LOCUS V035052 10387 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V035052 VERSION V035052 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1804..1921 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2011..2214 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2317..4413 /label="Acsl1(NM_001302163)" /note="Acsl1(NM_001302163)" /gene="Acsl1" CDS 4420..4485 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" promoter 4529..4740 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 4753..5021 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" regulatory 5050..5059 /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" /regulatory_class="other" /label="Kozak sequence" CDS 5080..5742 /codon_start=1 /product="green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (Shagin et al., 2004)" /transl_table=1 /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA" /label="CopGFP" CDS 5812..5865 /codon_start=1 /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /product="2A peptide from Thosea asigna virus capsid protein" /protein_id="" /transl_table=1 /translation="EGRGSLLTCGDVEENPGP" /label="T2A" CDS 5866..6465 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 6466..7054 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 7128..7361 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 7433..7554 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 7594..7729 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(7762..7778) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 7786..7802 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(7810..7840) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 7855..7876 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(8164..8752) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(8923..9783) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(9784..9888) /gene="bla" /label="AmpR promoter" primer_bind 10362..10378 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg aagtccatga attgttccgg 2341 tattttcgaa tgccagagct gattgacatt cggcagtacg tgcgcaccct tccaaccaac 2401 accctcatgg ggtttggggc ttttgcagcg ctcaccacct tctggtatgc caccaggcct 2461 aaggccctga agccaccatg tgacctctcc atgcagtcag tggaaatagc gggtaccact 2521 gatggtattc gaagatcagc agtccttgaa gatgacaagc tcttggtgta ctactacgac 2581 gatgtcagaa ccatgtacga tggcttccag agggggattc aggtgtcaaa taatggtcct 2641 tgtttaggtt ctcggaagcc aaaccagccc tatgagtgga tttcctacaa agaggtggca 2701 gaactggctg agtgcatagg ctccgggctg atccagaagg ggttcaagcc ttgctccgag 2761 cagttcatcg gcctcttctc tcaaaacaga cccgagtggg tgatcgtcga gcaaggatgc 2821 ttctcttact caatggtggt cgtcccgctc tatgacaccc ttggagctga cgccatcacc 2881 tacatagtga acaaagctga actctctgtg atttttgctg acaagccaga aaaagccaaa 2941 ctcttattag aaggtgtaga aaacaagtta acaccatgcc ttaaaatcat agtcatcatg 3001 gactcctacg gcagtgatct ggtggaacga ggcaagaagt gtggggtgga aatcatcagc 3061 ctcaaagctc tggaggacct tggaagagtg aacagagtga agcccaagcc tccagaaccc 3121 gaagatcttg cgataatttg tttcacaagt ggaactacag gcaaccccaa aggagcaatg 3181 atcactcacc aaaacattat aaacgactgc tcaggtttta taaaagcaac agagagtgca 3241 ttcatcgctt ccacagatga tgtgctgata tctttcttgc ctctcgccca tatgtttgag 3301 accgttgtag agtgtgtaat gctgtgtcat ggagctaaga taggattttt ccaaggagat 3361 atcaggctgc ttatggacga cctcaaggtg cttcagccca ccatcttccc tgtggttccc 3421 aggctgctga accggatgtt cgacagaatt tttggacaag caaacacttc cttgaagcga 3481 tggctgttgg actttgcctc caaaaggaaa gaggcggagc ttcgcagtgg catcgtcaga 3541 aacaacagcc tgtgggataa actcatcttc cacaagatac agtcgagcct gggtgggaaa 3601 gtccggctga tgatcacagg agcagccccg gtgtctgcca cagtgctgac gtttctgagg 3661 acagcgctcg gctgccagtt ctatgaaggc tacggacaga ccgagtgcac tgctggttgc 3721 tgcctgagct tgcccggaga ctggacggca ggccatgttg gagcccccat gccttgcaat 3781 tatgtaaagc ttgtggatgt ggaagaaatg aattacctgg catccaaggg cgagggtgag 3841 gtgtgtgtga aaggggcaaa tgtgttcaaa ggctacttga aagacccagc aagaacagct 3901 gaagccctgg ataaagatgg ctggttacac acgggggaca ttggaaaatg gctgccaaat 3961 ggcaccttga agattatcga caggaaaaag cacatattta aactagccca aggagagtac 4021 atagcaccag aaaagattga aaatatctac ctgcggagtg aagccgtggc ccaggtgttt 4081 gtccacggag aaagcttgca ggcctttctc atagcagttg tggtacccga cgttgagagc 4141 ctaccgtcct gggcacagaa gagaggctta caagggtcct tcgaagaact gtgcaggaac 4201 aaggatatca ataaagctat cctggacgac ttgttgaaac ttgggaagga agccggtctg 4261 aagccatttg aacaggtcaa aggcattgct gtgcacccgg aattattttc tattgacaac 4321 ggccttctga ctccaacact gaaggcgaag aggccagagc tacggaacta tttcaggtcg 4381 cagatagatg aactgtacgc caccatcaag atcggatccg actacaaaga ccatgacggt 4441 gattataaag atcatgacat cgactacaag gatgacgatg acaagtagcg gccgcgaagg 4501 atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag 4561 aagttggggg gaggggtcgg caattgaacg ggtgcctaga gaaggtggcg cggggtaaac 4621 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat 4681 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag 4741 ctgaagcttc gaggggctcg catctctcct tcacgcgccc gccgccctac ctgaggccgc 4801 catccacgcc ggttgagtcg cgttctgccg cctcccgcct gtggtgcctc ctgaactgcg 4861 tccgccgtct aggtaagttt aaagctcagg tcgagaccgg gcctttgtcc ggcgctccct 4921 tggagcctac ctagactcag ccggctctcc acgctttgcc tgaccctgct tgctcaactc 4981 tacgtctttg tttcgttttc tgttctgcgc cgttacagat ccaagctgtg accggcgcct 5041 acgctagacg ccaccatgga gagcgacgag agcggcctgc ccgccatgga gatcgagtgc 5101 cgcatcaccg gcaccctgaa cggcgtggag ttcgagctgg tgggcggcgg agagggcacc 5161 cccaagcagg gccgcatgac caacaagatg aagagcacca aaggcgccct gaccttcagc 5221 ccctacctgc tgagccacgt gatgggctac ggcttctacc acttcggcac ctaccccagc 5281 ggctacgaga accccttcct gcacgccatc aacaacggcg gctacaccaa cacccgcatc 5341 gagaagtacg aggacggcgg cgtgctgcac gtgagcttca gctaccgcta cgaggccggc 5401 cgcgtgatcg gcgacttcaa ggtggtgggc accggcttcc ccgaggacag cgtgatcttc 5461 accgacaaga tcatccgcag caacgccacc gtggagcacc tgcaccccat gggcgataac 5521 gtgctggtgg gcagcttcgc ccgcaccttc agcctgcgcg acggcggcta ctacagcttc 5581 gtggtggaca gccacatgca cttcaagagc gccatccacc ccagcatcct gcagaacggg 5641 ggccccatgt tcgccttccg ccgcgtggag gagctgcaca gcaacaccga gctgggcatc 5701 gtggagtacc agcacgcctt caagaccccc atcgccttcg ccagatcccg cgctcagtcg 5761 tccaattctg ccgtggacgg caccgccgga cccggctcca ccggatctcg cgagggcaga 5821 ggaagtcttc taacatgcgg tgacgtggag gagaatcccg gccctatgac cgagtacaag 5881 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc 5941 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg 6001 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg 6061 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg 6121 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg 6181 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg 6241 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc 6301 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc 6361 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc 6421 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgaaatca acctctggat 6481 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt 6541 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc 6601 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg 6661 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc 6721 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa 6781 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat 6841 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc 6901 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt 6961 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag 7021 acgagtcgga tctccctttg ggccgcctcc ccgcctggta cctttaagac caatgactta 7081 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat 7141 tcactcccaa cgaagataag atctgctttt tgcttgtact gggtctctct ggttagacca 7201 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag 7261 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag 7321 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct 7381 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt 7441 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc 7501 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt 7561 ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc 7621 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc 7681 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct agacttttgc 7741 agagaccaaa ttcgtaatca tgtcatagct gtttcctgtg tgaaattgtt atccgctcac 7801 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt 7861 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc 7921 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg 7981 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 8041 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 8101 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 8161 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 8221 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 8281 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 8341 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg 8401 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 8461 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 8521 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 8581 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt 8641 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 8701 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 8761 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 8821 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt 8881 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag 8941 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt 9001 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc 9061 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc 9121 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg 9181 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac 9241 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg 9301 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc 9361 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact 9421 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc 9481 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat 9541 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc 9601 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac 9661 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa 9721 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact 9781 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg 9841 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg 9901 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag 9961 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca 10021 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc 10081 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc 10141 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag 10201 gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg 10261 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg 10321 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtgc 10381 caagctg //