LOCUS       V034511                10595 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V034511
VERSION     V034511
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4689
                     /label="Arnt(NM_001037737)"
                     /note="Arnt(NM_001037737)"
                     /gene="Arnt"
     promoter        4754..4965
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4978..5246
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5275..5284
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5305..5967
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6037..6090
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6091..6690
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6691..7278
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7352..7585
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7657..7778
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7797..7932
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7970..7986)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7994..8010
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8018..8048)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8063..8084
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8372..8960)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9131..9991)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9992..10096)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10570..10586
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg cggcgactac agctaaccca
     2341 gaaatgacat cagatgtacc atcgctgggt cccaccattg cttctggaaa ccctggacct
     2401 gggattcaag gtggaggagc tgttgtacag agggctatta agcgacggtc agggctggat
     2461 tttgatgatg aagtagaagt gaacactaaa tttttgagat gcgatgatga ccagatgtgt
     2521 aatgacaaag agcggtttgc caggtcggat gatgagcaga gctctgcgga taaagagaga
     2581 cttgccaggg aaaatcatag tgaaatagaa cggcggcgac ggaacaagat gacagcttac
     2641 atcacagaac tgtcagacat ggtacctaca tgtagtgccc tggctcgaaa accagacaag
     2701 ctaaccatct tacgcatggc cgtttctcac atgaagtcct tgaggggaac tggcaacaca
     2761 tctactgatg gctcctacaa gccatctttc ctcactgatc aggaactgaa acatttgatc
     2821 ttggaggcag cagatggctt tctgtttatt gtctcctgtg agactggacg ggtggtgtat
     2881 gtctctgact cagtgactcc cgttttgaac cagccacagt ctgaatggtt cgggagcaca
     2941 ctgtatgatc aggtgcaccc agatgatgtg gataaacttc gagagcagct ctctacatca
     3001 gaaaatgccc taacagggcg ggtcctggat ctgaagactg gaacagtgaa aaaggaaggc
     3061 cagcagtctt ccatgaggat gtgcatgggc tcacgaaggt cgttcatctg ccgcatgagg
     3121 tgtggtacta gctccgtgga ccctgtttcc atgaatagac tgagcttttt gaggaacaga
     3181 tgcaggaatg ggcttggctc tgtgaaggaa ggagaacctc actttgtggt agtccactgc
     3241 acaggctaca tcaaggcctg gccaccagca ggtgtctccc tcccagatga tgacccagag
     3301 gctggccagg ggagcaaatt ctgcctagtg gccattggca ggctgcaggt aactagttct
     3361 cccaactgta cagacatgag taacatttgt cagccaacag agttcatctc ccgacacaac
     3421 attgaaggga tattcacttt tgtagaccat cgttgtgtgg ctactgttgg ctaccagcca
     3481 caggagctct tagggaagaa tattgtagaa ttttgtcatc ctgaagacca acaacttcta
     3541 agagacagct ttcagcaggt ggtgaaatta aaaggtcagg tgctgtccgt catgttccga
     3601 ttccgatcta agacccgaga atggctgtgg atgagaacga gctcctttac cttccaaaac
     3661 ccttattcag atgaaattga gtatattatc tgcaccaaca ccaatgtgaa gaactctagc
     3721 caggaaccac ggcctacact gtccaacacc atcccaaggt cacagctagg tccgacagcc
     3781 aatttatccc tagagatggg tacagggcag ctgccatcca ggcagcagca gcagcagcac
     3841 acagaactgg atatggtacc aggaagagat gggctggcca gctataatca ttcccaggtt
     3901 tctgtccagc ctgtggcaag tgcaggatca gaacacagca agccccttga gaagtcagaa
     3961 ggtctctttg cacaggacag agatccaagg tttccagaaa tctatcccag catcactgca
     4021 gatcagagta aaggcatctc ctccagcact gtccctgcca cccaacagct gttctcccag
     4081 ggcagctcat tccctcctaa cccccggccg gcagagaatt tcaggaatag tggtcttacc
     4141 cctcctgtaa ccattgtcca gccatcatct tctgcagggc agatactggc ccagatttca
     4201 cgtcactcca accctgccca gggatcagcg ccgacctgga cctctagctc ccgcccaggc
     4261 tttgccgccc agcaggtgcc cacccaggct acagccaaga ctcgttcttc ccaatttggt
     4321 gtgaacaact ttcagacttc ttcctccttc agtgctatgt ctcttccggg tgctcccact
     4381 gcctcatctg gtactgctgc ctaccctgct ctccccaacc gtggctccaa ctttcctcct
     4441 gagactggac agaccacagg acagttccag gcccggacag cagagggcgt gggtgtctgg
     4501 ccacagtggc agggccagca gccccatcat cggtctagtt ccagtgagca gcatgttcag
     4561 cagacacaag cacaagcacc tagccagcct gaggtctttc aagaaatgct gtccatgctg
     4621 ggagaccaaa gcaacaccta caacaatgaa gaatttcctg atctaactat gtttcccccc
     4681 ttttccgaat agggatccct gcgatatcca gcgcggccgc gaaggatctg cgatcgctcc
     4741 ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg
     4801 gtcggcaatt gaacgggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc
     4861 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc
     4921 gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacagctgaa gcttcgaggg
     4981 gctcgcatct ctccttcacg cgcccgccgc cctacctgag gccgccatcc acgccggttg
     5041 agtcgcgttc tgccgcctcc cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta
     5101 agtttaaagc tcaggtcgag accgggcctt tgtccggcgc tcccttggag cctacctaga
     5161 ctcagccggc tctccacgct ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg
     5221 ttttctgttc tgcgccgtta cagatccaag ctgtgaccgg cgcctacgct agacgccacc
     5281 atggagagcg acgagagcgg cctgcccgcc atggagatcg agtgccgcat caccggcacc
     5341 ctgaacggcg tggagttcga gctggtgggc ggcggagagg gcacccccaa gcagggccgc
     5401 atgaccaaca agatgaagag caccaaaggc gccctgacct tcagccccta cctgctgagc
     5461 cacgtgatgg gctacggctt ctaccacttc ggcacctacc ccagcggcta cgagaacccc
     5521 ttcctgcacg ccatcaacaa cggcggctac accaacaccc gcatcgagaa gtacgaggac
     5581 ggcggcgtgc tgcacgtgag cttcagctac cgctacgagg ccggccgcgt gatcggcgac
     5641 ttcaaggtgg tgggcaccgg cttccccgag gacagcgtga tcttcaccga caagatcatc
     5701 cgcagcaacg ccaccgtgga gcacctgcac cccatgggcg ataacgtgct ggtgggcagc
     5761 ttcgcccgca ccttcagcct gcgcgacggc ggctactaca gcttcgtggt ggacagccac
     5821 atgcacttca agagcgccat ccaccccagc atcctgcaga acgggggccc catgttcgcc
     5881 ttccgccgcg tggaggagct gcacagcaac accgagctgg gcatcgtgga gtaccagcac
     5941 gccttcaaga cccccatcgc cttcgccaga tcccgcgctc agtcgtccaa ttctgccgtg
     6001 gacggcaccg ccggacccgg ctccaccgga tctcgcgagg gcagaggaag tcttctaaca
     6061 tgcggtgacg tggaggagaa tcccggccct atgaccgagt acaagcccac ggtgcgcctc
     6121 gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac
     6181 cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa
     6241 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc
     6301 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag
     6361 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa
     6421 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc
     6481 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg
     6541 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc
     6601 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc
     6661 tggtgcatga cccgcaagcc cggtgcctga aatcaacctc tggattacaa aatttgtgaa
     6721 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     6781 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     6841 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     6901 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     6961 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     7021 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     7081 gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg
     7141 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     7201 ctgccggctc tgcggcctct tccgcgtctc cgccttcgcc ctcagacgag tcggatctcc
     7261 ctttggccgc ctccccgcct ggtaccttta agaccaatga cttacaaggc agctgtagat
     7321 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaaa
     7381 taagatctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag
     7441 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     7501 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     7561 tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta
     7621 taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat
     7681 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     7741 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc
     7801 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     7861 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     7921 tttttggagg cctagacttt tgcagagacg gcccaaattc gtaatcatgg tcatagctgt
     7981 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     8041 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     8101 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     8161 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     8221 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     8281 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     8341 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     8401 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     8461 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     8521 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     8581 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     8641 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     8701 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     8761 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
     8821 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     8881 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     8941 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     9001 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     9061 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     9121 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     9181 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     9241 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     9301 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     9361 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     9421 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     9481 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     9541 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     9601 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     9661 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     9721 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     9781 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     9841 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     9901 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     9961 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
    10021 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
    10081 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
    10141 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt
    10201 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
    10261 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
    10321 gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc
    10381 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat
    10441 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc
    10501 tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt
    10561 cacgacgttg taaaacgacg gccagtgcca agctg
//
