LOCUS       V024200                10259 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V024200
VERSION     V024200
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4302
                     /label="Alox12e(NM_145684)"
                     /note="Alox12e(NM_145684)"
                     /gene="Alox12e"
     CDS             4309..4374
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4418..4629
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4642..4910
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      4939..4948
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             4969..5631
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5701..5754
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5755..6354
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6355..6942
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7016..7249
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7321..7442
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7461..7596
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7634..7650)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7658..7674
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7682..7712)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7727..7748
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8036..8624)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8795..9655)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9656..9760)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10234..10250
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg tcaagtacaa gatcctcgta
     2341 gccactggag actcggtttt tgcgggctcc gccaacctgg tgcatctgtg gctggtgggc
     2401 gagcatggag aggcggacct tgggaagcag ctgcggccac tgctgggaag gaagacagag
     2461 ctggaggtcg acgtcccctt gcatctaggg cgcctcctgg cggtgaagct gcgcaaacag
     2521 aaaggcctat tggattctga ctggttctgc aagagcatca ctgtgcaggg ccctgggacc
     2581 caaggagaag cctttttccc ctgctacagt tgggtgcagg gcaaggagac tatctgccta
     2641 accgagggca ctgccctgaa ggtaactgat gacactcaga acctgtttag gaagtatcga
     2701 gagcaggagc tggagaacag aaggaatgtg tatcggtggg gctcctggaa ggagggatta
     2761 atcctgccta tagcagggag tactgaacgg gacctccccc ggaaccagag attcatgaag
     2821 gataaggatt tagatttttc cctctctctg gtcaaagagt tgaagaactt tgctattaag
     2881 gggactctag attttgtaag tcgtgtacaa aagctggaag attaccagaa agtattccca
     2941 catacaaaga ctgctctgcc tgagcgggtc cgtggttcct ggaaggaaga tgctctcttt
     3001 gggtaccagt tcctcaacgg tgcaaacccc atgctcctga ggcggtccat gcgccttcca
     3061 gcccggctgg tcctgcctcc agggatggaa gacgtacaga cccagctgga gaaggagctc
     3121 aaggctggat ctctgttcga agttgatttc tccctgctgg atggagtcaa acctaacatc
     3181 atcattttta agcagcaata tgtgacagcc cctttggtca tgctgaaact acagcccgat
     3241 gggaggctct tacccatggt catccagctc cagccaccac gacacggatg tcccccgcct
     3301 ctgctcttct tgccctcaga tccccccatg gcctggctct tggccaagat ctgggtccga
     3361 agctctgact tccaactgca ccagttacag tcacatctgc tgaggggaca cctcatggcc
     3421 gaggttattt ctgtggccac aatgaggagt ttacccagcc tgcaccccat atacaagctc
     3481 cttgcccccc actttcgata caccatggag atcaacaccc tggcacggaa taatcttgtc
     3541 tccgaatggg gaatttttga cctggtggtg agcacgggga gcggaggcca tgtggacatt
     3601 ctccagagag ccacgtcgtg tttgacctac cgctcctttt gccctccgga tgacttggct
     3661 gaccgtggac tcgtgggggt gaaatcttct ctgtatgccc aggatgccct caggctgtgg
     3721 gagatcatca gtcggtatgt ggaaaggatg gttgagcttt tctacaggag tgacaccgat
     3781 gtgaaggagg acccggagct gcaggtctgg tgccgcgagg tcactgaggt tggactgctt
     3841 ggagcccagg accgagggtt ccccctgtcc ttagagtccc gggctgagct gtgccgcttt
     3901 gtcgctatgt gcatcttcac gtgcactggt cagcacgcat ctacacatct gggtcagctg
     3961 gactggtatg cctggatccc aaatggccca tgcaccatgc ggaagccccc acccatctcc
     4021 aaggatgtga cagagaggga cattgtggac tcgttgcctt gccttcagca ggcacggatg
     4081 cagataacag tcaccaagtt ccttggcaga cgccagcctg tcatggtggc cctggggcag
     4141 cataaagagg agtatttctc aggccccagg ccccgggatg tgctgaagca attccaggag
     4201 gagctggcta tcatggacaa ggagattgag gtccggaatg caagcctgga cctgccctat
     4261 gagtacctcc gacccagcct ggtggaaaac agtgtgacca tcggatccga ctacaaagac
     4321 catgacggtg attataaaga tcatgacatc gactacaagg atgacgatga caagtagcgg
     4381 ccgcgaagga tctgcgatcg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca
     4441 gtccccgaga agttgggggg aggggtcggc aattgaacgg gtgcctagag aaggtggcgc
     4501 ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga
     4561 gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc
     4621 agaacacagc tgaagcttcg aggggctcgc atctctcctt cacgcgcccg ccgccctacc
     4681 tgaggccgcc atccacgccg gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc
     4741 tgaactgcgt ccgccgtcta ggtaagttta aagctcaggt cgagaccggg cctttgtccg
     4801 gcgctccctt ggagcctacc tagactcagc cggctctcca cgctttgcct gaccctgctt
     4861 gctcaactct acgtctttgt ttcgttttct gttctgcgcc gttacagatc caagctgtga
     4921 ccggcgccta cgctagacgc caccatggag agcgacgaga gcggcctgcc cgccatggag
     4981 atcgagtgcc gcatcaccgg caccctgaac ggcgtggagt tcgagctggt gggcggcgga
     5041 gagggcaccc ccaagcaggg ccgcatgacc aacaagatga agagcaccaa aggcgccctg
     5101 accttcagcc cctacctgct gagccacgtg atgggctacg gcttctacca cttcggcacc
     5161 taccccagcg gctacgagaa ccccttcctg cacgccatca acaacggcgg ctacaccaac
     5221 acccgcatcg agaagtacga ggacggcggc gtgctgcacg tgagcttcag ctaccgctac
     5281 gaggccggcc gcgtgatcgg cgacttcaag gtggtgggca ccggcttccc cgaggacagc
     5341 gtgatcttca ccgacaagat catccgcagc aacgccaccg tggagcacct gcaccccatg
     5401 ggcgataacg tgctggtggg cagcttcgcc cgcaccttca gcctgcgcga cggcggctac
     5461 tacagcttcg tggtggacag ccacatgcac ttcaagagcg ccatccaccc cagcatcctg
     5521 cagaacgggg gccccatgtt cgccttccgc cgcgtggagg agctgcacag caacaccgag
     5581 ctgggcatcg tggagtacca gcacgccttc aagaccccca tcgccttcgc cagatcccgc
     5641 gctcagtcgt ccaattctgc cgtggacggc accgccggac ccggctccac cggatctcgc
     5701 gagggcagag gaagtcttct aacatgcggt gacgtggagg agaatcccgg ccctatgacc
     5761 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc
     5821 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac
     5881 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
     5941 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc
     6001 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
     6061 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
     6121 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc
     6181 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc
     6241 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
     6301 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgaaatcaa
     6361 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     6421 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     6481 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     6541 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     6601 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     6661 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     6721 actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt
     6781 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     6841 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tctccgcctt
     6901 cgccctcaga cgagtcggat ctccctttgg ccgcctcccc gcctggtacc tttaagacca
     6961 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
     7021 gggctaattc actcccaacg aaaataagat ctgctttttg cttgtactgg gtctctctgg
     7081 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     7141 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     7201 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca
     7261 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg
     7321 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     7381 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     7441 tatcatgtct ggctctagct atcccgcccc taactccgcc cagttccgcc cattctccgc
     7501 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     7561 tattccagaa gtagtgagga ggcttttttg gaggcctaga cttttgcaga gacggcccaa
     7621 attcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     7681 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     7741 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     7801 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     7861 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     7921 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     7981 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     8041 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     8101 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     8161 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     8221 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     8281 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     8341 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     8401 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     8461 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     8521 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     8581 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     8641 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     8701 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     8761 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     8821 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     8881 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     8941 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     9001 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     9061 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     9121 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     9181 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     9241 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     9301 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     9361 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     9421 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     9481 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     9541 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     9601 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     9661 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     9721 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     9781 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
     9841 cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
     9901 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
     9961 gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga
    10021 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat
    10081 accgcatcag gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
    10141 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
    10201 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gccaagctg
//
