LOCUS       V043399                13678 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V043399
VERSION     V043399
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..3018
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK"
                     /label="EGFP"
     CDS             3055..8532
                     /label="Atad5(NM_001029856)"
                     /note="Atad5(NM_001029856)"
                     /gene="Atad5"
     CDS             8539..8604
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        8648..8859
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             8872..9140
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             9167..9766
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    9773..10361
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             10435..10668
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    10740..10861
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      10880..11015
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(11053..11069)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    11077..11093
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(11101..11131)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    11146..11167
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(11455..12043)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(12214..13074)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(13075..13179)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     13653..13669
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata gaagattcta
     2281 gagctagcga attcagccac catggtgagc aagggcgagg agctgttcac cggggtggtg
     2341 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
     2401 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
     2461 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc
     2521 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     2581 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     2641 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     2701 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
     2761 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
     2821 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     2881 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac
     2941 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     3001 atggacgagc tgtacaaggg aggaggggga agcggtggag gcggttctct cgagatggtg
     3061 ggggtcctga gcatggcggc ggctgcagca ccccttcctg tgaaggacta cgagattgag
     3121 ccatgcaaaa agcggagaaa agatgatgac aactcttcct gcgaaacaat caccaaatat
     3181 ttatcaccaa tagggaagac tggagacaag gttttctctc caccaaaacc cagtaatatt
     3241 ctacattatt ttagaaagac ctcactcaca accgagaagc cacaatcgac aaaggcgtac
     3301 aaaataaagc catctccccc attgcttgtt ggcaacagca aagactgtaa gacgccattg
     3361 gaagtattct caaatagaga atttaagagg aaaagaaaga gagttagttt atcctctcaa
     3421 ctgaatgata ttaaaattca ggatgagtgt ccagttgaaa ttagtaacaa tgatagtaag
     3481 gaggacggtg gtttaagtga ttgtgtggaa agcagtgctt ctgtttccct ttacaaggaa
     3541 catgtggagg ttctcgcaga aagtattgaa gacagcagaa accagccaaa tactaagtcc
     3601 tccaaaaaga aagtaaatcc taaacaatgc accacaaaaa gtgatgacag gatactgagg
     3661 aagaggaagc gtagcaaggt aacgggtcag tctgaaagtg tgcccttagc agatgaactt
     3721 agtctgcctg aggatggtgg caaagacagc aagctgacta aaccttccct agctgaggaa
     3781 aacgactcca ggactcatgc aaccaaaaga gctgatttaa aggagagtac aattactgtc
     3841 tcctatgagg aatttgtaaa aagtcacaag gcagctaaag tggaagagat acctgaccct
     3901 gcagttccag cctgtgttcc ttctgggcca ggtgaagcag tcaaaagtgg ttctgaaggc
     3961 gaattgtcgg gaagctgcga accatcgcca cagctgcacc tcaagacagt tactgttctt
     4021 gcacaagttc accccactcc ccctaagaag aaagggaaaa tacccccaat tttcttgaaa
     4081 cagaagcagc ctgagctgga gaatagtctc tctgacccag aaaatgagca gccagttcag
     4141 aagaggaaat ctaatgttgt gatccaggag ggccaattag agctggcggt gctggaagcc
     4201 tggaactctg aggcttcagt accaaagtgc agcatggagg aaagacagca gttcatgaga
     4261 gcatttaggc agccaccgcc agacacgctc aaaaatggat tcaagaaacc tctggagaag
     4321 cagaaagacc caagtgaaaa atctgtccat gaaggagata gcagttctga aaaaattata
     4381 gaaaatccta atattcaaag agtttcaagc caaggctgtc tacagtcaca tgccgataga
     4441 ggaagttttc ctaaggagaa aagtaaaaag ccaaataaaa agggtaagaa aactaggact
     4501 actgcaggtg gaaacagaga agaaaacata caaaaggaaa aaacagcctt ttccttaaaa
     4561 gatgaacagg atcaaaatag cctacgaagg agtgtaagac agaaatcaga agttctcaaa
     4621 agcaatgcat tacttaacag tgaaaatctt gtttgtgagg atacggcaca tgactctgta
     4681 caaatgtctc tgtgtaatag aaataagtca agaagcagca gcacaccaac cagggatatg
     4741 gttacacatc acagagctga gcctggaagt agcctggagt atgtctctac acgccaacca
     4801 atcaggagat ctctgagaag ttgcagcacg cctgcaacaa atgctctggg aggtactgag
     4861 tctgaggatg ctcaggacac tattccagtg aaggcttcca ccccaaaatc agccagaaca
     4921 tcagaaaagc acaacctgta tacagcagaa ttaataatag tatcttctga ttccgagagc
     4981 cccattagaa tgaaattcac tagaattagt actcccaaaa aatccaagaa aagttctaag
     5041 aaatctgaaa caactgagga agagctcact tctcagaaga aaaaggcaaa tagtacttca
     5101 aaaaatatat caaaagcaaa agaactgatt gaggaagcaa aagcattcca aattggcgga
     5161 tcaaagactg aagaaacagt ggtacccttg aggcggtcct ctaggcacca ggcacgttct
     5221 gcaaaggaga agtcgccaga gatagatgac tctgtaatag taatagactc aagtccaact
     5281 tctataagag aaccagagaa aagtcagaag aaacttcaga atctgaatga tgtactagga
     5341 aaaaaactta acaagtcttc taaaaatgtc cctggaaaaa tgaaaattgc tcctttattt
     5401 cttgccaaaa ggaccaaaag agcagccatt cctgtctttg atttggatga aagcagtcaa
     5461 gattcatcag aacaaactca ggattgtgat gtacagttta aagcaaagcg tgattttcta
     5521 atgagtggtt tgccagattt actgaaacgt cagattgcaa agaaagctgc tgcattagat
     5581 gtgtacaatg cagtgagtac aagtttccag agggttgttc atgtacaaca gaaggatgat
     5641 gaatattggt tatggcattt gaagccacct tcttgtcctc tgttaactga atttaaggaa
     5701 ttaaatacta aagtaacaga tctctcagaa tatgttgttg cttttggtga attttccacc
     5761 ttgaatccaa atccaagaag taatcctgct gctgtgatga tgaggacaag gaaggacttt
     5821 actaaagaag taagaaacct tttgctggag gaaataaagt gctcaaatcc agaattttct
     5881 ttggaaaaat attttccctt gcttcttaaa aaacgaattg aacaccaggt actttgtgag
     5941 ggtcatggta aacaagcaag tccacaacta cagcctgatg tcagtcaaaa agaaaccaaa
     6001 aggaaacaag tggcaacggg aaatcagaag tcaaaaagaa agagacaaaa tgaatattca
     6061 gtgagcccgg aagagatgaa ggggagatca aaagaccttg acgaaagaat cagctcttct
     6121 tgtacaaatc tagatccttc cagagattct ggaactgaag acatgctttg gacagaaaag
     6181 taccaacctc agaattcgaa tgaactcata ggcaatgagt tagctgtcaa aaagttacat
     6241 agttggttga aggactggaa aagaagagct gaactggaag aaagacataa tttgaaagga
     6301 aagagagatg aaaaagagga aggtattttg gatttatcgg atagcacgga ctttaaaggc
     6361 agttcagatg atgaagagaa tcgtctttgc aacactgttc ttataacagg accaacggga
     6421 gtgggaaaaa ctgctgccgt gtatgcttgt gctcaggagc ttgggtttaa gatatttgaa
     6481 gtgaatgcct cttcacagcg aagtggtagg caaatcctgt ctcagctgaa ggaagctacc
     6541 cagtcccatc aagtagataa gcaaggtgta aactcacaaa aaccctgttt ctttaacaac
     6601 tacaacattg gcaagtcacc aaaaaagtta aactccccgg ggaaagttgt tacatcacca
     6661 aggaagcttc ctccatcgtc accaaagaca agtgggcaga agcgagcact tcttcctaaa
     6721 actttggcaa attactttaa agtgtcctcc aaatccaaaa gtaatgacga cgtaggagca
     6781 ctcatgggag atgacaaagg agtcaaaaat tcgtctttgg aacagaggca acttattcag
     6841 actaaatcaa ccaatgcaaa taattcacat attaaagatg ttggagctga agaatccaac
     6901 aggaagaagg cgacatcact cattctcttt gaggaggttg atgtcatttt tgatgaagat
     6961 gctgggtttt tgaatgcagt caaaaccttc atggcaacaa ctaaacgacc agtaattctc
     7021 acgacaagtg atcccacatt tagtctagtg tttgatggct gctttgaaga aatcaatttc
     7081 agtattcctt ccctgctaaa tgttgccagc tatctacaag tgatttgtct agttgagaat
     7141 ttcagaactg actttaaaga ctttgtaacc ttgttgactg caaatgcttg tgacatcaga
     7201 aaaagtatcc tttacttaca gttttggatt agaagtggag gtggaatttt agaagaaagg
     7261 ccattatcac actgtcgaga aaatagcaga aatacactgg tttgttcaga agatggctct
     7321 gatgcaaaca tcaactctaa aaaacccaaa aggaaccgtg tggccctccc caggtgtgac
     7381 actggctgtg cagaggcttt gtttggactc aagaacattg cctccccatc ccaggactta
     7441 ctttcattat tgaagcacaa gatcacaaca aaggaagaat ggcagaaact cattcaggtt
     7501 ctcaccgaat tccacgtgca aaatatcgat ctgctacata gtaaccttga ggtcatcctg
     7561 ccactgccag ttcatgtggt tccagatgta aggggtgctt atggcttccc agtgactaca
     7621 caggccagtg caccagcaag catggggcat cttaccagga agcagtcaaa agaccagcca
     7681 ttgagaaagt cacagaaaag gaaacagaaa aagatggtga tcctagatga cagtgacctg
     7741 tttgacactg gcctagactt ttctggtgaa ctgcctagcc tatctcctgc accctccttg
     7801 tctgtagaag acaacataag aagagacagc aatccagaga ttaagacaca gaacagtggt
     7861 ttcaagcccc actctgtacc tcagccccct aagacactag cagagaagaa gtgttgcatg
     7921 ctcgtttccc actgtttaaa ttctctcagt gagttcatgg agaacatgtc cttcatagat
     7981 gctcttttaa cagatccagg ggaacagaat gaattgggta gaagtgcctt tcactggaca
     8041 aatggaaggg ttaaaagtgg actttgtgat gagtttagtc ttgagaatag agacaggtgg
     8101 gctcctcaga gcgctggaga attaaaagca actgcagaag cactgagctt tactgagtgt
     8161 tcttctacca tttcaaaagc actggagtcc ttgaattcat gcaagcaatt agaaagagac
     8221 ccaaccaatg aactcacagt ctgtgtttca caaaggcgcc atgatgcatg cttccgccaa
     8281 tcagcagcta atctagacaa tgctgacaag aggatggcag ttattaaaag tgtgttttct
     8341 agtcgttctt ttctgaccct gggtaataaa caagctagta taattgacta cctgccaact
     8401 cttcggaaca tttgtcggac agaaaagcta aaagaacaag agaaaaataa aagaaggttt
     8461 ctgcactatt ttgaaggaat tcatctagag attcctgaag aaaccataac tactttggca
     8521 gctgacttcc ctggatccga ctacaaagac catgacggtg attataaaga tcatgacatc
     8581 gactacaagg atgacgatga caagtagcgg ccgcgaagga tctgcgatcg ctccggtgcc
     8641 cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc
     8701 aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac
     8761 tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg
     8821 aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg aggggctcgc
     8881 atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg gttgagtcgc
     8941 gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta ggtaagttta
     9001 aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc tagactcagc
     9061 cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt ttcgttttct
     9121 gttctgcgcc gttacagatc caagctgtga ccggcgccta cgctagatga ccgagtacaa
     9181 gcccacggtg cgcctcgcca cccgcgacga cgtcccccgg gccgtacgca ccctcgccgc
     9241 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgac ccggaccgcc acatcgagcg
     9301 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg
     9361 ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg
     9421 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc
     9481 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct
     9541 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct
     9601 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc
     9661 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc
     9721 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgagtcg acaatcaacc
     9781 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     9841 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     9901 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     9961 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
    10021 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
    10081 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
    10141 tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt
    10201 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
    10261 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
    10321 ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggtacct ttaagaccaa
    10381 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
    10441 ggctaattca ctcccaacga aaataagatc tgctttttgc ttgtactggg tctctctggt
    10501 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
    10561 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
    10621 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat
    10681 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga
    10741 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
    10801 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
    10861 atcatgtctg gctctagcta tcccgcccct aactccgccc agttccgccc attctccgcc
    10921 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
    10981 attccagaag tagtgaggag gcttttttgg aggcctagac ttttgcagag acggcccaaa
    11041 ttcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
    11101 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
    11161 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
    11221 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
    11281 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
    11341 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
    11401 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
    11461 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
    11521 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
    11581 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
    11641 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
    11701 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
    11761 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
    11821 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
    11881 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
    11941 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
    12001 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
    12061 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
    12121 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
    12181 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
    12241 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
    12301 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
    12361 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
    12421 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
    12481 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
    12541 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
    12601 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
    12661 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
    12721 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
    12781 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
    12841 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
    12901 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
    12961 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
    13021 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
    13081 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
    13141 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
    13201 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
    13261 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
    13321 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
    13381 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
    13441 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata
    13501 ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
    13561 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
    13621 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg ccaagctg
//
