LOCUS       V033995                10170 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033995
VERSION     V033995
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2285..3934
                     /EC_number=""
                     /codon_start=1
                     /experiment=""
                     /gene="luc"
                     /product="firefly luciferase"
                     /protein_id=""
                     /transl_table=1
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
                     /label="luciferase"
     CDS             3971..5089
                     /label="Aspn(NM_025711)"
                     /note="Aspn(NM_025711)"
                     /gene="Aspn"
     promoter        5140..5351
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5364..5632
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5659..6258
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6265..6853
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6927..7160
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7232..7353
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7372..7507
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7545..7561)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7569..7585
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7593..7623)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7638..7659
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7947..8535)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8706..9566)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9567..9671)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10145..10161
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattgc
     2281 caccatggaa gacgccaaaa acataaagaa aggcccggcg ccattctatc cgctagagga
     2341 tggaaccgct ggagagcaac tgcataaggc tatgaagaga tacgccctgg ttcctggaac
     2401 aattgctttt acagatgcac atatcgaggt gaacatcacg tacgcggaat acttcgaaat
     2461 gtccgttcgg ttggcagaag ctatgaaacg atatgggctg aatacaaatc acagaatcgt
     2521 cgtatgcagt gaaaactctc ttcaattctt tatgccggtg ttgggcgcgt tatttatcgg
     2581 agttgcagtt gcgcccgcga acgacattta taatgaacgt gaattgctca acagtatgaa
     2641 catttcgcag cctaccgtag tgtttgtttc caaaaagggg ttgcaaaaaa ttttgaacgt
     2701 gcaaaaaaaa ttaccaataa tccagaaaat tattatcatg gattctaaaa cggattacca
     2761 gggatttcag tcgatgtaca cgttcgtcac atctcatcta cctcccggtt ttaatgaata
     2821 cgattttgta ccagagtcct ttgatcgtga caaaacaatt gcactgataa tgaactcctc
     2881 tggatctact gggttaccta agggtgtggc ccttccgcat agaactgcct gcgtcagatt
     2941 ctcgcatgcc agagatccta tttttggcaa tcaaatcatt ccggatactg cgattttaag
     3001 tgttgttcca ttccatcacg gttttggaat gtttactaca ctcggatatt tgatatgtgg
     3061 atttcgagtc gtcttaatgt atagatttga agaagagctg tttttacgat cccttcagga
     3121 ttacaaaatt caaagtgcgt tgctagtacc aaccctattt tcattcttcg ccaaaagcac
     3181 tctgattgac aaatacgatt tatctaattt acacgaaatt gcttctgggg gcgcacctct
     3241 ttcgaaagaa gtcggggaag cggttgcaaa acgcttccat cttccaggga tacgacaagg
     3301 atatgggctc actgagacta catcagctat tctgattaca cccgaggggg atgataaacc
     3361 gggcgcggtc ggtaaagttg ttccattttt tgaagcgaag gttgtggatc tggataccgg
     3421 gaaaacgctg ggcgttaatc agagaggcga attatgtgtc agaggaccta tgattatgtc
     3481 cggttatgta aacaatccgg aagcgaccaa cgccttgatt gacaaggatg gatggctaca
     3541 ttctggagac atagcttact gggacgaaga cgaacacttc ttcatagttg accgcttgaa
     3601 gtctttaatt aaatacaaag gataccaggt ggcccccgct gaattggagt cgatattgtt
     3661 acaacacccc aacatcttcg acgcgggcgt ggcaggtctt cccgacgatg acgccggtga
     3721 acttcccgcc gccgttgttg ttttggagca cggaaagacg atgacggaaa aagagatcgt
     3781 ggattacgtc gccagtcaag taacaaccgc gaaaaagttg cgcggaggag ttgtgtttgt
     3841 ggacgaagta ccgaaaggtc ttaccggaaa actcgacgca agaaaaatca gagagatcct
     3901 cataaaggcc aagaagggcg gaaagtccaa attggaattc ggaggtggtg gatcaggtgg
     3961 aggtggatct atgaaggagt atgtgatgct actgcttttg gctgtgtgct ctgccaaacc
     4021 cttctttagc ccttcccaca cagcactgaa gaatatgatg ttgaaggata tggaagacac
     4081 agatgatgac gataacgatg atgacgacaa ctctcttttt ccaacgaaag agccagtgaa
     4141 cccttttttc cctttcgatt tgtttccaac atgtccattt gggtgccaat gttactctcg
     4201 agttgttcac tgctctgatc taggtctgac atcggttcca aacaacattc catttgatac
     4261 tcgaatggtt gaccttcaaa ataataaaat caaggaaatt aaagaaaatg actttaaagg
     4321 actcacttca ctttatgctc tgattctgaa caacaacaag ctaacaaaga ttcacccaaa
     4381 aacctttcta accacaaaga aattgagaag gctatattta tcccacaacc aactaagtga
     4441 aattccactt aatcttccca aatcattagc agaactcaga attcatgata ataaagttaa
     4501 gaagatacaa aaggacacgt tcaagggaat gaatgcttta catgttttgg aaatgagtgc
     4561 aaaccctctt gagaacaacg ggatagaacc aggggcattt gaaggggtga cagtattcca
     4621 tatcaggatc gctgaagcaa aactaacctc aattccaaaa ggcctaccac caactttgct
     4681 ggagcttcat ttagatttta ataaaatttc aacggtggaa cttgaagatc ttaaacggta
     4741 cagggaactg caaaggctgg gtcttggaaa caacagaatc acagatattg aaaatggaac
     4801 ttttgctaat ataccacgtg tgagagagat acacttggaa cacaataaac taaaaaaaat
     4861 cccttcagga ttacaggagt tgaaatacct ccagataatc ttccttcatt ataattcaat
     4921 tgcaaaagtg ggagtgaatg acttctgtcc aacagtgcca aagatgaaga aatctttata
     4981 cagtgcaata agtttattca acaacccaat gaagtactgg gaaatacaac ctgcaacatt
     5041 tcgttgtgtt cttggcagaa tgagtgttca gcttgggaat gttggaaaat aaggatccgc
     5101 ggccgcgaag gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc acatcgccca
     5161 cagtccccga gaagttgggg ggaggggtcg gcaattgaac gggtgcctag agaaggtggc
     5221 gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg
     5281 gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg
     5341 ccagaacaca gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta
     5401 cctgaggccg ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct
     5461 cctgaactgc gtccgccgtc taggtaagtt taaagctcag gtcgagaccg ggcctttgtc
     5521 cggcgctccc ttggagccta cctagactca gccggctctc cacgctttgc ctgaccctgc
     5581 ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt
     5641 gaccggcgcc tacgctagat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
     5701 gacgtccccc gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
     5761 cacaccgtcg acccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
     5821 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
     5881 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
     5941 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
     6001 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac
     6061 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
     6121 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc
     6181 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
     6241 cgcaagcccg gtgcctgagt cgacaatcaa cctctggatt acaaaatttg tgaaagattg
     6301 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     6361 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     6421 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     6481 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     6541 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     6601 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     6661 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     6721 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     6781 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     6841 gccgcctccc cgcctggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
     6901 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaaaataaga
     6961 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     7021 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     7081 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     7141 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact
     7201 tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta
     7261 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     7321 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc
     7381 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     7441 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     7501 ggaggcctag acttttgcag agacggccca aattcgtaat catggtcata gctgtttcct
     7561 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     7621 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     7681 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     7741 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     7801 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     7861 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     7921 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     7981 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     8041 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     8101 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     8161 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     8221 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     8281 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     8341 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     8401 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     8461 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     8521 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     8581 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     8641 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     8701 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     8761 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     8821 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     8881 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     8941 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     9001 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     9061 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     9121 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     9181 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     9241 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     9301 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     9361 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     9421 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     9481 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     9541 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     9601 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     9661 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     9721 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt
     9781 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
     9841 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
     9901 ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
     9961 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc gccattcagg
    10021 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
    10081 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
    10141 cgttgtaaaa cgacggccag tgccaagctg
//
