LOCUS       V045197                12232 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V045197
VERSION     V045197
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..6258
                     /label="Arid4b(NM_194262)"
                     /note="Arid4b(NM_194262)"
                     /gene="Arid4b"
     CDS             6265..6330
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        6374..6585
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6598..6866
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      6895..6904
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6925..7587
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             7657..7710
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             7711..8310
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    8311..8899
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8973..9206
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    9278..9399
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      9439..9574
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(9607..9623)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9631..9647
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9655..9685)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9700..9721
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(10009..10597)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10768..11628)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11629..11733)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     12207..12223
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatga aggcccttga tgaacctcct
     2341 tatttgacag tgggcactga tgtgagtgcc aaatacagag gagccttctg tgaagccaag
     2401 atcaagactg caaaaagact tgtcaaagtc aaggtgactt ttagacacga ttcttcaaca
     2461 gtggaagttc aggatgacca cataaagggc ccactgaagg taggagctat tgtggaagtg
     2521 aagaatcttg atggtgccta tcaggaagcg gttatcaata aactgacaga tgcaagttgg
     2581 tatactgtgg tttttgatga tggagatgag aagacactga gaagatcttc attatgtctg
     2641 aaaggagaga gacattttgc tgaaagtgaa acactagacc agctcccact caccaatcct
     2701 gagcattttg gcactccagt cataggaaag aaaacaaata gaggaagaag atctaaccat
     2761 ataccagagg aggagtcttc ctcctcatcc agtgatgacg atgaggagga gaggaaacag
     2821 actgatgagc tactaggcaa agttgtttgt gtagattatg ttagcttgga gaaaaagaaa
     2881 gcgatgtggt ttcctgcact ggtggtttgt cctgactgta gtgatgagat tgctgtgaaa
     2941 aaagacaata ttcttgttcg gtctttcaaa gatggcaaat ttacttcagt tccaagaaaa
     3001 gatgtccatg aaattactag tgacactgta ccgaagcctg atgctgtatt aaaacaagcc
     3061 tttgatcagg cacttgagtt tcacaaaagt agagctattc ctgctaattg gaagactgaa
     3121 ttgaaagaag acagctccag tagtgaagct gaggaggagg aggaggagga agatgatgaa
     3181 aaagagaaag aagataacag cagcgaagaa gaggaagaaa tagaaccatt tccagaggaa
     3241 agggagaact ttcttcagca gctgtataag ttcatggagg atagaggtac acctattaac
     3301 aaacggcctg tgcttgggta tcgaaatttg aatcttttta agttattcag acttgtgcac
     3361 aaacttggag ggtttgataa tattgaaagt ggagctgttt ggaagcaagt ctatcaagac
     3421 cttgggatcc ctgtcttaaa ttcagctgca ggatacaatg ttaaatgtgc ttataaaaag
     3481 tacttatatg gctttgagga gtactgcagg tcggctaata ttgactttca gatggcattg
     3541 ccagagaaag ttcttaacaa gccatgtaaa gattgtgaaa ataaagaagt aaaagttaag
     3601 gaagaaagtg aaacagagat taaagaagta aatgtggaag acagtaagaa cgtgatgcca
     3661 aaagaggaga cgcctgctga ggatgaaagt gaaagaaaag aaaatatcaa gccctctctg
     3721 ggaagtaaaa aaagcttatt agaatgtata cctgcacaat ctgatgaaga aaaagaagct
     3781 cacattacaa aactcgaaga aaatgaaaac ttggaagata aagacggtgg cagagctagg
     3841 accgaggagg ccttcagcac agaggtagat ggtgaagaag agcaagcacg atctggagat
     3901 gaaaccaata aagaagaaga tgaagatgat gaagaaatag aagaggagga ggaagaagac
     3961 gaggaagaag acgaggatga agatgatgat gacaacaatg aagaagagga gtttgagtgc
     4021 tatccaccag gcatgaaagt ccaagtgcgg tatggacgag ggaaaaatca aaaaatgtat
     4081 gaagctagta taaaagattc tgatgtcgaa ggtggagagg ccctttactt ggtgcattac
     4141 tgcggatgga atgtgagata tgatgaatgg attaaagcag ataaaatagt aagacctgct
     4201 gataaaaatg taccaaagat caaacatcgg aagaaaataa agaataaact agacaaagaa
     4261 aaagacagag atgaaaaata ctctcctaag aactgtaaac ttcgacgctt gtcaaaatca
     4321 ccgtttcagt caaatccatc tcctgagatg gtttccaaac tagatcttgc tgatgccaaa
     4381 aactctgata cagctcatat taaatctata gaaattactt ccattcttaa tggactacaa
     4441 gcttctgaaa gttcggctga agacagtgag caggaagatg agagatgtac tcaggacgta
     4501 gataacattg gcaaagacga atcaaaggtg gaacattcaa cccacagcag aaatgaactg
     4561 atttcaaaag aggaacagag tagtccatct ttgctagaag aaaacaaagt ccatacagat
     4621 ttggtaatag ccaaaacagt gtcaaaatct ccagaaagat taagaaaaga tatggaagca
     4681 atatcggaag atactgattt tgaagaggaa gatgaaatca caaagaagag aaaggatgtt
     4741 aaaaaggaca caacagacaa ggctttaaag ccacaaacaa aacgtgggaa aagacggtat
     4801 tgcagtgcag atgaatgttt gcagactgga tcccctggca agaaggaaga cagaaccaag
     4861 agtaaagagc cgctctgcac agaaaacagt agtaacagct cctcagatga agatgaagaa
     4921 gaaaagtcca aagcaaagat gacaccaacc aagaaatata atggcttaga agaaaagaga
     4981 aaatctctgc ggacaactag tttctattca ggattttcag aagtagcaga aaaaaggata
     5041 aagcttttaa ataactctga tgaaagactt cagaacaaca gggctaaaga tagaaaagat
     5101 gtctggtcaa gtattcaggg acagtggcct aagaaaacgc taaaggagct tttctctgac
     5161 tcggacactg aggctgcagc ttctccaccc catcctgccc cagatgaggg ggcagtagag
     5221 gagtcactgc agactgtggc agaagaggag agttgttcac ccattatgga gctagaaaag
     5281 ccactacctg ccagtgttga caataagccc attgaagaaa aacccctaga agttagtgat
     5341 aggaaaacag aatttccgag cagtggcagt aattcagtgc tcaatacccc acctaccaca
     5401 ccagagtcgc cttcttcagt cactataaca gaagctagtc agcagcagtc ttctgtcaca
     5461 gtgtcagtgc ccctgcctcc aaaccaggaa gaggttcgaa gcattaagag tgaaactgac
     5521 agcacaattg aggtggacag tgttgtgggg gagctccagg acctccagtc ggaggggaat
     5581 agctcaccag caggctttga tgcgagcgtg agttccagca gtagcaatca gccagaacca
     5641 gataacccag aaaaagcctg tacaggtcag aaaagagtga aagatactca gggagtagga
     5701 agttcatcaa aaaagcagaa gagaagccat aaagccaccg tggtgaacaa caaaaagaag
     5761 ggaaaaggca caaacagtag tgacagtgaa gaactctcag ctggtgaaag tgtgactaag
     5821 actcagacaa tcaagtcagt ccccaccgga atgaagaccc acaacagcaa gtctccagca
     5881 agggtacagt ctccaggaaa aggtggcagg aatggggata aagaccctga tctcaaggag
     5941 cctagtaacc ggcttcccaa ggtttacaag tggagttttc agacgtcgga cctggaaaat
     6001 atgacaagtg ctgaacgtat ctcaattctt caagaaaaat tgcaagaaat cagaaagcat
     6061 tatctgtctt taaaatctga agtagcttcc attgatagga ggagaaaacg tttaaagaag
     6121 aaagagagag aaagtgctgc tacatcttct tcctcctctt ctccctcgtc cagctccatc
     6181 acagctgctg ttatgttgac actagctgaa ccatcaatgt ccagtgcatc acagaatgga
     6241 atgtcagttg agtgcagggg atccgactac aaagaccatg acggtgatta taaagatcat
     6301 gacatcgact acaaggatga cgatgacaag tagcggccgc gaaggatctg cgatcgctcc
     6361 ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg
     6421 gtcggcaatt gaacgggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc
     6481 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc
     6541 gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacagctgaa gcttcgaggg
     6601 gctcgcatct ctccttcacg cgcccgccgc cctacctgag gccgccatcc acgccggttg
     6661 agtcgcgttc tgccgcctcc cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta
     6721 agtttaaagc tcaggtcgag accgggcctt tgtccggcgc tcccttggag cctacctaga
     6781 ctcagccggc tctccacgct ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg
     6841 ttttctgttc tgcgccgtta cagatccaag ctgtgaccgg cgcctacgct agacgccacc
     6901 atggagagcg acgagagcgg cctgcccgcc atggagatcg agtgccgcat caccggcacc
     6961 ctgaacggcg tggagttcga gctggtgggc ggcggagagg gcacccccaa gcagggccgc
     7021 atgaccaaca agatgaagag caccaaaggc gccctgacct tcagccccta cctgctgagc
     7081 cacgtgatgg gctacggctt ctaccacttc ggcacctacc ccagcggcta cgagaacccc
     7141 ttcctgcacg ccatcaacaa cggcggctac accaacaccc gcatcgagaa gtacgaggac
     7201 ggcggcgtgc tgcacgtgag cttcagctac cgctacgagg ccggccgcgt gatcggcgac
     7261 ttcaaggtgg tgggcaccgg cttccccgag gacagcgtga tcttcaccga caagatcatc
     7321 cgcagcaacg ccaccgtgga gcacctgcac cccatgggcg ataacgtgct ggtgggcagc
     7381 ttcgcccgca ccttcagcct gcgcgacggc ggctactaca gcttcgtggt ggacagccac
     7441 atgcacttca agagcgccat ccaccccagc atcctgcaga acgggggccc catgttcgcc
     7501 ttccgccgcg tggaggagct gcacagcaac accgagctgg gcatcgtgga gtaccagcac
     7561 gccttcaaga cccccatcgc cttcgccaga tcccgcgctc agtcgtccaa ttctgccgtg
     7621 gacggcaccg ccggacccgg ctccaccgga tctcgcgagg gcagaggaag tcttctaaca
     7681 tgcggtgacg tggaggagaa tcccggccct atgaccgagt acaagcccac ggtgcgcctc
     7741 gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac
     7801 cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa
     7861 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc
     7921 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag
     7981 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa
     8041 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc
     8101 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg
     8161 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc
     8221 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc
     8281 tggtgcatga cccgcaagcc cggtgcctga aatcaacctc tggattacaa aatttgtgaa
     8341 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     8401 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     8461 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     8521 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     8581 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     8641 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     8701 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
     8761 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     8821 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     8881 ctttgggccg cctccccgcc tggtaccttt aagaccaatg acttacaagg cagctgtaga
     8941 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
     9001 ataagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
     9061 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     9121 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     9181 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt
     9241 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa
     9301 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     9361 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc
     9421 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     9481 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     9541 ttccagaagt agtgaggagg cttttttgga ggcctagact tttgcagaga ccaaattcgt
     9601 aatcatgtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     9661 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     9721 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     9781 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     9841 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     9901 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     9961 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
    10021 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
    10081 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
    10141 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
    10201 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
    10261 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
    10321 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
    10381 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
    10441 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
    10501 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
    10561 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
    10621 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
    10681 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
    10741 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
    10801 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
    10861 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
    10921 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
    10981 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
    11041 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
    11101 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
    11161 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
    11221 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
    11281 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
    11341 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
    11401 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
    11461 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
    11521 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
    11581 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
    11641 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
    11701 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
    11761 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
    11821 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
    11881 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
    11941 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact
    12001 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat
    12061 caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
    12121 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac
    12181 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc tg
//
