LOCUS       V046657                11692 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V046657
VERSION     V046657
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2303..5734
                     /label="Adcy3(NM_130779)"
                     /note="Adcy3(NM_130779)"
                     /gene="Adcy3"
     CDS             5735..5800
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5851..6062
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6075..6343
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      6372..6381
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6402..7064
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             7134..7187
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             7188..7787
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7788..8375
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8449..8682
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8754..8875
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8894..9029
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(9067..9083)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9091..9107
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9115..9145)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9160..9181
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9469..10057)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10228..11088)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11089..11193)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11667..11683
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc gaattcgcca ccatgccgag gaaccagggc ttctcggatc ccgagtactc
     2341 ggcagagtac tcagccgagt actcagtcag cttgccctct gaccccgacc gcggggtcgg
     2401 ccggacccat gaaatttctg tgcggaactc tgggtcctgc ctgtgcctgc ctcgctttat
     2461 gcggctgacc ttcgtgcctg agtccttgga gaacctctac cagacctact ttaaaaggca
     2521 gcgccatgag acactgctgg tgctggtggt ctttgcggcc ctctttgact gctacgtggt
     2581 agtgatgtgc gcggtggtct tttccagcga caagctggcg cccctcatgg tggcaggcgt
     2641 cggtctggtg ttggacatca tccttttcgt gctctgcaaa aaggggctgc tcccggatcg
     2701 agtgagccgc aaagtggtac cctacctgct gtggctgctc atcacagccc agatcttctc
     2761 ctacctgggc ctgaactttt cgcgtgccca cgcagccagt gacactgtgg gttggcaggc
     2821 cttctttgtc ttctccttct tcataacgct gccactcagc ctcagcccca tcgtgatcat
     2881 ctccgtggtc tcctgtgttg tgcatacgct tgtcttgggg gtcacggtgg cccagcagca
     2941 gcaagacgag ctagaaggga tgcagctgct gagggagatc ctggctaacg tcttcctcta
     3001 cctgtgcgcc atcatcgtgg gcatcatgtc ctactacatg gcagaccgta agcaccgaaa
     3061 ggccttcctg gaggcccgcc agtcgctgga ggtgaagatg aatctggagg agcagagcca
     3121 gcagcaggaa aaccttatgc tttccatcct gccaaagcat gtggctgacg agatgttgaa
     3181 ggacatgaag aaagatgaga gtcagaagga ccagcagcag ttcaacacca tgtacatgta
     3241 ccgccatgag aatgtcagca tcctgtttgc agatattgtg ggctttaccc agctgtcctc
     3301 tgcttgcagt gcccaggagc tcgtgaagct actcaacgag ctctttgccc gctttgacaa
     3361 gctggcggcc aaataccacc agctgaggat caagatccta ggcgactgtt actactgcat
     3421 ctgcggcctg cctgactacc gggaggacca cgccgtgtgc tccatcctga tggggcttgc
     3481 catggtagag gccatctcgt acgtgcggga gaagaccaag accggagtgg acatgcgtgt
     3541 gggggtgcac acaggcactg tgctaggtgg cgtcctgggc cagaagcgct ggcagtatga
     3601 tgtatggtct accgatgtca ctgtggcaaa caagatggag gctggcggca tcccagggcg
     3661 cgtgcacatt tcccagagca ccatggactg cctgaaaggg gagttcgatg tcgaacctgg
     3721 tgatggtggc agtcgctgcg actacctaga tgagaagggc atcgaaacct acctcatcat
     3781 tgcctccaag ccagaggtga agaagacagc tcaaaatggc ctcaacggct cggccctgcc
     3841 aaacggagca ccggcatcca agcccagctc ccctgccctt attgagacca aggagcccaa
     3901 tgggagtgcc catgccagcg gctccacatc agaggaggct gaagaacagg aggcccaggc
     3961 tgacaacccc tcgttcccca acccccgccg caggctgcgc ctccaggacc tggcagaccg
     4021 tgtggtggac gcctctgagg atgagcacga actgaaccag cttcttaacg aggccctgct
     4081 ggagcgggag tccgcccagg tggtaaagaa gagaaacaca ttcctcctaa cgatgaggtt
     4141 catggaccca gagatggaaa cacgctactc ggtggagaag gagaagcaga gtggggctgc
     4201 cttcagctgt tcctgtgtgg tccttttctg cacggccatg gtggagatac ttatcgaccc
     4261 ctggttgatg acaaactacg tgaccttcgt ggttggagag gttctgctct tgatcctgac
     4321 catctgttcg atggctgcca tcttccccag ggcatttcct aagaagctcg tggccttctc
     4381 atcttggatt gaccggaccc gctgggccag aaatacctgg gccatgttag ccatcttcat
     4441 tctggttatg gccaatgttg tggacatgct gagctgtctc cagtactaca tgggacctta
     4501 caacgtgaca accgggatag agctggacgg tggctgtatg gagaacccca agtactacaa
     4561 ctatgttgct gtgctgtccc tcatcgccac catcatgctg gtgcaggtca gccacatggt
     4621 gaagctgaca ctcatgctgc tcgtcacagg cgccgtgact gccatcaacc tgtatgcctg
     4681 gtgtcctgtc tttgatgaat acgaccacaa acgctttcag gaaaaggact ctcctatggt
     4741 ggccttagag aagatgcagg tactttccac ccctgggctc aatggcactg acagcaggct
     4801 gcccctggtg ccttccaagt actcgatgac tgtgatgatg ttcgttatga tgctgagctt
     4861 ttactacttc tcacgccacg tggagaaact ggcccggaca ctgttcttgt ggaagattga
     4921 ggtccatgac cagaaagaac gtgtgtacga gatgcgccgg tggaacgagg ccttggtcac
     4981 caacatgttg ccagagcatg ttgcacgcca tttcctgggc tccaagaaga gagatgagga
     5041 gctgtacagc cagtcttatg acgagattgg agtcatgttt gcctccttgc ccaactttgc
     5101 cgacttctac actgaggaga gcatcaataa tggtggcatc gagtgtctac gcttcctcaa
     5161 tgagatcatc tctgattttg actctctcct ggacaatccc aaattccggg tcatcaccaa
     5221 gatcaaaacc atcggcagca cctatatggc agcttctgga gtcacaccag atgtcaacac
     5281 caatggcttt acaagctcca gcaaggagga aaagtcagac aaggagcgct ggcagcacct
     5341 ggctgacctg gcagactttg cactagccat gaaggacacg cttacaaaca tcaacaacca
     5401 gtcattcaac aacttcatgc tgcgcatagg catgaacaaa ggaggagttc tggctggagt
     5461 cattggagcc cggaagccac actatgacat ctgggggaac acggtcaatg tggccagcag
     5521 gatggaatcc acaggggtca tgggcaatat ccaggtggta gaagagacac aggtcatcct
     5581 tcgagagtat ggcttccgct ttgtgaggcg aggacccatc tttgtgaagg gcaaagggga
     5641 gcttctgacc tttttcttaa aggggcggga caggccagct gccttcccca atggctcctc
     5701 tgttacactg ccccaccaag tggtggacaa ccccgactac aaagaccatg acggtgatta
     5761 taaagatcat gacatcgact acaaggatga cgatgacaag tagggatccg cggccgcgaa
     5821 ggatctgcga tcgctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg
     5881 agaagttggg gggaggggtc ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa
     5941 actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt
     6001 atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac
     6061 agctgaagct tcgaggggct cgcatctctc cttcacgcgc ccgccgccct acctgaggcc
     6121 gccatccacg ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg
     6181 cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc
     6241 cttggagcct acctagactc agccggctct ccacgctttg cctgaccctg cttgctcaac
     6301 tctacgtctt tgtttcgttt tctgttctgc gccgttacag atccaagctg tgaccggcgc
     6361 ctacgctaga cgccaccatg gagagcgacg agagcggcct gcccgccatg gagatcgagt
     6421 gccgcatcac cggcaccctg aacggcgtgg agttcgagct ggtgggcggc ggagagggca
     6481 cccccaagca gggccgcatg accaacaaga tgaagagcac caaaggcgcc ctgaccttca
     6541 gcccctacct gctgagccac gtgatgggct acggcttcta ccacttcggc acctacccca
     6601 gcggctacga gaaccccttc ctgcacgcca tcaacaacgg cggctacacc aacacccgca
     6661 tcgagaagta cgaggacggc ggcgtgctgc acgtgagctt cagctaccgc tacgaggccg
     6721 gccgcgtgat cggcgacttc aaggtggtgg gcaccggctt ccccgaggac agcgtgatct
     6781 tcaccgacaa gatcatccgc agcaacgcca ccgtggagca cctgcacccc atgggcgata
     6841 acgtgctggt gggcagcttc gcccgcacct tcagcctgcg cgacggcggc tactacagct
     6901 tcgtggtgga cagccacatg cacttcaaga gcgccatcca ccccagcatc ctgcagaacg
     6961 ggggccccat gttcgccttc cgccgcgtgg aggagctgca cagcaacacc gagctgggca
     7021 tcgtggagta ccagcacgcc ttcaagaccc ccatcgcctt cgccagatcc cgcgctcagt
     7081 cgtccaattc tgccgtggac ggcaccgccg gacccggctc caccggatct cgcgagggca
     7141 gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc cggccctatg accgagtaca
     7201 agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg
     7261 ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc
     7321 gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt
     7381 gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg
     7441 gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg
     7501 cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc
     7561 tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc
     7621 tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc
     7681 cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc
     7741 ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgaaat caacctctgg
     7801 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     7861 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     7921 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     7981 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     8041 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     8101 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     8161 attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca
     8221 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     8281 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtctccgc cttcgccctc
     8341 agacgagtcg gatctccctt tggccgcctc cccgcctggt acctttaaga ccaatgactt
     8401 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     8461 ttcactccca acgaaaataa gatctgcttt ttgcttgtac tgggtctctc tggttagacc
     8521 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     8581 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     8641 gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc
     8701 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt
     8761 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     8821 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     8881 tctggctcta gctatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     8941 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     9001 gaagtagtga ggaggctttt ttggaggcct agacttttgc agagacggcc caaattcgta
     9061 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     9121 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     9181 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     9241 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     9301 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     9361 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     9421 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     9481 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     9541 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     9601 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     9661 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     9721 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     9781 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     9841 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     9901 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     9961 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
    10021 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
    10081 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
    10141 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
    10201 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
    10261 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
    10321 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
    10381 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
    10441 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
    10501 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
    10561 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
    10621 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
    10681 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
    10741 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
    10801 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
    10861 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
    10921 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
    10981 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
    11041 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
    11101 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
    11161 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
    11221 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
    11281 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
    11341 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
    11401 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact
    11461 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat
    11521 caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
    11581 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac
    11641 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc tg
//
