LOCUS       V044052                11407 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V044052
VERSION     V044052
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..5433
                     /label="B4GALNT4(NM_178537)"
                     /note="B4GALNT4(NM_178537)"
                     /gene="B4GALNT4"
     CDS             5440..5505
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5549..5760
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5773..6041
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      6070..6079
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6100..6762
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6832..6885
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6886..7485
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7486..8074
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8148..8381
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8453..8574
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8614..8749
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8782..8798)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8806..8822
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8830..8860)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8875..8896
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9184..9772)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9943..10803)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10804..10908)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11382..11398
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgc cgcggctccc ggtgaagaag
     2341 atccgtaagc agatgaagct gctgctgctg ctgctgctgc tgagctgcgc cgcgtggctc
     2401 acctacgtgc acctgggcct ggtgcgccag ggacgcgcgc tgcgccagcg cctgggctac
     2461 gggcgagatg gtgagaagct gaccagtgag accgacggcc ggggggtcca cgctgcgcca
     2521 tccacacaga gggctgagga ctccagtgag agccgtgaag aggagcaagc gcccgaaggt
     2581 cgggacctag acatgctgtt tcctgggggg gctgggaggc tgccactgaa cttcacccat
     2641 cagacacccc catggcggga ggagtacaag gggcaggtga acctgcacgt gtttgaggac
     2701 tggtgtgggg gcgccgtggg ccacctgagg aggaacctgc acttcccgct gttccctcat
     2761 acgcgcacca ccgtgaagaa gttggccgtg tcccccaagt ggaagaacta tggactccgt
     2821 atttttggtt tcatccaccc ggcgagggac ggagacgtcc agttttctgt ggcctcagac
     2881 gacaactcgg agttctggct gagtctggac gagagccctg ctgctgccca gcttgtggcc
     2941 tttgtgggca agactggctc cgagtggaca gcgcctggag aattcaccaa gttcagctcc
     3001 caggtgtcca agcccaggcg gctcatggcc tcccggaggt actactttga gttgctgcac
     3061 aagcaggacg accgcggctc ggaccacgtg gaagtgggct ggcgagcttt cctgcccggc
     3121 ctgaagttcg aggtcatcag ctctgctcac atctccctgt acacagatga gtcagccttg
     3181 aagatggacc acgtggcgca cgtcccccag tctccagcca gccacgtggg ggggcgtccg
     3241 ccgcaggagg agaccagcgc agacatgctg cggccagatc ccagggatac ctttttcctc
     3301 actccacgca tggaatcttc gagcctggag aacgtgctgg agccctgcgc ctacgccccc
     3361 acctacgtgg tcaaggactt cccgatcgcc agataccagg gcctgcaatt tgtgtacctg
     3421 tccttcgttt atcccaacga ctacactcgc ctcacccaca tggagacgga caacaagtgc
     3481 ttctaccgcg agtctccgct gtatctggag aggtttgggt tctataaata catgaagatg
     3541 gacaaggagg agggggatga ggatgaagaa gacgaggtgc agcgccgagc cttcctcttc
     3601 ctcaacccgg acgacttcct ggacgacgag gacgaggggg agctgctcga cagcctggag
     3661 cccaccgagg cggccccgcc caggagcggc ccccagtccc ccgccccagc agcccccgcc
     3721 cagcccggag ccaccctcgc cccgccgacc cctccccgcc cccgggacgg ggggaccccc
     3781 aggcactccc gggccctgag ctgggccgcc agggccgccc gccctttgcc gctcttcttg
     3841 ggccgagctc cgcccccgcg ccctgcagtg gagcagccgc ccccaaaggt gtacgtgacc
     3901 agggtgcggc cgggacagcg ggcatccccc cgggccccag cgccgcgtgc gccctggccg
     3961 cccttccctg gcgtcttcct gcaccccagg cctctgccca gagtgcagct gcgggcgccc
     4021 ccacgcccac cccggcccca cggccgcagg accggcggcc cccaggccac acagccgagg
     4081 cccccagccc gggcgcaggc cacccaaggg ggccgggagg gccaggcgcg cacgctggga
     4141 cctgcggcgc ccacagtgga ctcaaacttg tcctccgaag cgcggcccgt gacctccttc
     4201 ctgagcttgt cccaggtgtc cgggccgcag ctgcccgggg agggcgaaga ggaggaggaa
     4261 ggggaggacg atggggcccc gggcgacgag gccgcgtcgg aggacagcga ggaggccgcg
     4321 ggcccggcgc tcggacgctg gcgtgaggac gccatcgact ggcagcgcac gttcagcgtg
     4381 ggcgccgtgg acttcgagct gctgcgctcg gactggaacg acctgcgatg caacgtttcg
     4441 gggaacctgc agctgccgga ggcggaggcc gtggacgtga ccgctcagta catggagcgg
     4501 ctgaacgcgc gccacggcgg gcgcttcgcg cttctgcgca tcgtgaacgt ggagaagcgc
     4561 cgggactcgg cgcgagggag tcgcttcctg ctggagctgg agctgcagga gcgcgggggc
     4621 ggccgcctgc gactgtccga gtacgtcttc ctgcggctgc cgggagcccg cgtaggggat
     4681 gcagacggag aaagtcccga acccgctccc gccgcctccg tgcgccccga cggccgcccc
     4741 gagctctgcc ggccactgcg cctggcctgg cgccaggacg tgatggttca cttcatcgtg
     4801 ccagtgaaaa accaggcacg gtgggtggca cagttcctgg cggacatggc tgcgctgcac
     4861 gcgcgcaccg gggactcgcg tttcagcgtc gtcctggtgg atttcgagag cgaggatatg
     4921 gacgtggagc gggccctgcg cgccgcgcgc ctgccccggt accagtacct gagacgaacc
     4981 gggaacttcg agcgctccgc cgggctgcag gcgggagtgg acgcggtaga ggacgccagc
     5041 agcatcgtgt tcctctgcga cctgcacatc cacttcccac ccaacatcct ggacggcatc
     5101 cgcaagcact gcgtggaggg caggctggcc ttcgcgcccg tggtcatgcg cctgagctgc
     5161 gggagctcgc cccgggaccc ccacggttac tgggaggtga acggctttgg cctttttggg
     5221 atctacaagt cggactttga ccgggttgga ggaatgaaca cggaggagtt ccgagaccag
     5281 tgggggggtg aagactggga gctcctggac agggtcctgc aggcagggct ggaggtggag
     5341 cggctccgac tgcggaattt ctatcaccac taccactcca agaggggcat gtggagcgtc
     5401 cgcagcagga agggctctcg cacgggggcg tctggatccg actacaaaga ccatgacggt
     5461 gattataaag atcatgacat cgactacaag gatgacgatg acaagtagcg gccgcgaagg
     5521 atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag
     5581 aagttggggg gaggggtcgg caattgaacg ggtgcctaga gaaggtggcg cggggtaaac
     5641 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat
     5701 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag
     5761 ctgaagcttc gaggggctcg catctctcct tcacgcgccc gccgccctac ctgaggccgc
     5821 catccacgcc ggttgagtcg cgttctgccg cctcccgcct gtggtgcctc ctgaactgcg
     5881 tccgccgtct aggtaagttt aaagctcagg tcgagaccgg gcctttgtcc ggcgctccct
     5941 tggagcctac ctagactcag ccggctctcc acgctttgcc tgaccctgct tgctcaactc
     6001 tacgtctttg tttcgttttc tgttctgcgc cgttacagat ccaagctgtg accggcgcct
     6061 acgctagacg ccaccatgga gagcgacgag agcggcctgc ccgccatgga gatcgagtgc
     6121 cgcatcaccg gcaccctgaa cggcgtggag ttcgagctgg tgggcggcgg agagggcacc
     6181 cccaagcagg gccgcatgac caacaagatg aagagcacca aaggcgccct gaccttcagc
     6241 ccctacctgc tgagccacgt gatgggctac ggcttctacc acttcggcac ctaccccagc
     6301 ggctacgaga accccttcct gcacgccatc aacaacggcg gctacaccaa cacccgcatc
     6361 gagaagtacg aggacggcgg cgtgctgcac gtgagcttca gctaccgcta cgaggccggc
     6421 cgcgtgatcg gcgacttcaa ggtggtgggc accggcttcc ccgaggacag cgtgatcttc
     6481 accgacaaga tcatccgcag caacgccacc gtggagcacc tgcaccccat gggcgataac
     6541 gtgctggtgg gcagcttcgc ccgcaccttc agcctgcgcg acggcggcta ctacagcttc
     6601 gtggtggaca gccacatgca cttcaagagc gccatccacc ccagcatcct gcagaacggg
     6661 ggccccatgt tcgccttccg ccgcgtggag gagctgcaca gcaacaccga gctgggcatc
     6721 gtggagtacc agcacgcctt caagaccccc atcgccttcg ccagatcccg cgctcagtcg
     6781 tccaattctg ccgtggacgg caccgccgga cccggctcca ccggatctcg cgagggcaga
     6841 ggaagtcttc taacatgcgg tgacgtggag gagaatcccg gccctatgac cgagtacaag
     6901 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc
     6961 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg
     7021 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
     7081 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg
     7141 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg
     7201 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg
     7261 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
     7321 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc
     7381 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
     7441 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgaaatca acctctggat
     7501 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     7561 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     7621 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     7681 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     7741 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     7801 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     7861 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc
     7921 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     7981 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     8041 acgagtcgga tctccctttg ggccgcctcc ccgcctggta cctttaagac caatgactta
     8101 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     8161 tcactcccaa cgaagataag atctgctttt tgcttgtact gggtctctct ggttagacca
     8221 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     8281 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     8341 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct
     8401 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt
     8461 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     8521 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     8581 ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     8641 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     8701 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct agacttttgc
     8761 agagaccaaa ttcgtaatca tgtcatagct gtttcctgtg tgaaattgtt atccgctcac
     8821 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     8881 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     8941 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     9001 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     9061 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     9121 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     9181 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     9241 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     9301 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     9361 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     9421 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     9481 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     9541 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     9601 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
     9661 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     9721 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     9781 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     9841 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     9901 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     9961 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
    10021 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
    10081 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
    10141 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
    10201 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
    10261 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
    10321 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
    10381 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
    10441 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
    10501 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
    10561 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
    10621 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
    10681 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
    10741 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
    10801 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
    10861 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
    10921 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
    10981 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca
    11041 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
    11101 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc
    11161 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag
    11221 gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg
    11281 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
    11341 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtgc
    11401 caagctg
//
