LOCUS       V046303                10879 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V046303
VERSION     V046303
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4905
                     /label="Adam9(NM_001270996)"
                     /note="Adam9(NM_001270996)"
                     /gene="Adam9"
     CDS             4912..4977
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5021..5232
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5245..5513
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5542..5551
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5572..6234
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6304..6357
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6358..6957
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6958..7546
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7620..7853
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7925..8046
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8086..8221
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8254..8270)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8278..8294
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8302..8332)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8347..8368
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8656..9244)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9415..10275)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10276..10380)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10854..10870
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg ggccgcgcgc gctctcgccc
     2341 cttgcctctc tgcgactaag gtggctgctg gcgtgtggct tgctgggccc agtcctcgag
     2401 gccgggcgac cagacttgga acagactgtc catctttctt cttatgaaat tattactcct
     2461 tggagattaa ctagagaaag aagggaagct ctggggccca gttcacagca gatctcttac
     2521 gtcatccagg cccaaggaaa acagcatatt attcacttgg aaagaaacac agacctttta
     2581 cctaatgatt ttgtagttta cacctacgac aaggaaggct ccctactctc tgaccatccc
     2641 aacgtacaga gccattgtca ctatcgaggc tatgtggagg gagtgcagaa ttccgcggtt
     2701 gctgtgagcg cctgctttgg actcagaggc ttgctgcatt tggagaatgc cagttttgga
     2761 attgaacctc tgcacaacag ctcacacttt gagcacatat tttaccccat ggatggcatc
     2821 caccaggagc ctctgagatg tggagtctct aacagggaca cagagaagga aggcacacag
     2881 ggggatgagg aggagcatcc gagtgtcact cagctgctgc gcagaagaag agctgttcta
     2941 ccacagaccc gctatgtgga gctgttcatt gttgtagaca aggaaaggta cgacatgatg
     3001 ggacggaacc agactgctgt gagagaagag atgattcgct tagcaaacta cctggatagc
     3061 atgtacatca tgttaaacat tcgaattgtg ctggttggac tagaaatttg gacagacaga
     3121 aatcctatca atataattgg aggagctgga gatgtgctgg gcaactttgt tcagtggcgg
     3181 gaaaagttcc ttataactcg ttggagacac gacagtgcac agttggtttt gaagaaaggc
     3241 tttggtggaa ctgcaggaat ggcgtttgta ggaacagtat gttcaaggag ccacgcaggt
     3301 gggatcaatg tgtttgggca aatcactgtg gagacatttg catccattgt tgctcatgaa
     3361 ttggggcata accttggaat gaatcatgat gatgggagag agtgtttctg tggagcaaag
     3421 agctgtatca tgaattcagg agcatccggg tccagaaact ttagcagttg cagtgcggag
     3481 gactttgaga agttaacgtt gaataaggga ggaagctgcc tgcttaacat cccgaagccc
     3541 gacgaagcct acagcgcgcc ctcctgtggt aataagctgg tggaccctgg agaggagtgt
     3601 gactgcggca cagcgaagga gtgtgaggtg gacccatgct gtgaaggaag cacttgtaag
     3661 ctcaagtcat ttgctgagtg tgcatatggc gactgttgta aagattgcca gttccttcca
     3721 ggaggctcca tgtgcagagg gaagaccagt gagtgtgatg ttcctgagta ctgcaacggt
     3781 tcctctcagt tctgcccgcc agatgtcttc attcagaatg gatatccttg ccagaacagc
     3841 aaagcctact gctacaatgg catgtgccaa tattatgacg cgcagtgtca ggtcatcttt
     3901 ggttcaaagg ctaaggctgc cccaagagat tgcttcattg aagtcaattc taaaggtgac
     3961 agatttggca actgtggttt ctccggcagt gagtacaaga agtgtgccac tgggaacgcg
     4021 ctgtgtggaa agcttcaatg cgagaatgta caggacatgc cggtgtttgg aatagtacca
     4081 gctatcattc agacacccag tcgaggcacc aaatgctggg gtgtggattt ccagcttggt
     4141 tccgacgttc cagacccagg gatggtgaat gaaggcacca aatgtgatgc tggcaagatt
     4201 tgcaggaatt ttcagtgtgt aaatgcttct gtcctgaatt atgactgtga cattcaggga
     4261 aaatgtcatg gccatggggt gtgtaacagc aataagaatt gtcactgtga agacggctgg
     4321 gctcccccac actgtgacac caaaggatat ggaggaagcg tggacagcgg gccgacgtat
     4381 aatgcaaaga gcacagcact gagggacggg cttctggtct tcttcttcct aatcgtcccc
     4441 cttgttgcgg ctgccatttt cctctttatc aagagagatg aactacggaa aaccttcagg
     4501 aagaagagat cacaaatgtc agatggcaga aatcaagcaa acgtctctag acagccagga
     4561 gatcctagta tctccagacc accagggggc ccaaatgtct ccagaccacc agggggccca
     4621 ggtgtctcca gaccaccagg gggcccaggt gtctccagac caccaggggg cccaggtgtc
     4681 tccagaccac cagggggccc aggtgtctcc agaccgccag ggggcccagg tgtctccaga
     4741 ccgccacctg ggcatggaaa cagattccca gtaccaacct acgccgccaa gcagcctgcg
     4801 cagttcccgt caaggccacc tccaccacaa ccgaaaatat cttctcaggg aaacttgatt
     4861 ccggctcggc ccgctcctgc acctccttta tatagctccc tcaccggatc cgactacaaa
     4921 gaccatgacg gtgattataa agatcatgac atcgactaca aggatgacga tgacaagtag
     4981 cggccgcgaa ggatctgcga tcgctccggt gcccgtcagt gggcagagcg cacatcgccc
     5041 acagtccccg agaagttggg gggaggggtc ggcaattgaa cgggtgccta gagaaggtgg
     5101 cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg
     5161 ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc
     5221 gccagaacac agctgaagct tcgaggggct cgcatctctc cttcacgcgc ccgccgccct
     5281 acctgaggcc gccatccacg ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc
     5341 tcctgaactg cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt
     5401 ccggcgctcc cttggagcct acctagactc agccggctct ccacgctttg cctgaccctg
     5461 cttgctcaac tctacgtctt tgtttcgttt tctgttctgc gccgttacag atccaagctg
     5521 tgaccggcgc ctacgctaga cgccaccatg gagagcgacg agagcggcct gcccgccatg
     5581 gagatcgagt gccgcatcac cggcaccctg aacggcgtgg agttcgagct ggtgggcggc
     5641 ggagagggca cccccaagca gggccgcatg accaacaaga tgaagagcac caaaggcgcc
     5701 ctgaccttca gcccctacct gctgagccac gtgatgggct acggcttcta ccacttcggc
     5761 acctacccca gcggctacga gaaccccttc ctgcacgcca tcaacaacgg cggctacacc
     5821 aacacccgca tcgagaagta cgaggacggc ggcgtgctgc acgtgagctt cagctaccgc
     5881 tacgaggccg gccgcgtgat cggcgacttc aaggtggtgg gcaccggctt ccccgaggac
     5941 agcgtgatct tcaccgacaa gatcatccgc agcaacgcca ccgtggagca cctgcacccc
     6001 atgggcgata acgtgctggt gggcagcttc gcccgcacct tcagcctgcg cgacggcggc
     6061 tactacagct tcgtggtgga cagccacatg cacttcaaga gcgccatcca ccccagcatc
     6121 ctgcagaacg ggggccccat gttcgccttc cgccgcgtgg aggagctgca cagcaacacc
     6181 gagctgggca tcgtggagta ccagcacgcc ttcaagaccc ccatcgcctt cgccagatcc
     6241 cgcgctcagt cgtccaattc tgccgtggac ggcaccgccg gacccggctc caccggatct
     6301 cgcgagggca gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc cggccctatg
     6361 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc
     6421 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc
     6481 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc
     6541 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc
     6601 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc
     6661 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc
     6721 gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc
     6781 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag
     6841 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac
     6901 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgaaat
     6961 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     7021 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     7081 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     7141 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     7201 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     7261 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     7321 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
     7381 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     7441 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     7501 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg tacctttaag
     7561 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     7621 ggaagggcta attcactccc aacgaagata agatctgctt tttgcttgta ctgggtctct
     7681 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     7741 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     7801 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag
     7861 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga
     7921 gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     7981 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     8041 atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact
     8101 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     8161 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     8221 ctagactttt gcagagacca aattcgtaat catgtcatag ctgtttcctg tgtgaaattg
     8281 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     8341 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     8401 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     8461 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     8521 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     8581 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     8641 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     8701 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     8761 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     8821 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     8881 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     8941 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     9001 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     9061 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     9121 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     9181 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     9241 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     9301 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     9361 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     9421 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     9481 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     9541 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     9601 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     9661 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     9721 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     9781 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     9841 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     9901 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     9961 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
    10021 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
    10081 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
    10141 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
    10201 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
    10261 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
    10321 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
    10381 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
    10441 ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga
    10501 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
    10561 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa
    10621 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca
    10681 cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc tgcgcaactg
    10741 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg
    10801 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac
    10861 gacggccagt gccaagctg
//
