LOCUS       V045400                10453 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V045400
VERSION     V045400
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2284..2991
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             3028..5544
                     /label="ATG9A(NM_001077198)"
                     /note="ATG9A(NM_001077198)"
                     /gene="ATG9A"
     CDS             5551..5577
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     promoter        5618..5829
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5842..6110
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             6143..6541
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /note="confers resistance to blasticidin"
                     /product="blasticidin S deaminase"
                     /transl_table=1
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG*"
                     /label="BSD"
     misc_feature    6548..7136
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7210..7443
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7515..7636
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7655..7790
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7828..7844)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7852..7868
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7876..7906)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7921..7942
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8230..8818)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8989..9849)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9850..9954)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10428..10444
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagatgcc
     2281 accatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc
     2341 aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag
     2401 ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg
     2461 cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag
     2521 caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag
     2581 cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag
     2641 gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc
     2701 gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac
     2761 ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac
     2821 gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac
     2881 gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac
     2941 gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa gggaggtgga
     3001 ggtagtggag gaggcggatc gtctagaatg gcgcagtttg acactgaata ccagcgccta
     3061 gaggcctcct atagtgattc acccccaggg gaggaggacc tgttggtgca cgtcgccgag
     3121 gggagcaagt caccttggca ccatattgaa aaccttgacc tcttcttctc tcgagtttat
     3181 aatctgcacc agaagaatgg cttcacatgt atgctcatcg gggagatctt tgagctcatg
     3241 cagttcctct ttgtggttgc cttcactacc ttcctggtca gctgcgtgga ctatgacatc
     3301 ctatttgcca acaagatggt gaaccacagt cttcacccta ctgaacccgt caaggtcact
     3361 ctgccagacg cctttttgcc tgctcaagtc tgtagtgcca ggattcagga aaatggctcc
     3421 cttatcacca tcctggtcat tgctggtgtc ttctggatcc accggcttat caagttcatc
     3481 tataacattt gctgctactg ggagatccac tccttctacc tgcacgctct gcgcatccct
     3541 atgtctgccc ttccgtattg cacgtggcaa gaagtgcagg cccggatcgt gcagacgcag
     3601 aaggagcacc agatctgcat ccacaaacgt gagctgacag aactggacat ctaccaccgc
     3661 atcctccgtt tccagaacta catggtggca ctggttaaca aatccctcct gcctctgcgc
     3721 ttccgcctgc ctggcctcgg ggaagctgtc ttcttcaccc gtggtctcaa gtacaacttt
     3781 gagctgatcc tcttctgggg acctggctct ctgtttctca atgaatggag cctcaaggcc
     3841 gagtacaaac gtggggggca acggctagag ctggcccagc gcctcagcaa ccgcatcctg
     3901 tggattggca tcgctaactt cctgctgtgc cccctcatcc tcatatggca aatcctctat
     3961 gccttcttca gctatgctga ggtgctgaag cgggagccgg gggccctggg agcacgctgc
     4021 tggtcactct atggccgctg ctacctccgc cacttcaacg agctggagca cgagctgcag
     4081 tcccgcctca accgtggcta caagcccgcc tccaagtaca tgaattgctt cttgtcacct
     4141 cttttgacac tgctggccaa gaatggagcc ttcttcgctg gctccatcct ggctgtgctt
     4201 attgccctca ccatttatga cgaagatgtg ttggctgtgg aacatgtgct gaccaccgtc
     4261 acactcctgg gggtcaccgt gaccgtgtgc aggtccttta tcccggacca gcacatggtg
     4321 ttctgccctg agcagctgct ccgcgtgatc ctcgctcaca tccactacat gcctgaccac
     4381 tggcagggta atgcccaccg ctcgcagacc cgggacgagt ttgcccagct cttccagtac
     4441 aaggcagtgt tcattttgga agagttgctg agccccattg tcacacccct catcctcatc
     4501 ttctgcctgc gcccacgggc cctggagatt atagacttct tccgaaactt caccgtggag
     4561 gtcgttggtg tgggagatac ctgctccttt gctcagatgg atgttcgcca gcatggtcat
     4621 ccccagtggc tatctgctgg gcagacagag gcctcagtgt accagcaagc tgaggatgga
     4681 aagacagagt tgtcactcat gcactttgcc atcaccaacc ctggctggca gccaccacgt
     4741 gagagcacag ccttcctagg cttcctcaag gagcaggttc agcgggatgg agcagctgct
     4801 agcctcgccc aagggggtct gctccctgaa aatgccctct ttacgtctat ccagtcctta
     4861 caatctgagt ctgagcccct gagccttatc gcaaatgtgg tagctggctc atcctgccgg
     4921 ggccctccac tgcccagaga cctgcagggc tccaggcaca gggctgaagt cgcctctgcc
     4981 ctgcgctcct tctccccgct gcaacccggg caggcgccca caggccgggc tcacagcacc
     5041 atgacaggct ctggggtgga tgccaggaca gccagctccg ggagcagcgt gtgggaagga
     5101 cagctgcaga gcctggtgct gtcagaatat gcatccacag agatgagcct gcatgccctc
     5161 tatatgcacc agctccacaa gcagcaggcc caggctgaac ctgagcggca tgtatggcac
     5221 cgccgggaga gtgatgagag tggagaaagc gcccctgatg aagggggaga gggcgcccgg
     5281 gccccccagt ctatccctcg ctctgctagc tatccctgtg cagcaccccg gcctggagct
     5341 cctgagacca ctgccctgca tgggggcttc cagaggcgct acggtggcat cacagatcct
     5401 ggcacagtgc ccagggttcc ctctcatttc tctcggctgc ctcttggagg gtgggcagaa
     5461 gatgggcagt cggcatcaag gcaccctgag cccgtgcccg aagagggctc ggaggatgag
     5521 ctaccccctc aggtgcacaa ggtactcgag tacccatacg acgtaccaga ttacgcttga
     5581 attcgaagga tctgcgatcg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca
     5641 gtccccgaga agttgggggg aggggtcggc aattgaacgg gtgcctagag aaggtggcgc
     5701 ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga
     5761 gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc
     5821 agaacacagc tgaagcttcg aggggctcgc atctctcctt cacgcgcccg ccgccctacc
     5881 tgaggccgcc atccacgccg gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc
     5941 tgaactgcgt ccgccgtcta ggtaagttta aagctcaggt cgagaccggg cctttgtccg
     6001 gcgctccctt ggagcctacc tagactcagc cggctctcca cgctttgcct gaccctgctt
     6061 gctcaactct acgtctttgt ttcgttttct gttctgcgcc gttacagatc caagctgtga
     6121 ccggcgccta cgctaggcca ccatggccaa gcctttgtct caagaagaat ccaccctcat
     6181 tgaaagagca acggctacaa tcaacagcat ccccatctct gaagactaca gcgtcgccag
     6241 cgcagctctc tctagcgacg gccgcatctt cactggtgtc aatgtatatc attttactgg
     6301 gggaccttgt gcagaactcg tggtgctggg cactgctgct gctgcggcag ctggcaacct
     6361 gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc ttgagcccct gcggacggtg
     6421 ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc atagtgaagg acagtgatgg
     6481 acagccgacg gcagttggga ttcgtgaatt gctgccctct ggttatgtgt gggagggcta
     6541 agtcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     6601 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     6661 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     6721 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     6781 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     6841 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     6901 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
     6961 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     7021 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     7081 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg
     7141 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     7201 aggggggact ggaagggcta attcactccc aacgaaaata agatctgctt tttgcttgta
     7261 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     7321 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     7381 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     7441 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
     7501 cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     7561 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     7621 tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc cgcccagttc
     7681 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     7741 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tagacttttg
     7801 cagagacggc ccaaattcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     7861 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     7921 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     7981 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     8041 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     8101 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     8161 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     8221 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     8281 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     8341 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     8401 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     8461 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     8521 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     8581 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     8641 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
     8701 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     8761 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     8821 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     8881 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     8941 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     9001 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     9061 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     9121 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     9181 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     9241 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     9301 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     9361 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     9421 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     9481 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     9541 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     9601 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     9661 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     9721 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     9781 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     9841 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     9901 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     9961 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
    10021 aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct
    10081 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
    10141 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc
    10201 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg
    10261 cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga
    10321 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
    10381 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
    10441 cagtgccaag ctg
//
