaaga atccaccctc attgaaagag
     5581 caacggctac aatcaacagc atccccatct ctgaagacta cagcgtcgcc agcgcagctc
     5641 tctctagcga cggccgcatc ttcactggtg tcaatgtata tcattttact gggggacctt
     5701 gtgcagaact cgtggtgctg ggcactgctg ctgctgcggc agctggcaac ctgacttgta
     5761 tcgtcgcgat cggaaatgag aacaggggca tcttgagccc ctgcggacgg tgccgacagg
     5821 tgcttctcga tctgcatcct gggatcaaag ccatagtgaa ggacagtgat ggacagccga
     5881 cggcagttgg gattcgtgaa ttgctgccct ctggttatgt gtgggagggc taagtcgaca
     5941 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     6001 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     6061 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     6121 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     6181 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     6241 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     6301 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg
     6361 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     6421 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     6481 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct ggtaccttta
     6541 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     6601 ctggaagggc taattcactc ccaacgaaaa taagatctgc tttttgcttg tactgggtct
     6661 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     6721 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     6781 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt
     6841 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
     6901 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     6961 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     7021 gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccagt tccgcccatt
     7081 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     7141 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagacttt tgcagagacg
     7201 gcccaaattc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     7261 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     7321 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     7381 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     7441 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     7501 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     7561 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     7621 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     7681 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     7741 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     7801 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     7861 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     7921 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     7981 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     8041 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     8101 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     8161 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     8221 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     8281 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     8341 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     8401 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     8461 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     8521 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     8581 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     8641 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     8701 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     8761 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     8821 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     8881 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     8941 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     9001 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     9061 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     9121 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     9181 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     9241 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     9301 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     9361 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
     9421 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
     9481 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
     9541 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag
     9601 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga
     9661 gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat
     9721 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat
     9781 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca
     9841 agctg
//
