LOCUS       V042122                 8984 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V042122
VERSION     V042122
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..4098
                     /label="AFM(NM_001133)"
                     /note="AFM(NM_001133)"
                     /gene="AFM"
     promoter        4149..4360
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4373..4641
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             4674..5072
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /note="confers resistance to blasticidin"
                     /product="blasticidin S deaminase"
                     /transl_table=1
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG*"
                     /label="BSD"
     misc_feature    5079..5667
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             5741..5974
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    6046..6167
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      6186..6321
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(6359..6375)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6383..6399
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6407..6437)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6452..6473
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6761..7349)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7520..8380)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8381..8485)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     8959..8975
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catgaaacta ctaaaactta caggttttat ttttttcttg
     2341 ttttttttga ctgaatccct aaccctgccc acacaacctc gggatataga gaacttcaat
     2401 agtactcaaa aatttataga agataatatt gaatacatca ccatcattgc atttgctcag
     2461 tatgttcagg aagcaacctt tgaagaaatg gaaaagctgg tgaaagacat ggtagaatac
     2521 aaagacagat gtatggctga caagacgctc ccagagtgtt caaaattacc taataatgtt
     2581 ttacaggaaa aaatatgtgc tatggagggg ctgccacaaa agcataattt ctcacactgc
     2641 tgcagtaagg ttgatgctca aagaagactc tgtttcttct ataacaagaa atctgatgtg
     2701 ggatttctgc ctcctttccc taccctggat cccgaagaga aatgccaggc ttatgaaagt
     2761 aacagagaat cccttttaaa tcacttttta tatgaagttg ccagaaggaa cccatttgtc
     2821 ttcgccccta cacttctaac tgttgctgtt cattttgagg aggtggccaa atcatgttgt
     2881 gaagaacaaa acaaagtcaa ctgccttcaa acaagggcaa tacctgtcac acaatattta
     2941 aaagcatttt cttcttatca aaaacatgtc tgtggggcac ttttgaaatt tggaaccaaa
     3001 gttgtacact ttatatatat tgcgatactc agtcaaaaat tccccaagat tgaatttaag
     3061 gagcttattt ctcttgtaga agatgtttct tccaactatg atggatgctg tgaaggggat
     3121 gttgtgcagt gcatccgtga cacgagcaag gttatgaacc atatttgttc aaaacaagat
     3181 tctatctcca gcaaaatcaa agagtgctgt gaaaagaaaa taccagagcg cggccagtgc
     3241 ataattaact caaacaaaga tgatagacca aaggatttat ctctaagaga aggaaaattt
     3301 actgacagtg aaaatgtgtg tcaagaacga gatgctgacc cagacacctt ctttgcgaag
     3361 tttacttttg aatactcaag gagacatcca gacctgtcta taccagagct tttaagaatt
     3421 gttcaaatat acaaagatct cctgagaaat tgctgcaaca cagaaaaccc tccaggttgt
     3481 taccgttacg cggaagacaa attcaatgag acaactgaga aaagcctcaa gatggtacaa
     3541 caagaatgta aacatttcca gaatttgggg aaggatggtt tgaaatacca ttacctcatc
     3601 aggctcacga agatagctcc ccaactctcc actgaagaac tggtgtctct tggcgagaaa
     3661 atggtgacag ctttcactac ttgctgtacg ctaagtgaag agtttgcctg tgttgataat
     3721 ttggcagatt tagtttttgg agagttatgt ggagtaaatg aaaatcgaac tatcaaccct
     3781 gctgtggacc actgctgtaa aacaaacttt gccttcagaa ggccctgctt tgagagtttg
     3841 aaagctgata aaacatatgt gcctccacct ttctctcaag atttatttac ctttcacgca
     3901 gacatgtgtc aatctcagaa tgaggagctt cagaggaaga cagacaggtt tcttgtcaac
     3961 ttagtgaagc tgaagcatga actcacagat gaagagctgc agtctttgtt tacaaatttc
     4021 gcaaatgtag tggataagtg ctgcaaagca gagagtcctg aagtctgctt taatgaagag
     4081 agtccaaaaa ttggcaactg aggatccgcg gccgcgaagg atctgcgatc gctccggtgc
     4141 ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg
     4201 caattgaacg ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta
     4261 ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt
     4321 gaacgttctt tttcgcaacg ggtttgccgc cagaacacag ctgaagcttc gaggggctcg
     4381 catctctcct tcacgcgccc gccgccctac ctgaggccgc catccacgcc ggttgagtcg
     4441 cgttctgccg cctcccgcct gtggtgcctc ctgaactgcg tccgccgtct aggtaagttt
     4501 aaagctcagg tcgagaccgg gcctttgtcc ggcgctccct tggagcctac ctagactcag
     4561 ccggctctcc acgctttgcc tgaccctgct tgctcaactc tacgtctttg tttcgttttc
     4621 tgttctgcgc cgttacagat ccaagctgtg accggcgcct acgctaggcc accatggcca
     4681 agcctttgtc tcaagaagaa tccaccctca ttgaaagagc aacggctaca atcaacagca
     4741 tccccatctc tgaagactac agcgtcgcca gcgcagctct ctctagcgac ggccgcatct
     4801 tcactggtgt caatgtatat cattttactg ggggaccttg tgcagaactc gtggtgctgg
     4861 gcactgctgc tgctgcggca gctggcaacc tgacttgtat cgtcgcgatc ggaaatgaga
     4921 acaggggcat cttgagcccc tgcggacggt gccgacaggt gcttctcgat ctgcatcctg
     4981 ggatcaaagc catagtgaag gacagtgatg gacagccgac ggcagttggg attcgtgaat
     5041 tgctgccctc tggttatgtg tgggagggct aagtcgacaa tcaacctctg gattacaaaa
     5101 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     5161 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     5221 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     5281 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     5341 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     5401 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     5461 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc
     5521 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     5581 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     5641 ggatctccct ttgggccgcc tccccgcctg gtacctttaa gaccaatgac ttacaaggca
     5701 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
     5761 caacgaaaat aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga
     5821 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     5881 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     5941 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt
     6001 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca
     6061 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     6121 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc
     6181 tagctatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     6241 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     6301 gaggaggctt ttttggaggc ctagactttt gcagagacgg cccaaattcg taatcatggt
     6361 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     6421 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     6481 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     6541 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     6601 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     6661 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     6721 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     6781 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     6841 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     6901 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     6961 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     7021 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     7081 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     7141 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     7201 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     7261 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     7321 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     7381 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     7441 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     7501 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     7561 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     7621 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     7681 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     7741 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     7801 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     7861 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     7921 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     7981 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     8041 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     8101 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     8161 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     8221 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     8281 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     8341 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     8401 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     8461 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag
     8521 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
     8581 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     8641 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     8701 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     8761 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
     8821 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     8881 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     8941 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctg
//
