LOCUS       V026288                12424 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V026288
VERSION     V026288
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..6450
                     /label="Atrnl1(NM_181415)"
                     /note="Atrnl1(NM_181415)"
                     /gene="Atrnl1"
     CDS             6457..6522
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        6566..6777
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6790..7058
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      7087..7096
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             7117..7779
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             7849..7902
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             7903..8502
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    8503..9091
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             9165..9398
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    9470..9591
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      9631..9766
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(9799..9815)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9823..9839
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9847..9877)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9892..9913
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(10201..10789)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10960..11820)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11821..11925)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     12399..12415
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg agccgggggt ccgggcccgc
     2341 tcgggtgccc cgcagccggc ctccccggtg ctgtggaggg ctcggccggc gggcggtggg
     2401 ggcgcctcct cctggctgct gctggacggg aacagctggc tgctgtgcta tggcttcctc
     2461 tacctggcgc tctatgctca ggtgtcccag tccaagccct gcgagaggac tggctcctgc
     2521 ttctccggtc gctgtgtcaa ctccacctgc ctgtgcgacc cgggctgggt tggggaccag
     2581 tgccagcact gccagggcag gttcaagtta acagaacctt ctggatattt aacagatgga
     2641 ccaattaact ataaatataa aacaaagtgt acatggctaa ttgaaggcta tccaaatgca
     2701 gtgctaaggt taagattcaa tcattttgct acagaatgca gctgggatca tatgtatgtt
     2761 tatgatggag attctatata cgcaccttta gtagctgtac ttagtggctt gatcgttcct
     2821 gaagtgaggg gtaacgagac cgtgcctgag gtggtcacga cgtctggcta cgcgctgctc
     2881 cactttttca gcgatgctgc atataaccta actggcttca acatttttta ttcgatcaat
     2941 tcctgtccta acaactgctc tggtcatgga aagtgtacaa ccagtgtctc tgttgcaagt
     3001 caagtgtatt gtgaatgcga caaatactgg aaaggggaag catgtgacat tccttactgt
     3061 aaagccaatt gtgggagtcc agatcatggc tactgtgacc taacaggaga gaaactctgt
     3121 gtctgcaacg atagttggca aggcccagat tgttctctga atgtcccttc tactgagtct
     3181 tactggattt tgccaaatgt taaacccttc agcccttccg taggtcgggc ctcacataaa
     3241 gcagttttac atgggaaatt catgtgggtg attggaggat atacttttaa ctacagttct
     3301 tttcaaatgg ttctgaatta caatttagaa agcagtatat ggaatgtagg tgctgtatca
     3361 aggggccctc ttcagagata cggacattct cttgccctct atcaggaaaa catctttatg
     3421 tacggaggca gaatggagac cagtgatggc aacgtcaccg atgaattatg ggtgtttaac
     3481 gtacgcagtc aatcatggag cacgaaaacc cccactgtcc ttggccacag tcagcagtac
     3541 gctgtggagg gacactcggc acacatcatg gagctggaca gtagagacgt ggtcatgatt
     3601 gtcatatttg gatattctgc aatatatggc tataccagca gcatacagga ataccatatc
     3661 tcctcaaaca cttggctagt tccagaaacg aaaggagcca ttgtgcaagg tggatatggc
     3721 cacacaagtg tgtatgacga agtgaccaag tccatctatg ttcacggagg ctacaaagca
     3781 ttgcctggca ataagtacgg gctggtggac gacctctata agtacgaagt caacaccagg
     3841 acttggacta ttttgaaaga aagtgggttt gccagatacc ttcactcagc tgttcttatc
     3901 aatggagcta tgcttatttt tggaggaaat acccataatg atacttccct gagtaacggt
     3961 gcaaaatgtt tttctgccga tttcctggca tatgacatag cttgtgacga atggaagaca
     4021 ttgcctaaac caaatctcca tagagatgtc aaccgatttg ggcattctgc agtcgtcatc
     4081 aatgggtcaa tgtatatatt tggtggattt tctagtgtac tccttaatga tatccttgtg
     4141 tataaacccc caaattgcaa agctttccga gatgaagaac tgtgcagaaa cgctggtcca
     4201 gggataaaat gtgtttggaa taagaatcac tgtgaatctt gggagtctgg gaatacaaat
     4261 aatattctca gagccaagtg ccctcccaag acagctgcta ccgatgacag atgttacaga
     4321 tatgctgact gtgccagctg cacagccaac acgaacgggt gccagtggtg tgacgacaag
     4381 aaatgcatct cagccagcag caactgcagc acgtctgtca gaaactacac taaatgtcat
     4441 ataagaaatg agcagatttg taacaaactt acaagctgta aaagctgttc actcaacttg
     4501 aattgccagt gggatcagcg gcagcaggaa tgtcaggctt tgccagctca cctttgtgga
     4561 gaaggctgga atcatgttgg ggacgcttgt cttcgaatca attccagtcg agaaagctat
     4621 gataatgcca aactttattg ctataacctc agtggaaatc tcgcctccct gaccacgtcc
     4681 aaggaggtgg agtttgtgtt ggatgaaata cagaagttca cacagcagaa agtgtcaccg
     4741 tgggtaggcc tacgcaagat caacatatcc tactggggat gggaggacat gtctcctttc
     4801 acaaatacaa gcctgcagtg gcttcctggt gagccaaatg actctggatt ctgtgcctac
     4861 ttagaaaggg ctgcagtggc agggttaaaa gcaaaccctt gcacatccat ggcagatgga
     4921 ctcgtttgtg aaaagcctgt agtaagccca aatcagaacg cgaggccgtg caagaagccg
     4981 tgctccctga ggacctcctg cgccaactgc acgagcagcg gcatggagtg catgtggtgc
     5041 agcagcacga agcgctgcgt ggactccaac gcttacatca tctcctttcc ctacggacag
     5101 tgcctggagt ggcagactgc cacctgctcg cctcaaaatt gttctgggtt aagaacctgt
     5161 ggacagtgct tggagcagcc tgggtgtggc tggtgcaacg atcctagtaa cacaggaaga
     5221 ggctattgca tcgaagggtc ttcccggggc ccaatgaaac tcgcgggggt ccacaacagt
     5281 gacgtggttc ttgacaccag cctctgcccc aaggagaaga actacgagtg gtcttttatc
     5341 cagtgtccag cttgccagtg taatggacac agcacgtgca tcaacaacaa cgtctgtgag
     5401 cagtgtaaga atctcaccac tgggcgacag tgtcaggaat gcatgccagg gtactatgga
     5461 gacccaacca acggtgggca gtgcacagct tgcacgtgcg gcggccatgc gaacgtctgt
     5521 cacctgcaca cgggaaagtg tttttgcaca accaagggga tcaagggtga ccagtgccag
     5581 ctatgtgact ctgaaaatcg ctatgttggt aatccactta gggggacatg ctattacagt
     5641 cttctgattg actaccagtt taccttcagc ttgctgcagg aagatgaccg gcaccacact
     5701 gccatcaact tcatcgccaa cccagagcag tcaaacaaaa acttggacat ttcgattaat
     5761 gcttccaaca actttaatct caacattacg tggtcagttg gctcaacagg tggaaccata
     5821 tctggggaag agactcctat agtttctaag acaaatataa aggaatacag agacagcttt
     5881 tcctatgaaa aatttaactt cagaagcaat cctaatatca cattttatgt gtatgtcagc
     5941 aacttctcct ggcctattaa aatacagatt gcgttttcac aacacaacac aatcatggat
     6001 ctcgtgcagt tctttgtcac cttcttcagt tgttttttat ctttactgct ggtggctgct
     6061 gtggtctgga agatcaaaca aacttgctgg gcttctcgtc ggagggagca actgcttcga
     6121 gaacgacagc agatggccag ccgtcccttt gcttctgttg atgtagccct ggaagtagga
     6181 gctgaacaga cagactttct gcgagggcca ttagagggtg cccctaagcc aatagccatc
     6241 gaaccctgcg ctgggaacag agctgctgtc ctgactgtgt ttctctgtct accgagagga
     6301 tcttcaggcg ccccaccccc tgggcagtca ggccttgcta tcgccagtgc cctgatagac
     6361 atctcacagc agaagccttc tgataataaa gacaagactt ctggagtccg caatcggaag
     6421 cacctctcca cacgtcaagg aacttgtgtc ggatccgact acaaagacca tgacggtgat
     6481 tataaagatc atgacatcga ctacaaggat gacgatgaca agtagcggcc gcgaaggatc
     6541 tgcgatcgct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag
     6601 ttggggggag gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg ggtaaactgg
     6661 gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata
     6721 agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacagctg
     6781 aagcttcgag gggctcgcat ctctccttca cgcgcccgcc gccctacctg aggccgccat
     6841 ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg aactgcgtcc
     6901 gccgtctagg taagtttaaa gctcaggtcg agaccgggcc tttgtccggc gctcccttgg
     6961 agcctaccta gactcagccg gctctccacg ctttgcctga ccctgcttgc tcaactctac
     7021 gtctttgttt cgttttctgt tctgcgccgt tacagatcca agctgtgacc ggcgcctacg
     7081 ctagacgcca ccatggagag cgacgagagc ggcctgcccg ccatggagat cgagtgccgc
     7141 atcaccggca ccctgaacgg cgtggagttc gagctggtgg gcggcggaga gggcaccccc
     7201 aagcagggcc gcatgaccaa caagatgaag agcaccaaag gcgccctgac cttcagcccc
     7261 tacctgctga gccacgtgat gggctacggc ttctaccact tcggcaccta ccccagcggc
     7321 tacgagaacc ccttcctgca cgccatcaac aacggcggct acaccaacac ccgcatcgag
     7381 aagtacgagg acggcggcgt gctgcacgtg agcttcagct accgctacga ggccggccgc
     7441 gtgatcggcg acttcaaggt ggtgggcacc ggcttccccg aggacagcgt gatcttcacc
     7501 gacaagatca tccgcagcaa cgccaccgtg gagcacctgc accccatggg cgataacgtg
     7561 ctggtgggca gcttcgcccg caccttcagc ctgcgcgacg gcggctacta cagcttcgtg
     7621 gtggacagcc acatgcactt caagagcgcc atccacccca gcatcctgca gaacgggggc
     7681 cccatgttcg ccttccgccg cgtggaggag ctgcacagca acaccgagct gggcatcgtg
     7741 gagtaccagc acgccttcaa gacccccatc gccttcgcca gatcccgcgc tcagtcgtcc
     7801 aattctgccg tggacggcac cgccggaccc ggctccaccg gatctcgcga gggcagagga
     7861 agtcttctaa catgcggtga cgtggaggag aatcccggcc ctatgaccga gtacaagccc
     7921 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg
     7981 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc
     8041 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc
     8101 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg
     8161 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag
     8221 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc
     8281 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc
     8341 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc
     8401 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa
     8461 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gaaatcaacc tctggattac
     8521 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     8581 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     8641 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     8701 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     8761 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     8821 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     8881 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
     8941 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     9001 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     9061 agtcggatct ccctttgggc cgcctccccg cctggtacct ttaagaccaa tgacttacaa
     9121 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     9181 ctcccaacga agataagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
     9241 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     9301 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     9361 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat
     9421 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat
     9481 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     9541 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     9601 gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     9661 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     9721 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctaga cttttgcaga
     9781 gaccaaattc gtaatcatgt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     9841 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     9901 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     9961 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
    10021 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
    10081 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
    10141 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
    10201 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
    10261 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
    10321 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
    10381 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
    10441 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
    10501 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
    10561 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
    10621 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
    10681 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
    10741 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
    10801 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
    10861 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
    10921 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
    10981 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
    11041 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
    11101 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
    11161 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
    11221 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
    11281 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
    11341 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
    11401 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
    11461 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
    11521 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
    11581 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
    11641 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
    11701 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
    11761 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
    11821 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
    11881 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
    11941 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
    12001 tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
    12061 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
    12121 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga
    12181 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag
    12241 aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc
    12301 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt
    12361 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa
    12421 gctg
//
