LOCUS V039377 10207 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V039377 VERSION V039377 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1804..1920 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2010..2213 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2302..5061 /label="BCLAF1(NM_014739)" /note="BCLAF1(NM_014739)" /gene="BCLAF1" CDS 5068..5133 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" promoter 5177..5388 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 5401..5669 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" CDS 5696..6295 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 6302..6890 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 6964..7197 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 7269..7390 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 7409..7544 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(7582..7598) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 7606..7622 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(7630..7660) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 7675..7696 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(7984..8572) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(8743..9603) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(9604..9708) /gene="bla" /label="AmpR promoter" primer_bind 10182..10198 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag 2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct 2281 agagctagcg aattcgccac catgggtcgc tccaattcta gatcacattc ttcaaggtca 2341 aagtctagat cacagtctag ttctcgatca agatcaagat ctcattctag aaagaagcga 2401 tacagttcta ggtctcgttc cagaacatat tcaaggtctc gtagtagaga tcgtatgtat 2461 tctagagatt atcgtcgcga ttacagaaat aatagaggaa tgagacgacc ttatgggtac 2521 agaggaaggg gtagagggta ttatcaagga ggaggaggta gatatcatcg aggtggttat 2581 agacctgtct ggaatagaag gcactctagg agtcctagac gaggtcgttc acgttccagg 2641 agtccaaaaa gaagatccgt ttcttctcaa agatccagaa gcagatctcg ccggtcatat 2701 agatcttcta ggtctccaag atcatcctct tctcgttctt catccccata tagcaaatct 2761 cctgtttcta aaagacgagg gtctcaggaa aaacaaacca aaaaagctga aggggaaccc 2821 caagaagaga gtccgttgaa aagtaaatca caggaggaac cgaaagatac atttgaacat 2881 gacccatctg agtctatcga tgaatttaat aagtcatcag ccacatccgg tgatatttgg 2941 cctggccttt cagcttatga taatagtcct agatcacccc atagtccttc acctattgct 3001 acaccaccta gtcagagttc atcttgctct gatgctccca tgctcagtac agttcactct 3061 gcaaaaaata ctccttctca gcattcacat tccattcagc atagtcctga aaggtctggg 3121 tctggttctg ttggaaatgg atctagtcga tacagtcctt ctcagaatag tccaattcat 3181 cacatccctt cacgaagaag tcctgcaaag acaatcgcac cacagaatgc tccaagagat 3241 gagtctaggg gccgttcctc gttttatcct gatggtggag atcaggaaac tgcaaagact 3301 gggaagttct taaaaaggtt cacagatgaa gagtctagag tattcctgct tgataggggt 3361 aataccaggg ataaagaggc ttcaaaagag aaaggatcag agaaagggag ggcagaggga 3421 gaatgggaag atcaggaagc tctagattac ttcagtgata aagagtctgg aaaacaaaag 3481 tttaatgatt cagaagggga tgacacagag gagacagagg attatagaca gttcaggaag 3541 tcagtcctcg cagatcaggg taaaagtttt gctactgcat ctcaccggaa tactgaggag 3601 gaaggactca agtacaagtc caaagtttca ctgaaaggca atagagaaag tgatggattt 3661 agagaagaaa aaaattataa acttaaagag actggatatg tagtggaaag gcctagcact 3721 acaaaagata agcacaaaga agaagacaaa aattctgaaa gaataacagt aaagaaagaa 3781 actcagtcac ctgagcaggt aaagtctgaa aagctcaaag acctctttga ttacagtccc 3841 cctctacaca agaatctgga tgcacgagaa aagtctacct tcagagagga aagcccactt 3901 aggatcaaaa tgatagcgag tgattctcac cgtcctgaag tcaaactcaa aatggcacct 3961 gttcctcttg atgattctaa cagacctgct tccttgacta aagacaggct gcttgctagt 4021 acacttgtcc attctgtcaa gaaggagcaa gaattccgat ccatctttga ccacattaag 4081 ttgccacagg ccagcaaaag cacttcagag tcatttattc aacacattgt gtccttggtt 4141 catcatgtta aagagcaata cttcaagtca gctgcaatga ccctaaacga gcggttcact 4201 tcgtatcaga aagccactga agaacatagt actcggcaaa agagccctga aatacacagg 4261 agaattgaca tctcaccaag taccctgagg aagcataccc gtttagcagg ggaagagaga 4321 gtttttaaag aagaaaatca aaagggagat aaaaaattaa ggtgtgactc tgctgacctt 4381 cggcatgaca ttgatcgccg tagaaaagaa agaagtaaag aacggggaga ttccaagggc 4441 tccagggaat ccagtggatc aagaaagcag gaaaaaactc caaaagatta caaggaatac 4501 aaatcttaca aagatgacag taaacataaa agagagcaag atcattctcg atcttcatcc 4561 tcttcagcat caccttcttc tcccagttct cgagaagaaa aggagagtaa gaaggaaaga 4621 gaagaagaat ttaaaactca ccatgaaatg aaagaatact caggctttgc aggagttagc 4681 cgaccacgag gaaccttttt tcgaattaga ggcagaggaa gagccagagg agtttttgct 4741 gggacaaata ctggtccaaa caactcaaat actacttttc aaaagagacc gaaggaagag 4801 gaatgggatc cagaatatac cccaaagagc aagaagtact tcttgcatga cgacagagat 4861 gatggtgtgg attattgggc caaaagagga agaggtcgtg gtacttttca acgtggcaga 4921 gggcgcttta acttcaaaaa atcaggtagc agtcctaaat ggactcatga caaataccaa 4981 ggggatggga ttgttgaaga tgaagaagag accatggaaa ataatgaaga aaagaaggac 5041 agacgcaagg aagaaaagga aggatccgac tacaaagacc atgacggtga ttataaagat 5101 catgacatcg actacaagga tgacgatgac aagtagcggc cgcgaaggat ctgcgatcgc 5161 tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga 5221 ggggtcggca attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat 5281 gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta 5341 gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga 5401 ggggctcgca tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg 5461 ttgagtcgcg ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag 5521 gtaagtttaa agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct 5581 agactcagcc ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt 5641 tcgttttctg ttctgcgccg ttacagatcc aagctgtgac cggcgcctac gctagatgac 5701 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtcccccggg ccgtacgcac 5761 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgacc cggaccgcca 5821 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg 5881 caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt 5941 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg 6001 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc 6061 gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc 6121 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac 6181 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt 6241 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgagtcga 6301 caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc 6361 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg 6421 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt 6481 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac 6541 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc 6601 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct 6661 gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct 6721 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct 6781 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct 6841 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggtacctt 6901 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg 6961 gactggaagg gctaattcac tcccaacgaa aataagatct gctttttgct tgtactgggt 7021 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc 7081 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg 7141 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta 7201 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga 7261 gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa 7321 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca 7381 atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca gttccgccca 7441 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc 7501 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctagact tttgcagaga 7561 cggcccaaat tcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac 7621 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt 7681 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc 7741 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg 7801 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 7861 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 7921 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 7981 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 8041 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 8101 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 8161 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg 8221 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 8281 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 8341 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 8401 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt 8461 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 8521 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 8581 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 8641 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt 8701 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag 8761 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt 8821 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc 8881 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc 8941 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg 9001 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac 9061 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg 9121 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc 9181 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact 9241 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc 9301 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat 9361 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc 9421 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac 9481 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa 9541 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact 9601 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg 9661 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg 9721 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag 9781 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca 9841 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc 9901 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc 9961 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag 10021 gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg 10081 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg 10141 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtgc 10201 caagctg //