LOCUS       V027620                12541 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V027620
VERSION     V027620
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..6567
                     /label="BLM(NM_000057)"
                     /note="BLM(NM_000057)"
                     /gene="BLM"
     CDS             6574..6639
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        6683..6894
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6907..7175
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      7204..7213
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             7234..7896
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             7966..8019
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             8020..8619
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    8620..9208
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             9282..9515
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    9587..9708
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      9748..9883
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(9916..9932)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9940..9956
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9964..9994)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    10009..10030
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(10318..10906)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(11077..11937)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11938..12042)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     12516..12532
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg ctgctgttcc tcaaaataat
     2341 ctacaggagc aactagaacg tcactcagcc agaacactta ataataaatt aagtctttca
     2401 aaaccaaaat tttcaggttt cacttttaaa aagaaaacat cttcagataa caatgtatct
     2461 gtaactaatg tgtcagtagc aaaaacacct gtattaagaa ataaagatgt taatgttacc
     2521 gaagactttt ccttcagtga acctctaccc aacaccacaa atcagcaaag ggtcaaggac
     2581 ttctttaaaa atgctccagc aggacaggaa acacagagag gtggatcaaa atcattattg
     2641 ccagatttct tgcagactcc gaaggaagtt gtatgcacta cccaaaacac accaactgta
     2701 aagaaatccc gggatactgc tctcaagaaa ttagaattta gttcttcacc agattcttta
     2761 agtaccatca atgattggga tgatatggat gactttgata cttctgagac ttcaaaatca
     2821 tttgttacac caccccaaag tcactttgta agagtaagca ctgctcagaa atcaaaaaag
     2881 ggtaagagaa acttttttaa agcacagctt tatacaacaa acacagtaaa gactgatttg
     2941 cctccaccct cctctgaaag cgagcaaata gatttgactg aggaacagaa ggatgactca
     3001 gaatggttaa gcagcgatgt gatttgcatc gatgatggcc ccattgctga agtgcatata
     3061 aatgaagatg ctcaggaaag tgactctctg aaaactcatt tggaagatga aagagataat
     3121 agcgaaaaga agaagaattt ggaagaagct gaattacatt caactgagaa agttccatgt
     3181 attgaatttg atgatgatga ttatgatacg gattttgttc caccttctcc agaagaaatt
     3241 atttctgctt cttcttcctc ttcaaaatgc cttagtacgt taaaggacct tgacacctct
     3301 gacagaaaag aggatgttct tagcacatca aaagatcttt tgtcaaaacc tgagaaaatg
     3361 agtatgcagg agctgaatcc agaaaccagc acagactgtg acgctagaca gataagttta
     3421 cagcagcagc ttattcatgt gatggagcac atctgtaaat taattgatac tattcctgat
     3481 gataaactga aacttttgga ttgtgggaac gaactgcttc agcagcggaa cataagaagg
     3541 aaacttctaa cggaagtaga ttttaataaa agtgatgcca gtcttcttgg ctcattgtgg
     3601 agatacaggc ctgattcact tgatggccct atggagggtg attcctgccc tacagggaat
     3661 tctatgaagg agttaaattt ttcacacctt ccctcaaatt ctgtttctcc tggggactgt
     3721 ttactgacta ccaccctagg aaagacagga ttctctgcca ccaggaagaa tctttttgaa
     3781 aggcctttat tcaataccca tttacagaag tcctttgtaa gtagcaactg ggctgaaaca
     3841 ccaagactag gaaaaaaaaa tgaaagctct tatttcccag gaaatgttct cacaagcact
     3901 gctgtgaaag atcagaataa acatactgct tcaataaatg acttagaaag agaaacccaa
     3961 ccttcctatg atattgataa ttttgacata gatgactttg atgatgatga tgactgggaa
     4021 gacataatgc ataatttagc agccagcaaa tcttccacag ctgcctatca acccatcaag
     4081 gaaggtcggc caattaaatc agtatcagaa agactttcct cagccaagac agactgtctt
     4141 ccagtgtcat ctactgctca aaatataaac ttctcagagt caattcagaa ttatactgac
     4201 aagtcagcac aaaatttagc atccagaaat ctgaaacatg agcgtttcca aagtcttagt
     4261 tttcctcata caaaggaaat gatgaagatt tttcataaaa aatttggcct gcataatttt
     4321 agaactaatc agctagaggc gatcaatgct gcactgcttg gtgaagactg ttttatcctg
     4381 atgccgactg gaggtggtaa gagtttgtgt taccagctcc ctgcctgtgt ttctcctggg
     4441 gtcactgttg tcatttctcc cttgagatca cttatcgtag atcaagtcca aaagctgact
     4501 tccttggata ttccagctac atatctgaca ggtgataaga ctgactcaga agctacaaat
     4561 atttacctcc agttatcaaa aaaagaccca atcataaaac ttctatatgt cactccagaa
     4621 aagatctgtg caagtaacag actcatttct actctggaga atctctatga gaggaagctc
     4681 ttggcacgtt ttgttattga tgaagcacat tgtgtcagtc agtggggaca tgattttcgt
     4741 caagattaca aaagaatgaa tatgcttcgc cagaagtttc cttctgttcc ggtgatggct
     4801 cttacggcca cagctaatcc cagggtacag aaggacatcc tgactcagct gaagattctc
     4861 agacctcagg tgtttagcat gagctttaac agacataatc tgaaatacta tgtattaccg
     4921 aaaaagccta aaaaggtggc atttgattgc ctagaatgga tcagaaagca ccacccatat
     4981 gattcaggga taatttactg cctctccagg cgagaatgtg acaccatggc tgacacgtta
     5041 cagagagatg ggctcgctgc tcttgcttac catgctggcc tcagtgattc tgccagagat
     5101 gaagtgcagc agaagtggat taatcaggat ggctgtcagg ttatctgtgc tacaattgca
     5161 tttggaatgg ggattgacaa accggacgtg cgatttgtga ttcatgcatc tctccctaaa
     5221 tctgtggagg gttactacca agaatctggc agagctggaa gagatgggga aatatctcac
     5281 tgcctgcttt tctataccta tcatgatgtg accagactga aaagacttat aatgatggaa
     5341 aaagatggaa accatcatac aagagaaact cacttcaata atttgtatag catggtacat
     5401 tactgtgaaa atataacgga atgcaggaga atacagcttt tggcctactt tggtgaaaat
     5461 ggatttaatc ctgatttttg taagaaacac ccagatgttt cttgtgataa ttgctgtaaa
     5521 acaaaggatt ataaaacaag agatgtgact gacgatgtga aaagtattgt aagatttgtt
     5581 caagaacata gttcatcaca aggaatgaga aatataaaac atgtaggtcc ttctggaaga
     5641 tttactatga atatgctggt cgacattttc ttggggagta agagtgcaaa aatccagtca
     5701 ggtatatttg gaaaaggatc tgcttattca cgacacaatg ccgaaagact ttttaaaaag
     5761 ctgatacttg acaagatttt ggatgaagac ttatatatca atgccaatga ccaggcgatc
     5821 gcttatgtga tgctcggaaa taaagcccaa actgtactaa atggcaattt aaaggtagac
     5881 tttatggaaa cagaaaattc cagcagtgtg aaaaaacaaa aagcgttagt agcaaaagtg
     5941 tctcagaggg aagagatggt taaaaaatgt cttggagaac ttacagaagt ctgcaaatct
     6001 ctggggaaag tttttggtgt ccattacttc aatattttta ataccgtcac tctcaagaag
     6061 cttgcagaat ctttatcttc tgatcctgag gttttgcttc aaattgatgg tgttactgaa
     6121 gacaaactgg aaaaatatgg tgcggaagtg atttcagtat tacagaaata ctctgaatgg
     6181 acatcgccag ctgaagacag ttccccaggg ataagcctgt ccagcagcag aggccccgga
     6241 agaagtgccg ctgaggagct cgacgaggaa atacccgtat cttcccacta ctttgcaagt
     6301 aaaaccagaa atgaaaggaa gaggaaaaag atgccagcct cccaaaggtc taagaggaga
     6361 aaaactgctt ccagtggttc caaggcaaag ggggggtctg ccacatgtag aaagatatct
     6421 tccaaaacga aatcctccag catcattgga tccagttcag cctcacatac ttctcaagcg
     6481 acatcaggag ccaatagcaa attggggatt atggctccac cgaagcctat aaatagaccg
     6541 tttcttaagc cttcatatgc attctcagga tccgactaca aagaccatga cggtgattat
     6601 aaagatcatg acatcgacta caaggatgac gatgacaagt agcggccgcg aaggatctgc
     6661 gatcgctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg
     6721 gggggagggg tcggcaattg aacgggtgcc tagagaaggt ggcgcggggt aaactgggaa
     6781 agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt
     6841 gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acagctgaag
     6901 cttcgagggg ctcgcatctc tccttcacgc gcccgccgcc ctacctgagg ccgccatcca
     6961 cgccggttga gtcgcgttct gccgcctccc gcctgtggtg cctcctgaac tgcgtccgcc
     7021 gtctaggtaa gtttaaagct caggtcgaga ccgggccttt gtccggcgct cccttggagc
     7081 ctacctagac tcagccggct ctccacgctt tgcctgaccc tgcttgctca actctacgtc
     7141 tttgtttcgt tttctgttct gcgccgttac agatccaagc tgtgaccggc gcctacgcta
     7201 gacgccacca tggagagcga cgagagcggc ctgcccgcca tggagatcga gtgccgcatc
     7261 accggcaccc tgaacggcgt ggagttcgag ctggtgggcg gcggagaggg cacccccaag
     7321 cagggccgca tgaccaacaa gatgaagagc accaaaggcg ccctgacctt cagcccctac
     7381 ctgctgagcc acgtgatggg ctacggcttc taccacttcg gcacctaccc cagcggctac
     7441 gagaacccct tcctgcacgc catcaacaac ggcggctaca ccaacacccg catcgagaag
     7501 tacgaggacg gcggcgtgct gcacgtgagc ttcagctacc gctacgaggc cggccgcgtg
     7561 atcggcgact tcaaggtggt gggcaccggc ttccccgagg acagcgtgat cttcaccgac
     7621 aagatcatcc gcagcaacgc caccgtggag cacctgcacc ccatgggcga taacgtgctg
     7681 gtgggcagct tcgcccgcac cttcagcctg cgcgacggcg gctactacag cttcgtggtg
     7741 gacagccaca tgcacttcaa gagcgccatc caccccagca tcctgcagaa cgggggcccc
     7801 atgttcgcct tccgccgcgt ggaggagctg cacagcaaca ccgagctggg catcgtggag
     7861 taccagcacg ccttcaagac ccccatcgcc ttcgccagat cccgcgctca gtcgtccaat
     7921 tctgccgtgg acggcaccgc cggacccggc tccaccggat ctcgcgaggg cagaggaagt
     7981 cttctaacat gcggtgacgt ggaggagaat cccggcccta tgaccgagta caagcccacg
     8041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     8101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     8161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     8221 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     8281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     8341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     8401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     8461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     8521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     8581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgaa atcaacctct ggattacaaa
     8641 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     8701 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     8761 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     8821 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     8881 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     8941 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     9001 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
     9061 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     9121 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     9181 cggatctccc tttgggccgc ctccccgcct ggtaccttta agaccaatga cttacaaggc
     9241 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
     9301 ccaacgaaga taagatctgc tttttgcttg tactgggtct ctctggttag accagatctg
     9361 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     9421 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     9481 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat
     9541 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc
     9601 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     9661 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct
     9721 ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     9781 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     9841 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagactt ttgcagagac
     9901 caaattcgta atcatgtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     9961 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
    10021 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
    10081 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
    10141 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
    10201 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
    10261 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
    10321 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
    10381 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
    10441 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
    10501 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
    10561 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
    10621 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
    10681 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
    10741 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
    10801 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
    10861 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
    10921 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
    10981 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
    11041 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
    11101 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
    11161 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
    11221 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
    11281 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
    11341 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
    11401 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
    11461 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
    11521 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
    11581 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
    11641 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
    11701 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
    11761 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
    11821 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
    11881 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
    11941 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
    12001 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
    12061 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
    12121 cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
    12181 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
    12241 gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca
    12301 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
    12361 ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt
    12421 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag
    12481 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgccaagct
    12541 g
//
