LOCUS       V039212                10651 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V039212
VERSION     V039212
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..5766
                     /label="Ciita(NM_001302618)"
                     /note="Ciita(NM_001302618)"
                     /gene="Ciita"
     promoter        5816..6027
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6040..6308
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             6341..6739
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /note="confers resistance to blasticidin"
                     /product="blasticidin S deaminase"
                     /transl_table=1
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG*"
                     /label="BSD"
     misc_feature    6746..7334
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7408..7641
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7713..7834
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7853..7988
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8026..8042)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8050..8066
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8074..8104)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8119..8140
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8428..9016)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9187..10047)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10048..10152)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10626..10642
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catgaaccac ttccaggcca tcctggccca agtacagaca
     2341 ctgctctcca gccagaagcc caggcaggtg cgggccctcc tggatggcct gctggaagaa
     2401 gagctgctct cacgggaata ccactgtgcc ttgctgcatg agcctgatgg tgatgccctg
     2461 gcccggaaga tttccctgac cctgctggag aaaggggact tagacttgac tttcttgagc
     2521 tgggtctgca acagtctgca ggctcccacg gtagagaggg gcaccagcta cagggaccat
     2581 ggagaccata gtctgtgtgc caccatggat ctgggatctc cagagggcag ctacctggaa
     2641 ctccttaaca gtgatgccga ccccctacat ctctaccacc tctatgacca gatggacctg
     2701 gctggggagg aggagatcga actcagctca gagccagaca cagataccat caactgcgac
     2761 cagttcagca agctgttgca ggacatggaa ctggatgaag agacccggga ggcctatgcc
     2821 aacattgcgg aactggatca gtacgtgttc caggataccc agctcgaggg cctgagcaag
     2881 gacctcttca tagagcacat tggagcagag gaaggctttg gtgagaacat agagatccct
     2941 gtagaagcag gacagaagcc tcagaagaga cgcttcccgg aagagcatgc tatggactca
     3001 aagcacagga agctagtgcc cacctctagg acctcactga actatttgga tctccccact
     3061 gggcacatcc agatcttcac cactctgccc cagggactct ggcaaatctc aggggctggc
     3121 acaggtctct ccagtgtcct aatctaccac ggtgagatgc cccaggtcaa ccaagtgctc
     3181 ccttcaagca gcctcagtat ccccagtctc cccgagtccc cagaccggcc tggctccacc
     3241 agccccttca caccatctgc agctgacctg cccagcatgc ccgaacctgc gctgacctcc
     3301 cgtgtaaatg agacagagga cacatctccc tccccatgcc aagagggtcc cgagtcttcc
     3361 atcaagcttc caaaatggcc agaggctgtg gagcgattcc agcactccct acaggacaaa
     3421 tacaaggcat tgccccagag cccaaggggt cctctggtgg ccgtggagct ggtacgggcc
     3481 aggctggaaa gaggcagcaa caagagccag gaaagggagc tggccactcc cgactggaca
     3541 gagcgccagc tagcccacgg tggtctggca gaggtacttc aggttgtcag tgactgcagg
     3601 cgaccaggag agacacaggt ggtcgctgtg ctgggcaagg ctggccaggg aaagagccac
     3661 tgggccagga cagtgagtca cacctgggca tgtggccagt tgctacaata tgactttgtc
     3721 ttctatgtcc cctgtcattg cttggatcgt cccggggaca cctaccacct gcgggatctg
     3781 ctctgtcccc cgagcctgca gccactggcc atggatgacg aggtccttga ttatatcgtg
     3841 aggcagccag accgtgttct gctcatccta gatgctttcg aggagctaga ggcccaagat
     3901 ggcctcctgc acggaccctg tggatctctg tccccagagc cctgctccct ccgaggactg
     3961 ctggctggga tcttccagcg gaagctactg cgaggctgca cactgctcct cacagcccgg
     4021 ccccggggcc gcctggctca gagcctgagc aaggcagatg ccatctttga ggtgcccagc
     4081 ttctctacca agcaggccaa gacttacatg aggcactact ttgagaactc agggacagcg
     4141 gggaaccaag acaaggccct gggcctcctg gagggccagc ctcttctctg cagctatagt
     4201 cacagccctg ttgtgtgcag ggctgtgtgc cagctctcca aggccctgct agaacagggc
     4261 acagaggccc agctaccttg tacacttaca ggactctatg tcagcctgct aggtcctgca
     4321 gctcagaaca gtcctcccgg agccttagtc gagctggcca agctggcctg ggagctggga
     4381 cgaagacacc aaagcacctt gcaagaaacc cggttttcat ccgtggaggt gaaaacctgg
     4441 gcagtgaccc aaggcttgat gcagcagacc ctggagacca cggaggctca actggccttc
     4501 tccagttttc tgctacagtg tttcctgggt gctgtgtggc tggcacagtg caatgaaatc
     4561 aaagacaagg agctgccaca gtacctggcc ttgactccga ggaagaagag accctatgac
     4621 aactggctgg agggtgtacc acgctttctg gctggattag ttttccagcc tcgagcccac
     4681 tgcctgggag ctctggtgga gcctgcagtg gctgcagtgg cggataggaa acagaaggtt
     4741 cttaccaggt acctgaagcg cctgaagctg gggacactcc gggcagggag gctgctggag
     4801 ctgctccact gtgcccacga gacacagcaa cctgggatat gggagcatgt tgcacaccag
     4861 ctccctggcc acctctcctt cctgggcacc cggctcacac ccccagatgt gtatgtgctg
     4921 ggcagggcct tggagacagc cagccaggac ttctccttgg accttcgtca gactggcgtt
     4981 gagccttctg gactgggaaa cctcgtggga ctcagctgtg tcaccagttt cagggcctcc
     5041 ttgagtgata caatggcatt atgggagtcc cttcagcagc agggagaagc ccagctactc
     5101 caggcggcag aggagaagtt caccattgag ccatttaaag ccaaatcccc aaaggatgtg
     5161 gaagacctgg atcgtctcgt gcagacccag aggctgagaa acccctcaga agatgcagcc
     5221 aaggatcttc ctgccatccg ggaccttaag aagctagagt ttgcgttggg ccccatcttg
     5281 ggcccccagg ctttccccac actggcaaag atccttccag ccttctcttc tctgcaacac
     5341 ctggacctgg actcacttag tgagaacaag atcggagaca agggtgtgtc gaagctctca
     5401 gccaccttcc ctcagctgaa ggccctggag acgctcaact tgtcccaaaa caacatcact
     5461 gatgtgggtg cctgcaagct tgcagaagct ctgccagccc tagccaagtc cctcctaagg
     5521 ctgagcttgt acaataactg catctgtgac aaaggagcca agagcctggc acaagtactt
     5581 ccggacatgg tgtccctgcg tgtgatggat gtccagttca acaagttcac ggctgccggt
     5641 gcccagcaac tggcctccag ccttcagaag tgccctcagg tggaaacact ggcaatgtgg
     5701 acacccacta tcccctttgg ggttcaggaa cacctgcagc agctggatgc caggatcagt
     5761 ctgagatagg atccgcggcc gcgaaggatc tgcgatcgct ccggtgcccg tcagtgggca
     5821 gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaacgggt
     5881 gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt
     5941 tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt
     6001 cgcaacgggt ttgccgccag aacacagctg aagcttcgag gggctcgcat ctctccttca
     6061 cgcgcccgcc gccctacctg aggccgccat ccacgccggt tgagtcgcgt tctgccgcct
     6121 cccgcctgtg gtgcctcctg aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg
     6181 agaccgggcc tttgtccggc gctcccttgg agcctaccta gactcagccg gctctccacg
     6241 ctttgcctga ccctgcttgc tcaactctac gtctttgttt cgttttctgt tctgcgccgt
     6301 tacagatcca agctgtgacc ggcgcctacg ctaggccacc atggccaagc ctttgtctca
     6361 agaagaatcc accctcattg aaagagcaac ggctacaatc aacagcatcc ccatctctga
     6421 agactacagc gtcgccagcg cagctctctc tagcgacggc cgcatcttca ctggtgtcaa
     6481 tgtatatcat tttactgggg gaccttgtgc agaactcgtg gtgctgggca ctgctgctgc
     6541 tgcggcagct ggcaacctga cttgtatcgt cgcgatcgga aatgagaaca ggggcatctt
     6601 gagcccctgc ggacggtgcc gacaggtgct tctcgatctg catcctggga tcaaagccat
     6661 agtgaaggac agtgatggac agccgacggc agttgggatt cgtgaattgc tgccctctgg
     6721 ttatgtgtgg gagggctaag tcgacaatca acctctggat tacaaaattt gtgaaagatt
     6781 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     6841 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     6901 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     6961 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     7021 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     7081 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     7141 atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     7201 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     7261 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     7321 ggccgcctcc ccgcctggta cctttaagac caatgactta caaggcagct gtagatctta
     7381 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaaaataag
     7441 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     7501 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     7561 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     7621 cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag tatttataac
     7681 ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt
     7741 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     7801 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc
     7861 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     7921 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     7981 tggaggccta gacttttgca gagacggccc aaattcgtaa tcatggtcat agctgtttcc
     8041 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     8101 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     8161 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     8221 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     8281 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     8341 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     8401 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     8461 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     8521 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     8581 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     8641 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     8701 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     8761 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     8821 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
     8881 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     8941 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     9001 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     9061 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     9121 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     9181 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     9241 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     9301 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     9361 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     9421 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     9481 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     9541 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     9601 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     9661 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     9721 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     9781 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     9841 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     9901 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     9961 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
    10021 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
    10081 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
    10141 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
    10201 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg
    10261 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
    10321 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
    10381 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg
    10441 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag
    10501 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
    10561 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
    10621 acgttgtaaa acgacggcca gtgccaagct g
//
