LOCUS V024568 10923 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V024568 VERSION V024568 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1804..1921 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2011..2214 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2303..4999 /label="Ar(NM_013476)" /note="Ar(NM_013476)" /gene="Ar" CDS 5012..5038 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" promoter 5082..5293 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 5306..5574 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" regulatory 5603..5612 /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" /regulatory_class="other" /label="Kozak sequence" CDS 5633..6295 /codon_start=1 /product="green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (Shagin et al., 2004)" /transl_table=1 /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA" /label="CopGFP" CDS 6365..6418 /codon_start=1 /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /product="2A peptide from Thosea asigna virus capsid protein" /protein_id="" /transl_table=1 /translation="EGRGSLLTCGDVEENPGP" /label="T2A" CDS 6419..7018 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 7019..7606 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 7680..7913 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 7985..8106 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 8125..8260 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(8298..8314) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 8322..8338 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(8346..8376) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 8391..8412 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(8700..9288) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(9459..10319) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(10320..10424) /gene="bla" /label="AmpR promoter" primer_bind 10898..10914 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc gaattcgcca ccatggaggt gcagttaggg ctgggaaggg tctacccacg 2341 gcccccatcc aagacctatc gaggagcgtt ccagaatctg ttccagagcg tgcgcgaagc 2401 gatccagaac ccgggcccca ggcaccctga ggccgctaac atagcacctc ccggcgcctg 2461 tttacagcag aggcaggaga ctagcccccg gcggcggcgg cggcagcagc acactgagga 2521 tggttctcct caagcccaca tcagaggccc cacaggctac ctggccctgg aggaggaaca 2581 gcagccttca cagcagcagg cagcctccga gggccaccct gagagcagct gcctccccga 2641 gcctggggcg gccaccgctc ctggcaaggg gctgccgcag cagccaccag ctcctccaga 2701 tcaggatgac tcagctgccc catccacgtt gtccctgctg ggccccactt tcccaggctt 2761 aagcagctgc tccgccgaca ttaaagacat tttgaacgag gccggcacca tgcaacttct 2821 tcagcagcag caacaacagc agcagcacca acagcagcac caacagcacc aacagcagca 2881 ggaggtaatc tccgaaggca gcagcgcaag agccagggag gccacggggg ctccctcttc 2941 ctccaaggat agttacctag ggggcaattc aaccatatct gacagtgcca aggagttgtg 3001 taaagcagtg tctgtgtcca tgggattggg tgtggaagca ttggaacatc tgagtccagg 3061 ggaacagctt cggggagact gcatgtacgc gtcgctcctg ggaggtccac ccgcggtgcg 3121 tcccactcct tgtgcgccgc tgcccgaatg caaaggtctt cccctggacg aaggcccagg 3181 caaaagcact gaagagactg ctgagtattc ctctttcaag ggaggttacg ccaaaggatt 3241 ggaaggtgag agcttggggt gctctggcag cagtgaagca ggtagctctg ggacacttga 3301 gatcccgtcc tctctgtctc tgtataaatc tggagcacta gacgaggcag cagcatacca 3361 gaatcgcgac tactacaact ttccgctggc tctgtccggg ccgccgcacc ccccgccccc 3421 tacccatcca cacgcccgta tcaagctgga gaacccattg gactacggca gcgcctgggc 3481 tgcggcggca gcgcaatgcc gctatgggga cttgggtagt ctacatggag ggagtgtagc 3541 cgggcccagc actggatcgc ccccagccac cacctcttct tcctggcata ctctcttcac 3601 agctgaagaa ggccaattat atgggccagg aggcgggggc ggcagcagca gcccaagcga 3661 tgccgggcct gtagccccct atggctacac tcggccccct caggggctga caagccagga 3721 gagtgactac tctgcctccg aagtgtggta tcctggtgga gttgtgaaca gagtacccta 3781 tcccagtccc aattgtgtca aaagtgaaat gggaccttgg atggagaact actccggacc 3841 ttatggggac atgcgtttgg acagtaccag ggaccatgtt ttacccatcg actattactt 3901 tccaccccag aagacctgcc tgatctgtgg agatgaagct tctggctgtc actacggagc 3961 tctcacttgt ggcagctgca aggtcttctt caaaagagcc gctgaaggga aacagaagta 4021 tctatgtgcc agcagaaacg attgtaccat tgataaattt cggaggaaaa attgcccatc 4081 ttgtcgtctc cggaaatgtt atgaagcagg gatgactctg ggagctcgta agctgaagaa 4141 acttggaaat ctaaaactac aggaggaagg agaaaactcc aatgctggca gccccactga 4201 ggacccatcc cagaagatga ctgtatcaca cattgaaggc tatgaatgtc agcctatctt 4261 tcttaacgtc ctggaagcca ttgagccagg agtggtgtgt gccggacatg acaacaacca 4321 accagattcc tttgctgcct tgttatctag cctcaatgag cttggagaga ggcagcttgt 4381 gcatgtggtc aagtgggcca aggccttgcc tggcttccgc aacttgcatg tggatgacca 4441 gatggcggtc attcagtatt cctggatggg actgatggta tttgccatgg gttggcggtc 4501 cttcactaat gtcaactcca ggatgctcta ctttgcacct gacttggttt tcaatgagta 4561 ccgcatgcac aagtctcgga tgtacagcca gtgtgtgagg atgaggcacc tgtctcaaga 4621 gtttggatgg ctccaaataa ccccccagga attcctgtgc atgaaagcac tgctgctctt 4681 cagcattatt ccagtggatg ggctgaaaaa tcaaaaattc tttgatgaac ttcgaatgaa 4741 ctacatcaag gaactcgatc gcatcattgc atgcaaaaga aagaatccca catcctgctc 4801 aaggcgcttc taccagctca ccaagctcct ggattctgtg cagcctattg caagagagct 4861 gcatcagttc acttttgacc tgctaatcaa gtcccatatg gtgagcgtgg actttcctga 4921 aatgatggca gagatcatct ctgtgcaagt gcccaagatc ctttctggga aagtcaagcc 4981 catctatttc cacacacagg gagggagtgg atacccatac gatgttccag attacgctta 5041 gcggccgcga aggatctgcg atcgctccgg tgcccgtcag tgggcagagc gcacatcgcc 5101 cacagtcccc gagaagttgg ggggaggggt cggcaattga acgggtgcct agagaaggtg 5161 gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg 5221 gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc 5281 cgccagaaca cagctgaagc ttcgaggggc tcgcatctct ccttcacgcg cccgccgccc 5341 tacctgaggc cgccatccac gccggttgag tcgcgttctg ccgcctcccg cctgtggtgc 5401 ctcctgaact gcgtccgccg tctaggtaag tttaaagctc aggtcgagac cgggcctttg 5461 tccggcgctc ccttggagcc tacctagact cagccggctc tccacgcttt gcctgaccct 5521 gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg cgccgttaca gatccaagct 5581 gtgaccggcg cctacgctag acgccaccat ggagagcgac gagagcggcc tgcccgccat 5641 ggagatcgag tgccgcatca ccggcaccct gaacggcgtg gagttcgagc tggtgggcgg 5701 cggagagggc acccccaagc agggccgcat gaccaacaag atgaagagca ccaaaggcgc 5761 cctgaccttc agcccctacc tgctgagcca cgtgatgggc tacggcttct accacttcgg 5821 cacctacccc agcggctacg agaacccctt cctgcacgcc atcaacaacg gcggctacac 5881 caacacccgc atcgagaagt acgaggacgg cggcgtgctg cacgtgagct tcagctaccg 5941 ctacgaggcc ggccgcgtga tcggcgactt caaggtggtg ggcaccggct tccccgagga 6001 cagcgtgatc ttcaccgaca agatcatccg cagcaacgcc accgtggagc acctgcaccc 6061 catgggcgat aacgtgctgg tgggcagctt cgcccgcacc ttcagcctgc gcgacggcgg 6121 ctactacagc ttcgtggtgg acagccacat gcacttcaag agcgccatcc accccagcat 6181 cctgcagaac gggggcccca tgttcgcctt ccgccgcgtg gaggagctgc acagcaacac 6241 cgagctgggc atcgtggagt accagcacgc cttcaagacc cccatcgcct tcgccagatc 6301 ccgcgctcag tcgtccaatt ctgccgtgga cggcaccgcc ggacccggct ccaccggatc 6361 tcgcgagggc agaggaagtc ttctaacatg cggtgacgtg gaggagaatc ccggccctat 6421 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg 6481 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg 6541 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat 6601 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag 6661 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc 6721 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc 6781 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag 6841 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga 6901 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga 6961 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgaaa 7021 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc 7081 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat 7141 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg 7201 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg 7261 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat 7321 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt 7381 gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc 7441 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa 7501 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtctccg 7561 ccttcgccct cagacgagtc ggatctccct ttggccgcct ccccgcctgg tacctttaag 7621 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact 7681 ggaagggcta attcactccc aacgaaaata agatctgctt tttgcttgta ctgggtctct 7741 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa 7801 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc 7861 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag 7921 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga 7981 gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt 8041 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt 8101 atcttatcat gtctggctct agctatcccg cccctaactc cgcccagttc cgcccattct 8161 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct 8221 gagctattcc agaagtagtg aggaggcttt tttggaggcc tagacttttg cagagacggc 8281 ccaaattcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt 8341 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc 8401 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc 8461 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct 8521 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca 8581 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac 8641 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt 8701 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg 8761 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc 8821 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc 8881 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc 8941 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac 9001 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt 9061 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct 9121 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc 9181 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt 9241 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg 9301 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc 9361 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa 9421 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag 9481 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg 9541 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga 9601 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag 9661 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa 9721 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc 9781 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca 9841 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg 9901 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat 9961 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc 10021 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg 10081 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg 10141 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt 10201 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca 10261 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata 10321 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac 10381 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa 10441 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt 10501 atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg 10561 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt 10621 cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag 10681 cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga 10741 aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg 10801 gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta 10861 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgccaag 10921 ctg //