LOCUS       V024275                11852 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V024275
VERSION     V024275
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..5895
                     /label="Atp13a4(NM_001164612)"
                     /note="Atp13a4(NM_001164612)"
                     /gene="Atp13a4"
     CDS             5902..5967
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        6011..6222
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6235..6503
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      6532..6541
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6562..7224
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             7294..7347
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             7348..7947
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7948..8535
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8609..8842
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8914..9035
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      9054..9189
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(9227..9243)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9251..9267
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9275..9305)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9320..9341
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9629..10217)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10388..11248)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11249..11353)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11827..11843
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg gtgaccacct tgagaagagc
     2341 cagcatgctt tactcaatga aggggacgag aatgagatgg aaatatttgg ctatcggact
     2401 caaggctgtc ggaaggctct ttgtctcatt ggatccatct tctctcttgg aatgctccca
     2461 ttggtatttt attggagacc agcttggcgt gtatgggcaa actgtgtccc atgttccctg
     2521 caagaagcag atgttgtgtt gctgaagaca acagatgaat tcaaaattta ttcttggaaa
     2581 aaagtaatat ggatctccct gtcagcactg agcagcacat ctggtctcac gcctgaccac
     2641 cctctcatta cagacgaggg atacatcata aacagagcca tccgaaagcc agatctaaag
     2701 gtgagataca taaaagtgca gaaaatacga tatgtttgga ataatttgga aggacagttt
     2761 cagaaaattg gctccctgga agactggctc agttctgcaa agatacatca gaaatttgga
     2821 ttaggtctga cttcagagga acaagagatc aggaggttaa tatgtgggcc taatgctatt
     2881 gatgttgaaa tcacacctat ttggaaactg ctcatcaagg aggttctgaa tccattttac
     2941 atctttcaac tcttcagcgt ctgtttgtgg ttcagtgaag attacaagga atatgctctt
     3001 gccatcatcc tcatgtctgt catttccata gctttgaccg tgtatgacct cagacagcaa
     3061 tctgtaaaac tgcaccatct tgttgaatcc cataatagca ttacagtctc tgtgtatgag
     3121 cgaaaagctg gagcccaaga cctggaatcc cgcctcctgg tgcctggaga tttattaatt
     3181 ctgacaggga gcagagtgca aatgccgtgt gacgccattc tgattgacgg cagctgtgtg
     3241 gtagatgaag gcatgctgac aggagaaagc attcctgtca ctaaaactcc actatcccag
     3301 acagctagtt ctgtgccctg gaagatgcag tctgaagcag atcccaggcg gcatgttctc
     3361 ttctgtggta cggaggtgat ccaggccaag gcagctggct ctggggctgt aagagccgtg
     3421 gtcctgcaga caggattcaa cactgcaaag ggggacctcg tgagatccat cctgtacccg
     3481 aagcccatga acttcaagct ctacagggat gccatcaggt ttctcctttg ccttgtaggg
     3541 acagccacca ttggcatggt ctatactctg tgtgtctatg tgctcagtgg ggaacctccg
     3601 gaggaggtgg taaggaaagc cctggatgtt attaccattg cagtgcctcc cgctctccct
     3661 gctgccctga ccacaggcat catctatgcc cagaggaggc tgaaaaagaa gggcatattc
     3721 tgcattagcc cccagaggat caatgtttgt ggacagttaa accttgtctg cttcgacaag
     3781 acaggcacct taacgagggg aggcttggat ccttggggag ttgtaccctg tgaccagaat
     3841 ggatttcagg cagtccacag ttttgcctca ggcaaagctt tgcctcaggg tccactgtgt
     3901 gcagccatgg ccagctgcca ctctctgatt ctccttgatg gcactattca gggagaccct
     3961 ctggacctga agatgtttga agccaccaaa tgggaaatga ctgcttctgg ggatgacttc
     4021 catatcaagg aaatgctggc acacaccata gtagtgaagc ctacagacat ggtggcccag
     4081 gtcccagcgg aaggactcgc aatcgtccat cagttcccat tctcatcagc actacagagg
     4141 atgacggtca tcgtccagga gatgggaggt ggccgactgg cgttcatgaa aggtgcaccg
     4201 gagagagtgg ccagcttttg ccaacctgac acagtaccaa ctagttttat tagtgaactt
     4261 cagatttaca caacacaggg tttccgagtc atagctctgg cctacaagaa gctggaaatg
     4321 gactgcccca caactgcctt gatgagggag aaggtggaat cagacctggt atttctggga
     4381 ttgctgatcc tggagaatcg attgaaggaa gagaccaagc ctgtgttaga agagctcatc
     4441 tctgcccgga tacggactgt catgatcaca ggtgacaatc ttcagactgc aataacagtg
     4501 gctaggaagt ctgggatggt ttccgaaggc cagaaagtca ttcttgttga ggcaaatgaa
     4561 gccactggct cttcatcggc atctatctct tggaaattag tggaagagaa gaaacctggt
     4621 ccatttggga gtcaggacac ctatatcaac attagagagg aagtcccaga aaatggcaga
     4681 gacggaagct accattttgc cctgagtgga aaatcctttc atgttataag ccaatatttc
     4741 agcagcttat tgccaaagat actgatcaat gggaccatct ttgcaagaat gtctcctggg
     4801 caaaagtcaa gcctggtgga agaattccag aaactggact actttgtagg tatgtgtgga
     4861 gatggtgcca atgactgtgg ggccctgaag atggctcatg tcggtatctc tttatcagag
     4921 caggaggcat cggtggcctc tcctttcact tccaagactc caaacatcga atgcgtgcca
     4981 catctcatca aggaaggccg cgctgctctc gttacatcct tttgcatgtt taagtacatg
     5041 gccctctaca gcatgattca gtatgttggt gttctgctgc tctactggaa gacaaacagc
     5101 ctttcaaatt accagtttct attccaggat ctggccatca cgactcttat tggtgtaaca
     5161 atgaatctga atggtgccaa ccccaagctc gtgcctttca gacccgcagg gcggctgatc
     5221 tcaccacctc ttctgctctc ggttgtcctc aacatcctgc tcagtttggc catgcacatc
     5281 gtgggcttca tcctggtgca gaagcagcct tggtatatca tggactatca cagtgtctgc
     5341 cctgtgagga atgagagcgc ctcagcctta gctgcatctc ccagcgttcc tgagaaaacc
     5401 agaagtaaca gcacctttgc gagttttgag aatacgacaa tatggttttt gggaacaatc
     5461 aactgtatct ttgtggctct tgtattctct aaaggaaagc catttaggca gcccacctat
     5521 acaaactata tatttgtcct tgtgctgatc ttacagatgg gcgtatgtct tttcattcta
     5581 tttgcggata tcccagagat gcataggcgt ttggacctgc tctgcactcc tgtcctgtgg
     5641 agagtctaca ttctcatcat gatcagctcc aactttgttg tgtcccttgc tgtggagaag
     5701 gccattattg aaaaccgagc tctgtggata gcagtcaaaa gatgttttgg ttatcaatca
     5761 aaaagtcagt atcggatatg gcagaggaac ttggcaaatg attcaagctg gcccccactg
     5821 aaccaaacct cctactctga catgcagggg gtgtcctaca gcaacccggt gtttgagagc
     5881 aatgaggagc aacttggatc cgactacaaa gaccatgacg gtgattataa agatcatgac
     5941 atcgactaca aggatgacga tgacaagtag cggccgcgaa ggatctgcga tcgctccggt
     6001 gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc
     6061 ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg
     6121 tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc
     6181 gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac agctgaagct tcgaggggct
     6241 cgcatctctc cttcacgcgc ccgccgccct acctgaggcc gccatccacg ccggttgagt
     6301 cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg cgtccgccgt ctaggtaagt
     6361 ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc cttggagcct acctagactc
     6421 agccggctct ccacgctttg cctgaccctg cttgctcaac tctacgtctt tgtttcgttt
     6481 tctgttctgc gccgttacag atccaagctg tgaccggcgc ctacgctaga cgccaccatg
     6541 gagagcgacg agagcggcct gcccgccatg gagatcgagt gccgcatcac cggcaccctg
     6601 aacggcgtgg agttcgagct ggtgggcggc ggagagggca cccccaagca gggccgcatg
     6661 accaacaaga tgaagagcac caaaggcgcc ctgaccttca gcccctacct gctgagccac
     6721 gtgatgggct acggcttcta ccacttcggc acctacccca gcggctacga gaaccccttc
     6781 ctgcacgcca tcaacaacgg cggctacacc aacacccgca tcgagaagta cgaggacggc
     6841 ggcgtgctgc acgtgagctt cagctaccgc tacgaggccg gccgcgtgat cggcgacttc
     6901 aaggtggtgg gcaccggctt ccccgaggac agcgtgatct tcaccgacaa gatcatccgc
     6961 agcaacgcca ccgtggagca cctgcacccc atgggcgata acgtgctggt gggcagcttc
     7021 gcccgcacct tcagcctgcg cgacggcggc tactacagct tcgtggtgga cagccacatg
     7081 cacttcaaga gcgccatcca ccccagcatc ctgcagaacg ggggccccat gttcgccttc
     7141 cgccgcgtgg aggagctgca cagcaacacc gagctgggca tcgtggagta ccagcacgcc
     7201 ttcaagaccc ccatcgcctt cgccagatcc cgcgctcagt cgtccaattc tgccgtggac
     7261 ggcaccgccg gacccggctc caccggatct cgcgagggca gaggaagtct tctaacatgc
     7321 ggtgacgtgg aggagaatcc cggccctatg accgagtaca agcccacggt gcgcctcgcc
     7381 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc
     7441 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa
     7501 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc
     7561 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc
     7621 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc
     7681 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg
     7741 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc
     7801 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac
     7861 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg
     7921 tgcatgaccc gcaagcccgg tgcctgaaat caacctctgg attacaaaat ttgtgaaaga
     7981 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     8041 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     8101 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     8161 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     8221 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     8281 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     8341 aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     8401 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     8461 ccggctctgc ggcctcttcc gcgtctccgc cttcgccctc agacgagtcg gatctccctt
     8521 tggccgcctc cccgcctggt acctttaaga ccaatgactt acaaggcagc tgtagatctt
     8581 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaaaataa
     8641 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     8701 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     8761 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     8821 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa
     8881 cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt
     8941 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     9001 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc
     9061 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     9121 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     9181 ttggaggcct agacttttgc agagacggcc caaattcgta atcatggtca tagctgtttc
     9241 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     9301 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     9361 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     9421 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     9481 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     9541 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     9601 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     9661 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     9721 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     9781 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     9841 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     9901 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     9961 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
    10021 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
    10081 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
    10141 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
    10201 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
    10261 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
    10321 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
    10381 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
    10441 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
    10501 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
    10561 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
    10621 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
    10681 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
    10741 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
    10801 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
    10861 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
    10921 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
    10981 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
    11041 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
    11101 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
    11161 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
    11221 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
    11281 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
    11341 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
    11401 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg
    11461 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
    11521 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
    11581 ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
    11641 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca
    11701 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg
    11761 cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac
    11821 gacgttgtaa aacgacggcc agtgccaagc tg
//
