LOCUS       V031610                11700 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V031610
VERSION     V031610
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..3966
                     /codon_start=1
                     /note="synthetic luc2 version of the luciferase gene"
                     /product="firefly luciferase"
                     /protein_id=""
                     /transl_table=1
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
                     /label="luciferase"
     CDS             4003..5814
                     /label="BIRC3(NM_001165)"
                     /note="BIRC3(NM_001165)"
                     /gene="BIRC3"
     promoter        5859..6070
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6083..6351
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      6380..6389
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6410..7072
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             7142..7195
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             7196..7795
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7796..8383
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8457..8690
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8762..8883
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8902..9037
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(9075..9091)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9099..9115
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9123..9153)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9168..9189
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9477..10065)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10236..11096)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11097..11201)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11675..11691
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg aagatgccaa aaacattaag
     2341 aagggcccag cgccattcta cccactcgaa gacgggaccg ccggcgagca gctgcacaaa
     2401 gccatgaagc gctacgccct ggtgcccggc accatcgcct ttaccgacgc acatatcgag
     2461 gtggacatta cctacgccga gtacttcgag atgagcgttc ggctggcaga agctatgaag
     2521 cgctatgggc tgaatacaaa ccatcggatc gtggtgtgca gcgagaatag cttgcagttc
     2581 ttcatgcccg tgttgggtgc cctgttcatc ggtgtggctg tggccccagc taacgacatc
     2641 tacaacgagc gcgagctgct gaacagcatg ggcatcagcc agcccaccgt cgtattcgtg
     2701 agcaagaaag ggctgcaaaa gatcctcaac gtgcaaaaga agctaccgat catacaaaag
     2761 atcatcatca tggatagcaa gaccgactac cagggcttcc aaagcatgta caccttcgtg
     2821 acttcccatt tgccacccgg cttcaacgag tacgacttcg tgcccgagag cttcgaccgg
     2881 gacaaaacca tcgccctgat catgaacagt agtggcagta ccggattgcc caagggcgta
     2941 gccctaccgc accgcaccgc ttgtgtccga ttcagtcatg cccgcgaccc catcttcggc
     3001 aaccagatca tccccgacac cgctatcctc agcgtggtgc catttcacca cggcttcggc
     3061 atgttcacca cgctgggcta cttgatctgc ggctttcggg tcgtgctcat gtaccgcttc
     3121 gaggaggagc tattcttgcg cagcttgcaa gactataaga ttcaatctgc cctgctggtg
     3181 cccacactat ttagcttctt cgctaagagc actctcatcg acaagtacga cctaagcaac
     3241 ttgcacgaga tcgccagcgg cggggcgccg ctcagcaagg aggtaggtga ggccgtggcc
     3301 aaacgcttcc acctaccagg catccgccag ggctacggcc tgacagaaac aaccagcgcc
     3361 attctgatca cccccgaagg ggacgacaag cctggcgcag taggcaaggt ggtgcccttc
     3421 ttcgaggcta aggtggtgga cttggacacc ggtaagacac tgggtgtgaa ccagcgcggc
     3481 gagctgtgcg tccgtggccc catgatcatg agcggctacg ttaacaaccc cgaggctaca
     3541 aacgctctca tcgacaagga cggctggctg cacagcggcg acatcgccta ctgggacgag
     3601 gacgagcact tcttcatcgt ggaccggctg aagagcctga tcaaatacaa gggctaccag
     3661 gtagccccag ccgaactgga gagcatcctg ctgcaacacc ccaacatctt cgacgccggg
     3721 gtcgccggcc tgcccgacga cgatgccggc gagctgcccg ccgcagtcgt cgtgctggaa
     3781 cacggtaaaa ccatgaccga gaaggagatc gtggactatg tggccagcca ggttacaacc
     3841 gccaagaagc tgcgcggtgg tgttgtgttc gtggacgagg tgcctaaagg actgaccggc
     3901 aagttggacg cccgcaagat ccgcgagatt ctcattaagg ccaagaaggg cggcaagatc
     3961 gccgtgggat ccggtggagg cggatcagga ggcggtggat ctatgaacat agtagaaaac
     4021 agcatattct tatcaaattt gatgaaaagc gccaacacgt ttgaactgaa atacgacttg
     4081 tcatgtgaac tgtaccgaat gtctacgtat tccacttttc ctgctggggt tcctgtctca
     4141 gaaaggagtc ttgctcgtgc tggtttctat tacactggtg tgaatgacaa ggtcaaatgc
     4201 ttctgttgtg gcctgatgct ggataactgg aaaagaggag acagtcctac tgaaaagcat
     4261 aaaaagttgt atcctagctg cagattcgtt cagagtctaa attccgttaa caacttggaa
     4321 gctacctctc agcctacttt tccttcttca gtaacaaatt ccacacactc attacttccg
     4381 ggtacagaaa acagtggata tttccgtggc tcttattcaa actctccatc aaatcctgta
     4441 aactccagag caaatcaaga tttttctgcc ttgatgagaa gttcctacca ctgtgcaatg
     4501 aataacgaaa atgccagatt acttactttt cagacatggc cattgacttt tctgtcgcca
     4561 acagatctgg caaaagcagg cttttactac ataggacctg gagacagagt ggcttgcttt
     4621 gcctgtggtg gaaaattgag caattgggaa ccgaaggata atgctatgtc agaacacctg
     4681 agacattttc ccaaatgccc atttatagaa aatcagcttc aagacacttc aagatacaca
     4741 gtttctaatc tgagcatgca gacacatgca gcccgcttta aaacattctt taactggccc
     4801 tctagtgttc tagttaatcc tgagcagctt gcaagtgcgg gtttttatta tgtgggtaac
     4861 agtgatgatg tcaaatgctt ttgctgtgat ggtggactca ggtgttggga atctggagat
     4921 gatccatggg ttcaacatgc caagtggttt ccaaggtgtg agtacttgat aagaattaaa
     4981 ggacaggagt tcatccgtca agttcaagcc agttaccctc atctacttga acagctgcta
     5041 tccacatcag acagcccagg agatgaaaat gcagagtcat caattatcca ttttgaacct
     5101 ggagaagacc attcagaaga tgcaatcatg atgaatactc ctgtgattaa tgctgccgtg
     5161 gaaatgggct ttagtagaag cctggtaaaa cagacagttc agagaaaaat cctagcaact
     5221 ggagagaatt atagactagt caatgatctt gtgttagact tactcaatgc agaagatgaa
     5281 ataagggaag aggagagaga aagagcaact gaggaaaaag aatcaaatga tttattatta
     5341 atccggaaga atagaatggc actttttcaa catttgactt gtgtaattcc aatcctggat
     5401 agtctactaa ctgccggaat tattaatgaa caagaacatg atgttattaa acagaagaca
     5461 cagacgtctt tacaagcaag agaactgatt gatacgattt tagtaaaagg aaatattgca
     5521 gccactgtat tcagaaactc tctgcaagaa gctgaagctg tgttatatga gcatttattt
     5581 gtgcaacagg acataaaata tattcccaca gaagatgttt cagatctacc agtggaagaa
     5641 caattgcgga gactacaaga agaaagaaca tgtaaagtgt gtatggacaa agaagtgtcc
     5701 atagtgttta ttccttgtgg tcatctagta gtatgcaaag attgtgctcc ttctttaaga
     5761 aagtgtccta tttgtaggag tacaatcaag ggtacagttc gtacatttct ttcatgagcg
     5821 gccgcgaagg atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca catcgcccac
     5881 agtccccgag aagttggggg gaggggtcgg caattgaacg ggtgcctaga gaaggtggcg
     5941 cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg
     6001 agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc
     6061 cagaacacag ctgaagcttc gaggggctcg catctctcct tcacgcgccc gccgccctac
     6121 ctgaggccgc catccacgcc ggttgagtcg cgttctgccg cctcccgcct gtggtgcctc
     6181 ctgaactgcg tccgccgtct aggtaagttt aaagctcagg tcgagaccgg gcctttgtcc
     6241 ggcgctccct tggagcctac ctagactcag ccggctctcc acgctttgcc tgaccctgct
     6301 tgctcaactc tacgtctttg tttcgttttc tgttctgcgc cgttacagat ccaagctgtg
     6361 accggcgcct acgctagacg ccaccatgga gagcgacgag agcggcctgc ccgccatgga
     6421 gatcgagtgc cgcatcaccg gcaccctgaa cggcgtggag ttcgagctgg tgggcggcgg
     6481 agagggcacc cccaagcagg gccgcatgac caacaagatg aagagcacca aaggcgccct
     6541 gaccttcagc ccctacctgc tgagccacgt gatgggctac ggcttctacc acttcggcac
     6601 ctaccccagc ggctacgaga accccttcct gcacgccatc aacaacggcg gctacaccaa
     6661 cacccgcatc gagaagtacg aggacggcgg cgtgctgcac gtgagcttca gctaccgcta
     6721 cgaggccggc cgcgtgatcg gcgacttcaa ggtggtgggc accggcttcc ccgaggacag
     6781 cgtgatcttc accgacaaga tcatccgcag caacgccacc gtggagcacc tgcaccccat
     6841 gggcgataac gtgctggtgg gcagcttcgc ccgcaccttc agcctgcgcg acggcggcta
     6901 ctacagcttc gtggtggaca gccacatgca cttcaagagc gccatccacc ccagcatcct
     6961 gcagaacggg ggccccatgt tcgccttccg ccgcgtggag gagctgcaca gcaacaccga
     7021 gctgggcatc gtggagtacc agcacgcctt caagaccccc atcgccttcg ccagatcccg
     7081 cgctcagtcg tccaattctg ccgtggacgg caccgccgga cccggctcca ccggatctcg
     7141 cgagggcaga ggaagtcttc taacatgcgg tgacgtggag gagaatcccg gccctatgac
     7201 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac
     7261 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca
     7321 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg
     7381 caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt
     7441 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg
     7501 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc
     7561 gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc
     7621 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac
     7681 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt
     7741 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgaaatca
     7801 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     7861 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     7921 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     7981 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     8041 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     8101 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     8161 cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg
     8221 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     8281 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtctccgcct
     8341 tcgccctcag acgagtcgga tctccctttg gccgcctccc cgcctggtac ctttaagacc
     8401 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     8461 agggctaatt cactcccaac gaaaataaga tctgcttttt gcttgtactg ggtctctctg
     8521 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     8581 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     8641 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc
     8701 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     8761 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     8821 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     8881 ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccagttccgc ccattctccg
     8941 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     9001 ctattccaga agtagtgagg aggctttttt ggaggcctag acttttgcag agacggccca
     9061 aattcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     9121 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     9181 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     9241 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     9301 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     9361 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     9421 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     9481 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     9541 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     9601 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     9661 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     9721 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     9781 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     9841 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     9901 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     9961 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
    10021 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
    10081 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
    10141 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
    10201 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
    10261 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
    10321 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
    10381 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
    10441 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
    10501 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
    10561 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
    10621 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
    10681 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
    10741 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
    10801 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
    10861 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
    10921 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
    10981 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
    11041 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
    11101 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
    11161 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
    11221 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
    11281 acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
    11341 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
    11401 ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag
    11461 attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
    11521 taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
    11581 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt
    11641 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgccaagctg
//
