LOCUS       V039169                11047 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V039169
VERSION     V039169
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..5073
                     /label="Bclaf1(NM_001025392)"
                     /note="Bclaf1(NM_001025392)"
                     /gene="Bclaf1"
     CDS             5080..5145
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5189..5400
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5413..5681
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5710..5719
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5740..6402
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6472..6525
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6526..7125
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7126..7714
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7788..8021
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8093..8214
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8254..8389
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8422..8438)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8446..8462
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8470..8500)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8515..8536
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8824..9412)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9583..10443)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10444..10548)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11022..11038
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg gccgctccaa ttctagatca
     2341 cattcttcaa gatccaagtc tagatcacag tctagttcta gatcaagatc aagatcacat
     2401 tctagaaaga agagatacag ttctaggtct cgttccagaa catattcgag gtctcgtagt
     2461 agagatcgta tttattctag agattatcgt cgagattaca ggaataatag aggaatgaga
     2521 cgaccttatg ggtacagagg aaggggtaga gggtattatc aaggaggagg agggagatac
     2581 catcgaggtg gctatagacc tgtctggaat agaaggcact ctaggagtcc tagacgaggt
     2641 cggtcacggt ccaggagtcc aaaaagaaga tccgtgtctt ctcaaagatc ccgaagcaga
     2701 tctcgccggt catatagatc ctctaggtct ccaagatcat catcatctcg ctcttcatcc
     2761 ccatatagca aatctcctgt ctctaaaaga cgagggtctc aggaaaaaca aaccaaaaaa
     2821 gctgaggggg aaccccaaga agagagtcct ttgaaaagca aatcacagga ggaaccaaag
     2881 gatacttttg aacatgatcc atctgaatct attgatgagt ttaataaatc tgctacttct
     2941 ggtgatattt ggcctggcct ttcagcttat gataatagtc caaggtcacc tcatagtcct
     3001 tcacctattg ctacaccacc cagtcagagt tcatcatgct cagatgcccc catgcttagt
     3061 acagtgcact ctgccaaaaa taccccttct cagcattcac attccattca gcacagtcct
     3121 gagaggtctg gatctggctc tgttggaaat gggtcgagtc gatatagtcc ttctcagaat
     3181 agtccgattc atcacatccc atcacgaaga agccctgcca agacaatcac accacagaat
     3241 gctccaagag aggaatctag ggggcggtcc tccttttatc ctgaaggaga tcaggaaaca
     3301 gcaaagacag gaaagttttt aaaaaggttc acagatgaag agtctagagt attcctgctt
     3361 gataggggta atatcaggga taaagaggct ccgaaggaga aagggtcaga gaaaggcagg
     3421 gcagatggag attgggatga ccaggaagtt ctagattact ttagtgataa agagtctgca
     3481 aaacaaaaat tccatgactc tgaaggggat gacacagagg agacagagga ttatagacag
     3541 tttaggaaat cagtcctagc agatcagggc aagagctttg ctacttcatc tcaccggaat
     3601 actgaggagg aaggacccaa gtacaagtcc aaagtttcat taaaaggcaa tagagaaagt
     3661 gatggattta gagaagaaaa aaattataaa ctgaaagaga ctgcatacat agtcgaaagg
     3721 cctagcactg caaaggacaa gcacaaggaa gaagacaaag gctctgatag aataacagtg
     3781 aagaaagaag tgcagtcacc ggagcaggta aagtctgaaa agctcaaaga gctctttgat
     3841 tacagtcccc ctctacacaa gagtctggat gcacgagaaa agtccatctt cagagaggag
     3901 agcccactga ggatcaaaat gatagccagt gattctcacc gtcctgaagt caaactcaaa
     3961 atggcacctg ttcctcttga cgattccaac agacctgcct ccttgactaa agacaggcta
     4021 cttgctagca cgcttgtcca ttctgtcaag aaagagcaag aattccgatc catctttgac
     4081 cacattaagc tgcctcaggc caacaaaagc acttcagaat catttataca acacattgta
     4141 tccttggttc atcatgtgaa agaacaatac ttcaaatcac ctgcagtgac cctaaacgag
     4201 cggttcactt cataccagaa agctactgaa gaacatagta cccggcaaaa gagccccgag
     4261 atacacagga ggattgacat ctctcccagc gctctgagga agcacacccg gctagcagga
     4321 gaggagagag gttttaaaga agaaattcag aagggagata aaaagttaag gtgtgattcg
     4381 gctgaccttc ggcacgacat tgaccgtcgc cgtaaagaga gaagtaaaga gcggggagat
     4441 tccaagggct ccagggagtc cagtgggtcc agaaagcaag aaaaaacacc aaaggattac
     4501 aaggagtata aaccttacaa agatgacagc aaacataaag gtagagagcg ggaccattcc
     4561 cggtcatcgt cctcgtcagc atcgccgtcc tcgcccagtt ctcgagaaga aaaagagagt
     4621 aagaaagaga gagaagaaga atttaaaact caccacgaga tgaaagacta ctcaggattt
     4681 gcaggagtta gcagaccacg aggaaccttt tttcgaatta gaggcagagg aagagccaga
     4741 ggagtttttg ctgggacaaa tactggtcca aacaactcaa ataccacttt tcaaaagaga
     4801 ccgaaggaag aggaatggga tccagaatat accccaaaga gcaagaagta cttcttgcac
     4861 gatgacagag atgatggtgt ggattactgg gccaaaagag gaagaggtcg tggtactttt
     4921 caacgtggca gagggcgctt taatttcaaa aaatcaggta gcagtccaaa atggactcat
     4981 gacaaatacc aaggagatgg gattgttgaa gatgacgaag agaccatgga aaataatgaa
     5041 gagaagaagg acagacgcaa agaagaaaag gaaggatccg actacaaaga ccatgacggt
     5101 gattataaag atcatgacat cgactacaag gatgacgatg acaagtagcg gccgcgaagg
     5161 atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag
     5221 aagttggggg gaggggtcgg caattgaacg ggtgcctaga gaaggtggcg cggggtaaac
     5281 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat
     5341 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag
     5401 ctgaagcttc gaggggctcg catctctcct tcacgcgccc gccgccctac ctgaggccgc
     5461 catccacgcc ggttgagtcg cgttctgccg cctcccgcct gtggtgcctc ctgaactgcg
     5521 tccgccgtct aggtaagttt aaagctcagg tcgagaccgg gcctttgtcc ggcgctccct
     5581 tggagcctac ctagactcag ccggctctcc acgctttgcc tgaccctgct tgctcaactc
     5641 tacgtctttg tttcgttttc tgttctgcgc cgttacagat ccaagctgtg accggcgcct
     5701 acgctagacg ccaccatgga gagcgacgag agcggcctgc ccgccatgga gatcgagtgc
     5761 cgcatcaccg gcaccctgaa cggcgtggag ttcgagctgg tgggcggcgg agagggcacc
     5821 cccaagcagg gccgcatgac caacaagatg aagagcacca aaggcgccct gaccttcagc
     5881 ccctacctgc tgagccacgt gatgggctac ggcttctacc acttcggcac ctaccccagc
     5941 ggctacgaga accccttcct gcacgccatc aacaacggcg gctacaccaa cacccgcatc
     6001 gagaagtacg aggacggcgg cgtgctgcac gtgagcttca gctaccgcta cgaggccggc
     6061 cgcgtgatcg gcgacttcaa ggtggtgggc accggcttcc ccgaggacag cgtgatcttc
     6121 accgacaaga tcatccgcag caacgccacc gtggagcacc tgcaccccat gggcgataac
     6181 gtgctggtgg gcagcttcgc ccgcaccttc agcctgcgcg acggcggcta ctacagcttc
     6241 gtggtggaca gccacatgca cttcaagagc gccatccacc ccagcatcct gcagaacggg
     6301 ggccccatgt tcgccttccg ccgcgtggag gagctgcaca gcaacaccga gctgggcatc
     6361 gtggagtacc agcacgcctt caagaccccc atcgccttcg ccagatcccg cgctcagtcg
     6421 tccaattctg ccgtggacgg caccgccgga cccggctcca ccggatctcg cgagggcaga
     6481 ggaagtcttc taacatgcgg tgacgtggag gagaatcccg gccctatgac cgagtacaag
     6541 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc
     6601 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg
     6661 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
     6721 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg
     6781 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg
     6841 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg
     6901 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
     6961 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc
     7021 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
     7081 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgaaatca acctctggat
     7141 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     7201 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     7261 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     7321 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     7381 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     7441 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     7501 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc
     7561 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     7621 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     7681 acgagtcgga tctccctttg ggccgcctcc ccgcctggta cctttaagac caatgactta
     7741 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     7801 tcactcccaa cgaagataag atctgctttt tgcttgtact gggtctctct ggttagacca
     7861 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     7921 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     7981 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct
     8041 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt
     8101 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     8161 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     8221 ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     8281 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     8341 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct agacttttgc
     8401 agagaccaaa ttcgtaatca tgtcatagct gtttcctgtg tgaaattgtt atccgctcac
     8461 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     8521 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     8581 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     8641 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     8701 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     8761 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     8821 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     8881 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     8941 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     9001 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     9061 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     9121 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     9181 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     9241 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
     9301 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     9361 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     9421 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     9481 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     9541 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     9601 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     9661 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     9721 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     9781 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     9841 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     9901 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     9961 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
    10021 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
    10081 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
    10141 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
    10201 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
    10261 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
    10321 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
    10381 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
    10441 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
    10501 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
    10561 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
    10621 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca
    10681 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
    10741 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc
    10801 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag
    10861 gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg
    10921 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
    10981 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtgc
    11041 caagctg
//
