LOCUS       V041711                 9374 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V041711
VERSION     V041711
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2290..3738
                     /label="Ccdc47(NM_026009)"
                     /note="Ccdc47(NM_026009)"
                     /gene="Ccdc47"
     CDS             3781..4491
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK*"
                     /label="mCherry"
     promoter        4539..4750
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4763..5031
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5064..5462
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /note="confers resistance to blasticidin"
                     /product="blasticidin S deaminase"
                     /transl_table=1
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG*"
                     /label="BSD"
     misc_feature    5469..6057
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6131..6364
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    6436..6557
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      6576..6711
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(6749..6765)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6773..6789
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6797..6827)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6842..6863
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7151..7739)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7910..8770)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8771..8875)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     9349..9365
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagccacca tgaaagcctt ctatgctttc tgtgtggtcc tcttggtgtt tgggagtgtc
     2341 tcggaagcca agtttgatga tttcgaagat gaggaagaca tagtagagta tgatgataat
     2401 gattttgctg agtttgagga cgtcatggaa gattctgtta cggagtcgcc tcagcgagtg
     2461 atcagcactg aagatgacga ggacgaggcc accgtggagt tggaagggca ggatgaaagc
     2521 caagaaggtg atttcgaaga tgcagatacc caggagggag atacagaaag tgagccatat
     2581 gatgatgagg aatttgaggg ttatgaagac aaaccagata cctcttctaa caaaaataaa
     2641 gatccaataa caattgttga tgttcctgca cacctccaga acagttggga gagttattac
     2701 ctagaaattt tgatggtgac tggtctgctt gcctatatca tgaactacat cattgggaag
     2761 aataaaaaca gccgacttgc tcaggcctgg tttaactctc atagagagct tttggagagc
     2821 aattttacat tagtggggga tgatgggact aacaaagaag ccacaagcac agggaagttg
     2881 aatcaggaga atgagcacat ctataacctg tggtgttctg gccgagtgtg ctgtgaaggc
     2941 atgcttatcc agctgaggtt ccttaagaga caagacttac ttaatgtcct ggcccggatg
     3001 atgaggccag tgagtgatca agtgcaaata aaagtaacaa tgaatgacga ggacatggac
     3061 acatacgtgt ttgctgtcgg cactcgcaaa gctttgctgc gactacagaa agagatgcag
     3121 gatctgagtg agttttgcag tgataaacca aagtctggag caaagtatgg actgccagac
     3181 tctttggcca ttctgtcaga aatgggagaa gtcacagagg gaatgatgga tacaaagatg
     3241 gttcattttc ttacacacta tgctgataag attgaatctg ttcatttttc agaccagttc
     3301 tctggtccaa agattatgca agaggaaggc cagcctttaa agctgcctga caccaagagg
     3361 acgctactgt ttacatttaa tgtgcctggc tcaggtaaca catacccaaa ggatatggag
     3421 tctttgctac ccctgatgaa catggtgatt tattctatcg ataaagccaa aaagttccga
     3481 ctcaacagag aagggaaaca aaaagcagat aagaaccggg ctcgtgtgga agagaacttt
     3541 ctgaagctga cacatgtgca gagacaggag gctgcacagt ctcggcgtga ggagaaaaaa
     3601 agagctgaga aggagcggat catgaacgag gaggaccctg agaaacagcg caggctggaa
     3661 gaagctgctt tgaggagaga acaaaagaag ttggagaaga agcaaatgaa aatgaaacaa
     3721 atcaaagtga aagccatggg tggaggcgga tcaggaggcg gtggatctga attcgccacc
     3781 atggtgagca agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag
     3841 gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc
     3901 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc
     3961 ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac
     4021 cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc
     4081 gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac
     4141 ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta
     4201 atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc
     4261 gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct
     4321 gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc
     4381 aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa
     4441 cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaagta gggatccgcg
     4501 gccgcgaagg atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca catcgcccac
     4561 agtccccgag aagttggggg gaggggtcgg caattgaacg ggtgcctaga gaaggtggcg
     4621 cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg
     4681 agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc
     4741 cagaacacag ctgaagcttc gaggggctcg catctctcct tcacgcgccc gccgccctac
     4801 ctgaggccgc catccacgcc ggttgagtcg cgttctgccg cctcccgcct gtggtgcctc
     4861 ctgaactgcg tccgccgtct aggtaagttt aaagctcagg tcgagaccgg gcctttgtcc
     4921 ggcgctccct tggagcctac ctagactcag ccggctctcc acgctttgcc tgaccctgct
     4981 tgctcaactc tacgtctttg tttcgttttc tgttctgcgc cgttacagat ccaagctgtg
     5041 accggcgcct acgctaggcc accatggcca agcctttgtc tcaagaagaa tccaccctca
     5101 ttgaaagagc aacggctaca atcaacagca tccccatctc tgaagactac agcgtcgcca
     5161 gcgcagctct ctctagcgac ggccgcatct tcactggtgt caatgtatat cattttactg
     5221 ggggaccttg tgcagaactc gtggtgctgg gcactgctgc tgctgcggca gctggcaacc
     5281 tgacttgtat cgtcgcgatc ggaaatgaga acaggggcat cttgagcccc tgcggacggt
     5341 gccgacaggt gcttctcgat ctgcatcctg ggatcaaagc catagtgaag gacagtgatg
     5401 gacagccgac ggcagttggg attcgtgaat tgctgccctc tggttatgtg tgggagggct
     5461 aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     5521 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     5581 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     5641 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     5701 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     5761 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     5821 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt
     5881 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     5941 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     6001 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg
     6061 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     6121 aaggggggac tggaagggct aattcactcc caacgaaaat aagatctgct ttttgcttgt
     6181 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     6241 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     6301 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     6361 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata
     6421 tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     6481 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     6541 ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccagtt
     6601 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     6661 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagactttt
     6721 gcagagacgg cccaaattcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     6781 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     6841 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     6901 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     6961 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     7021 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     7081 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     7141 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     7201 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     7261 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     7321 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     7381 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     7441 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     7501 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     7561 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
     7621 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     7681 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     7741 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     7801 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     7861 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     7921 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     7981 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     8041 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     8101 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     8161 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     8221 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     8281 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     8341 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     8401 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     8461 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     8521 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     8581 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     8641 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     8701 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     8761 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     8821 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     8881 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
     8941 aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc
     9001 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
     9061 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg
     9121 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat
     9181 gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg
     9241 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg
     9301 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg
     9361 ccagtgccaa gctg
//
