LOCUS       V038651                 9970 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V038651
VERSION     V038651
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..4824
                     /label="BACH2(NM_021813)"
                     /note="BACH2(NM_021813)"
                     /gene="BACH2"
     CDS             4831..4896
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4940..5151
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5164..5432
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5459..6058
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6065..6653
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6727..6960
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7032..7153
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7172..7307
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7345..7361)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7369..7385
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7393..7423)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7438..7459
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7747..8335)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8506..9366)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9367..9471)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     9945..9961
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catgtctgtg gatgagaagc ctgactcccc catgtatgtg
     2341 tatgagtcca cagtccactg caccaacatc ctcctgggcc tcaatgacca gcggaaaaag
     2401 gatattctct gtgacgtgac tttgatcgtg gagaggaagg agttccgggc ccaccgggct
     2461 gtgctggccg catgcagtga atatttttgg caggcgctgg ttggacagac aaaaaatgat
     2521 ttggtggtca gcttgcctga ggaggtcaca gccaggggct ttgggccgct gttacagttt
     2581 gcctacactg ccaagctgtt actcagcaga gaaaacatcc gcgaggtcat ccgctgtgct
     2641 gagttcctgc gcatgcacaa cctggaggac tcctgcttca gcttcctgca gacccagctc
     2701 ctgaacagtg aggatggcct gtttgtgtgc cggaaggatg ctgcgtgcca gcgcccacac
     2761 gaggactgcg agaactctgc aggagaggag gaggatgaag aggaggagac gatggattca
     2821 gagacggcca agatggcttg ccccagggac cagatgcttc cagagcccat cagctttgag
     2881 gccgccgcca tccccgtagc agagaaggaa gaagccctgc tgcccgagcc tgacgtgccc
     2941 acagacacca aggagagctc agaaaaggac gcgttaacgc agtaccccag atacaagaaa
     3001 taccagcttg catgtaccaa gaatgtctat aatgcatcat cacacagtac ctcaggtttt
     3061 gcaagcacat tccgggaaga taactctagc aacagcctca agccggggct tgccaggggg
     3121 cagattaaaa gtgagccgcc cagtgaagag aatgaggaag agagcatcac gctctgcctg
     3181 tctggagatg agcctgacgc caaggacaga gcgggggatg tcgagatgga ccggaaacag
     3241 cccagccctg cccctacccc cacggcccca gctggggccg cctgcctgga gagatccagg
     3301 agcgtggcct cgccctcctg cttaaggtct ctgttcagca taacgaaaag tgtggagctg
     3361 tctggcctgc ccagtacatc tcagcagcac tttgccagga gtccagcctg cccttttgac
     3421 aaggggatca ctcagggtga ccttaaaact gactacaccc ctttcacagg gaattatgga
     3481 cagccccacg tgggccagaa ggaggtgtcc aacttcacca tggggtcgcc cctcaggggg
     3541 cctgggttgg aggctctctg taaacaggag ggagagctgg accggaggag cgtgatcttc
     3601 tcctccagcg cttgtgacca agtgagcacc tcggtgcatt cttattctgg ggtgagcagt
     3661 ttggacaaag acctctctga gccggtgcca aagggtctgt gggtgggagc cggccagtcc
     3721 ctccccagct cgcaggccta ctcccacggt gggctgatgg ccgaccactt gccaggaagg
     3781 atgcggccca acaccagctg cccggtacca atcaaagtct gccctcgctc accccccttg
     3841 gagaccagga ccaggacttc cagctcctgc tcttcctatt cctacgcgga ggacgggagc
     3901 gggggctcac cctgcagcct ccctctctgt gagttctcct cctcgccctg ttcccaggga
     3961 gccagattcc ttgccacaga acatcaggaa ccaggcctga tgggagatgg aatgtacaac
     4021 caagtgcggc cccaaattaa atgtgagcag tcttatggaa ccaactccag tgacgaatcc
     4081 ggatcgttct cggaagcaga cagtgagtcg tgtcctgtgc aggacagggg ccaggaggta
     4141 aaacttcctt ttcctgtaga tcaaatcaca gatcttccaa ggaacgattt ccagatgatg
     4201 attaaaatgc acaagctaac ctcagaacag ttagagttta ttcatgatgt ccgacggcgc
     4261 agcaagaacc gcatcgcggc ccagcgctgc cgcaaaagga aactggactg tattcagaat
     4321 ttagaatgtg aaatccgcaa attggtgtgt gagaaagaga aactgttgtc agagaggaat
     4381 caactgaaag catgcatggg ggaactgttg gacaacttct cctgcctttc ccaggaagtt
     4441 tgccgagaca tccagagccc cgagcagatc caggccctgc atcggtattg ccctgtcctc
     4501 agacccatgg acttgcccac ggcctccagt attaaccctg cgcccttggg tgctgagcag
     4561 aacattgcgg cctcccaatg cgcagtgggg gaaaacgtgc cctgctgctt ggagccaggc
     4621 gcggctcccc ccggaccccc ctgggcaccc agcaacacct ccgagaattg tacctctggg
     4681 aggagactag aaggcactga cccgggaacc ttctcagaga gaggacctcc tcttgaaccc
     4741 aggagccaaa cagtgaccgt ggacttctgc caggaaatga ctgataagtg tacaactgac
     4801 gaacagccca ggaaagatta taccggatcc gactacaaag accatgacgg tgattataaa
     4861 gatcatgaca tcgactacaa ggatgacgat gacaagtagc ggccgcgaag gatctgcgat
     4921 cgctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
     4981 ggaggggtcg gcaattgaac gggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
     5041 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
     5101 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca gctgaagctt
     5161 cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta cctgaggccg ccatccacgc
     5221 cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct cctgaactgc gtccgccgtc
     5281 taggtaagtt taaagctcag gtcgagaccg ggcctttgtc cggcgctccc ttggagccta
     5341 cctagactca gccggctctc cacgctttgc ctgaccctgc ttgctcaact ctacgtcttt
     5401 gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt gaccggcgcc tacgctagat
     5461 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtccccc gggccgtacg
     5521 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg acccggaccg
     5581 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     5641 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
     5701 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     5761 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     5821 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     5881 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     5941 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     6001 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgagt
     6061 cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     6121 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     6181 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     6241 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     6301 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     6361 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     6421 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct
     6481 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     6541 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     6601 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggtac
     6661 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     6721 ggggactgga agggctaatt cactcccaac gaaaataaga tctgcttttt gcttgtactg
     6781 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     6841 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     6901 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     6961 gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
     7021 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     7081 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     7141 tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccagttccgc
     7201 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     7261 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag acttttgcag
     7321 agacggccca aattcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     7381 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     7441 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     7501 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     7561 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     7621 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     7681 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     7741 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     7801 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     7861 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     7921 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     7981 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     8041 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     8101 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     8161 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
     8221 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     8281 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     8341 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     8401 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     8461 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     8521 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     8581 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     8641 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     8701 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     8761 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     8821 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     8881 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     8941 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     9001 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     9061 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     9121 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     9181 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     9241 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     9301 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     9361 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     9421 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     9481 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
     9541 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg
     9601 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
     9661 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc
     9721 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt
     9781 aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg
     9841 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag
     9901 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag
     9961 tgccaagctg
//
